Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,232,816	T→C	P384P (CCT→CCC)	fadH →	2,4‑dienoyl‑CoA reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,232,816	0	T	C	100.0%	177.3 / NA	51	P384P (CCT→CCC)	fadH	2,4‑dienoyl‑CoA reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/28);  total (23/28)

GGCCTGTCTCGATCAAATCTTCGTTGGCAAAGTCACCTCGTGCCTGGTGAATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCTGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTGGGCATCAGGTAACATTGTTTGACGCTCATAGCGAGATTGGCGGGCAGTTTAATATCGCCAAACAGATCCCCGGCAAAGAGGAGTTTTACGAAACGCTGC  >  NC_000913/3232684‑3232952                                                                                                                                     |                                                                                                                                         ggCCTGTCTCGATCAAATCTTCGTTGGCAAAGTCACCTCGTGCCTGGTGAATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCCgctgggc                                                                                                                                   <  2:524067/140‑1 (MQ=255)      gTCTCGATCAAATCTTCGTTGGCAAAGTCACCTCGTGCCTGGTGAATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCg                                                                                                                              >  1:772066/1‑140 (MQ=255)       tctcGATCAAATCTTCGTTGGCAAAGTCCCCTCGTGCCTGGTGAATCC‑CGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCCgctgggc                                                                                                                                   <  2:587245/133‑1 (MQ=255)       tctcGATCAAATCTTCGTTGGCAAAGTCACCTCGTGCCTGGTGAATCC‑CGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCCgctgggc                                                                                                                                   >  1:587245/1‑133 (MQ=255)            aTCAAATCTTCGTTGGCAAAGTCACCTCGTGCCTGGTGAATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCGTTTGcc                                                                                                                        >  2:982979/1‑140 (MQ=255)            aTCAAATCTTCGTTGGCAAAGTCACCTCGTGCCTGGTGAATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCGTTTGcc                                                                                                                        >  2:1013794/1‑140 (MQ=255)            aTCAAATCTTCGTTGGCAAAGTCACCTCGTGCCTGGTGAATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCGTTTGcc                                                                                                                        >  1:1170962/1‑140 (MQ=255)                 aTCTTCGTTGGCAAAGTCACCTCGTGCCTGGTGAATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCGTTTGCCATTaa                                                                                                                   >  2:1158559/1‑140 (MQ=255)                 aTCTTCGTTGGCAAAGTCACCTCGTGCCTGGTGAATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCGTTTGCCATTaa                                                                                                                   >  1:1156276/1‑140 (MQ=255)                 aTCTTCGTTGGCAAAGTCACCTCGTGCCTGGTGAATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCGTTTGCCATTaa                                                                                                                   >  2:192322/1‑140 (MQ=255)                 aTCTTCGTTGGCAAAGTCACCTCGTGCCTGGTGAATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCGTTTGCCATTaa                                                                                                                   >  1:1168233/1‑140 (MQ=255)                 aTCTTCGTTGGCAAAGTCACCTCGTGCCTGGTGAATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCGTTTGCCATTaa                                                                                                                   >  2:88333/1‑140 (MQ=255)                 aTCTTCGTTGGCAAACTCACCTCGTGCCTGGTGATTCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCGGTTGCCATTaa                                                                                                                   >  2:439284/1‑140 (MQ=255)                     tCGTTGGCAAAGTCACCTCGTGCCTGGTGAATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCGTTTGCCATTAAcgcg                                                                                                               >  2:47387/1‑140 (MQ=255)                                 tCACCTCGTGCCTGGTGAATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTggg                                                                                                   <  2:1168233/140‑1 (MQ=255)                                   cccTCGTGCCTGGTGAATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTGGGCa                                                                                                 <  1:47387/139‑1 (MQ=255)                                   cccTCGTGCCTGGTGAATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTGGGCa                                                                                                 <  1:88333/139‑1 (MQ=255)                                   cccTCGTGCCTGGTGAATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTGGGCa                                                                                                 <  2:693368/139‑1 (MQ=255)                                   aCCTCGTGCCTGGTGAATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTGGGCa                                                                                                 <  1:468312/140‑1 (MQ=255)                                   aCCTCGTGCCTGGTGAATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTGGGCa                                                                                                 <  2:1039411/140‑1 (MQ=255)                                   aCCTCGTGCCTGGTGAATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTGGGCa                                                                                                 <  1:489300/140‑1 (MQ=255)                                           ccTGGTGAATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTGGGCa                                                                                                 >  2:507862/1‑132 (MQ=255)                                           ccTGGTGAATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTGGGCa                                                                                                 <  1:507862/132‑1 (MQ=255)                                               gTGAATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTGGGCATCAGGTAACAtt                                                                                     >  2:418342/1‑140 (MQ=255)                                                  aaTCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTGGGCATCAGGTAACATTGtt                                                                                  >  1:336238/1‑140 (MQ=255)                                                   aTCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTGGGCATc                                                                                               >  1:876916/1‑126 (MQ=255)                                                   aTCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTGGGCATc                                                                                               <  2:876916/126‑1 (MQ=255)                                                     ccTCGCGCCTGCCACGAAACCAAAATGCCAATCCTCCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTGGGCATCAGGTAACATTGTTTGa                                                                               <  2:480128/140‑1 (MQ=255)                                                         gcgcCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGAAAAAGAATCTGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTGGGCATCAGGTAACATTGTTTGACGCt                                                                           >  2:522653/1‑140 (MQ=255)                                                         gcgcCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTGGGCATCAGGTAACATTGTTTGACGCt                                                                           <  2:1013034/140‑1 (MQ=255)                                                         gcgcCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTGGGCATCAGGTAACATTGTTTGACGCt                                                                           <  1:876769/140‑1 (MQ=255)                                                         gcgcCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTGGGCATCAGGTAACATTGTTTGACGCt                                                                           >  1:581978/1‑140 (MQ=255)                                                          cgcCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTGGGCATCAGGTAACATTGTTTGACGCTc                                                                          >  2:546705/1‑140 (MQ=255)                                                              tGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTGGGCATCAGGTAACATTGTTTGACGCTCATAg                                                                      >  1:422357/1‑140 (MQ=255)                                                                        ccAAAATGCCAATCCTTCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTGGGCATCAGGTAACATTGTTTGACGCTCATAGCGAGATTGGc                                                            <  1:982979/140‑1 (MQ=255)                                                                         cAAAATGCCAATCCTTCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTGGGCATCAGGTAACATTGTTTGACGCTCATAGCGAGATTGGCg                                                           <  2:336238/140‑1 (MQ=255)                                                                           aaaTGCCAATCCTTCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTGGGCATCAGGTAACATTGTTTGACGCTCATAGCGAGATTGGCggg                                                         <  1:1158559/140‑1 (MQ=255)                                                                           aaaTGCCAATCCTTCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTGGGCATCAGGTAACATTGTTTGACGCTCATAGCGAGATTGGCggg                                                         <  1:874922/140‑1 (MQ=255)                                                                           aaaTGCCAATCCTTCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTGGGCATCAGGTAACATTGTTTGACGCTCATAGCGAGATTGGCggg                                                         <  1:587010/140‑1 (MQ=255)                                                                                     ccTTCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTGGGCATCAGGTAACATTGTTTGACGCTCATAGCGAGATTGGCGGGCAGTTTAata                                               <  2:1170962/140‑1 (MQ=255)                                                                                               gTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTGGGCATCAGGTAACATTGTTTGACGCTCATAGCGAGATTGGCGGGCAGGTTAATATCGCCAAACa                                     >  2:437786/1‑140 (MQ=255)                                                                                                tGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTGGGCATCAGGTAACATTGTTTGACGCTCATAGCGAGATTGGCGGGCAGTTTaa                                                 >  2:22905/1‑127 (MQ=255)                                                                                                tGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTGGGCATCAGGTAACATTGTTTGACGCTCATAGCGAGATTGGCGGGCAGTTTaa                                                 <  1:22905/127‑1 (MQ=255)                                                                                                     aaaaaaaaTCTGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTGGGCATCAGGTAACATTGTTTGACGCTCATAGCGAGATTGGCGGGCAGTTTAATATCGCCAAACAGATccc                               <  1:192322/140‑1 (MQ=255)                                                                                                       aaaaaaTCTGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTGGGCATCAGGTAACATTGTTTGACGCTCATAGCGAGATTGGCGGGCAGTTTAATATCGCCAAACAGATCCCCg                             >  1:397611/1‑140 (MQ=255)                                                                                                       aaaaaaTCTGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTGGGCATCAGGTAACATTGTTTGACGCTCATAGCGAGATTGGCGGGCAGTTTAATATCGCCAAACAGATCCCCg                             <  2:645481/140‑1 (MQ=255)                                                                                                             tCTGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTGGGCATCAGGTAACATTGTTTGACGCTCATAGCGAGATTGGCGGGCAGTTTAATATCGCCAAACAGATCCCCGGCAAag                       <  1:522653/140‑1 (MQ=255)                                                                                                               tGGCGGTGGTCGGTGCGGGACCCGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTGGGCATCAGGTAACATTGTTTGACGCTCATAGCGAGATTGGCGGGCAGTTTAATATCGCCAAACAGATCCCCGGCAAagag                     <  1:117323/140‑1 (MQ=255)                                                                                                                               gggACCCGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTGGGCATCAGGTAACATTGTTTGACGCTCATAGCGAGATTGGCGGGCAGTTTAATATCGCCAAACAGATCCCCGGCAAAGAGGAGTTTTACGAAACGc     <  2:581978/140‑1 (MQ=255)                                                                                                                                 gACCCGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTGGGCATCAGGTAACATTGTTTGACGCTCATAGCGAGATTGGCGGGCAGTTTAATATCGCCAAACAGATCCCCGGCAAAGAGGAGTTTTACGAAACGCTg   <  2:1156276/140‑1 (MQ=255)                                                                                                                                  aCCCGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTGGGCATCAGGTAACATTGTTTGACGCTCATAGCGAGATTGGCGGGCAGTTTAATATCGCCAAACAGATCCCCGGCAAAGAGGAGTTTTACGAAACGCTgc  <  1:418342/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                         GGCCTGTCTCGATCAAATCTTCGTTGGCAAAGTCACCTCGTGCCTGGTGAATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCTGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTGGGCATCAGGTAACATTGTTTGACGCTCATAGCGAGATTGGCGGGCAGTTTAATATCGCCAAACAGATCCCCGGCAAAGAGGAGTTTTACGAAACGCTGC  >  NC_000913/3232684‑3232952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑