Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,295,086	G→A	G563S (GGT→AGT)	lpoA →	outer membrane lipoprotein ‑ activator of MrcA activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,295,086	0	G	A	100.0%	185.0 / NA	52	G563S (GGT→AGT)	lpoA	outer membrane lipoprotein ‑ activator of MrcA activity
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/31);  total (21/31)

GCTGCAAACCACGCCAACCGATGACCAGTTCACCAATAATGGCGGTCGTGTCGATGCGGTGTACATTGTGGCAACGCCGGGTGAAATCGCTTTTATCAAACCGATGATCGCCATGCGTAACGGTAGCCAGAGCGGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTGGCCCGGATTTCCGACTGGAGATGGAAGGCTTGCAGTACAGCGAAATCCCGATGCTGGCAGGCGGTAATCTACCGTTAATGCAGCAG  >  NC_000913/3294953‑3295217                                                                                                                                      |                                                                                                                                    gCTGCAAACCACGCCAACCGATGACCAGTTCACCAATAATGGCGGTCGTGTCGATGCGGTGTACATTGTGGCAACGCCGGGTGAAATCGCTTTTATCAAACCGATGATCGCCATGCGTAACGGTAGCCAGAGCAGTGCaa                                                                                                                               <  1:748968/140‑1 (MQ=255) gCTGCAAACCACGCCAACCGATGACCAGTTCACCAATAATGGCGGTCGTGTCGATGCGGTGTACATTGCGGCAACGCCGGGTGAAATCGCTTTTATCAAACCGATGATCGCCATGCGTAACGGTAGCCAGAGCAGTGCaa                                                                                                                               <  1:406771/140‑1 (MQ=255)   tGCAAACCACGCCAACCGATGACCAGTTCACCAATAATGGCGGTCGTGTCGATGCGGTGTACATTGTGGCAACGCCGGGTGAAATCGCTTTTATCAAACCGATGATCGCCATGCGTAACGGTAGCCAGAGCAGTGCAACg                                                                                                                             >  1:406965/1‑140 (MQ=255)    gCAAACCACGCCAACCGATGACCAGTTCACCAATAATGGCGGTCGTGTCGATGCGGTGTACA‑TGTGGCAACGCCGGGTGAAATCGCTTTTATCAAACCGATGATCGCCATGCGTAACGGTAGCCAGAGCAGTGCAACGCt                                                                                                                           <  2:481377/140‑1 (MQ=255)            cGCCAACCGATGACCAGTTCACCAATAATGGCGGTCGTGTCGATGCGGTGTACATTGTGGCAACGCCGGGTGAAATCGCTTTTATCAAACCGATGATCGCCATGCGTAACGGTAGCCAGAGCAGTGCAACGCTGTACGcc                                                                                                                    <  2:301123/140‑1 (MQ=255)               cAACCGATGACCAGTTCACCAATAATGGCGGTCGTGTCGATGCGGTGTACATTGTGGCAACGCCGGGTGAAATCGCTTTTATCAAACCGATGATCGCCATGCGTAACGGTAGCCAGAGCAGTGCAACGCTGTACGCCAGc                                                                                                                 <  1:391017/140‑1 (MQ=255)                 aCCGATTACCAGTTCACCAATAATGGCGGTCGTGTCGATGCGGTGTACATTGTGGCAACGCCGGGTGAAATCGCTTTTATCAAACCGATGATCGCCATGCGTAACGGTAGCCAGAGCAGTGCAACGCTGTACg                                                                                                                      >  1:1133486/1‑133 (MQ=255)                 aCCGATTACCAGTTCACCAATAATGGCGGTCGTGTCGATGCGGTGTACATTGTGGCAACGCCGGGTGAAATCGCTTTTATCAAACCGATGATCGCCATGCGTAACGGTAGCCAGAGCAGTGCAACGCTGTACg                                                                                                                      <  2:1133486/133‑1 (MQ=255)                  ccGATGACCAGTTCACCAATAATGGCGGTCGTGTCGATGCGGGGTACATTGTGGCAACGCCGGGGGAAATCGCTTTTTTCAAAACGATGATCGCCCTGCGTAACGGGAGCCAGAGCAGTGCAACGCTGTACGCCaccccc                                                                                                              >  2:391017/1‑135 (MQ=255)                    gATGACCAGTTCACCAATAATGGCGGTCGTGTCGATGCGGTGTACATTGTGGCAACGCCGGGTGAAATCGCTTTTATCAAACCGATGATCGCCATGCGTAACGGTAGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCg                                                                                                            <  1:209720/140‑1 (MQ=255)                                aCCAATAATGGCGGTCGTGTCGATGCGGTGTACATTGTGGCAACGCCGGGTGAAATCGCTTTTATCAAACCGATGATCGCCATGCGTAACGGTAGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAgg                                                                                                >  2:377281/1‑140 (MQ=255)                                  cAATAATGGCGGTCGTGTCGATGCGGGGTACATTGTGGCAACGCCGGGGGAAATCGCTTTTATCAAACCGATGATCGCCATGCGTAACGGTAGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGa                                                                                              >  1:41270/1‑140 (MQ=255)                                     taatGGCGGTCGTGTCGATGCGGTGTACATTGTGGCAACGCCGGGTGAAATCGCTTTTATCAAACCGATGATCGCCATGCGTAACGGTAGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCg                                                                                           <  1:74838/140‑1 (MQ=255)                                        tGGCGGTCGTGTCGATGCGGTGTACATTGTGGCAACGCCGGGTGAAATCGCTTTTATCAAACCGATGATCGCCATGCGTAACGGTAGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTg                                                                                        <  2:15270/140‑1 (MQ=255)                                          gCGGTCGTGTCGATGCGGTGTACATTGTGGCAACGCCGGGTGAAATCGCTTTTATCAAACCGATGATCGCCATGCGTAACGGTAGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTGGc                                                                                      <  1:156822/140‑1 (MQ=255)                                            ggTCGTGTCGATGCGGGGTACATTTTGGCAACGCCGGGTGAAATCGCTTTTATCAAACCGATGATCGCCATGCGTAACGGTAGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTGGccc                                                                                    >  2:96177/1‑140 (MQ=255)                                                gtgtCGATGCGGGGTACATTGTTGCCACGCCGGGGGGAAACGCTTTTTTCAAACCCATGATCGCCATGCGTAAAGGGAGCCCGAGCAGTGCAACGCTGTACGCCAGCCCCCGCCGTGCGCAAAGGGCCGCTGGCCCGGAt                                                                                >  2:1113864/1‑140 (MQ=255)                                                       tGCGGTGTACATTGTGGCAACGCCGGGTGAAATCGCTTTTATCAAACCGATGATCGCCATGCGTAACGGTAGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTGGCCCGGATTTCCGAc                                                                         <  1:61842/140‑1 (MQ=255)                                                           gtgtACATTGTGGCAACGCCGGGTGAAATCGCTTTTATCAAACCGATGATCGCCATGCGTAACGGTAGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTGGCCCGGATTTCCGACTGga                                                                     >  2:143439/1‑140 (MQ=255)                                                                   tgtgGCAACGCCGGGTGAAATCGCTTTTATCAAACCGATGATCGCCATGCGTAACGGTAGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTGGCCCGGATTTCTGACTGGAGATGGAAg                                                             <  1:724410/140‑1 (MQ=255)                                                                   tgtgGCAACGCCGGGTGAAATCGCTTTTATCAAACCGATGATCGCCATGCGTAACGGTAGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTGGCCCGGATTTCCGACTGGAGATGGAAg                                                             <  2:989996/140‑1 (MQ=255)                                                                   tgtgGCAACGCCGGGTGAAATCGCTTTTATCAAACCGATGATCGCCATGCGTAACGGTAGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTGGCCCGGATTTCCGACTGGAGATGGAAg                                                             <  2:595502/140‑1 (MQ=255)                                                                   tgtgGCAACGCCGGGTGAAATCGCTTTTATCAAACCGATGATCGCCATGCGTAACGGTAGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTGGCCCGGATTTCCGACTGGAGATGGAAg                                                             <  1:519481/140‑1 (MQ=255)                                                                   tgtgGCAACGCCGGGTGAAATCGCTTTTATCAAACCGATGATCGCCATGCGTAACGGTAGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTGGCCCGGATTTCCGACTGGAGATGGAAg                                                             <  2:400331/140‑1 (MQ=255)                                                                   tgtgGCAACGCCGGGTGAAATCGCTTTTATCAAACCGATGATCGCCATGCGTAACGGTAGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTGGCCCGGATTTCCGACTGGAGATGGAAg                                                             >  1:400331/1‑140 (MQ=255)                                                                    gtgGCAACGCCGGGTGAAATCGCTTTTATCAAACCGATGATCGCCATGCGTAACGGTAGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTGGCCCGGATTTCCGACTGGAGATGGAAgg                                                            >  1:498412/1‑140 (MQ=255)                                                                     tgGCAACGCCGGGTGAAATCGCTTTTATCAAACCGATGATCGCCATGCGTAACGGTAGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTGGCCCGGATTTCCGACTGGAGATGGAAGGc                                                           >  2:274194/1‑140 (MQ=255)                                                                     tgGCAACGCCGGGTGAAATCGCTTTTATCAAACCGATGATCGCCATGCGTAACGGTAGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTGGCCCGGATTTCCGACTGGAGATGGAAGGc                                                           >  1:303823/1‑140 (MQ=255)                                                                      ggCAACGCCGGGTGAAATCGCTTTTATCAAACCGATGATCGCCATGCGTAACGGTAGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTGGCCCGGATTTCCGACTGGAGATgg                                                                <  1:135338/134‑1 (MQ=255)                                                                      ggCAACGCCGGGTGAAATCGCTTTTATCAAACCGATGATCGCCATGCGTAACGGTAGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTGGCCCGGATTTCCGACTGGAGATgg                                                                >  2:135338/1‑134 (MQ=255)                                                                      ggCAACGCCGGGTGAAATCGCTTTTATCAAACCGATGATCGCCATGCGTAACGGTAGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTGGCCCGGATTTCCGACTGGAGATGGAAGGCt                                                          >  1:271174/1‑140 (MQ=255)                                                                      ggCAACGCCGGGTGAAATCGCTTTTATCAAACCGATGATCGCCATGCGTAACGGTAGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTGGCCCGGATTTCCGACTGGAGATGGAAGGCt                                                          >  2:603174/1‑140 (MQ=255)                                                                      ggCAACGCCGGGTGAAATCGCTTTTATCAAACCGATGATCGCCATGCGTAACGGTAGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTGGCCCGGATTTCCGACTGGAGATGGAAGGCt                                                          >  1:629221/1‑140 (MQ=255)                                                                        cAACGCCGGGTGAAATCGCTTTTATCAAACCGATGATCGCCATGCGTAACGGTAGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTGGCCCGGATTTCCGACTGGAGATGGAAGGCTTg                                                        <  1:377281/140‑1 (MQ=255)                                                                        cAACGCCGGGTGAAATCGCTTTTATCAAACCGATGATCGCCATGCGTAACGGTAGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTGGCCCGGATTTCCGACTGGAGATGGAAGGCTTg                                                        >  1:81226/1‑140 (MQ=255)                                                                          aCGCCGGGTGAAATCGCTTTTATCAAACCGATGATCGCCATGCGTAACGGTAGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTGGCCCGGATTTCCGACTGGAGATGGAAGGCTTGCa                                                      <  2:1073725/140‑1 (MQ=255)                                                                          aCGCCGGGTGAAATCGCTTTTATCAAACCGATGATCGCCATGCGTAACGGTAGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTGGCCCGGATTTCCGACTGGAGATGGAAGGCTTGCa                                                      >  1:494912/1‑140 (MQ=255)                                                                                 gTGAAATCGCTTTTATCAAACCGATGATCGCCATGCGTAACGGTAGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTGGCCCGGATTTCCGACTGGAGATGGAAGGCTTGCAGTACAGc                                               >  2:862046/1‑140 (MQ=255)                                                                                             ttATCAAACCGATGATCGCCATGCGTAACGGTAGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTGGCCCGGATTTCCGACTGGAGATGGAAGGCTTGCAGTACAGCGAAATCCCGATg                                   <  2:303823/140‑1 (MQ=255)                                                                                             ttATCAAACCGATGATCGCCATGCGTAACGGTAGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTGGCCCGGATTTCCGACTGGAGATGGAAGGCTTGCAGTACAGCGAAATCCCGATg                                   <  2:406965/140‑1 (MQ=255)                                                                                                 cAAACCGATGATCGCCATGCGTAACGGTAGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTGGCCCGGATTTCCGACTGGAGATGGAAGGCTTGCAGTACAGCGAAATCCCGATGCTgg                               <  2:629221/140‑1 (MQ=255)                                                                                                   aaCCGATGATCGCCATGCGTAACGGTAGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTGGCCCGGATTTCCGACTGGAGATGGAAGGCTTGCAGTACAGCGAAATCCCGATGCTGGCa                             <  2:494912/140‑1 (MQ=255)                                                                                                         tgatCGCCATGCGTAACGGTAGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTGGCCCGGATTTCCGACTGGAGATGGAAGGCTTGCAGTACAGCGAAATCCCGATGCTGGCAGGCGGt                       <  1:427889/140‑1 (MQ=255)                                                                                                         tgatCGCCATGCGTAACGGTAGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTGGCCCGGATTTCCGACTGGAGAGGGAAGGCTTGCAGTACAGCGAAATCCCGATGCTGGCAGGCGGt                       >  2:1085844/1‑140 (MQ=255)                                                                                                          gatCGCCATGCGTAACGGTAGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTGGCCCGGATTTCCGACTGGAGATGGAAGGCTTGCAGTACAGCGAAATCCCGATGCTGGCAGGCGGTa                      <  1:377485/140‑1 (MQ=255)                                                                                                            tCGCCATGCGTAACGGTAGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTGGCCCGGATTTCCGACTGGAGATGGAAGGCTTGCAGTACAGCGAAATCCCGATGCTGGCAGGCGGTAAt                    <  2:81226/140‑1 (MQ=255)                                                                                                             cGCCATGCGTAACGGTAGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTGGCCCGGATTTCCGACTGGAGATGGAAGGCTTGCAGTACAGCGGAATCCCGATGCTGGCAGGCGGTAATc                   >  1:818152/1‑140 (MQ=255)                                                                                                             cGCCATGCGTAACGGTAGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTGGCCCGGATTTCCGACTGGAGATGGAAGGCTTGCAGTACAGCGAAATCCCGATGCTGGCAGGCGGTAATc                   <  1:580885/140‑1 (MQ=255)                                                                                                              gCCATGCGTAACGGTAGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTGGCCCGGATTTCCGACTGGAGATGGAAGGCTTGCAGTACAGCGAAATCCCGATGCTGGCAGGCGGTAATCt                  <  1:274194/140‑1 (MQ=255)                                                                                                                      tAACGGTAGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTGGCCCGGATTTCCGACTGGAGATGGAAGGCTTGCAGTACAGCGAAATCCCGATGCTGGCAGGCGGTAATCTACCGTTaa          <  2:498412/140‑1 (MQ=255)                                                                                                                        aCGGTAGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTGGCCCGGATTTCCGACTGGAGATGGAAGGCTTGCAGTACAGCGAAATCCCGATGCTGGCAGGCGGTAATCTACCGTTAATg        >  2:870666/1‑140 (MQ=255)                                                                                                                         cGGTAGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTGGCCCGGATTTCCGACTGGAGATGGAAGGCTTGCAGTACAGCGAAATCCCGATGCTGGCAGGCGGTAATCTACCGTTAATgc       <  2:934552/140‑1 (MQ=255)                                                                                                                            tAGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTGGCCCGGATTTCCGACTGGAGATGGAAGGCTTGCAGTACAGCGAAATCCCGATGCTGGCAGGCGGTAATCTACCGTTAATgcagc    <  1:96177/140‑1 (MQ=255)                                                                                                                             aGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTGGCCCGGATTTCCGACTGGAGATGGAAGGCTTGCAGTACAGCGAAATCCCGATGCTGGCAGGCGGTAATCTACCGTTAATgcagca   >  2:603512/1‑140 (MQ=255)                                                                                                                             aGCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTGGCCCGGATTTCCGACTGGAGATGGAAGGCTTGCAGTACAGCGAAATCCCGATGCTGGCAGGCGGTAATCTACCGTTAATgcagca   >  1:40828/1‑140 (MQ=255)                                                                                                                              gCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTGGCCCGGATTTCCGACTGGAGATGGAAGGCTTGCAGTACAGCGAAATCCCGATGCTGGCAGGCGGTAATCTACCGTTAATGcagcag  >  1:78421/1‑140 (MQ=255)                                                                                                                              gCCAGAGCAGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTGGCCCGGATTTCCGACTGGAGATGGAAGGCTTGCAGTACAGCGAAATCCCGATGCTGGCAGGCGGTAATCTACCGTTAATGcagcag  >  2:145713/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                    GCTGCAAACCACGCCAACCGATGACCAGTTCACCAATAATGGCGGTCGTGTCGATGCGGTGTACATTGTGGCAACGCCGGGTGAAATCGCTTTTATCAAACCGATGATCGCCATGCGTAACGGTAGCCAGAGCGGTGCAACGCTGTACGCCAGCTCCCGCAGTGCGCAAGGGACCGCTGGCCCGGATTTCCGACTGGAGATGGAAGGCTTGCAGTACAGCGAAATCCCGATGCTGGCAGGCGGTAATCTACCGTTAATGCAGCAG  >  NC_000913/3294953‑3295217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑