Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,394,995	C→T	V422I (GTT→ATT)	yhdP ←	AsmA2 domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,394,995	0	C	T	100.0%	236.7 / NA	66	V422I (GTT→ATT)	yhdP	AsmA2 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/31);  total (35/31)

ATCCAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAACGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATCGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCGGGATATCCAGCGCCAGAGTG  >  NC_000913/3394858‑3395128                                                                                                                                          |                                                                                                                                      aTCCAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGc                                                                                                                                     >  1:207446/1‑140 (MQ=255)  tCCAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCt                                                                                                                                    >  1:699362/1‑140 (MQ=255)        tGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGa                                                                                                                              <  2:948026/140‑1 (MQ=255)               cTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTc                                                                                                                       <  1:1035342/140‑1 (MQ=255)                tttGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTcc                                                                                                                      >  1:853226/1‑140 (MQ=255)                  tGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCg                                                                                                                    >  1:232686/1‑140 (MQ=255)                       ttGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGaa                                                                                                               <  2:494610/140‑1 (MQ=255)                         gTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACAATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAgt                                                                                                             >  1:675547/1‑140 (MQ=255)                            cagccagcTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGtt                                                                                                          >  1:1073959/1‑140 (MQ=255)                               ccagcTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCg                                                                                                       >  2:1070461/1‑140 (MQ=255)                               ccagcTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACAATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCg                                                                                                       <  2:675547/140‑1 (MQ=255)                                 agcTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCa                                                                                                     <  2:925196/140‑1 (MQ=255)                                 agcTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCa                                                                                                     <  2:848569/140‑1 (MQ=255)                                 agcTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCa                                                                                                     <  2:207446/140‑1 (MQ=255)                                 agcTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCa                                                                                                     <  1:1038992/140‑1 (MQ=255)                                      ataGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCgg                                                                                                <  2:232686/140‑1 (MQ=255)                                             ccTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCa                                                                                                     <  1:621562/128‑1 (MQ=255)                                             ccTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCa                                                                                                     >  2:621562/1‑128 (MQ=255)                                             ccTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTaataat                                                                                         >  1:830619/1‑140 (MQ=255)                                             ccTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTaataat                                                                                         >  1:738435/1‑140 (MQ=255)                                             ccTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATC‑TTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAAtt                                                                                        >  1:103132/1‑140 (MQ=255)                                               tGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAAttt                                                                                       >  2:572264/1‑140 (MQ=255)                                                 gCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTcc                                                                                     >  1:850845/1‑140 (MQ=255)                                                    gTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCAtt                                                                                  <  2:310160/140‑1 (MQ=255)                                                    gTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCAtt                                                                                  >  1:41352/1‑140 (MQ=255)                                                         cGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTc                                                                             <  2:738435/140‑1 (MQ=255)                                                            tttCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAg                                                                          >  1:988018/1‑140 (MQ=255)                                                                  gTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGa                                                                    <  2:486692/140‑1 (MQ=255)                                                                   tGGCGCACGGAATACCGTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGAt                                                                   <  1:572264/140‑1 (MQ=255)                                                                   tGGCGCACGGAATACCGTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGAt                                                                   <  2:41352/140‑1 (MQ=255)                                                                    ggCGCACGGAATACCGTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATc                                                                  <  2:699362/140‑1 (MQ=255)                                                                     gcgcACGGAATACCGTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATCg                                                                 >  1:415740/1‑140 (MQ=255)                                                                      cgcACGGAATACCGTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATCGc                                                                >  2:238908/1‑140 (MQ=255)                                                                      cgcACGGAATACCGTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATCGc                                                                >  2:707838/1‑140 (MQ=255)                                                                        cACGGAATACCGTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATCGCTc                                                              >  2:327819/1‑140 (MQ=255)                                                                             aaTACCGTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATCGCTCCACGa                                                         <  2:853226/140‑1 (MQ=255)                                                                                aCCGTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATCGCTCCACGATGc                                                      >  1:1107056/1‑140 (MQ=255)                                                                                aCCGTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATCGCTCCACGATGc                                                      <  2:660984/140‑1 (MQ=255)                                                                                aCCGTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTCCCAGGCCAGATCGCTCCACGATGc                                                      >  1:825087/1‑140 (MQ=255)                                                                                       cGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATCGCTCCACGATGCCTGaaaa                                               >  2:398289/1‑140 (MQ=255)                                                                                       cGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATCGCTCCACGATGCCTGaaaa                                               >  2:319697/1‑140 (MQ=255)                                                                                       cGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATCGCTCCACGATGCCTGaaaa                                               >  1:1018253/1‑140 (MQ=255)                                                                                       cGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATCGCTCCACGATGCCTGaaaa                                               >  2:590202/1‑140 (MQ=255)                                                                                       cGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATCGCTCCACGATGCCTGaaaa                                               >  2:1117697/1‑140 (MQ=255)                                                                                       cGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATCGCTCCACGATGCCTGaaaa                                               >  1:265115/1‑140 (MQ=255)                                                                                       cGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATCGCTCCACGATGCCTGaaaa                                               >  1:574854/1‑140 (MQ=255)                                                                                       cGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATCGCTCCACGATGCCTGaaaa                                               >  1:7000/1‑140 (MQ=255)                                                                                       cGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATCGCTCCACGATGCCTGaaaa                                               >  1:759304/1‑140 (MQ=255)                                                                                        gTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATCGCTCCACGATGCCTGAAAAc                                              <  1:707838/140‑1 (MQ=255)                                                                                          aaGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATCGCTCCACGATGCCTGAAAACgg                                            <  1:327819/140‑1 (MQ=255)                                                                                                    tGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATCGCTCCACGATGCCTGAAAACGGGTCTTGTCTg                                  <  1:145212/140‑1 (MQ=255)                                                                                                          cTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATCGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCt                            >  2:965197/1‑140 (MQ=255)                                                                                                          cTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATCGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCt                            >  1:828645/1‑140 (MQ=255)                                                                                                          cTTCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATCGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCt                            >  1:1005575/1‑140 (MQ=255)                                                                                                           ttCATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATCGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTg                           <  2:759304/140‑1 (MQ=255)                                                                                                             cATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATCGCTCCACGATGCCTGAAAATGGGTCTTGTCTGCCGCCTGaa                         <  2:830619/140‑1 (MQ=255)                                                                                                             cATCGACGCGGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATCGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGaa                         <  1:238908/140‑1 (MQ=255)                                                                                                                   cgcgGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATCGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCGGGa                   <  2:7000/140‑1 (MQ=255)                                                                                                                   cgcgGTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATCGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCGGGa                   <  2:265115/140‑1 (MQ=255)                                                                                                                       gTAAGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATCGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCGGGATATc               <  1:398289/140‑1 (MQ=255)                                                                                                                          aGCAAACCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATCGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCGGGATATCCAg            <  2:103132/140‑1 (MQ=255)                                                                                                                               aCCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATCGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCGGGATATCCAGCGCCa       <  1:590202/140‑1 (MQ=255)                                                                                                                               aCCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATCGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCGGGATATCCAGCGCCa       <  1:319697/140‑1 (MQ=255)                                                                                                                               aCCATTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATCGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCGGGATATCCAGCGCCa       <  2:1005575/140‑1 (MQ=255)                                                                                                                                  aTTTTCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATCGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCGGGATATCCAGCGCCagag    >  2:1052146/1‑140 (MQ=255)                                                                                                                                    tttCAATGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATCGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCGGGATATCCAGCGCCAGAgtg  >  1:764871/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      ATCCAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAACGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATCGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCGGGATATCCAGCGCCAGAGTG  >  NC_000913/3394858‑3395128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑