Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,450,057	T→C	E59E (GAA→GAG)	rplV ←	50S ribosomal subunit protein L22

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,450,057	0	T	C	100.0%	208.4 / NA	59	E59E (GAA→GAG)	rplV	50S ribosomal subunit protein L22
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/30);  total (29/30)

ATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCT  >  NC_000913/3449926‑3450190                                                                                                                                    |                                                                                                                                      aTGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGtt                                                                                                                               >  1:481140/1‑140 (MQ=255)  tgtgGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTa                                                                                                                              >  1:883057/1‑140 (MQ=255)   gtgGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAg                                                                                                                             >  1:1164999/1‑140 (MQ=255)    tgGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGc                                                                                                                            >  2:739987/1‑140 (MQ=255)     ggCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCa                                                                                                                           <  2:394613/140‑1 (MQ=255)     ggCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCa                                                                                                                           <  1:902609/140‑1 (MQ=255)         ggTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATgg                                                                                                                       >  2:959809/1‑140 (MQ=255)         ggTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATgg                                                                                                                       >  2:387626/1‑140 (MQ=255)           tGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATGGCa                                                                                                                     <  2:481140/140‑1 (MQ=255)           tGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATGGCa                                                                                                                     <  1:589235/140‑1 (MQ=255)                ttCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTc                                                                                                                >  1:838749/1‑140 (MQ=255)                     gATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGaa                                                                                                           >  2:123285/1‑140 (MQ=255)                     gATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGaa                                                                                                           <  2:794635/140‑1 (MQ=255)                     gATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGAGCTCAGCGTTAGCAATGGCAGATTCCAGaa                                                                                                           >  2:344092/1‑140 (MQ=255)                       tGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACt                                                                                                         >  1:451033/1‑140 (MQ=255)                          gATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTc                                                                                                      >  1:175257/1‑140 (MQ=255)                          gATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTc                                                                                                      <  2:524914/140‑1 (MQ=255)                          gATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTc                                                                                                      <  2:509951/140‑1 (MQ=255)                          gATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTc                                                                                                      <  2:883057/140‑1 (MQ=255)                               gCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGAc                                                                                                 <  2:271074/140‑1 (MQ=255)                                cACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGAcc                                                                                                <  1:689582/140‑1 (MQ=255)                                       ttttGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAg                                                                                         <  2:477025/140‑1 (MQ=255)                                               gcgGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTc                                                                                 >  1:382078/1‑140 (MQ=255)                                               gcgGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTc                                                                                 >  1:759899/1‑140 (MQ=255)                                               gcgGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTc                                                                                 >  2:874776/1‑140 (MQ=255)                                               gcagCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTc                                                                                 >  1:633756/4‑140 (MQ=255)                                                   cATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCttt                                                                                  >  1:118748/1‑135 (MQ=255)                                                   cATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCttt                                                                                  <  2:118748/135‑1 (MQ=255)                                                   cATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCttgt                                                                             <  2:796159/140‑1 (MQ=255)                                                   cATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCttgt                                                                             <  1:1001355/140‑1 (MQ=255)                                                        tGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGgtg                                                                        <  1:476931/140‑1 (MQ=255)                                                        tGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGgtg                                                                        <  2:1164999/140‑1 (MQ=255)                                                         gcgcTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGgtgt                                                                       >  1:712776/1‑140 (MQ=255)                                                            cTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAgg                                                                    <  2:759899/140‑1 (MQ=255)                                                              tCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGTc                                                                  >  1:131720/1‑140 (MQ=255)                                                                  gCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGTCaaaa                                                              <  2:84051/140‑1 (MQ=255)                                                                      gggCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGTCAAAATATc                                                          <  1:739987/140‑1 (MQ=255)                                                                           ttCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGTCaaaa                                                              >  2:1164109/1‑131 (MQ=255)                                                                           ttCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGTCaaaa                                                              <  1:1164109/131‑1 (MQ=255)                                                                           ttCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGTCAAAATATCCagag                                                     >  1:30865/1‑140 (MQ=255)                                                                                cTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGc                                                >  1:184435/1‑140 (MQ=255)                                                                                  aCGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGa                                              <  2:279470/140‑1 (MQ=255)                                                                                  aCGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGa                                              <  2:712776/140‑1 (MQ=255)                                                                                  aCGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGa                                              <  2:451033/140‑1 (MQ=255)                                                                                  aCGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAAAGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGa                                              >  2:111833/1‑140 (MQ=255)                                                                                                aaCTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACcgc                                >  1:533563/1‑140 (MQ=255)                                                                                                    ttCAGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAAt                            <  2:175257/140‑1 (MQ=255)                                                                                                       aGATCGTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAg                         <  2:30865/140‑1 (MQ=255)                                                                                                            gTCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAg                    >  2:82842/1‑140 (MQ=255)                                                                                                             tCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGc                   >  2:592874/1‑140 (MQ=255)                                                                                                             tCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGc                   >  2:645739/1‑140 (MQ=255)                                                                                                             tCAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGc                   >  2:659096/1‑140 (MQ=255)                                                                                                              cAATGTCAGCGCCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGca                  <  1:310790/140‑1 (MQ=255)                                                                                                                       cgcCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAgg            >  2:40722/1‑137 (MQ=255)                                                                                                                       cgcCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAgg            <  1:40722/137‑1 (MQ=255)                                                                                                                       cgcCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGa         <  1:82842/140‑1 (MQ=255)                                                                                                                       cgcCATCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGa         <  1:959809/140‑1 (MQ=255)                                                                                                                           aTCGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCt     >  1:753058/1‑140 (MQ=255)                                                                                                                             cGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTc   >  2:127619/1‑140 (MQ=255)                                                                                                                             cGTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTc   >  1:283206/1‑140 (MQ=255)                                                                                                                              gTTGTGCTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCt  <  1:645739/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                      ATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCT  >  NC_000913/3449926‑3450190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑