BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,453,557	G→A	A124V (GCC→GTC)	gspB ←	putative general secretion pathway protein B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,453,557	0	G	A	100.0%	378.7 / NA	107	A124V (GCC→GTC)	gspB	putative general secretion pathway protein B
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (54/53); total (54/53)

Only 100 of 108 total aligned reads displayed.

GCTGTCAACTACGACAAGCCCGCGCATTATACATACTTAACCACCGAACGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTG  >  NC_000913/3453423‑3453693

gctgTCAACTACGACAAGCCCGCGCATTATACATACTTAACCACCGAACGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTtc                                                                                                                                     >  1:412918/1‑140 (MQ=255)

 ctgTCAACTACGACAAGCCCGCGCATTATACATACTTAACCACCGAACGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTtct                                                                                                                                    >  1:94599/1‑140 (MQ=255)

      aaCTACGACAAGCCCGCGCATTATACATACTTAACCACCGAACGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCg                                                                                                                               <  1:845827/140‑1 (MQ=255)

      aaCTACGACAAGCCCGCGCATTATACATACTTAACCACCGAACGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCg                                                                                                                               <  1:364102/140‑1 (MQ=255)

        cTACGACAAGCCCGCGCATTATACATACTTAACCACCGAACGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCt                                                                                                                             >  1:815563/1‑140 (MQ=255)

           cGACAAGCCCGCGCATTATACATACTTAACCACCGAACGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTctct                                                                                                                                  >  1:787847/1‑132 (MQ=255)

           cGACAAGCCCGCGCATTATACATACTTAACCACCGAACGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTctct                                                                                                                                  <  2:787847/132‑1 (MQ=255)

           cGACAAGCCCGCGCATTATACATACTTAACCACCGAACGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTttc                                                                                                                          >  1:781820/1‑140 (MQ=255)

           cGACAAGCCCGCGCATTATACATACTTAACCACCGAACGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTttc                                                                                                                          >  1:25324/1‑140 (MQ=255)

               aaGCCCGCGCATTATACATACTTAACCACCGAACGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTcc                                                                                                                      <  2:113799/140‑1 (MQ=255)

                aGCCCGCGCATTATACATACTTAACCACCGAACGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCca                                                                                                                     <  1:352859/140‑1 (MQ=255)

                 gCCCGCGCATTATACATACTTAACCACCGAACGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCcac                                                                                                                    <  1:101724/140‑1 (MQ=255)

                                atacTTAACCACCGAACGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTCCGCTTTCTTCCACAGTATTTTCTTCGTc                                                                                                     >  1:439111/1‑140 (MQ=255)

                                 tacTTAACCACCGAACGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCt                                                                                                    >  2:751112/1‑140 (MQ=255)

                                  acTTAACCACCGAACGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCtt                                                                                                   <  2:351267/140‑1 (MQ=255)

                                   cTTAACCACCGAACGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTc                                                                                                  >  1:394122/1‑140 (MQ=255)

                                     tAACCACCGAACGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCag                                                                                                <  2:25324/140‑1 (MQ=255)

                                     tAACCACCGAACGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCag                                                                                                >  2:1171411/1‑140 (MQ=255)

                                     tAACCACCGAACGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCag                                                                                                >  1:626484/1‑140 (MQ=255)

                                     tAACCACCGAACGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCag                                                                                                >  1:537056/1‑140 (MQ=255)

                                      aaCCACCGAACGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTcgaa                                                                                               >  1:76618/1‑137 (MQ=255)

                                      aaCCACCGAACGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCaga                                                                                               >  1:1152968/1‑140 (MQ=255)

                                      aaCCACCGAACGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCaga                                                                                               >  1:50413/1‑140 (MQ=255)

                                       accaccGAACGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTttctt                                                                                                         <  2:454546/129‑1 (MQ=255)

                                       accaccGAACGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTttctt                                                                                                         >  1:454546/1‑129 (MQ=255)

                                       accaccGAACGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTc                                                                                                     >  2:842025/1‑133 (MQ=255)

                                       accaccGAACGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTc                                                                                                     <  1:842025/133‑1 (MQ=255)

                                       accaccGAACGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCtt                                                                                                   >  1:614487/1‑135 (MQ=255)

                                       accaccGAACGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCtt                                                                                                   <  2:614487/135‑1 (MQ=255)

                                       accaccGAACGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCagaa                                                                                              >  1:384430/1‑140 (MQ=255)

                                       accaccGAACGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCagaa                                                                                              >  2:1076901/1‑140 (MQ=255)

                                       accaccGAACGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCagaa                                                                                              >  1:641338/1‑140 (MQ=255)

                                       accaccGAACGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCagaa                                                                                              >  2:168607/1‑140 (MQ=255)

                                       accaccGAACGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCagaa                                                                                              >  2:729171/1‑140 (MQ=255)

                                           ccGAACGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCagaagaag                                                                                          <  1:49946/140‑1 (MQ=255)

                                            cGAACGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCagaagaaga                                                                                         <  2:893427/140‑1 (MQ=255)

                                            cGAACGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCagaagaaga                                                                                         >  1:172250/1‑140 (MQ=255)

                                             gAACGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAagag                                                                                        >  2:328161/1‑140 (MQ=255)

                                              aaCGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAgg                                                                                       >  1:371801/1‑140 (MQ=255)

                                              aaCGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAgg                                                                                       >  2:1159474/1‑140 (MQ=255)

                                                cGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAgggg                                                                                     <  1:729171/140‑1 (MQ=255)

                                                cGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAgggg                                                                                     <  2:1022621/140‑1 (MQ=255)

                                                cGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAgggg                                                                                     <  2:448037/140‑1 (MQ=255)

                                                cGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAgggg                                                                                     <  1:781767/140‑1 (MQ=255)

                                                cGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAgggg                                                                                     <  1:751112/140‑1 (MQ=255)

                                                cGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAgggg                                                                                     <  1:328161/140‑1 (MQ=255)

                                                cGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAgggg                                                                                     <  2:537056/140‑1 (MQ=255)

                                                     gCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCAtt                                                                                <  2:394122/140‑1 (MQ=255)

                                                        tATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATtctc                                                                             <  1:168607/140‑1 (MQ=255)

                                                        tATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATtctc                                                                             >  1:125093/1‑140 (MQ=255)

                                                          ttGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCaa                                                                           >  1:210417/1‑140 (MQ=255)

                                                             cTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCagaagaag                                                                                          >  2:43486/1‑122 (MQ=255)

                                                              tAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACcgc                                                                       <  2:844942/140‑1 (MQ=255)

                                                              tAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTCCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACcgc                                                                       <  2:439111/140‑1 (MQ=255)

                                                                  aaagtaaaCAGCCTTCGGGTTATCTCTCAAGTTCGTTAAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTGTTCGTCTGCAGAAGAAGAGGGGGCATTCTCAACCTCGACg                                                                   >  2:1034414/1‑140 (MQ=255)

                                                                    agtaaaCAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACcgc                                                                       >  1:155077/1‑134 (MQ=255)

                                                                    agtaaaCAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACcgc                                                                       <  2:155077/134‑1 (MQ=255)

                                                                    agtaaaCAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGcc                                                                 <  1:980505/140‑1 (MQ=255)

                                                                    agtaaaCAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGcc                                                                 <  1:1124834/140‑1 (MQ=255)

                                                                     gtaaaCAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCt                                                                <  1:1076901/140‑1 (MQ=255)

                                                                      taaaCAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTg                                                               <  1:936877/140‑1 (MQ=255)

                                                                        aaCAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTggg                                                             >  1:1038815/1‑140 (MQ=255)

                                                                        aaCAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTggg                                                             <  2:50413/140‑1 (MQ=255)

                                                                        aaCAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACG‑‑CTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGt                                                            <  1:317286/139‑1 (MQ=255)

                                                                        aaCAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACG‑‑CTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGt                                                            >  2:317286/1‑139 (MQ=255)

                                                                         aCAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGt                                                            <  2:210417/140‑1 (MQ=255)

                                                                          cAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTc                                                           <  2:641338/140‑1 (MQ=255)

                                                                             ccTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCt                                                                >  1:667606/1‑132 (MQ=255)

                                                                               ttCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCtt                                                      >  2:566035/1‑140 (MQ=255)

                                                                                tCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTtc                                                     >  1:220629/1‑140 (MQ=255)

                                                                                       aTCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCg                                              >  1:659323/1‑140 (MQ=255)

                                                                                       aTCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCg                                              <  2:309614/140‑1 (MQ=255)

                                                                                       aTCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCg                                              >  2:640876/1‑140 (MQ=255)

                                                                                        tctctcAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGa                                             <  2:220629/140‑1 (MQ=255)

                                                                                          tctcAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACg                                           <  2:815563/140‑1 (MQ=255)

                                                                                            tcAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGa                                         <  1:932443/140‑1 (MQ=255)

                                                                                              aaGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCg                                              <  2:934129/133‑1 (MQ=255)

                                                                                              aaGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCg                                              >  1:934129/1‑133 (MQ=255)

                                                                                              aaGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGAtt                                       <  1:123424/140‑1 (MQ=255)

                                                                                                 ttcgttcAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTcga                                    <  2:483294/140‑1 (MQ=255)

                                                                                                  tcgttcAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTcgac                                   >  2:66647/1‑140 (MQ=255)

                                                                                                   cgttcAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTcgacg                                  >  2:643689/1‑140 (MQ=255)

                                                                                                   cgttcAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTcgacg                                  >  2:823496/1‑140 (MQ=255)

                                                                                                   cgttcAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTCGGTCATCTTCCTCCTCGACGGATTcgacg                                  >  1:692828/1‑140 (MQ=255)

                                                                                                      tcAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGacgacg                               <  1:1034414/140‑1 (MQ=255)

                                                                                                      tcAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGacgacg                               <  1:823496/140‑1 (MQ=255)

                                                                                                      tcAGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGacgacg                               >  2:783515/1‑140 (MQ=255)

                                                                                                        aGTGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACggg                             <  2:1152968/140‑1 (MQ=255)

                                                                                                          tGCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGa                           >  1:175891/1‑140 (MQ=255)

                                                                                                           gCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGacgacg                               <  1:72163/135‑1 (MQ=255)

                                                                                                           gCATTTTTGACACGCTCTCTTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGacgacg                               >  2:72163/1‑135 (MQ=255)

                                                                                                                tttGACACGCTCTCTTAATCAGACTTTCTCTTCGCTTTCTGCCGCAGTATTTTCTTCGTCCTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGAAGGATTCGACGACGGGGAGTGGCt                     <  2:692828/140‑1 (MQ=255)

                                                                                                                         ctctctTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTga            >  1:59951/1‑140 (MQ=255)

                                                                                                                         ctctctTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTga            >  1:276842/1‑140 (MQ=255)

                                                                                                                         ctctctTAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTga            >  1:735643/1‑140 (MQ=255)

                                                                                                                              ttAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGaaa       <  2:371801/140‑1 (MQ=255)

                                                                                                                              ttAAACCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGaaa       <  1:1159474/140‑1 (MQ=255)

                                                                                                                                aaaCCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGAAATa     <  1:566035/140‑1 (MQ=255)

                                                                                                                                aaaCCGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGAAATa     <  1:783515/140‑1 (MQ=255)

                                                                                                                                   ccGACTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAgtg  <  2:179200/140‑1 (MQ=255)

GCTGTCAACTACGACAAGCCCGCGCATTATACATACTTAACCACCGAACGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTG  >  NC_000913/3453423‑3453693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`