Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,561,338	G→A	L39L (CTG→TTG)	glpG ←	rhomboid protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,561,338	0	G	A	100.0%	263.3 / NA	74	L39L (CTG→TTG)	glpG	rhomboid protease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/42);  total (32/42)

TAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGTTAGCAAAAGAGGTAATCATCAACATCGTTGCTGTCGCTTATTCCA  >  NC_000913/3561205‑3561472                                                                                                                                      |                                                                                                                                       tAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGAc                                                                                                                                  <  2:276571/140‑1 (MQ=255) tAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGAc                                                                                                                                  <  1:809960/140‑1 (MQ=255)  aaCGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACa                                                                                                                                 >  1:519031/1‑140 (MQ=255)   aCGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACAt                                                                                                                                <  1:945420/140‑1 (MQ=255)    cGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAAACATc                                                                                                                               >  1:25280/1‑140 (MQ=255)      gCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGc                                                                                                                             <  1:718932/140‑1 (MQ=255)      gCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGc                                                                                                                             <  1:1101112/140‑1 (MQ=255)      gCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGc                                                                                                                             <  2:817939/140‑1 (MQ=255)         aTAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGc                                                                                                                             >  1:589804/1‑137 (MQ=255)         aTAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGc                                                                                                                             <  2:589804/137‑1 (MQ=255)           aaTGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTgg                                                                                                                        <  1:144585/140‑1 (MQ=255)              gCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTa                                                                                                                     <  2:237909/140‑1 (MQ=255)              gCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTa                                                                                                                     <  1:824846/140‑1 (MQ=255)              gCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTa                                                                                                                     >  1:189773/1‑140 (MQ=255)                  gCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTGTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTAtgtt                                                                                                                 >  2:807525/1‑140 (MQ=255)                     aCTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATgttgtt                                                                                                              <  1:661522/140‑1 (MQ=255)                      cTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCt                                                                                                                            >  2:1149207/1‑125 (MQ=255)                      cTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCt                                                                                                                            <  1:1149207/125‑1 (MQ=255)                            gTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGCCTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGt                                                                                                       <  2:804473/140‑1 (MQ=255)                            gTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGt                                                                                                       <  1:208888/140‑1 (MQ=255)                            gTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGt                                                                                                       <  2:519031/140‑1 (MQ=255)                              aTGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGa                                                                                                     <  2:184826/140‑1 (MQ=255)                                ggCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAgg                                                                                                   >  1:1031324/1‑140 (MQ=255)                                 gcctgcctGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGa                                                                                                  >  2:334120/1‑140 (MQ=255)                                  cctgcctgCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGAt                                                                                                 >  2:296580/1‑140 (MQ=255)                                  cctgcctgCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGAt                                                                                                 <  1:61981/140‑1 (MQ=255)                                   ctgcctgcCAGCTCGCCGCCAGATAATGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATa                                                                                                >  1:1102647/1‑140 (MQ=255)                                      cctgccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGa                                                                                                            >  1:123014/1‑125 (MQ=255)                                      cctgccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGa                                                                                                            <  2:123014/125‑1 (MQ=255)                                      cctgccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAaca                                                                                             >  1:1029556/1‑140 (MQ=255)                                      cctgccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAaca                                                                                             >  2:232975/1‑140 (MQ=255)                                      cctgccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAaca                                                                                             >  2:663384/1‑140 (MQ=255)                                      cctgccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAaca                                                                                             >  1:189795/1‑140 (MQ=255)                                       ctgccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAacac                                                                                            >  2:312773/1‑140 (MQ=255)                                          ccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCt                                                                                         <  2:1029556/140‑1 (MQ=255)                                           cAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTg                                                                                        >  1:941078/1‑140 (MQ=255)                                              cTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGt                                                                                     <  2:956180/140‑1 (MQ=255)                                               tCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTc                                                                                    <  2:453561/140‑1 (MQ=255)                                               tCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTc                                                                                    <  1:371169/140‑1 (MQ=255)                                                     ccAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATg                                                                              <  2:941078/140‑1 (MQ=255)                                                        gATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTaa                                                                           >  2:899525/1‑140 (MQ=255)                                                        gATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTaa                                                                           >  2:602797/1‑140 (MQ=255)                                                             cgcgGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATcaac                                                                      >  1:932284/1‑140 (MQ=255)                                                                 gATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAAc                                                                  >  2:151982/1‑140 (MQ=255)                                                                      gCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTg                                                             >  2:907938/1‑140 (MQ=255)                                                                       ccGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTgc                                                            >  1:545824/1‑140 (MQ=255)                                                                       ccGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTgc                                                            >  2:273422/1‑140 (MQ=255)                                                                        cGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTgcg                                                           <  1:93448/140‑1 (MQ=255)                                                                               tCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCAcgcg                                                    <  1:232975/140‑1 (MQ=255)                                                                                  agaAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCgggg                                                 <  2:787778/140‑1 (MQ=255)                                                                                     aaaCGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGtt                                              <  2:545824/140‑1 (MQ=255)                                                                                     aaaCGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGtt                                              <  1:907938/140‑1 (MQ=255)                                                                                      aaCGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCAcg                                                      >  2:298619/1‑131 (MQ=255)                                                                                      aaCGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCAcg                                                      <  1:298619/131‑1 (MQ=255)                                                                                        cgcgCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGTTAGc                                           >  1:822266/1‑140 (MQ=255)                                                                                          cgcCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGTTAGCaa                                         <  1:312773/140‑1 (MQ=255)                                                                                           gcCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGTTAGCaaa                                        <  1:273422/140‑1 (MQ=255)                                                                                              aGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGTTAGCAAAaga                                     <  1:785466/140‑1 (MQ=255)                                                                                                cTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGTTAGCAAAAGAgg                                   <  2:932284/140‑1 (MQ=255)                                                                                                    gCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCTTGCGCCACGCGGGGGTTAGCAAAAGAGGTAat                               <  2:975224/140‑1 (MQ=255)                                                                                                     cccGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGTTAGCAAAAGAGGTAatc                              >  2:774020/1‑140 (MQ=255)                                                                                                       cGTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGTTAGCAAAAGAGGTAatcat                            <  1:774020/140‑1 (MQ=255)                                                                                                        gTACGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGTTAGCAAAAGAGGTAatcatc                           <  1:663384/140‑1 (MQ=255)                                                                                                          aCGCGCTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGTTAGCAAAAGAGGTAATCATCaa                         >  2:572099/1‑140 (MQ=255)                                                                                                               cTCGGCCTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGTTAGCAAAAGAGGTAATCATCAACATCg                    <  1:602797/140‑1 (MQ=255)                                                                                                                    ccTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGTTAGCAAAAGAGGTAATCATCAACATCGTTGCt               <  1:572099/140‑1 (MQ=255)                                                                                                                    ccTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGTTAGCAAAAGAGGTAATCATCAACATCGTTGCt               <  1:967946/140‑1 (MQ=255)                                                                                                                     cTGGGACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGTTAGCAAAAGAGGTAATCATCAACATCGTTGCTg              <  1:334120/140‑1 (MQ=255)                                                                                                                        ggACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGTTAGCAAAAGAGGTAATCATCAACATCGTTGCTGTCg           >  2:642000/1‑140 (MQ=255)                                                                                                                        agACTCATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGTTAGCAAAAGAGGTAATCATCAACATCGTTGCTGTCg           >  2:519839/2‑140 (MQ=255)                                                                                                                             cATCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGTTAGCAAAAGAGGTAATCATCAACATCGTTGCTGTCGCTTAt      >  1:291470/1‑140 (MQ=255)                                                                                                                              aTCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGTTAGCAAAAGAGGTAATCATCAACATCGTTGCTGTCGc          >  1:397780/1‑135 (MQ=255)                                                                                                                              aTCCGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGTTAGCAAAAGAGGTAATCATCAACATCGTTGCTGTCGc          <  2:397780/135‑1 (MQ=255)                                                                                                                                 cGCCAACCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGTTAGCAAAAGAGGTAATCATCAACATCGTTGCTGTCGCTTATTCca  <  1:182663/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       TAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGTTAGCAAAAGAGGTAATCATCAACATCGTTGCTGTCGCTTATTCCA  >  NC_000913/3561205‑3561472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑