Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,830,613	G→A	D53N (GAT→AAT)	yicH →	AsmA famly protein YicH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,830,613	0	G	A	100.0%	259.7 / NA	73	D53N (GAT→AAT)	yicH	AsmA famly protein YicH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/38);  total (35/38)

CATTCTCATCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAGCAGAACATATCAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGGATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGCCAGCCCGCGACCCTGGTGGCCAAAAGTGTCGACATTGCGCTAAGCAGTCGGCAACTGACCGAACCACGCCATGT  >  NC_000913/3830486‑3830749                                                                                                                                |                                                                                                                                         cATTCTCATCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAGCAGAACATATCAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGttttt                                                                                                                              >  1:286991/1‑140 (MQ=255)      tcATCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAGCAGAACATATCAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCcgcg                                                                                                                         <  1:569596/140‑1 (MQ=255)      tcATCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAGCAGAACATATCAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCcgcg                                                                                                                         <  2:1014292/140‑1 (MQ=255)      tcATCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAGCAGAACATATCAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCcgcg                                                                                                                         <  1:328565/140‑1 (MQ=255)      tcATCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAGCAGAACATATCAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCcgcg                                                                                                                         >  1:1114087/1‑140 (MQ=255)      tcATCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAGCAGAACATATCAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCcgcg                                                                                                                         <  2:474978/140‑1 (MQ=255)      tcATCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAGCAGAACATATCAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCcgcg                                                                                                                         <  2:924907/140‑1 (MQ=255)      tcATCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAGCAGAACATATCAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCcgcg                                                                                                                         <  1:1034799/140‑1 (MQ=255)      tcATCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAGCAGAACATATCAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCcgcg                                                                                                                         <  1:443161/140‑1 (MQ=255)       cATCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAGCAGAACATATCAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCcgcg                                                                                                                         >  1:1046766/1‑139 (MQ=255)       cATCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAGCAGAACATATCAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCcgcg                                                                                                                         <  2:1046766/139‑1 (MQ=255)       cATCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAGCAGAACATATCAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCCgcgc                                                                                                                        >  2:346460/1‑140 (MQ=255)        aTCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAGCAGAACATATCAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCCGCGcc                                                                                                                       <  1:1034278/140‑1 (MQ=255)            ctctGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAGCAGAACATATCAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCcg                                                                                                                           >  2:280413/1‑132 (MQ=255)            ctctGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAGCAGAACATATCAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCcg                                                                                                                           <  1:280413/132‑1 (MQ=255)               tGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAGCAGAACATATCAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCcgcg                                                                                                                         >  2:848548/1‑131 (MQ=255)               tGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAGCAGAACATATCAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCcgcg                                                                                                                         <  1:848548/131‑1 (MQ=255)                 ttAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAGCAGAACATATCAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCatat                                                                                                              <  2:379178/140‑1 (MQ=255)                  tAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAGCAGAACATATCAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATc                                                                                                             <  2:842697/140‑1 (MQ=255)                     tGGCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAGCAGAACATATCAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTg                                                                                                          <  2:97129/140‑1 (MQ=255)                      ggCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAGCAGAACATATCAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCcgcg                                                                                                                         >  2:375103/1‑124 (MQ=255)                      ggCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAGCAGAACATATCAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCcgcg                                                                                                                         <  1:375103/124‑1 (MQ=255)                      ggCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAGCAGAACATATCAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGc                                                                                                         >  2:105715/1‑140 (MQ=255)                         gATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAGCAGAACATATCAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCACATCGTGCTgg                                                                                                      >  1:1031728/1‑140 (MQ=255)                          aTCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAGCAGAACATATCAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGga                                                                                                     >  1:913618/1‑140 (MQ=255)                           tCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAGCAGAACATATCAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGgag                                                                                                    >  1:136413/1‑140 (MQ=255)                           tCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAGCAGAACATATCAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCCTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGgag                                                                                                    >  2:1051128/1‑140 (MQ=255)                                            cttctGCAAACCCGCTGGGGAGCAGAACATATCAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCg                                                                                   >  1:1056241/1‑140 (MQ=255)                                              tctGCAAACCCGCTGGGGAGCAGAACATATCAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTg                                                                                 <  2:136413/140‑1 (MQ=255)                                                tGCAAACCCGCTGGGGAGCAGAACATATCAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGAt                                                                               >  2:268997/1‑140 (MQ=255)                                                tGCAAACCCGCTGGGGAGCAGAACATATCAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCAGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGAt                                                                               >  2:813140/1‑140 (MQ=255)                                                    aaCCCGCTGGGGAGCAGAACATATCAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGcc                                                                           <  2:252214/140‑1 (MQ=255)                                                     aCCCGCTGGGGAGCAGAACATATCAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGCCa                                                                          <  2:1114087/140‑1 (MQ=255)                                                          cTGGGGAGCAGAACATATCAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGCCAGCCcg                                                                     >  2:232533/1‑140 (MQ=255)                                                                  cAGAACATATCAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGCCAGCCCGCGACCCtg                                                             <  1:706139/140‑1 (MQ=255)                                                                  cAGAACATATCAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGCCAGCCCGCGACCCtg                                                             <  1:105715/140‑1 (MQ=255)                                                                  cAGAACATATCAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCACATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGCCAGCCCGCGACCCtg                                                             <  2:1031728/140‑1 (MQ=255)                                                                         tatCAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGCCAGCCCGCGACCCTGGTGGCCa                                                      >  1:1084775/1‑140 (MQ=255)                                                                         tatCAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGCCAGCCCGCGACCCTGGTGGCCa                                                      <  1:108391/140‑1 (MQ=255)                                                                          atCAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGCCAGCCCGCGACCCTGGTGGCCaa                                                     >  1:112232/1‑140 (MQ=255)                                                                          atCAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGCCAGCCCGCGACCCTGGTGGCCaa                                                     >  1:32908/1‑140 (MQ=255)                                                                           tCAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGCCAGCCCGCGACCCTGGTGGCCaaa                                                    <  2:1056241/140‑1 (MQ=255)                                                                            cAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGCCAGCCCGCGACCCTGGTGGCCaaaa                                                   >  1:1024337/1‑140 (MQ=255)                                                                               cgcATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGCCAGCCCGCGACCCTGGTGGCCa                                                      >  1:557135/1‑134 (MQ=255)                                                                               cgcATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGCCAGCCCGCGACCCTGGTGGCCa                                                      <  2:557135/134‑1 (MQ=255)                                                                               cgcATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGCCAGCCCGCGACCCTGGTGGCCAAAAgtg                                                >  2:679136/1‑140 (MQ=255)                                                                               cgcATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGCCAGCCCGCGACCCTGGTGGCCAAAAgtg                                                >  1:83672/1‑140 (MQ=255)                                                                                 cATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGCCAGCCCGCGACCCTGGTGGCCAAAAGTGTc                                              >  1:91842/1‑140 (MQ=255)                                                                                     ggTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGCCAGCCCGCGACCCTGGTGGCCAAAAGTGTCGACa                                          <  1:679136/140‑1 (MQ=255)                                                                                     ggTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGCCAGCCCGCGACCCTGGTGGCCAAAAGTGTCGACa                                          <  2:112232/140‑1 (MQ=255)                                                                                     ggTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGCCAGCCCGCGACCCTGGTGGCCAAAAGTGTCGACa                                          <  1:268997/140‑1 (MQ=255)                                                                                         tCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGCCAGCCCGCGACCCTGGTGGCCAAAAGTGTCGACATTgc                                      >  1:548252/1‑140 (MQ=255)                                                                                               aaTAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGCCAGCCCGCGACCCTGGTGGCCAAAAGTGTCGACATTGCGCTAAg                                >  1:104619/1‑140 (MQ=255)                                                                                               aaTAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGCCAGCCCGCGACCCTGGTGGCCAAAAGTGTCGACATTGCGCTAAg                                >  1:105495/1‑140 (MQ=255)                                                                                               aaTAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGCCAGCCCGCGACCCTGGTGGCCAAAAGTGTCGACATTGCGCTAAg                                <  1:1051128/140‑1 (MQ=255)                                                                                                 tAGCGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGCCAGCCCGCGACCCTGGTGGCCAAAAGTGTCGACATTGCGCTAAGCa                              >  2:734795/1‑140 (MQ=255)                                                                                                    cGACTATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGCCAGCCCGCGACCCTGGTGGCCAAAAGTGTCGACATTGCGCTAAGCAGTc                           >  2:941808/1‑140 (MQ=255)                                                                                                        tATCATCTGGCCTTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGCCAGCCCGCGACCCTGGTGGCCAAAAGTGTCGACATTGCGCTAAGCAGTCGGCa                       >  2:1085287/1‑140 (MQ=255)                                                                                                         atcatcTGGCCTTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGCCAGCCCGCGACCCTGGTGGCCAAAAGTGTCGACATTGCGCTAAGCAGTCGGCaa                      >  1:177112/1‑140 (MQ=255)                                                                                                            atcTGGCCTTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGCCAGCCCGCGACCCTGGTGGCCAAAAGTGTCGACATTGCGCTAAGCAGTCGGCAACTg                   >  2:738080/1‑140 (MQ=255)                                                                                                              cTGGCCTTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGCCAGCCCGCGACCCTGGTGGCCAAAAGTGTCGACATTGCGCTAAGCAGTCGGCAACTGAc                 >  2:416448/1‑140 (MQ=255)                                                                                                              cTGGCCTTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGCCAGCCCGCGACCCTGGTGGCCAAAAGTGTCGACATTGCGCTAAGCAGTCGGCAACTGAc                 <  2:32908/140‑1 (MQ=255)                                                                                                              cTGGCCTTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGCCAGCCCGCGACCCTGGTGGCCAAAAGTGTCGACATTGCGCTAAGCAGTCGGCAACTGAc                 <  1:232533/140‑1 (MQ=255)                                                                                                               tGGCCTTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGCCAGCCCGCGACCCTGGTGGCCAAAAGTGTCGACATTGCGCTAACCAGTCGGCAACTGAcc                <  2:913618/140‑1 (MQ=255)                                                                                                                  ccTTCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGCCAGCCCGCGACCCTGGTGGCCAAAAGTGTCGACATTGCGCTAAGCAGTCGGCAACTGACCGaa             <  1:346460/140‑1 (MQ=255)                                                                                                                    ttCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGCCAGCCCGCGACCCTGGTGGCCAAAAGTGTCGACATTGCGCTAAGCAGTCGGCa                       >  1:433837/1‑128 (MQ=255)                                                                                                                    ttCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGCCAGCCCGCGACCCTGGTGGCCAAAAGTGTCGACATTGCGCTAAGCAGTCGGCa                       <  2:433837/128‑1 (MQ=255)                                                                                                                     tCGGGGCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGCCAGCCCGCGACCCTGGTGGCCAAAAGTGTCGACATTGCGCTAAGCAGTCGGCAACTGACCGAACCa          >  2:1081069/1‑140 (MQ=255)                                                                                                                       ggggCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGCCAGCCCGCGACCCTGGTGGCCAAAAGTGTCGACATTGCGCTAAGCAGTCGGCAACTGACCGAACCACg        <  1:738080/140‑1 (MQ=255)                                                                                                                         ggCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGCCAGCCCGCGACCCTGGTGGCCAAAAGTGTCGACATTGCGCTAAGCAGTCGGCAACTGACCGAACCACGcc      <  2:83672/140‑1 (MQ=255)                                                                                                                         ggCGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGCCAGCCCGCGACCCTGGTGGCCAAAAGTGTCGACATTGCGCTAAGCAGTCGGCAACTGACCGAACCACGcc      <  2:286991/140‑1 (MQ=255)                                                                                                                           cGATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGCCAGCCCGCGACCCTGGTGGCCAAAAGTGTCGACATTGCGCTAAGCAGTCGGCAACTGACCGAACCACGCCAt    <  2:548252/140‑1 (MQ=255)                                                                                                                            gATGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGCCAGCCCGCGACCCTGGTGGCCAAAAGTGTCGACATTGCGCTAAGCAGTCGGCAACTGACCGAACCACGCCATg   >  2:526223/1‑140 (MQ=255)                                                                                                                             aTGAATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGCCAGCCCGCGACCCTGGTGGCCAAAAGTGTCGACATTGCGCTAAGCAGTCGGCAACTGACCGAACCACGCCATGt  <  2:104619/140‑1 (MQ=255)                                                                                                                                |                                                                                                                                         CATTCTCATCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAGCAGAACATATCAGCGCATGGGTTTCCGAGAATAGCGACTATCATCTGGCCTTCGGGGCGATGGATCACCGTTTTTCCGCGCCATCTCATATCGTGCTGGAGAACGTCACGTTTGGTCGTGATGGCCAGCCCGCGACCCTGGTGGCCAAAAGTGTCGACATTGCGCTAAGCAGTCGGCAACTGACCGAACCACGCCATGT  >  NC_000913/3830486‑3830749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑