Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,052,658	C→T	S205F (TCC→TTC)	hemN →	coproporphyrinogen III dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,052,658	0	C	T	100.0%	283.9 / NA	79	S205F (TCC→TTC)	hemN	coproporphyrinogen III dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (39/40);  total (39/40)

CGCGCCGAAGGCTTTAATCGCCTGAGCATGGGCGTGCAGGACTTCAACAAAGAAGTGCAACGTCTGGTTAACCGCGAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTCCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCAT  >  NC_000913/4052522‑4052787                                                                                                                                         |                                                                                                                                  cgcgCCGAAGGCTTTAATCGCCTGAGCATGGGCGTGCAGGACTTCAACAAAGAAGTGCAACGTCTGGTTAACCGCGAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTtcac                                                                                                                                <  2:759711/140‑1 (MQ=255)     ccGAAGGCTTTAATCGCCTGAGCATGGGCGTGCAGGACTTCAACAAAGAAGTGCAACGTCTGGTTAACCGCGAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAAc                                                                                                                            >  1:642175/1‑140 (MQ=255)      cGAAGGCTTTAATCGCCTGAGCATGGGCGTGCAGGACTTCAACAAAGAAGTGCAACGTCTGGTTAACCGCGAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACa                                                                                                                           <  1:1080078/140‑1 (MQ=255)      cGAAGGCTTTAATCGCCTGAGCATGGGCGTGCAGGACTTCAACAAAGAAGTGCAACGTCTGGTTAACCGCGAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACa                                                                                                                           >  1:479683/1‑140 (MQ=255)      cGAAGGCTTTAATCGCCTGAGCATGGGCGTGCAGGACTTCAACAAAGAAGTGCAACGTCTGGTTAACCGCGAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACa                                                                                                                           <  2:642175/140‑1 (MQ=255)       gAAGGCTTTAATCGCCTGAGCATGGGCGTGCAGGACTTCAACAAAGAAGTGCAACGTCTGGTTAACCGCGAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACAt                                                                                                                          <  2:954458/140‑1 (MQ=255)        aaGGCTTTAATCGCCTGAGCATGGGCGTGCAGGACTTCAACAAAGAAGTGCAACGTCTGGTTAACCGCGAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATc                                                                                                                         >  1:539805/1‑140 (MQ=255)        aaGGCTTTAATCGCCTGAGCATGGGCGTGCAGGACTTCAACAAAGAAGTGCAACGTCTGGTTAACCGCGAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATc                                                                                                                         <  1:220563/140‑1 (MQ=255)                     ccTGAGCATGGGCGTGCAGGACTTCAACAAAGAAGTGCAACGTCTGGTTAACCGCGAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACg                                                                                                            >  2:1140653/1‑140 (MQ=255)                       tGAGCATGGGCGTGCAGGACTTCAACAAAGAAGTGCAACGTCTGGTTAACCGCGAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGc                                                                                                          >  1:266527/1‑140 (MQ=255)                          gCATGGGCGTGCAGGACTTCAACAAAGAAGTGCAACGTCTGGTTAACCGCGAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTg                                                                                                       <  1:885783/140‑1 (MQ=255)                               ggCGTGCAGGACTTCAACAAAGAAGTGCAACGTCTGGTTAACCGCGAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGaa                                                                                                  <  2:434439/140‑1 (MQ=255)                                gCGTGCAGGACTTCAACAAAGAAGTGCAACGTCTGGTTAACCGCGAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGaaa                                                                                                 >  2:380441/1‑140 (MQ=255)                                   tGCAGGACTTCAACAAAGAAGTGCAACGTCTGGTTAACCGCGAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAg                                                                                              >  1:86606/1‑140 (MQ=255)                                   tGCAGGACTTCAACAAAGAAGTGCAACGTCTGGTTAACCGCGAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAg                                                                                              >  1:656566/1‑140 (MQ=255)                                   tGCAGGACTTCAACAAAGAAGTGCAACGTCTGGTTAACCGCGAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAg                                                                                              >  1:1171969/1‑140 (MQ=255)                                   tGCAGGACTTCAACAAAGAAGTGCAACGTCTGGTTAACCGCGAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAg                                                                                              <  1:380115/140‑1 (MQ=255)                                    gCAGGACTTCAACAAAGAAGTGCAACGTCTGGTTAACCGCGAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGa                                                                                             <  1:754083/140‑1 (MQ=255)                                          cTTCAACAAAGAAGTGCAACGTCTGGTTAACCGCGAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCgg                                                                                       >  1:380233/1‑140 (MQ=255)                                          cTTCAACAAAGAAGTGCAACGTCTGGTTAACCGCGAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCgg                                                                                       >  2:411333/1‑140 (MQ=255)                                             caacaaAGAAGTGCAACGTCTGGTTAACCGCGAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGgaga                                                                                    >  1:978497/1‑140 (MQ=255)                                               acaaAGAAGTGCAACGTCTGGTTAACCGCGAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGt                                                                                  <  2:301805/140‑1 (MQ=255)                                                 aaaGAAGTGCAACGTCTGGTTAACCGCGAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGttt                                                                                <  1:1140653/140‑1 (MQ=255)                                                  aagaagTGCAACGTCTGGTTAACCGCGAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTc                                                                               >  1:262817/1‑140 (MQ=255)                                                     aagTGCAACGTCTGGTTAACCGCGAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGcc                                                                            >  2:648485/1‑140 (MQ=255)                                                      agTGCAACGTCTGGTTAACCGCGAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCt                                                                           <  2:656566/140‑1 (MQ=255)                                                      agTGCAACGTCTGGTTAACCGCGAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCt                                                                           <  1:174227/140‑1 (MQ=255)                                                      agTGCAACGTCTGGTTAACCGCGAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCt                                                                           <  2:86606/140‑1 (MQ=255)                                                      agTGCAACGTCTGGTTAACCGCGAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCt                                                                           <  1:645237/140‑1 (MQ=255)                                                      agTGCAACGTCTGGTTAACCGCGAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCt                                                                           <  1:648485/140‑1 (MQ=255)                                                        tGCAACGTCTGGTTAACCGCGAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCttt                                                                         >  2:130829/1‑140 (MQ=255)                                                        tGCAACGTCTGGTTAACCGCGAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCttt                                                                         <  2:874321/140‑1 (MQ=255)                                                        tGCAACGTCTGGTTAACCGCGAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCttt                                                                         >  1:370112/1‑140 (MQ=255)                                                        tGCAACGTCTGGTTAACCGCGAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTAATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCttt                                                                         >  2:1091520/1‑140 (MQ=255)                                                               tCTGGTTAACCGCGAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGa                                                                  >  1:40169/1‑140 (MQ=255)                                                               tCTGGTTAACCGCGAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGa                                                                  >  2:939621/1‑140 (MQ=255)                                                               tCTGGTTAACCGCCAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCtt                                                                          <  1:930021/132‑1 (MQ=255)                                                               tCTGGTTAACCGCCAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCtt                                                                          >  2:930021/1‑132 (MQ=255)                                                                cTGGTTAACCGCGAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGaa                                                                 <  2:978497/140‑1 (MQ=255)                                                                cTGGTTAACCGCGAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGaa                                                                 <  2:266527/140‑1 (MQ=255)                                                                  ggTTAACCGCGAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAAc                                                               >  1:682312/1‑140 (MQ=255)                                                                         cgcgAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGc                                                        >  2:515979/1‑140 (MQ=255)                                                                         cgcgAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGc                                                        >  2:165042/1‑140 (MQ=255)                                                                             aGCAGGATGAAGGGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAg                                                    >  1:406635/1‑140 (MQ=255)                                                                             aGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAGACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAg                                                    >  1:198774/1‑140 (MQ=255)                                                                               cAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCt                                                  <  2:682312/140‑1 (MQ=255)                                                                                aGGATGAAGGGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTg                                                 <  2:406635/140‑1 (MQ=255)                                                                                    tGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAAcc                                             <  2:423353/140‑1 (MQ=255)                                                                                      aaGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAAcccc                                           <  1:515979/140‑1 (MQ=255)                                                                                      aaGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAAcccc                                           <  1:1289/140‑1 (MQ=255)                                                                                      aaGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAAcccc                                           <  2:40169/140‑1 (MQ=255)                                                                                       agagTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCg                                          <  1:366857/140‑1 (MQ=255)                                                                                        gagTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGa                                         >  1:534148/1‑140 (MQ=255)                                                                                        gagTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGa                                         <  1:380441/140‑1 (MQ=255)                                                                                         agTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAGACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGAt                                        <  2:198774/140‑1 (MQ=255)                                                                                         agTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGAt                                        <  2:534148/140‑1 (MQ=255)                                                                                                cTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGa                                 <  2:262817/140‑1 (MQ=255)                                                                                                cTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGa                                 <  2:479683/140‑1 (MQ=255)                                                                                                cTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGa                                 <  1:130829/140‑1 (MQ=255)                                                                                                  ttGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAgt                               <  1:1091520/140‑1 (MQ=255)                                                                                                   tGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAgtg                              >  2:432436/1‑140 (MQ=255)                                                                                                        tGCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCttt                         >  2:802045/1‑140 (MQ=255)                                                                                                         gCTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTa                        >  1:182667/1‑140 (MQ=255)                                                                                                          cTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATc                                       <  2:1167113/124‑1 (MQ=255)                                                                                                          cTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATc                                       >  1:1167113/1‑124 (MQ=255)                                                                                                          cTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGa                                 >  2:496909/1‑130 (MQ=255)                                                                                                          cTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGa                                 <  1:496909/130‑1 (MQ=255)                                                                                                          cTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTaa                       <  1:411333/140‑1 (MQ=255)                                                                                                          cTTAACCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTaa                       <  1:165042/140‑1 (MQ=255)                                                                                                             aaCCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAgt                               >  2:954979/1‑129 (MQ=255)                                                                                                             aaCCATGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAgt                               <  1:954979/129‑1 (MQ=255)                                                                                                                  tGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACgcgc               >  1:902487/1‑140 (MQ=255)                                                                                                                  tGCGCGTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACgcgc               >  2:955747/1‑140 (MQ=255)                                                                                                                    cgcgTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCAt             >  2:579889/1‑140 (MQ=255)                                                                                                                    cgcgTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCAt             >  1:392688/1‑140 (MQ=255)                                                                                                                    cgcgTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCAt             >  2:1045011/1‑140 (MQ=255)                                                                                                                    cgcgTGAGATTGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCAt             >  1:686144/1‑140 (MQ=255)                                                                                                                    cgcgTGAGATAGGTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCAt             <  1:802045/140‑1 (MQ=255)                                                                                                                         gagaTTGGTTTTACCTTCACCAACATCGACCTGA‑TTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCg       >  2:53614/1‑140 (MQ=255)                                                                                                                               ggTTTTACCTTCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCAt  <  1:1045011/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                  CGCGCCGAAGGCTTTAATCGCCTGAGCATGGGCGTGCAGGACTTCAACAAAGAAGTGCAACGTCTGGTTAACCGCGAGCAGGATGAAGAGTTCATCTTTGCACTGCTTAACCATGCGCGTGAGATTGGTTTTACCTCCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCAT  >  NC_000913/4052522‑4052787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑