Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,242,009	G→A	F82F (TTC→TTT)	xylE ←	D‑xylose:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,242,009	0	G	A	100.0%	206.3 / NA	59	F82F (TTC→TTT)	xylE	D‑xylose:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/27);  total (32/27)

TCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTG  >  NC_000913/4241872‑4242145                                                                                                                                          |                                                                                                                                         tCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCaaa                                                                                                                                        >  1:1074368/1‑140 (MQ=255)    gggACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCAAAGCg                                                                                                                                     >  2:942235/1‑140 (MQ=255)     ggACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCAAAGCgg                                                                                                                                    >  2:1162770/1‑140 (MQ=255)          tAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCAAAGCGGTTACt                                                                                                                               <  1:611564/140‑1 (MQ=255)           aaCCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCAAAGCGGTTACTg                                                                                                                              >  2:233543/1‑140 (MQ=255)           aaCCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCAAAGCGGTTACTg                                                                                                                              >  1:684948/1‑140 (MQ=255)              cTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCAAAGCGGTTACTGCaa                                                                                                                           <  1:1162770/140‑1 (MQ=255)                  cAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCAAAGCGGTTACTGCAATAac                                                                                                                       >  2:1073600/1‑140 (MQ=255)                     aTAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCAAAGCGGt                                                                                                                                   >  2:824782/1‑125 (MQ=255)                     aTAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCAAAGCGGt                                                                                                                                   >  2:699358/1‑125 (MQ=255)                     aTAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCAAAGCGGt                                                                                                                                   <  1:699358/125‑1 (MQ=255)                     aTAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCAAAGCGGt                                                                                                                                   <  1:824782/125‑1 (MQ=255)                      tAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCAAAGCGGTTACTGCAATAaccacc                                                                                                                   <  1:233543/140‑1 (MQ=255)                       aaaCAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCAAAGCGGTTACTGCAATAACCACCg                                                                                                                  >  1:349355/1‑140 (MQ=255)                         aCAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCAAAGCGGTTACTGCAATAACCACCGAg                                                                                                                <  2:490661/140‑1 (MQ=255)                           aGGCACAGTGTTGTCGGGTTTTATAGAGGAAAAACCAAGTTCTGGCCAGGCAGACCCTACACCAAAAAAAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACAAAAGCGGTTACTGCAATAACCACCGAggg                                                                                                              <  2:1130172/140‑1 (MQ=255)                              cacaGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCAAAGCGGTTACTGCAATAACCACCGAGGGCAc                                                                                                           >  2:760604/1‑140 (MQ=255)                               acaGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCAAAGCGGTTACTGCAATAACCACCGAGGGCAcc                                                                                                          >  2:856653/1‑140 (MQ=255)                                  gtgtTGTCCGGG‑TTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCAAAGCGGTTACTGCAATAACCACCGAGGGCAccgccg                                                                                                      >  2:596497/1‑140 (MQ=255)                                   tgttgtCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCAAAGCGGTTACTGCAATAACCACCGa                                                                                                                 <  2:999670/129‑1 (MQ=255)                                   tgttgtCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCAAAGCGGTTACTGCAATAACCACCGa                                                                                                                 >  1:999670/1‑129 (MQ=255)                                       gtCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCAAAGCGGTTACTGCAATACCCACCGAGGGCACCGCCgatga                                                                                                  <  2:420066/140‑1 (MQ=255)                                       gtCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCAAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCgatga                                                                                                  <  1:942235/140‑1 (MQ=255)                                       gtCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCAAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCgatga                                                                                                  >  2:678750/1‑140 (MQ=255)                                       gtCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCAAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCgatga                                                                                                  <  1:273115/140‑1 (MQ=255)                                       gtCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCAAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCgatga                                                                                                  >  1:332985/1‑140 (MQ=255)                                       gtCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCAAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCgatga                                                                                                  >  1:215059/1‑140 (MQ=255)                                       gtCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCAAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCgatga                                                                                                  <  1:688795/140‑1 (MQ=255)                                         ccGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCAAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGatgatg                                                                                                <  2:950422/140‑1 (MQ=255)                                           gggTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCAAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCa                                                                                              <  1:856653/140‑1 (MQ=255)                                                   agagGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCAAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCa                                                                                      <  2:104620/140‑1 (MQ=255)                                                       gTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCAAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAgc                                                                                  >  1:948098/1‑140 (MQ=255)                                                       gTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCAAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAgc                                                                                  >  1:951276/1‑140 (MQ=255)                                                             ccAAGTTCTGGCCAGGCAGAACCTACACCAGAAAAAAAAAACAGGACAGCAGAAATCTTAAGTGAATCACGACGACCAAAGCGGTTCCTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGc                                                                            <  2:291118/140‑1 (MQ=255)                                                                 gTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCAAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACg                                                                        >  1:305529/1‑140 (MQ=255)                                                                        ccgggCAGAACCTACCCCAAAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAACCACGACGACCAAAGCGGTTACTGCAATAACCCCCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAAcc                                                                 <  1:278598/137‑1 (MQ=255)                                                                            gCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCAAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAAcc                                                                 <  2:948483/136‑1 (MQ=255)                                                                            gCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCAAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAAcc                                                                 >  1:948483/1‑136 (MQ=255)                                                                             cAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCAAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAAc                                                            <  1:760604/140‑1 (MQ=255)                                                                             cAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCAAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAAc                                                            <  2:1074368/140‑1 (MQ=255)                                                                             cAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCAAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAAc                                                            <  1:596497/140‑1 (MQ=255)                                                                             cAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCAAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAAc                                                            <  1:1073600/140‑1 (MQ=255)                                                                                 aCCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCAAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAggg                                                        >  2:13739/1‑140 (MQ=255)                                                                                         cAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCAAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCa                                                <  2:332985/140‑1 (MQ=255)                                                                                          aGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCAAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAg                                               <  2:349355/140‑1 (MQ=255)                                                                                          aGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCAAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAg                                               >  1:311775/1‑140 (MQ=255)                                                                                            aaaTAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCAAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCg                                             >  1:386586/1‑140 (MQ=255)                                                                                                     aCAGGACAGCAGCAATCTTAAGTAAACCACGCCGACCAAAGCGGTTACTGCAATACCCCCCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACtt                                    <  2:227048/140‑1 (MQ=255)                                                                                                               agcaATCTTAAGTGAATCACGACGACCAAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTtgtg                          <  2:311775/140‑1 (MQ=255)                                                                                                                      ttAAGTGAATCACGACGACCAAAGCGGTTACTGCAATAACCCCCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGcaac                   <  2:1035030/140‑1 (MQ=255)                                                                                                                        aaGTGAATCACGACGACCAAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGcaacaa                 >  2:756093/1‑140 (MQ=255)                                                                                                                        aaGTGAATCACGACGACCAAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGcaacaa                 >  1:933085/1‑140 (MQ=255)                                                                                                                           tGAATCACGACGACCAAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGa              <  2:684948/140‑1 (MQ=255)                                                                                                                           tGAATCACGACGACCAAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGa              >  1:804939/1‑140 (MQ=255)                                                                                                                           ggAATCACGACGACCAAAGCGGTTACTGCAATAACCCCCGAGTGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACTGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGa              <  2:804939/139‑1 (MQ=255)                                                                                                                              aTCACGACGACCAAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACgg           >  1:207227/1‑140 (MQ=255)                                                                                                                              aTCACGACGACCAAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACgg           >  1:995785/1‑140 (MQ=255)                                                                                                                              aTCACGACGACCAAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACgg           >  2:958494/1‑140 (MQ=255)                                                                                                                               tCACGACGACCAAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACgtt          >  2:1083810/1‑138 (MQ=255)                                                                                                                               tCACGACGACCAAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGt          >  2:706169/1‑140 (MQ=255)                                                                                                                                   gacgacCAAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTg      >  2:1097147/1‑140 (MQ=255)                                                                                                                                      gacCAAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGt   <  2:305529/140‑1 (MQ=255)                                                                                                                                       acCAAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTg  >  2:59583/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                         TCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTG  >  NC_000913/4241872‑4242145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑