Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,375,356	A→G	V101A (GTG→GCG)	epmB ←	lysine 2,3‑aminomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,375,356	0	A	G	100.0%	212.1 / NA	59	V101A (GTG→GCG)	epmB	lysine 2,3‑aminomutase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/32);  total (27/32)

CAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTTCTCCATGCGATCGATAAATGAGCGGGG  >  NC_000913/4375219‑4375492                                                                                                                                          |                                                                                                                                         cAGTTTGCCAGTTACGCTTGTTGCCGTGATCCTCGGCATAGGGGAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCGCt                                                                                                                                        <  2:816405/140‑1 (MQ=255)      tGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCGCTACgct                                                                                                                                   <  2:669675/140‑1 (MQ=255)          aGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCGCTACgctgtgc                                                                                                                               <  1:9079/140‑1 (MQ=255)            ttACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCGCTACgctgtgctg                                                                                                                             <  2:105325/140‑1 (MQ=255)                 cTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCGCTACgctgtgctg                                                                                                                             <  1:627469/135‑1 (MQ=255)                 cTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCGCTACgctgtgctg                                                                                                                             >  2:627469/1‑135 (MQ=255)                      tgCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAg                                                                                                                    >  2:309047/1‑139 (MQ=255)                      tgCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAg                                                                                                                    <  1:309047/139‑1 (MQ=255)                      tgCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAGt                                                                                                                   >  2:616853/1‑140 (MQ=255)                      tgCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAGt                                                                                                                   >  2:15403/1‑140 (MQ=255)                           tGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGTc                                                                                                              <  2:714047/140‑1 (MQ=255)                           tGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAGAGGGTc                                                                                                              <  1:525236/140‑1 (MQ=255)                             aTTTTCGGCATAGGGGAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGTCgg                                                                                                            >  1:758/1‑140 (MQ=255)                                  cGGCATAGGGGAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGCGGTATTTATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGgag                                                                                                       >  2:310760/1‑140 (MQ=255)                                       tAGGGGAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATcc                                                                                                  >  2:67356/1‑140 (MQ=255)                                          gggAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGt                                                                                                               >  1:604254/1‑124 (MQ=255)                                          gggAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGt                                                                                                               <  2:604254/124‑1 (MQ=255)                                                    gacgGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACa                                                                                     >  1:139676/1‑140 (MQ=255)                                                     acgGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACaa                                                                                    >  1:202880/1‑140 (MQ=255)                                                         aaGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTc                                                                                >  2:404780/1‑140 (MQ=255)                                                              aTAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGTGATGACAAACTCATCtt                                                                           <  2:1124042/140‑1 (MQ=255)                                                               tAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTg                                                                          <  2:936823/140‑1 (MQ=255)                                                               tAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGCGGTATTTATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTg                                                                          <  1:310760/140‑1 (MQ=255)                                                                aGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGc                                                                         <  2:190079/140‑1 (MQ=255)                                                                  cGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTg                                                                          >  1:631093/1‑137 (MQ=255)                                                                  cGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTg                                                                          >  1:914210/1‑137 (MQ=255)                                                                  cGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTg                                                                          <  2:631093/137‑1 (MQ=255)                                                                  cGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTg                                                                          <  2:914210/137‑1 (MQ=255)                                                                       aTTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTaa                                                                  <  2:758/140‑1 (MQ=255)                                                                            ccGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTAcc                                                             <  1:671817/140‑1 (MQ=255)                                                                              gcgcAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGt                                                                    <  2:329812/131‑1 (MQ=255)                                                                              gcgcAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGt                                                                    >  1:329812/1‑131 (MQ=255)                                                                                 cAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACg                                                        <  2:1027635/140‑1 (MQ=255)                                                                                    ccgccTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCaa                                                     >  1:1123117/1‑140 (MQ=255)                                                                                              ccAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCg                                           >  2:515455/1‑140 (MQ=255)                                                                                                  cAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCg                                       >  1:405012/1‑140 (MQ=255)                                                                                                    aaaGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGa                                     <  2:405012/140‑1 (MQ=255)                                                                                                    aaaGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGa                                     <  1:1006279/140‑1 (MQ=255)                                                                                                     aaGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGAt                                    <  2:126697/140‑1 (MQ=255)                                                                                                      aGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGAtt                                   >  2:295208/1‑140 (MQ=255)                                                                                                      aGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGAtt                                   <  1:295208/140‑1 (MQ=255)                                                                                                        cgcCCGGTTGTGGTATTTATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGc                                 <  2:956589/140‑1 (MQ=255)                                                                                                            cGGTTGTGGTATTTATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCttt                             <  1:67356/140‑1 (MQ=255)                                                                                                                tgtgGTATTTATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACACAAAAGAGGATCGTCCGGATTGCCTTTCTcc                         >  1:622347/1‑140 (MQ=255)                                                                                                                     tATTTATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTTCTCCATGcg                    <  1:404780/140‑1 (MQ=255)                                                                                                                     tATTTATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTTCTCCATGcg                    <  1:465978/140‑1 (MQ=255)                                                                                                                       tttATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTTCTCCATGcgat                  >  1:493365/1‑140 (MQ=255)                                                                                                                        ttATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTTCTCCATGcgatc                 >  2:1111132/1‑140 (MQ=255)                                                                                                                         tATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTTCTCCATGcgatcg                >  2:644571/1‑140 (MQ=255)                                                                                                                         tATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTTCTCCATGcgatc                 <  1:170390/139‑1 (MQ=255)                                                                                                                         tATGCAACAAACCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTTCTCCATGcgatc                 >  2:170390/1‑139 (MQ=255)                                                                                                                            gCAACAAACCAGGCGCTACGCTGTGCTG‑‑‑TTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTTCTCCATg                      <  2:716641/128‑1 (MQ=255)                                                                                                                            gCAACAAACCAGGCGCTACGCTGTGCTG‑‑‑TTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTTCTCCATg                      >  1:716641/1‑128 (MQ=255)                                                                                                                              aacaaACCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTTCTCCATGCGATCGATaaa           >  1:613119/1‑140 (MQ=255)                                                                                                                                 aaaCCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTTCTCCATGCGATCGATAAATGa        >  2:148672/1‑140 (MQ=255)                                                                                                                                  aaCCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTTCTCCATGCGATCGATAAATGAg       <  1:948730/140‑1 (MQ=255)                                                                                                                                   aCCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTTCTCCATGCGATCGATAAATGAGc      <  1:644571/140‑1 (MQ=255)                                                                                                                                   aCCAGGCGCTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTTCTCCATGCGATCGATAAATGAGc      >  2:911337/1‑140 (MQ=255)                                                                                                                                       ggCGCTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTTCTCCATGCGATCGATAAATGAGCgggg  <  1:148672/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         CAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTTCTCCATGCGATCGATAAATGAGCGGGG  >  NC_000913/4375219‑4375492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑