Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	445,912	A→G	V252A (GTC→GCC)	yajR ←	putative major facilitator superfamily transport protein YajR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	445,912	0	A	G	100.0%	194.7 / NA	55	V252A (GTC→GCC)	yajR	putative major facilitator superfamily transport protein YajR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/21);  total (34/21)

TTCCACAACACAATTTCCGCAACCACGATCAACCCGACGCAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGCCAGCAC  >  NC_000913/445777‑446045                                                                                                                                        |                                                                                                                                      ttCCACAACACAATTTCCGCAACCACGATCAACCCGACGCAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGGCCtt                                                                                                                                   >  1:1003440/1‑140 (MQ=255)     acaacacaaTTTCCGCAACCACGATCAACCCGACGCAGAAGACAAAGACTCGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGGCCTTCCAg                                                                                                                               <  1:172211/140‑1 (MQ=255)            aaTTTCCGCAACCACGATCAACCCGACGCAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGGCCTTCCAGTGTTCAg                                                                                                                        <  2:760202/140‑1 (MQ=255)            aaTTTCCGCAACCACGATCAACCCGACGCAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGGCCTTCCAGTGTTCAg                                                                                                                        >  1:760202/1‑140 (MQ=255)                 ccGCAACCACGATCAACCCGACGCAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGGCCTTCCAGTGTTCAgccgcc                                                                                                                   >  1:809060/1‑140 (MQ=255)                   gCAACCACGATCAACCCGACGCAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGGCCTTCCAGTGTTCAGTCGCCgg                                                                                                                 >  2:218951/1‑140 (MQ=255)                      aCCACGATCAACCCGACGCAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGaa                                                                                                              >  2:809953/1‑140 (MQ=255)                        cACGATCAACCCGACGCAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGAAcc                                                                                                            <  1:394521/140‑1 (MQ=255)                           gATCAACCCGACGCAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGAACCCTg                                                                                                         <  2:511358/140‑1 (MQ=255)                                 cccGACGCAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAg                                                                                                   >  2:722313/1‑140 (MQ=255)                                  ccGACGCAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGc                                                                                                  <  1:722313/140‑1 (MQ=255)                                    gACGCAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCa                                                                                                <  1:827807/140‑1 (MQ=255)                                     aCGCAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAg                                                                                               >  2:631844/1‑140 (MQ=255)                                         agaagaCAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGt                                                                                           >  2:282595/1‑140 (MQ=255)                                           aagaCAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTcc                                                                                         >  1:518970/1‑140 (MQ=255)                                                     cTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCgggcagggca                                                                               >  2:611328/1‑140 (MQ=255)                                                       tGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGcaac                                                                             <  1:631844/140‑1 (MQ=255)                                                        gCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGGCCTTCCAGTGTTCAGCCGCCAGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGcaaca                                                                            >  1:569961/1‑140 (MQ=255)                                                                   cgcTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACAt                                                                 >  1:799484/1‑140 (MQ=255)                                                                        aaCTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGGCCTTCCAGTGCTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCa                                                            <  2:1088260/140‑1 (MQ=255)                                                                             cAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAata                                                       <  2:518970/140‑1 (MQ=255)                                                                                  tAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCa                                                               >  1:1118513/1‑127 (MQ=255)                                                                                  tAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCa                                                               <  2:1118513/127‑1 (MQ=255)                                                                                  tAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAg                                                  <  1:809953/140‑1 (MQ=255)                                                                                   aGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGa                                                 <  1:662321/140‑1 (MQ=255)                                                                                         tGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATa                                           >  1:897950/1‑140 (MQ=255)                                                                                            aaGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATg                                        >  2:240609/1‑140 (MQ=255)                                                                                             aGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGc                                       >  2:511209/1‑140 (MQ=255)                                                                                              ggCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGcc                                      >  1:664118/1‑140 (MQ=255)                                                                                              ggCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGcc                                      <  2:664118/140‑1 (MQ=255)                                                                                               gCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGc                                       >  2:94378/1‑138 (MQ=255)                                                                                               gCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGc                                       <  1:94378/138‑1 (MQ=255)                                                                                               gCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCa                                     >  1:1033619/1‑140 (MQ=255)                                                                                               gCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCa                                     >  1:1155509/1‑140 (MQ=255)                                                                                               gCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCa                                     >  1:520610/1‑140 (MQ=255)                                                                                               gCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCa                                     >  2:1164366/1‑140 (MQ=255)                                                                                                cACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCaa                                    >  2:664994/1‑140 (MQ=255)                                                                                                  cGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAg                                  >  2:512670/1‑140 (MQ=255)                                                                                                    aCCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGtt                                >  2:247277/1‑140 (MQ=255)                                                                                                        aGCCAAAGGCGATTAGCATTGTCGCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGc                                       <  2:158533/129‑1 (MQ=255)                                                                                                        aGCCAAAGGCGATTAGCATTGTCGCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGc                                       >  1:158533/1‑129 (MQ=255)                                                                                                        aGCCAAAGGCGATTAGCATTGTCGCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGt                            >  2:121787/1‑140 (MQ=255)                                                                                                         gCCAAAGGCGATTAGCATTGTCGCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGtt                           >  1:89267/1‑140 (MQ=255)                                                                                                                   aTTAGCATTGTCGCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCcg                 >  1:1077459/1‑140 (MQ=255)                                                                                                                   aTTAGCATTGTCGCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCcg                 >  2:519979/1‑140 (MQ=255)                                                                                                                   aTTAGCATTGTCGCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCcg                 >  2:617293/1‑140 (MQ=255)                                                                                                                            gTCGCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGc        <  1:240609/140‑1 (MQ=255)                                                                                                                             tCGCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGcc       <  2:89267/140‑1 (MQ=255)                                                                                                                             tCGCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGcc       >  2:1139058/1‑140 (MQ=255)                                                                                                                              cGCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGCCa      <  1:1164366/140‑1 (MQ=255)                                                                                                                               gCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGCCAg     <  1:121787/140‑1 (MQ=255)                                                                                                                               gCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGCCAg     <  1:519979/140‑1 (MQ=255)                                                                                                                               gCCAGATAGGCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGCCAg     <  2:1110103/140‑1 (MQ=255)                                                                                                                                  aGATAGGCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGCCAGCAc  >  1:473569/1‑140 (MQ=255)                                                                                                                                  aGATAGGCCTTCCAGTGTTCAACCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGCCAGCAc  >  1:766066/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                      TTCCACAACACAATTTCCGCAACCACGATCAACCCGACGCAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGCCAGCAC  >  NC_000913/445777‑446045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑