Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,547,354	T→C	Y753Y (TAT→TAC)	fimD →	type I fimbriae usher protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,547,354	0	T	C	100.0%	298.4 / NA	83	Y753Y (TAT→TAC)	fimD	type I fimbriae usher protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/45);  total (38/45)

TAACGCTGGGGCAGCCGTTAAACGATACGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATATCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGATTTAGATAACGCGGTTGCTAACGTTGTTCCCACTCGTGGGGCGATCGTGCGAGCAGAGTTTAAAGCGCGCGTTGGGATAAAACTG  >  NC_000913/4547218‑4547489                                                                                                                                         |                                                                                                                                        tAACGCTGGGGCAGCCGTTAAACGATACGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCgg                                                                                                                                      >  2:718737/1‑140 (MQ=255) tAACGCTGGGGCAGCCGTTAAACGATACGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCgg                                                                                                                                      >  2:953575/1‑140 (MQ=255)  aaCGCTGGGGCAGCCGTTAAACGATACGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCggg                                                                                                                                     >  2:176765/1‑140 (MQ=255)   aCGCTGGGGCAGCCGTTAAACGATACGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGa                                                                                                                                    <  1:270605/140‑1 (MQ=255)    cGCTGGGGCAGCCGTTAAACGATACGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGaa                                                                                                                                   >  2:104151/1‑140 (MQ=255)        ggggCAGCCGTTAAACGATACGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATa                                                                                                                               >  2:378431/1‑140 (MQ=255)         gggCAGCCGTTAAACGATACGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATag                                                                                                                              >  1:251253/1‑140 (MQ=255)           gCAGCCGTTAAACGATACGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATagag                                                                                                                            <  2:206208/140‑1 (MQ=255)           gCAGCCGTTAAACGATACGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATagag                                                                                                                            <  1:1014522/140‑1 (MQ=255)           gCAGCCGTTAAACGATACGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATagag                                                                                                                            <  1:680961/140‑1 (MQ=255)               ccGTTAAACGATACGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGgc                                                                                                                        >  1:7720/1‑140 (MQ=255)               ccGTTAAACGATACGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGgc                                                                                                                        >  2:402641/1‑140 (MQ=255)               ccGTTAAACGATACGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGgc                                                                                                                        >  2:985267/1‑140 (MQ=255)                cGTTAAACGATACGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGgcg                                                                                                                       <  1:953575/140‑1 (MQ=255)                cGTTAAACGATACGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGgcg                                                                                                                       <  1:176765/140‑1 (MQ=255)                cGTTAAACGATACGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGgcg                                                                                                                       <  1:606016/140‑1 (MQ=255)                   tAAACGATACGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGgcgc                                                                                                                      <  2:600336/138‑1 (MQ=255)                   tAAACGATACGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGgcgc                                                                                                                      >  1:600336/1‑138 (MQ=255)                   tAAACGATACGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTg                                                                                                                    >  2:286150/1‑140 (MQ=255)                   tAAACGATACGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTg                                                                                                                    <  2:859717/140‑1 (MQ=255)                     aaCGATACGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGa                                                                                                                  <  1:776217/140‑1 (MQ=255)                     aaCGATACGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGa                                                                                                                  <  1:985267/140‑1 (MQ=255)                     aaCGATACGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGa                                                                                                                  <  1:402641/140‑1 (MQ=255)                         aTACGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATAcc                                                                                                              >  1:147172/1‑140 (MQ=255)                         aTACGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATAcc                                                                                                              >  1:106560/1‑140 (MQ=255)                          tACGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCa                                                                                                             <  2:660992/140‑1 (MQ=255)                          tACGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCa                                                                                                             <  1:286150/140‑1 (MQ=255)                           aCGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCaa                                                                                                            <  1:701049/140‑1 (MQ=255)                           aCGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCaa                                                                                                            <  2:251253/140‑1 (MQ=255)                               tggtgCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATAcc                                                                                                        <  2:55833/140‑1 (MQ=255)                               tggtgCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTACTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATAcc                                                                                                        <  1:931510/140‑1 (MQ=255)                                ggtgCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATAccc                                                                                                       <  1:121301/140‑1 (MQ=255)                                  tgCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTg                                                                                                     <  2:318252/140‑1 (MQ=255)                                    cTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGa                                                                                                                  <  1:565254/125‑1 (MQ=255)                                    cTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGa                                                                                                                  >  2:565254/1‑125 (MQ=255)                                    cTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATAcc                                                                                                        >  2:1103231/1‑135 (MQ=255)                                    cTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATAcc                                                                                                        <  1:1103231/135‑1 (MQ=255)                                    cTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGc                                                                                                   >  1:962107/1‑140 (MQ=255)                                       gTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGa                                                                                                >  2:128577/1‑140 (MQ=255)                                        ttAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGAt                                                                                               <  1:378431/140‑1 (MQ=255)                                        ttAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGAt                                                                                               <  2:156978/140‑1 (MQ=255)                                            aGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACg                                                                                           >  1:647905/1‑140 (MQ=255)                                            aGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACg                                                                                           >  1:977790/1‑140 (MQ=255)                                            aGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACg                                                                                           <  1:287310/140‑1 (MQ=255)                                            aGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACg                                                                                           <  2:662325/140‑1 (MQ=255)                                            aGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACg                                                                                           <  2:147172/140‑1 (MQ=255)                                                ccTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGa                                                                                       >  2:347855/1‑140 (MQ=255)                                                ccTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGa                                                                                       >  1:946424/1‑140 (MQ=255)                                                ccTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGa                                                                                       >  1:131318/1‑140 (MQ=255)                                                ccTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGa                                                                                       >  1:53015/1‑140 (MQ=255)                                                 cTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTTGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGAt                                                                                      <  2:985760/140‑1 (MQ=255)                                                  tGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGAtt                                                                                     >  1:329563/1‑140 (MQ=255)                                                            gATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGATTTAGATAAcgc                                                                           <  2:7720/140‑1 (MQ=255)                                                             aTGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGATTTAGATAAcgcg                                                                          <  1:128577/140‑1 (MQ=255)                                                                       cGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGATTTAGATAACGCGGTTGCTAACg                                                                <  2:835266/140‑1 (MQ=255)                                                                              cAGACGGGGGTGCGTACCGACTGGCGTGGTTATTCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGATTTAGATAACGCGGTTGCTAACGTTGTTcc                                                         <  1:5887/140‑1 (MQ=255)                                                                              cAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGATTTAGATAACGCGGTTGCTAACGTTGTTcc                                                         <  2:131318/140‑1 (MQ=255)                                                                              cAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGATTTAGATAACGCGGTTGCTAACGTTGTTcc                                                         <  2:962107/140‑1 (MQ=255)                                                                              cAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGATTTAGATAACGCGGTTGCTAACGTTGTTcc                                                         <  1:654592/140‑1 (MQ=255)                                                                              cAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGATTTAGATAACGCGGTTGCTAACGTTGTTcc                                                         <  2:946424/140‑1 (MQ=255)                                                                                     gggTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGATTTAGATAACGCGGTTGCTAACGTTGTTCCCa                                                       >  1:439201/1‑135 (MQ=255)                                                                                     gggTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGATTTAGATAACGCGGTTGCTAACGTTGTTCCCa                                                       <  2:439201/135‑1 (MQ=255)                                                                                      ggTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGATTTAGATAACGCGGTTGCTAACGTTGTTCCCACTCGTg                                                 >  2:574714/1‑140 (MQ=255)                                                                                        tGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGATTTAGATAACGCGGTTGCTAACGTTGTTCCCACt                                                     <  2:509479/134‑1 (MQ=255)                                                                                        tGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGATTTAGATAACGCGGTTGCTAACGTTGTTCCCACt                                                     >  1:509479/1‑134 (MQ=255)                                                                                                 aCTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGATTTAGATAACGCGGTTGCTAACGTTGTTCCCACTCGTGGGGCGATCggg                                      >  2:240260/1‑138 (MQ=255)                                                                                                      cGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGATTTAGATAACGCGGTTGCTAACGTTGTTCCCACTCGTGGGGCGATCGTGCGAGc                                 >  2:877356/1‑140 (MQ=255)                                                                                                      cGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGATTTAGATAACGCGGTTGCTAACGTTGTTCCCACTCGTGGGGCGATCGTGCGAGc                                 <  2:977790/140‑1 (MQ=255)                                                                                                      cGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGATTTAGATAACGCGGTTGCTAACGTTGTTCCCACTCGTGGGGCGATCGTGCGAGc                                 <  2:106560/140‑1 (MQ=255)                                                                                                      cGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGATTTAGATAACGCGGTTGCTAACGTTGTTCCCACTCGTGGGGCGATCGTGCGAGc                                 >  2:936944/1‑140 (MQ=255)                                                                                                      cGTGGTTATGCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGATTTAGATAACGCGGTTGCTAACGTTGTTCCCACTCGTGGGGCGATCGTGCGAGc                                 <  2:53015/140‑1 (MQ=255)                                                                                                               gCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGATTTAGATAACGCGGTTGCTAACGTTGTTCCCACTCGTGGGGCGATCGTGCGAGc                                 <  2:689546/131‑1 (MQ=255)                                                                                                               gCCGTGCTGCCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGATTTAGATAACGCGGTTGCTAACGTTGTTCCCACTCGTGGGGCGATCGTGCGAGc                                 >  1:689546/1‑131 (MQ=255)                                                                                                                   tgctgcCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGATTTAGATAACGCGGTTGCTAACGTTGTTCCCACTCGTGGGGCGATCGTGCGAGCAGAGTTTAAAgcg                    >  2:735047/1‑140 (MQ=255)                                                                                                                    gctgcCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGATTTAGATAACGCGGTTGCTAACGTTGTTCCCACTCGTGGGGCGATCGTGCGAGCAGAGTTTAAAgcgc                   >  2:51520/1‑140 (MQ=255)                                                                                                                     ctgcCTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGATTTAGATAACGCGGTTGCTAACGTTGTTCCCACTCGTGGGGCGATCGTGCGAGCAGAGTTTAAAgcgcg                  >  1:395632/1‑140 (MQ=255)                                                                                                                        ccTTATGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGATTTAGATAACGCGGTTGCTAACGTTGTTCCCACTCGTGGGGCGATCGTGCGAGCAGAGTTTAAAGCGCGCGt               >  1:798876/1‑140 (MQ=255)                                                                                                                             tGCCACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGATTTAGATAACGCGGTTGCTAACGTTGTTCCCACTCGTGGGGCGATCGTGCGAGCAGAGTTTAAAGCGCGCGTTGGGa          >  1:184822/1‑140 (MQ=255)                                                                                                                                cACTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGATTTAGATAACGCGGTTGCTAACGTTGTTCCCACTCGTGGGGCGATCGTGCGAGCAGAGTTTAAAGCGCGCGTTGGGATaa       <  1:260878/140‑1 (MQ=255)                                                                                                                                 aCTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGATTTAGATAACGCGGTTGCTAACGTTGTTCCCACTCGTGGGGCGATCGTGCGAGCAGAGTTTAAAGCGCGCGTTGGGATaaa      <  2:395632/140‑1 (MQ=255)                                                                                                                                  cTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGATTTAGATAACGCGGTTGCTAACGTTGTTCCCACTCGTGGGGCGATCGTGCGAGCAGAGTTTAAAGCGCGCGTTGGGATaaaa     >  2:275734/1‑140 (MQ=255)                                                                                                                                  cTGAATACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGATTTAGATAACGCGGTTGCTAACGTTGTTCCCACTCGTGGGGCGATCGTGCGAGCAGAGTTTAAAGCGCGCGTTGGGATaaaa     <  2:184822/140‑1 (MQ=255)                                                                                                                                     aaTACCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGATTTAGATAACGCGGTTGCTAACGTTGTTCCCACTCGTGGGGCGATCGTGCGAGCAGAGTTTAAAGCGCGCGTTGGGATAAAACTg  >  1:1040032/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        TAACGCTGGGGCAGCCGTTAAACGATACGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATATCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGATTTAGATAACGCGGTTGCTAACGTTGTTCCCACTCGTGGGGCGATCGTGCGAGCAGAGTTTAAAGCGCGCGTTGGGATAAAACTG  >  NC_000913/4547218‑4547489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑