Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,640,270 A→G L13L (TTG→CTG)  arcA ← DNA‑binding transcriptional dual regulator ArcA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,640,2700AG98.6% 246.6 / ‑4.5 72L13L (TTG→CTG) arcADNA‑binding transcriptional dual regulator ArcA
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (41/30);  total (42/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01

CAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGATGCATGCTGCAAATTAACATGATCGGCGTAAC  >  NC_000913/4640136‑4640401
                                                                                                                                      |                                                                                                                                   
cAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGt                                                                                                                                >  1:1007940/1‑140 (MQ=255)
cAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGt                                                                                                                                >  2:524494/1‑140 (MQ=255)
          tCCATGATCACCAGGTTGATGTCACATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGAt                                                                                                                      >  2:994071/1‑140 (MQ=255)
             aTGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCt                                                                                                                              >  2:579042/1‑129 (MQ=255)
             aTGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCt                                                                                                                              <  1:579042/129‑1 (MQ=255)
             aTGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTc                                                                                                                            >  2:526588/1‑131 (MQ=255)
             aTGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTc                                                                                                                            <  1:526588/131‑1 (MQ=255)
             aTGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAg                                                                                                                   >  1:130318/1‑140 (MQ=255)
             aTGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAg                                                                                                                   >  2:823225/1‑140 (MQ=255)
               gATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGaa                                                                                                                 >  1:461698/1‑140 (MQ=255)
                 tCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAAtg                                                                                                               >  1:889617/1‑140 (MQ=255)
                    ccAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGc                                                                                                            >  1:974767/1‑140 (MQ=255)
                    ccAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGc                                                                                                            >  2:515073/1‑140 (MQ=255)
                     cAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCg                                                                                                           <  2:131654/140‑1 (MQ=255)
                     cAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCg                                                                                                           <  2:461698/140‑1 (MQ=255)
                       ggTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCggg                                                                                                         <  1:823225/140‑1 (MQ=255)
                       ggTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCggg                                                                                                         <  2:1007940/140‑1 (MQ=255)
                       ggTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCggg                                                                                                         >  1:885324/1‑140 (MQ=255)
                        gTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCgggg                                                                                                        >  2:275059/1‑140 (MQ=255)
                         ttGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGt                                                                                                       <  1:865320/140‑1 (MQ=255)
                          tGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTc                                                                                                      >  2:1033141/1‑140 (MQ=255)
                            aTGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTg                                                                                                    >  2:813824/1‑140 (MQ=255)
                            aTGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTg                                                                                                    >  2:508972/1‑140 (MQ=255)
                                    ttCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTg                                                                                            <  1:524494/140‑1 (MQ=255)
                                       agagagGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTa                                                                                         <  1:324220/140‑1 (MQ=255)
                                        gagagGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTAc                                                                                        <  2:437122/140‑1 (MQ=255)
                                         agagGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTAcc                                                                                       >  1:148534/1‑140 (MQ=255)
                                           agGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTa                                                                                     >  2:1006714/1‑140 (MQ=255)
                                             gATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTaaa                                                                                   >  1:188044/1‑140 (MQ=255)
                                              aTCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAAt                                                                                  >  1:951864/1‑140 (MQ=255)
                                              aTCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAAt                                                                                  >  2:955458/1‑140 (MQ=255)
                                              aTCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAAt                                                                                  >  2:251215/1‑140 (MQ=255)
                                               tCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAAtt                                                                                 >  2:440359/1‑140 (MQ=255)
                                                     gCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAAc                                                                           >  2:958670/1‑140 (MQ=255)
                                                        tttCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTaa                                                                        >  2:467451/1‑140 (MQ=255)
                                                        tttCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTaa                                                                        >  2:836235/1‑140 (MQ=255)
                                                                ccATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAAc                                                                >  1:288317/1‑140 (MQ=255)
                                                                 cATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACa                                                               <  2:951864/140‑1 (MQ=255)
                                                                           ttCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACaa                                                     <  1:994071/140‑1 (MQ=255)
                                                                            tCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACaaa                                                    <  2:288317/140‑1 (MQ=255)
                                                                            tCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACaaa                                                    <  1:1033141/140‑1 (MQ=255)
                                                                               aaaaCATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGt                                                 >  2:1137181/1‑140 (MQ=255)
                                                                                     tcatAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGa                                           >  2:160887/1‑140 (MQ=255)
                                                                                     tcatAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGa                                           >  1:1116749/1‑140 (MQ=255)
                                                                                     tcatAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGa                                           <  1:958670/140‑1 (MQ=255)
                                                                                      catAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGAc                                          <  2:130318/140‑1 (MQ=255)
                                                                                        tAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGCGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGAcct                                        <  2:981480/140‑1 (MQ=255)
                                                                                          gCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGAcctgc                                      <  1:440359/140‑1 (MQ=255)
                                                                                          gCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGAcctgc                                      <  2:1116749/140‑1 (MQ=255)
                                                                                          gCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGAc                                          <  1:938625/136‑1 (MQ=255)
                                                                                          gCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGAc                                          >  2:938625/1‑136 (MQ=255)
                                                                                              tCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGa                                  >  1:81117/1‑140 (MQ=255)
                                                                                              tCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGa                                  >  1:444799/1‑140 (MQ=255)
                                                                                               ccGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGat                                 >  2:911821/1‑140 (MQ=255)
                                                                                                cGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGatg                                >  2:878755/1‑140 (MQ=255)
                                                                                                  cTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGatgca                              <  2:900279/140‑1 (MQ=255)
                                                                                                  cTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGatgca                              <  1:251215/140‑1 (MQ=255)
                                                                                                                ttCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGATGCATGCTGCAAATTAAc                <  1:795114/140‑1 (MQ=255)
                                                                                                                ttCAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGATGCATGCTGCAAATTAAc                >  1:712952/1‑140 (MQ=255)
                                                                                                                  cAACGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGATGCATGCTGCAAATTAACAt              >  2:660318/1‑140 (MQ=255)
                                                                                                                    aCGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGATGCATGCTGCAAATTAACATGa            <  1:160887/140‑1 (MQ=255)
                                                                                                                    aCGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGATGCATGCTGCAAATTAACATGa            <  2:148534/140‑1 (MQ=255)
                                                                                                                     cGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGATGCATGCTGCAAATTAACATGAt           <  1:275059/140‑1 (MQ=255)
                                                                                                                     cGTGTTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGATGCATGCTGCAAATTAACATGAt           <  1:911821/140‑1 (MQ=255)
                                                                                                                       tgtTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGATGCATGCTGCAAATTAACATGATCg         >  2:872826/1‑140 (MQ=255)
                                                                                                                        gtTGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGATGCATGCTGCAAATTAACATGATCgg        >  2:486007/1‑140 (MQ=255)
                                                                                                                         ttGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGATGCATGCTGCAAATTAACATGATCGGc       >  1:142615/1‑140 (MQ=255)
                                                                                                                          tGCGTGTTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGATGCATGCTGCAAATTAACATGATCGGCg      >  2:289242/1‑140 (MQ=255)
                                                                                                                             gtgtTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGATGCATGCTGCAAATTAACATGATCGGCGTaa   <  2:889617/140‑1 (MQ=255)
                                                                                                                             gtgtTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGATGCATGCTGCAAATTAACATGATCGGCGTaa   <  2:885324/140‑1 (MQ=255)
                                                                                                                              tgtTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGATGCATGCTGCATATTAACATGATCGGCGTAAc  <  2:188044/140‑1 (MQ=255)
                                                                                                                              tgtTACCAGCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGATGCATGCTGCAAATTAACATGATCGGCGTAAc  >  2:1105591/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                   
CAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGATGCATGCTGCAAATTAACATGATCGGCGTAAC  >  NC_000913/4640136‑4640401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑