Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	450,380	C→T	A88T (GCG→ACG)	cyoB ←	cytochrome bo3 ubiquinol oxidase subunit 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	450,380	0	C	T	100.0%	287.8 / NA	81	A88T (GCG→ACG)	cyoB	cytochrome bo3 ubiquinol oxidase subunit 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/45);  total (36/45)

CCGATCTGCAGCGGAACCACCAGGTTCATCAGACCGATAACGAAAGGCATCGCCACGAAGAAGATCATAATCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGCCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATACATGATACCGAGGCGTTTATGGTCGACGGAGGTCAGCCACTCTTTCCA  >  NC_000913/450247‑450509                                                                                                                                      |                                                                                                                                  ccGATCTGCAGCGGAACCACCAGGTTCATCAGACCGATAACGAAAGGCATCGCCACGAAGAAGATCATAATCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGc                                                                                                                             <  2:815288/140‑1 (MQ=255)  cGATCTGCAGCGGAACCACCAGGTTCATCAGACCGATAACGAAAGGCATCGCCACGAAGAAGATCATAATCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCa                                                                                                                            <  1:268796/140‑1 (MQ=255)  cGATCTGCAGCGGAACCACCAGGTTCATCAGACCGATAACGAAAGGCATCGCCACGAAGAAGATCATAATCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCa                                                                                                                            >  2:379605/1‑140 (MQ=255)    aTCTGCAGCGGAACCACCAGGTTCATCAGACCGATAACGAAAGGCATCGCCACGAAGAAGATCATAATCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAag                                                                                                                          <  1:284343/140‑1 (MQ=255)      cTGCAGCGGAACCACCAGGTTCATCAGACCGATAACGAAAGGCATCGCCACGAAGAAGATCATAATCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAagag                                                                                                                        <  1:551019/140‑1 (MQ=255)              gAACCACCAGGTTCATCAGACCGATAACGAAAGGCATCGCCACGAAGAAGATCATAATCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCctgctag                                                                                                                <  2:1105612/140‑3 (MQ=255)              gAACCACCAGGTTCATCAGACCGATAACGAAAGGCATCGCCACGAAGAAGATCATAATCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTgg                                                                                                                >  1:686567/1‑140 (MQ=255)              gAACCACCAGGTTCATCAGACCGATAACGAAAGGCATCGCCACGAAGAAGATCATAATCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTgg                                                                                                                >  2:409736/1‑140 (MQ=255)                accaccAGGTTCATCAGACCGATAACGAAAGGCATCGCCACGAAGAAGATCATAATCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCt                                                                                                              >  2:498486/1‑140 (MQ=255)                 ccaccaGGTTCATCAGACCGATAACGAAAGGCATCGCCACGAAGAAGATCATAATCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTa                                                                                                             >  1:1121873/1‑140 (MQ=255)                 ccaccaGGTTCATCAGACCGATAACGAAAGGCATCGCCACGAAGAAGATCATAATCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTa                                                                                                             >  2:487729/1‑140 (MQ=255)                   accaGGTTCATCAGACCGATAACGAAAGGCATCGCCACGAAGAAGATCATAATCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACg                                                                                                           <  1:1091214/140‑1 (MQ=255)                     caGGTTCATCAGACCGATAACGAAAGGCATCGCCACGAAGAAGATCATAATCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGca                                                                                                         <  1:379605/140‑1 (MQ=255)                        gTTCATCAGACCGATAACGAAAGGCATCGCCACGAAGAAGATCATAATCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCctg                                                                                                                    <  2:1131738/126‑1 (MQ=255)                        gTTCATCAGACCGATAACGAAAGGCATCGCCACGAAGAAGATCATAATCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCctg                                                                                                                    >  1:1131738/1‑126 (MQ=255)                         ttCATCAGACCGATAACGAAAGGCATCGCCACGAAGAAGATCATAATCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGcatcat                                                                                                     >  1:170903/1‑140 (MQ=255)                          tcatcaGACCGATAACGAAAGGCATCGCCACGAAGAAGATCATAATCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATa                                                                                                    <  2:976542/140‑1 (MQ=255)                            atcaGACCGATAACGAAAGGCATCGCCACGAAGAAGATCATAATCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAAt                                                                                                  >  1:1155290/1‑140 (MQ=255)                            atcaGACCGATAACGAAAGGCATCGCCACGAAGAAGATCATAATCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAAt                                                                                                  >  1:1108313/1‑140 (MQ=255)                               aGACCGATAACGAAAGGCATCGCCACGAAGAAGATCATAATCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGc                                                                                               <  2:559363/140‑1 (MQ=255)                               aGACCGATAACGAAAGGCATCGCCACGAAGAAGATCATAATCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGc                                                                                               <  1:1030260/140‑1 (MQ=255)                                    gATAACGAAAGGCATCGCCACGAAGAAGATCATAATCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAg                                                                                          <  2:641531/140‑1 (MQ=255)                                         cGAAAGGCATCGCCACGAAGAAGATCATAATCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCaaaa                                                                                     <  2:75537/140‑1 (MQ=255)                                            aaGGCATCGCCACGAAGAAGATCATAATCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCa                                                                                  <  2:1156673/140‑1 (MQ=255)                                            aaGGCATCGCCACGAAGAAGATCATAATCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCa                                                                                  <  1:743148/140‑1 (MQ=255)                                             aGGCATCGCCACGAAGAAGATCATAATCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCAc                                                                                 >  2:310135/1‑140 (MQ=255)                                               gCATCGCCACGAAGAAGATCATAATCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACgc                                                                               >  1:88577/1‑140 (MQ=255)                                                 aTCGCCACGAAGAAGATCATAATCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACgcag                                                                             <  2:170903/140‑1 (MQ=255)                                                  tCGCCACGAAGAAGATCATAATCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACgcagc                                                                            <  2:961322/140‑1 (MQ=255)                                                  tCGCCACGAAGAAGATCATAATCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACgcagc                                                                            <  1:498486/140‑1 (MQ=255)                                                                  cATAATCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCa                                                            <  2:212924/140‑1 (MQ=255)                                                                  cATAATCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCa                                                            <  1:1153534/140‑1 (MQ=255)                                                                    tAATCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACg                                                          <  1:418127/140‑1 (MQ=255)                                                                    tAATCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACg                                                          >  2:288774/1‑140 (MQ=255)                                                                    tAATCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACg                                                          <  1:487729/140‑1 (MQ=255)                                                                     aaTCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACga                                                         >  2:469805/1‑140 (MQ=255)                                                                     aaTCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACga                                                         <  2:605843/140‑1 (MQ=255)                                                                     aaTCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACga                                                         >  2:793561/1‑140 (MQ=255)                                                                     aaTCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACga                                                         <  2:88577/140‑1 (MQ=255)                                                                     aaTCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACga                                                         <  1:409736/140‑1 (MQ=255)                                                                     aaTCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACga                                                         <  1:963320/140‑1 (MQ=255)                                                                       tCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACga                                                         <  2:1028812/138‑1 (MQ=255)                                                                       tCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACga                                                         >  1:1028812/1‑138 (MQ=255)                                                                         aCGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACgatgat                                                     <  1:1126533/140‑1 (MQ=255)                                                                          cGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGc                                                              <  2:591498/130‑1 (MQ=255)                                                                          cGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGc                                                              >  1:478634/1‑130 (MQ=255)                                                                          cGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGc                                                              <  2:478634/130‑1 (MQ=255)                                                                          cGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGc                                                              >  1:591498/1‑130 (MQ=255)                                                                          cGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACgatgat                                                     <  1:250397/139‑1 (MQ=255)                                                                          cGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACgatgat                                                     >  2:250397/1‑139 (MQ=255)                                                                          cGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGGGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGATCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGata                                                    >  2:833568/1‑140 (MQ=255)                                                                           gCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGatat                                                   >  1:236787/1‑140 (MQ=255)                                                                            ccGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACg                                                          >  2:212050/1‑132 (MQ=255)                                                                            ccGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACg                                                          <  1:212050/132‑1 (MQ=255)                                                                             cGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCa                                                            <  1:1108018/129‑1 (MQ=255)                                                                             cGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCa                                                            >  2:1108018/1‑129 (MQ=255)                                                                               tGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCa                                                            <  2:1040679/127‑1 (MQ=255)                                                                               tGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCa                                                            >  1:1040679/1‑127 (MQ=255)                                                                               tGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCa                                                            <  2:838577/127‑1 (MQ=255)                                                                               tGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCa                                                            >  1:838577/1‑127 (MQ=255)                                                                               tGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATACAt                                               >  1:879075/1‑140 (MQ=255)                                                                                     gTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATACATGATAcc                                         <  1:469805/140‑1 (MQ=255)                                                                                     gTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATACATGATAcc                                         <  1:793561/140‑1 (MQ=255)                                                                                        aaGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATACATGATACCGAg                                      <  2:1121873/140‑1 (MQ=255)                                                                                         aGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATACATGATACCGAgg                                     <  2:686567/140‑1 (MQ=255)                                                                                                aTCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATACATGATACCGAGGCGTTTAt                              >  1:759179/1‑140 (MQ=255)                                                                                                   gTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATACATGATACCGAGGCGTTTATGGt                           <  1:288774/140‑1 (MQ=255)                                                                                                     aGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATACATGATACCGAGGCGTTTATGGTCg                         >  2:520221/1‑140 (MQ=255)                                                                                                          tgAGGTGGCATGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATACATGATACCGAGGCGTTTATGGTCGACGGa                    >  2:174732/1‑140 (MQ=255)                                                                                                          tgAGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATACATGATACCGAGGCGTTTATGGTCGACGGa                    >  2:610138/1‑140 (MQ=255)                                                                                                            aGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATACATGATACCGAGGCGTTTATGGTCGACGGAgg                  >  1:508147/1‑140 (MQ=255)                                                                                                            aGGTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATACATGATACCGAGGCGTTTATGGTCGACGGAgg                  >  1:425719/1‑140 (MQ=255)                                                                                                              gTGGCCGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATACATGATACCGAGGCGTTTATGGTCGACGGAGGTc                >  1:645474/1‑140 (MQ=255)                                                                                                              gTGGCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATACATGATACCGAGGCGTTTATGGTCGACGGAGGTc                >  1:313056/1‑140 (MQ=255)                                                                                                                 gCAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATACATGATACCGAGGCGTTTATGGTCGACGGAGGTCAGc             >  2:460246/1‑140 (MQ=255)                                                                                                                  cAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATACATGATACCGAGGCGTTTATGGTCGACGGAGGTCAGcc            <  1:520221/140‑1 (MQ=255)                                                                                                                  cAGGAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATACATGATACCGAGGCGTTTATGGTCGACGGAGGTCAGcc            <  1:310135/140‑1 (MQ=255)                                                                                                                    ggAAGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATACATGATACCGAGGCGTTTATGGTCGACGGAGGTCAGCCAc          >  2:523665/1‑140 (MQ=255)                                                                                                                     gaggCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCAACGCATCATAATGGCGTCAGCAAAACCGCGCAGCAACATCCCAATCGCCACGATAATATACATGATACCGAGGCGTTTATGGTCGACGGAGGTCAGCCAct         <  2:614263/137‑1 (MQ=255)                                                                                                                      aaGCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATACATGATACCGAGGCGTTTATGGTCGACGGAGGTCAGCCActc        <  1:523665/140‑1 (MQ=255)                                                                                                                        gCCCGCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATACATGATACCGAGGCGTTTATGGTCGACGGAGGTCAGCCACTCtt      >  2:504233/1‑140 (MQ=255)                                                                                                                            gCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATACATGATACCGAGGCGTTTATGGTCGACGGAGGTCAGCCACTCTTTCca  <  1:460246/140‑1 (MQ=255)                                                                                                                            gCTTCGCCCGTCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATACATGATACCGAGGCGTTTATGGTCGACGGAGGTCAGCCACTCTTTCca  <  2:1155290/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                  CCGATCTGCAGCGGAACCACCAGGTTCATCAGACCGATAACGAAAGGCATCGCCACGAAGAAGATCATAATCACGCCGTGCGCGGTAAAGATCTGATCGTAGTGGTGAGGTGGCAGGAAGCCCGCTTCGCCCGCCGAGGCAAGAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATACATGATACCGAGGCGTTTATGGTCGACGGAGGTCAGCCACTCTTTCCA  >  NC_000913/450247‑450509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑