Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	479,289	A→G	intergenic (‑38/+78)	ylaC ← / ← maa	putative inner membrane protein/maltose O‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	479,289	0	A	G	100.0%	191.6 / NA	54	intergenic (‑38/+78)	ylaC/maa	putative inner membrane protein/maltose O‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/28);  total (26/28)

TACGGATAAAACTGATACTAAAGCGGGGTTTGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGGAACACCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGAAAAACCGATTACAATTTTTTAATTATTCTGGCTGGATTACCGCCCACGACAACGTTGTCC  >  NC_000913/479152‑479418                                                                                                                                          |                                                                                                                                  tACGGATAAAACTGATACTAAAGCGGGGTTTGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGGAACGcc                                                                                                                                 >  2:1132124/1‑140 (MQ=255)           aCTGATACTAAAGCGGGGTTTGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAAc                                                                                                                       <  2:949685/140‑1 (MQ=255)             tGATACTAAAGCGGGGTTTGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGt                                                                                                                     <  2:1037434/140‑1 (MQ=255)             tGATACTAAAGCGGGGTTTGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGt                                                                                                                     <  1:1014562/140‑1 (MQ=255)             tGATACTAAAGCGGGGTTTGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGt                                                                                                                     <  2:348259/140‑1 (MQ=255)                tACTAAAGCGGGGTTTGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTACCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTggg                                                                                                                  >  2:390678/1‑140 (MQ=255)                  cTAAAGCGGGGTTTGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGaa                                                                                                                <  2:623154/140‑1 (MQ=255)                   tAAAGCGGGGTTTGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGAAt                                                                                                               <  2:751174/140‑1 (MQ=255)                     aaGCGGGGTTTGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGaa                                                                                                                <  1:350425/137‑1 (MQ=255)                     aaGCGGGGTTTGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGaa                                                                                                                >  2:350425/1‑137 (MQ=255)                     aaGCGGGGTTTGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGAAtag                                                                                                             >  1:430963/1‑140 (MQ=255)                      aGCGGGGTTTGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGAAtagt                                                                                                            <  1:554170/140‑1 (MQ=255)                       gCGGGGTTTGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGAAtagta                                                                                                           >  2:794599/1‑140 (MQ=255)                       gCGGGGTTTGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGAAtagta                                                                                                           >  1:895138/1‑140 (MQ=255)                         ggggTTTGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGAATAGTaga                                                                                                         <  2:848432/140‑1 (MQ=255)                         ggggTTTGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGAATAGTaga                                                                                                         >  2:413300/1‑140 (MQ=255)                           ggTTTGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGAATAGTAGAgg                                                                                                       <  1:413300/140‑1 (MQ=255)                            gTTTGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTACCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGAATAGTAGAggg                                                                                                      <  1:390678/140‑1 (MQ=255)                             tttGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGAATAGTAGAgggg                                                                                                     >  1:428622/1‑140 (MQ=255)                             tttGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGAATAGTAGAgggg                                                                                                     <  2:69307/140‑1 (MQ=255)                             tttGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGAATAGTAGAgggg                                                                                                     <  1:303154/140‑1 (MQ=255)                             tttGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGAATAGTAGAgggg                                                                                                     >  1:1090949/1‑140 (MQ=255)                                   gtCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTaa                                                                                               <  1:1014546/140‑1 (MQ=255)                                       cgTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGAATAGGAGAGGGGAAGTAACAGa                                                                                           >  2:425929/1‑140 (MQ=255)                                                 gggTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACaa                                                                                 <  2:610896/140‑1 (MQ=255)                                                 gggTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACaa                                                                                 <  2:412397/140‑1 (MQ=255)                                                 gggTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACaa                                                                                 <  2:428622/140‑1 (MQ=255)                                                   gTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAAtt                                                                               <  2:140198/140‑1 (MQ=255)                                                    tATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAAttt                                                                              >  2:736420/1‑140 (MQ=255)                                                             aCTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACca                                                                                    <  2:575821/125‑1 (MQ=255)                                                             aCTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACca                                                                                    >  1:575821/1‑125 (MQ=255)                                                             aCTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACa                                                                     >  1:1010954/1‑140 (MQ=255)                                                                cAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAGCGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGtt                                                                  >  1:785366/1‑140 (MQ=255)                                                                 aaTCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTg                                                                 >  1:1156156/1‑140 (MQ=255)                                                                       ttCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGaaaa                                                           >  2:188374/1‑140 (MQ=255)                                                                            tCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGAAAAACCGa                                                      <  1:736420/140‑1 (MQ=255)                                                                                 agGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGAAAAACCGATTACa                                                 <  2:1156156/140‑1 (MQ=255)                                                                                      ttGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGAAAAACCGATTACAGtttt                                            >  2:1077007/1‑140 (MQ=255)                                                                                                   aTCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGAAAAACCGATTACAATTTTTTAATTATTctgg                               >  1:119169/1‑140 (MQ=255)                                                                                                     cGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGAAAAACCGATTACAATTTTTTAATTATTctggct                             >  2:494892/1‑140 (MQ=255)                                                                                                         tGCCTTCGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGAAAAACCGATTACAATTTTTTAATTATTCTGGCTGGAt                         >  2:816592/1‑140 (MQ=255)                                                                                                           ccTTCGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGAATAGGAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGAAAAACCGATTACAATTTTTTAATTATTCTGGCTGGATTa                       <  1:425929/140‑1 (MQ=255)                                                                                                             ttCGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGAAAAACCGATTACAATTTTTTAATTATTCTGGCTGGATTAcc                     >  1:1160437/1‑140 (MQ=255)                                                                                                              tCGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGAAAAACCGATTACAATTTTTTAATTATTCTGGCTGGATTACCg                    <  2:895138/140‑1 (MQ=255)                                                                                                               cGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGAAAAACCGATTACAATTTTTTAATTATTctggct                             <  2:124589/130‑1 (MQ=255)                                                                                                               cGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGAAAAACCGATTACAATTTTTTAATTATTctggct                             >  1:124589/1‑130 (MQ=255)                                                                                                               cGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGAAAAACCGATTACAATTTTTTAATTATTCTGGCTGGATTACCGc                   >  1:38569/1‑140 (MQ=255)                                                                                                               cGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGAAAAACCGATTACAATTTTTTAATTATTCTGGCTGGATTACCGc                   >  2:607219/1‑140 (MQ=255)                                                                                                               cGTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGAAAAACCGATTACAATTTTTTAATTATTCTGGCTGGATTACCGc                   >  1:664920/1‑140 (MQ=255)                                                                                                                gTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGAAAAACCGATTACAATTTTTTAATTATTCTGGCTGGATTACCGcc                  <  1:535386/140‑1 (MQ=255)                                                                                                                gTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGAAAAACCGATTACAATTTTTTAATTATTCTGGCTGGATTACCGcc                  <  2:664920/140‑1 (MQ=255)                                                                                                                gTGGTATCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGAAAAACCGATTACAATTTTTTAATTATTCTGGCTGGATTACCGcc                  <  1:816592/140‑1 (MQ=255)                                                                                                                     aTCTGCAACTTTCCAGGGAACGCCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGAAAAACCGATTACAATTTTTTAATTATTCTGGCTGGATTACCGCCCACGa             <  1:1132124/140‑1 (MQ=255)                                                                                                                                tCCAGGGAACGCCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGAAAAACCGATTACAATTTTTTAATTATTCTGGCTGGATTACCGCCCACGACAACGTTGTcc  >  2:122014/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                  TACGGATAAAACTGATACTAAAGCGGGGTTTGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGGAACACCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGAAAAACCGATTACAATTTTTTAATTATTCTGGCTGGATTACCGCCCACGACAACGTTGTCC  >  NC_000913/479152‑479418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑