| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 527,577 | T→C | intergenic (+36/‑4) | rhsD → / → ybbC | protein RhsD/PF15631 family protein YbbC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 527,577 | 0 | T | C | 98.2% | 199.7 / NA | 57 | intergenic (+36/‑4) | rhsD/ybbC | protein RhsD/PF15631 family protein YbbC |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (29/27); minor base G (0/1); total (29/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGATTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACT > NC_000913/527442‑527713 | tGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCaa < 1:132430/140‑1 (MQ=255)tGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCaa < 1:779764/140‑1 (MQ=255) ggACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGATTTTATTAGTGGGGCCTATAAGGAGACTCAATGaa > 2:806216/1‑140 (MQ=255) cGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAata < 2:630268/140‑1 (MQ=255) tttGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAg < 1:806216/140‑1 (MQ=255) ttAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATAttt > 2:91564/1‑140 (MQ=255) ttAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATAttt > 2:489335/1‑140 (MQ=255) taataaAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGa < 1:703489/140‑1 (MQ=255) aaGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCtt > 1:78284/1‑140 (MQ=255) gaATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCAtt > 2:372168/1‑140 (MQ=255) gaATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCAtt > 2:892947/1‑140 (MQ=255) aaTATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTc > 2:335819/1‑136 (MQ=255) aaTATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTc < 1:335819/136‑1 (MQ=255) aaTATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCAttt > 1:644107/1‑140 (MQ=255) aaTATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCAttt > 1:248423/1‑140 (MQ=255) tatTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTta < 2:248423/140‑1 (MQ=255) tatTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTta < 1:674747/140‑1 (MQ=255) tatTTTTATCAGAAATACTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGAGTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTta < 2:1041581/140‑1 (MQ=255) ttttATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATAc > 1:1001714/1‑140 (MQ=255) tCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTAttt > 2:531157/1‑140 (MQ=255) cAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTg < 1:91564/140‑1 (MQ=255) aGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATCCTTTCATTTTTTATACTATTTGc < 1:768885/140‑1 (MQ=255) ccGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGAc > 2:1171882/1‑140 (MQ=255) gATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCt < 2:1001714/140‑1 (MQ=255) gATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCt < 1:489335/140‑1 (MQ=255) tGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGtt < 2:933823/140‑1 (MQ=255) gAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGttt > 2:387017/1‑140 (MQ=255) aGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTa < 1:531157/140‑1 (MQ=255) gCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCtgat > 2:857207/1‑140 (MQ=255) aTACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCtgatga < 2:644107/140‑1 (MQ=255) ccGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGaaaa > 1:567677/1‑140 (MQ=255) gTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTa > 1:223582/1‑123 (MQ=255) gTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTa < 2:223582/123‑1 (MQ=255) gTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGaa > 2:605569/1‑136 (MQ=255) gTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGaa < 1:605569/136‑1 (MQ=255) gTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACa > 1:305703/1‑140 (MQ=255) aTCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACatt > 1:138033/1‑140 (MQ=255) cATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACattat < 2:305703/140‑1 (MQ=255) aTGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACattatt < 1:892947/140‑1 (MQ=255) ttAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTa > 1:94159/1‑140 (MQ=255) ttAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTa > 2:465607/1‑140 (MQ=255) aaTTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATg > 1:939406/1‑140 (MQ=255) aaTTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATg > 2:266857/1‑140 (MQ=255) aaTTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATg < 1:321833/140‑1 (MQ=255) ttttAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAg < 2:78284/140‑1 (MQ=255) tGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACa > 2:807793/1‑140 (MQ=255) gggCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACAtt < 1:807793/140‑1 (MQ=255) ggCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACAttt < 1:77008/140‑1 (MQ=255) ccTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAg > 1:263983/1‑140 (MQ=255) ccTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAg > 1:2700/1‑140 (MQ=255) cTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGa > 1:952979/1‑140 (MQ=255) taAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATaa > 1:79244/1‑140 (MQ=255) ggAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATa > 2:17926/1‑140 (MQ=255) agaCTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATAct < 1:449821/140‑1 (MQ=255) agaCTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATAct < 1:372168/140‑1 (MQ=255) agaCTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATAct < 1:387017/140‑1 (MQ=255) agaCTCAAAGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAGATAct < 1:1120718/140‑1 (MQ=255) | TGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGATTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACT > NC_000913/527442‑527713 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |