Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	622,253	G→A	intergenic (‑64/‑47)	fepD ← / → entS	ferric enterobactin ABC transporter membrane subunit FebD/enterobactin exporter EntS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	622,253	0	G	A	100.0%	119.0 / NA	34	intergenic (‑64/‑47)	fepD/entS	ferric enterobactin ABC transporter membrane subunit FebD/enterobactin exporter EntS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/14);  total (20/14)

GTCGCGATAATCAGTAATAACAGCAATCCGGGCACGGCAATGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCTAAGAATAGGTATTTAAAATATTTGATGGCAAGGCATTGTAATGAATAAACAATCCTGGCTGCTTAACCTCAGCCTGTTGAAAACGCACCCGGCGTTTCGCGCAGTATTCCTCGCTCGTTTCATCTCAAT  >  NC_000913/622119‑622388                                                                                                                                       |                                                                                                                                        gTCGCGATAATCAGTAATAACAGCAATCCGGGCACGGCAATGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTACAGcc                                                                                                                                    >  2:369875/1‑140 (MQ=255) gTCGCGATAATCAGTAATAACAGCAATCCGGGCACGGCAATGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTACAGcc                                                                                                                                    >  2:191830/1‑140 (MQ=255) gTCGCGATAATCAGTAATAACAGCAATCCGGGCACGGCAATGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTACAGcc                                                                                                                                    >  1:403984/1‑140 (MQ=255) gTCGCGATAATCAGTAATAACAGCAATCCGGGCACGGCAATGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTACAGcc                                                                                                                                    <  1:661100/140‑1 (MQ=255)  tCGCGATAATCAGTAATAACAGCAATCCGGGCACGGCAATGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTACAGCCt                                                                                                                                   >  2:773467/1‑140 (MQ=255)  tCGCGATAATCAGTAATAACAGCAATCCGGGCACGGCAATGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTACAGCCt                                                                                                                                   <  1:773467/140‑1 (MQ=255)        tAATCAGTAATAACAGCAATCCGGGCACGGCAATGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTACAGCCTAAGAAt                                                                                                                             <  2:1169125/140‑1 (MQ=255)         aaTCAGTAATAACAGCAATCCGGGCACGGCAATGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTACAGCCTAAGAATa                                                                                                                            <  2:772331/140‑1 (MQ=255)               taataaCAGCAATCCGGGCACGGCAATGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTACAGCCTAAGAATAGGTAtt                                                                                                                      <  2:100715/140‑1 (MQ=255)                   aaCAGCAATCCGGGCACGGCAATGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTACAGCCTAAGAATAGGTATTTaaa                                                                                                                  <  1:191830/140‑1 (MQ=255)                        cAATCCGGGCACGGCAATGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTACAGCCTAAGAATAGGTATTTAAAATAtt                                                                                                             >  1:956350/1‑140 (MQ=255)                         aaTCCGGGCACGGCAATGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTACAGCCTAAGAATAGGTAtt                                                                                                                      <  2:271094/130‑1 (MQ=255)                         aaTCCGGGCACGGCAATGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTACAGCCTAAGAATAGGTAtt                                                                                                                      >  1:271094/1‑130 (MQ=255)                          aTCCGGGCACGGCAATGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTACAGCCTAAGAATAGGTATTTAAAATATTTg                                                                                                           >  2:617234/1‑140 (MQ=255)                                gCACGGCAATGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTACAGCCTAAGAATAGGTATTTAAAATATTTGATGGCa                                                                                                     <  2:169524/140‑1 (MQ=255)                                     gCAATGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTACAGCCTAAGAATAGGTATTTAAAATATTTGATGGCAAGGCa                                                                                                <  2:469897/140‑1 (MQ=255)                                                tgtCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTACAGCCTAAGAATAGGTATTTAAAATATTTGATGGCAAGGCATTGTAATGAAt                                                                                     >  2:385621/1‑140 (MQ=255)                                                          aCAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTACAGCCTAAGAATAGGTATTTAAAATATTTGATGGCAAGGCATTGTAATGAATAAACAATCCt                                                                           <  1:385621/140‑1 (MQ=255)                                                           cAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTACAGCCTAAGAATAGGTATTTAAAATATTTGATGGCAAGGCATTGTAATGa                                                                                       <  1:450400/127‑1 (MQ=255)                                                           cAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTACAGCCTAAGAATAGGTATTTAAAATATTTGATGGCAAGGCATTGTAATGa                                                                                       >  2:450400/1‑127 (MQ=255)                                                                 cAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTACAGCCTAAGAATAGGTATTTAAAATATTTGATGGCAAGGCATTGTAATGAATAAACAATCCTGgctgct                                                                    <  2:79996/140‑1 (MQ=255)                                                                        aCGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTACAGCCTAAGAACAGGTATTTAAAATATTTGATGGCAAGGCATTGTAATGAATAAACAATCCTGGCTGCTTAACCTc                                                             >  1:138770/1‑140 (MQ=255)                                                                          gAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTACAGCCTAAGAATAGGTATTTAAAATATTTGATGGCAAGGCATTGTAATGAATAAACAATCCTGGCTGCTTAACCTCAg                                                           >  1:1163436/1‑140 (MQ=255)                                                                             cTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTACAGCCTAAGAATAGGTATTTAAAATATTTGATGGCAAGGCATTGTAATGAATAAACAATCCTGGCTGCTTAACCTCAGCCt                                                        >  2:728368/1‑140 (MQ=255)                                                                              ttCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTACAGCCTAAGAATAGGTATTTAAAATATTTGATGGCAAGGCATTGTAATGAATAAACAATCCTGGCTGCTTAACCTCAGCCTg                                                       >  2:147485/1‑140 (MQ=255)                                                                                                     tcgttatcgATCTTATTTGGATATGTTAGCATGTACAGCCTAAGAATAGGTATTTAAAATATTTGATGGCAAGGCATTGTAATGAATAAACAATCCTGGCTGCTTAACCTCAGCCTGTTGAAAACGCACCCGGCGTTTcg                                >  2:143630/1‑140 (MQ=255)                                                                                                        ttatcgATCTTATTTGGATATGTTAGCATGTACAGCCTAAGAATAGGTATTTAAAATATTTGATGGCAAGGCATTGTAATGAATAAACAATCCTGGCTGCTTAACCTCAGCCTGTTGAAAACGCACCCGGCGTTTCgcgc                             <  2:1163436/140‑1 (MQ=255)                                                                                                          atcgATCTTATTTGGATATGTTAGCATGTACAGCCTAAGAATAGGTATTTAAAATATTTGATGGCAAGGCATTGTAATGAATAAACAATCCTGGCTGCTTAACCTCAGCCTGTTGAAAACGCACCCGGCGTTTCGCGCAg                           >  2:94997/1‑140 (MQ=255)                                                                                                          atcgATCTTATTTGGATATGTTAGCATGTACAGCCTAAGAATAGGTATTTAAAATATTTGATGGCAAGGCATTGTAATGAATAAACAATCCTGGCTGCTTAACCTCAGCCTGTTGAAAACGCACCCGGCGTTTCGCGCAg                           >  2:965927/1‑140 (MQ=255)                                                                                                                             gTTAGCATGTACAGCCTAAGAATAGGTATTTAAAATATTTGATGGCAAGGCATTGTAATGAATAAACAATCCTGGCTGCTTAACCTCAGCCTGTTGAAAACGCACCCGGCGTTTCGCGCAGTATTCCTCGCTCGTTTCAt        >  2:554374/1‑140 (MQ=255)                                                                                                                              ttAGCATGTACAGCCTAAGAATAGGTATTTAAAATATTTGATGGCAAGGCATTGTAATGAATAAACAATCCTGGCTGCTTAACCTCAGCCTGTTGAAAACGCACCCGGCGTTTCGCGCAGTATTCCTCGCTCGTTTCAtc       <  1:49778/140‑1 (MQ=255)                                                                                                                                aGCATGTACAGCCTAAGAATAGGTATTTAAAATATTTGATGGCAAGGCATTGTAATGAATAAACAATCCTGGCTGCTTAACCTCAGCCTGTTGAAAACGCACCCGGCGTTTCGCGCAGTATTCCTCGCTCGTTTCAtctc     >  2:995267/1‑140 (MQ=255)                                                                                                                                  cATGTACAGCCTAAGAATAGGTATTTAAAATATTTGATGGCAAGGCATTGTAATGAATAAACAATCCTGGCTGCTTAACCTCAGCCTGTTGAAAACGCACCCGGCGTTTCGCGCAGTATTCCTCGCTCGTTTCATCTCaa   >  1:511382/1‑140 (MQ=255)                                                                                                                                   aTGTACAGCCTAAGAATAGGTATTTAAAATATTTGATGGCAAGGCATTGTAATGAATAAACAATCCTGGCTGCTTAACCTCAGCCTGTTGAAAACGCACCCGGCGTTTCGCGCAGTATTCCTCGCTCGTTTCATCTCAAt  >  1:144728/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        GTCGCGATAATCAGTAATAACAGCAATCCGGGCACGGCAATGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCTAAGAATAGGTATTTAAAATATTTGATGGCAAGGCATTGTAATGAATAAACAATCCTGGCTGCTTAACCTCAGCCTGTTGAAAACGCACCCGGCGTTTCGCGCAGTATTCCTCGCTCGTTTCATCTCAAT  >  NC_000913/622119‑622388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑