Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	709,153	A→G	T274A (ACC→GCC)	chiP →	chitobiose outer membrane channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	709,153	0	A	G	100.0%	200.3 / NA	57	T274A (ACC→GCC)	chiP	chitobiose outer membrane channel
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/20);  total (37/20)

CTTCAAAAATAACTTCGTACTGGAAGCGGCATTTGGTCAGGCGGAAGGGTATATCGATCAATATTTTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTACCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACG  >  NC_000913/709020‑709288                                                                                                                                      |                                                                                                                                        cTTCAAAAATAACTTCGTACTGGAAGCGGCATTTGGTCAGGCGGAAGGGTATATCGATCAATATTTTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTGcc                                                                                                                                       <  1:643875/136‑1 (MQ=255) cTTCAAAAATAACTTCGTACTGGAAGCGGCATTTGGTCAGGCGGAAGGGTATATCGATCAATATTTTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTGcc                                                                                                                                       >  2:643875/1‑136 (MQ=255)    cAAAAATAACTTCGTACTGGAAGCGGCATTTGGTCAGGCGGAAGGGTATATCGATCAATATTTTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATa                                                                                                                                <  1:650855/140‑1 (MQ=255)    cAAAAATAACTTCGTACTGGAAGCGGCATTTGGTCAGGCGGAAGGGTATATCGATCAATATTTTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATa                                                                                                                                >  2:502870/1‑140 (MQ=255)    cAAAAATAACTTCGTACTGGAAGCGGCATTTGGTCAGGCGGAAGGGTATATCGATCAATATTTTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATa                                                                                                                                >  1:24355/1‑140 (MQ=255)    cAAAAATAACTTCGTACTGGAAGCGGCATTTGGTCAGGCGGAAGGGTATATCGATCAATATTTTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATa                                                                                                                                >  1:404759/1‑140 (MQ=255)      aaaaTAACTTCGTACTGGAAGCGGCATTTGGTCAGGCGGAAGGGTATATCGATCAATATTTTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATaaa                                                                                                                              >  2:484807/1‑140 (MQ=255)                cGTACTGGAAGCGGCATTTGGTCAGGCGGAAGGGTATATCGATCAATATTTTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGACGATc                                                                                                                    >  1:153691/1‑140 (MQ=255)                     tGGAAGCGGCATTTGGTCAGGCGGAAGGGTATATCGATCAATACTTTGCCATAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATGAAGTTGACGATCGCAGc                                                                                                               >  1:244664/1‑140 (MQ=255)                          gCGGCATTTGGTCAGGCGGAAGGGTATATCGATCAATATTTTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGa                                                                                                                         <  1:1078923/125‑1 (MQ=255)                          gCGGCATTTGGTCAGGCGGAAGGGTATATCGATCAATATTTTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGa                                                                                                                         >  2:1078923/1‑125 (MQ=255)                           cGGCATTTGGTCAGGCGGAAGGGTATATCGATCAATATTTTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAAc                                                                                                         <  2:404759/140‑1 (MQ=255)                                 ttGGTCAGGCGGAAGGGTATATCGATCAATATTTTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCtt                                                                                                   >  2:195777/1‑140 (MQ=255)                                   ggTCAGGCGGAAGGGTATATCGATCAATATTTTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTa                                                                                                 >  2:350305/1‑140 (MQ=255)                                        ggCGGAAGGGTATATCGATCAATATTTTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACg                                                                                                        >  1:682811/1‑128 (MQ=255)                                        ggCGGAAGGGTATATCGATCAATATTTTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACg                                                                                                        <  2:682811/128‑1 (MQ=255)                                        ggCGGAAGGGTATATCGATCAATATTTTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACg                                                                                            >  1:562383/1‑140 (MQ=255)                                           ggAAGGGTATATCGATCAATATTTTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCa                                                                                         <  2:153691/140‑1 (MQ=255)                                              aGGGTATATCGATCAATATTTTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCg                                                                                      >  2:1102710/1‑140 (MQ=255)                                               gggTATATCGATCAATATTTTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGc                                                                                     <  1:350305/140‑1 (MQ=255)                                                         aTCAATATTTTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCaa                                                                           >  2:1012250/1‑140 (MQ=255)                                                         aTCAATATTTTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCaa                                                                           >  1:497459/1‑140 (MQ=255)                                                          tCAATATTTTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAg                                                                          >  1:820527/1‑140 (MQ=255)                                                          tCAATATTTTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAg                                                                          >  1:826814/1‑140 (MQ=255)                                                          tCAATATTTTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAg                                                                          >  1:1010106/1‑140 (MQ=255)                                                          tCAATATTTTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAg                                                                          >  1:1081324/1‑140 (MQ=255)                                                           cAATATTTTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGc                                                                         >  2:647822/1‑140 (MQ=255)                                                            aaTATTTTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCg                                                                        >  1:860792/1‑140 (MQ=255)                                                              tatTTTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGtt                                                                      <  1:1012250/140‑1 (MQ=255)                                                              tatTTTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGtt                                                                      <  1:1102710/140‑1 (MQ=255)                                                                   tGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCt                                                                 >  1:710513/1‑140 (MQ=255)                                                                     ccAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCttt                                                               >  1:422499/1‑140 (MQ=255)                                                                        aaGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGt                                                            >  1:11451/1‑140 (MQ=255)                                                                        aaGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGt                                                            >  2:50455/1‑140 (MQ=255)                                                                               cTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCggg                                                     >  2:207701/1‑140 (MQ=255)                                                                                      tttGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGa                                              <  2:24355/140‑1 (MQ=255)                                                                                      tttGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGa                                              <  2:422499/140‑1 (MQ=255)                                                                                        tGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACg                                            <  2:738636/140‑1 (MQ=255)                                                                                        tGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACg                                            <  1:310258/140‑1 (MQ=255)                                                                                              cGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTgg                                      >  1:1140427/1‑140 (MQ=255)                                                                                               gCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGa                                     >  2:1171434/1‑140 (MQ=255)                                                                                                ccGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGAt                                    >  2:187014/1‑140 (MQ=255)                                                                                                ccGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTATATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGAt                                    >  2:944705/1‑140 (MQ=255)                                                                                                    tAGCCCGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTgc                                >  1:426957/1‑140 (MQ=255)                                                                                                     aGCCCGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTgcg                               >  1:438728/1‑140 (MQ=255)                                                                                                         cGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTc                           >  2:68269/1‑140 (MQ=255)                                                                                                         cGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTc                           >  1:572207/1‑140 (MQ=255)                                                                                                         cGTTAACCACCAGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTc                           >  2:1052214/1‑140 (MQ=255)                                                                                                             aaCCACCAGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAg                       <  2:629404/140‑1 (MQ=255)                                                                                                              accaccAGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAgg                      <  2:826814/140‑1 (MQ=255)                                                                                                                  ccaGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCAcc                  <  1:195777/140‑1 (MQ=255)                                                                                                                  ccaGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCAcc                  <  2:820527/140‑1 (MQ=255)                                                                                                                  ccaGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCAcc                  <  2:860792/140‑1 (MQ=255)                                                                                                                  ccaGCTACCAGTTCTACGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCAcc                  <  2:1010106/140‑1 (MQ=255)                                                                                                                               cTACGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTg     >  2:482877/1‑140 (MQ=255)                                                                                                                                  cGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACg  <  1:187014/140‑1 (MQ=255)                                                                                                                                  cGGTGCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACg  >  1:495790/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                        CTTCAAAAATAACTTCGTACTGGAAGCGGCATTTGGTCAGGCGGAAGGGTATATCGATCAATATTTTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTACCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACG  >  NC_000913/709020‑709288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑