Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	789,332	A→G	R216R (CGT→CGC)	galK ←	galactokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	789,332	0	A	G	100.0%	175.7 / NA	51	R216R (CGT→CGC)	galK	galactokinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/31);  total (20/31)

CGATCGGGTCCAGTTCATGCGCAACAGCGTTGAACTCTTCAATGGTGACATCACGCAGGGCTGGCTGCTGGAAGAAACGCGCACCGGTTTCGCACTGTTCACGACGGGTGTTGTATTCGCTGCCAACCAGGGTACGTTTGAAGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCT  >  NC_000913/789199‑789456                                                                                                                                      |                                                                                                                             cGATCGGGTCCAGTTCATGCGCAACAGCGTTGAACTCTTCAATGGTGACATCACGCAGGGCTGGCTGCTGGAAGAAACGCGCACCGGTTTCGCACTGTTCACGACGGGTGTTGTATTCGCTGCCAACCAGGGTGCGTTTg                                                                                                                        <  2:555254/140‑1 (MQ=255)    tCGGGTCCAGTTCATGCGCAACAGCGTTGAACTCTTCAATGGTGACATCACGCAGGGCTGGCTGCTGGAAGAAACGCGCACCGGTTTCGCACTGTTCACGACGGGTGTTGTATTCGCTGCCAACCAGGGTGCGTTTGAAg                                                                                                                     <  1:885755/140‑1 (MQ=255)      gggTCCAGTTCATGCGCAACAGCGTTGAACTCTTCAATGGTGACATCACGCAGGGCTGGCTGCTGGAAGAAACGCGCACCGGTTTCGCACTGTTCACGACGGGTGTTGTATTCGCTGCCAACCAGGGTGCGTTTGAAGtt                                                                                                                   <  2:171986/140‑1 (MQ=255)       ggTCCAGTTCATGCGCAACAGCGTTGAACTCTTCAATGGTGACATCACGCAGGGCTGGCTGCTGGAAGAAACGCGCACCGGTTTCGCACTGTTCACGACGGGTGTTGTATTCGCTGCCAACCAGGGTGCGTTTGAAGTTa                                                                                                                  <  1:12202/140‑1 (MQ=255)        gTCCAGTTCATGCGCAACAGCGTTGAACTCTTCAATGGTGACATCACGCAGGGCTGGCTGCTGGAAGAAACGCGCACCGGTTTCGCACTGTTCACGACGGGTGTTGTATTCGCTGCCAACCAGGGTGCGTTTGAAGTTAc                                                                                                                 >  1:520287/1‑140 (MQ=255)        gTCCAGTTCATGCGCAACAGCGTTGAACTCTTCAATGGTGACATCACGCAGGGCTGGCTGCTGGAAGAAACGCGCACCGGTTTCGCACTGTTCACGACGGGTGTTGTATTCGCTGCCAACCAGGGTGCGTTTGAAGTTAc                                                                                                                 <  2:810608/140‑1 (MQ=255)             gTTCATGCGCAACAGCGTTGAACTCTTCAATGGTGACATCACGCAGGGCTGGCTGCTGGAAGAAACGCGCACCGGTTTCGCACTGTTCACGACGGGTGTTGTATTCGCTGCCAACCAGGGTGCGTTTGa                                                                                                                       <  2:1126539/129‑1 (MQ=255)             gTTCATGCGCAACAGCGTTGAACTCTTCAATGGTGACATCACGCAGGGCTGGCTGCTGGAAGAAACGCGCACCGGTTTCGCACTGTTCACGACGGGTGTTGTATTCGCTGCCAACCAGGGTGCGTTTGa                                                                                                                       >  1:1126539/1‑129 (MQ=255)              ttCATGCGCAACAGCGTTGAACTCTTCAATGGTGACATCACGCAGGGCTGGCTGCTGGAAGAAACGCGCACCGGTTTCGCACTGTTCACGACGGGTGTTGTATTCGCTGCCAACCAGGGTGCGTTTGAAGTTACTGTtga                                                                                                           >  1:561796/1‑140 (MQ=255)              ttCATGCGCAACAGCGTTGAACTCTTCAATGGTGACATCACGCAGGGCTGGCTGCTGGAAGAAACGCGCACCGGTTTCGCACTGTTCACGACGGGTGTTGTATTCGCTGCCAACCAGGGTGCGTTTGAAGTTACTGTtga                                                                                                           >  2:196282/1‑140 (MQ=255)              ttCATGCGCAACAGCGTTGAACTCTTCAATGGTGACATCACGCAGGGCTGGCTGCTGGAAGAAACGCGCACCGGTTTCGCACTGTTCACGACGGGTGTTGTATTCGCTGCCAACCAGGGTGCGTTTGAAGTTACTGTtga                                                                                                           >  1:2466/1‑140 (MQ=255)                cATGCGCAACAGCGTTGAACTCTTCAATGGTGACATCACGCAGGGCTGGCTGCTGGAAGAAACGCGCACCGGTTTCGCACTGTTCACGACGGGTGTTGTATTCGCTGCCAACCAGGGTGCGTTTGAAGTTACTGTtgatg                                                                                                         <  1:58196/140‑1 (MQ=255)                  tGCGCAACAGCGTTGAACTCTTCAATGGTGACATCACGCAGGGCTGGCTGCTGGAAGAAACGCGCACCGGTTTCGCACTGTTCACGACGGGTGTTGTATTCGCTGCCAACCAGGGTGCGTTTGAAGTTACTGTtgatgat                                                                                                       >  2:428395/1‑140 (MQ=255)                  tGCGCAACAGCGTTGAACTCTTCAATGGTGACATCACGCAGGGCTGGCTGCTGGAAGAAACGCGCACCGGTTTCGCACTGTTCACGACGGGTGTTGTATTCGCTGCCAACCAGGGTGCGTTTGAAGTTACTGTtgatgat                                                                                                       >  1:76843/1‑140 (MQ=255)                  tGCGCAACAGCGTTGAACTCTTCAATGGTGACATCACGCAGGGCTGGCTGCTGGAAGAAACGCGCACCGGTTTCGCACTGTTCACGACGGGTGTTGTATTCGCTGCCAACCAGGGTGCGTTTGAAGTTACTGTtgatgat                                                                                                       >  2:8/1‑140 (MQ=255)                      cAACAGCGTTGAACTCTTCAATGGTGACATCACGCAGGGCTGGCTGCTGGAAGAAACGCGCACCGGTTTCGCACTGTTCACGACGGGTGTTGTATTCGCTGCCAACCAGGGTGCGTTTGAAGTTACTGTTGATGATgacg                                                                                                   <  2:519744/140‑1 (MQ=255)                      cAACAGCGTTGAACTCTTCAATGGTGACATCACGCAGGGCTGGCTGCTGGAAGAAACGCGCACCGGTTTCGCACTGTTCACGACGGATGTTGTATTCGCTGCCAACCAGGGTGCGTTTGAAGTTACTGTTGATGATgacg                                                                                                   <  2:338278/140‑1 (MQ=255)                        aCAGCGTTGAACTCTTCAATGGTGACATCACGCAGGGCTGGCTGCTGGAAGAAACGCGCACCGGTTTCGCACTGTTCACGACGGGTGTTGTATTCGCTGCCAACCAGGGTGCGTTTGAAGTTa                                                                                                                  <  1:548198/123‑1 (MQ=255)                        aCAGCGTTGAACTCTTCAATGGTGACATCACGCAGGGCTGGCTGCTGGAAGAAACGCGCACCGGTTTCGCACTGTTCACGACGGGTGTTGTATTCGCTGCCAACCAGGGTGCGTTTGAAGTTa                                                                                                                  >  2:548198/1‑123 (MQ=255)                        aCAGCGTTGAACTCTTCAATGGTGACATCACGCAGGGCTGGCTGCTGGAAGAAACGCGCACCGGTTTCGCACTGTTCACGACGGGTGTTGTATTCGCTGCCAACCAGGGTGCGTTTGAAGTTACTGTTGATGATgacgac                                                                                                 >  1:605545/1‑140 (MQ=255)                           gCGTTGAACTCTTCAATGGTGACATCACGCAGGGCTGGCTGCTGGAAGAAACGCGCACCGGTTTCGCACTGTTCACGACGGGTGTTGTATTCGCTGCCAACCAGGGTGCGTTTGAAGTTACTGTTGATGATGACGACAGc                                                                                              <  2:633609/140‑1 (MQ=255)                             gTTGAAACCTTCAATGGTTACATCACTCAGGGCTGGCTGCTGGAAGAAACGCGTACCGGTTTCGCACTGTTCACGAAGGGTGTTGTATTCGCTGCCAACCAGGGTGCGTTTGAAGTTACTGTTGATGATGACGACAGCca                                                                                            <  2:149532/140‑1 (MQ=255)                                  aCTCTTCAATGGTGACATCACGCAGGGCTGGCTGCTGGAAGAAACGCGCACCGGTTTCGCACTGTTCACGACGGGTGTTGTATTCGCTGCCAACCAGGGTGCGTTTGAAGTTACTGTTGATGATGACGACAGCCACACCt                                                                                       <  1:164099/140‑1 (MQ=255)                                    tctTCAATGGTGACATCACGCAGGGCTGGCTGCTGGAAGAAACGCGCACCGGTTTCGCACTGTTCACGACGGGTGTTGTATTCGCTGCCAACCAGGGTGCGTTTGAAGTTACTGTTGATGATGACGACAGCCACACCttt                                                                                     <  1:1061909/140‑1 (MQ=255)                                      ttCAATGGTGACATCACGCAGGGCTGGCTGCTGGAAGAAACGCGCACCGGTTTCGCACTGTTCACGACGGGTGTTGTATTCGCTGCCAACCAGGGTGCGTTTGAAGTTACTGTTGATGATGACGACAGCCACACCTTTgg                                                                                   >  2:438584/1‑140 (MQ=255)                                               gACATCACGCAGGGCTGGCTGCTGGAAGAAACGCGCACCGGTTTCGCACTGTTCACGACGGGTGTTGTATTCGCTGCCAACCAGGGTGCGTTTGAAGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGaaa                                                                          <  2:605545/140‑1 (MQ=255)                                                 cATCACGCAGGGCTGGCTGCTGGAAGAAACGCGCACCGGTTTCGCACTGTTCACGACGGGTGTTGTATTCGCTGCCAACCAGGGTGCGTTTGAAGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACt                                                                        <  2:566350/140‑1 (MQ=255)                                                  aTCACGCAGGGCTGGCTGCTGGAAGAAACGCGCACCGGTTTCGCACTGTTCACGACGGGTGTTGTATTCGCTGCCAACCAGGGTGCGTTTGAAGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTg                                                                       >  2:270365/1‑140 (MQ=255)                                                    cACGCAGGGCTGGCTGCTGGAAGAAACGCGCACCGGTTTCGCACTGTTCACGACGGGTGTTGTATTCGCTGCCAACCAGGGTGCGTTTGAAGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCt                                                                     <  2:1118642/140‑1 (MQ=255)                                                    cACGCAGGGCTGGCTGCTGGAAGAAACGCGCACCGGTTTCGCACTGTTCACGACGGGTGTTGTATTCGCTGCCAACCAGGGTGCGTTTGAAGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCt                                                                     <  1:832024/140‑1 (MQ=255)                                                     aCGCAGGGCTGGCTGCTGGAAGAAACGCGCACCGGTTTCGCACTGTTCACGACGGGTGTTGTATTCGCTGCCAACCAGGGTGCGTTTGAAGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCtt                                                                    >  1:344065/1‑140 (MQ=255)                                                       gCAGGGCTGGCTGCTGGAAGAAACGCGCACCGGTTTCGCACTGTTCACGACGGGTGTTGTATTCGCTGCCAACCAGGGTGCGTTTGAAGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTg                                                                  >  2:1120382/1‑140 (MQ=255)                                                         aGGGCTGGCTGCTGGAAGAAACGCGCACCGGTTTCGCACTGTTCACGACGGGTGTTGTATTCGCTGCCAACCAGGGTGCGTTTGAAGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGt                                                                <  1:1120382/140‑1 (MQ=255)                                                                   gctgGAAGAAACGCGCACCGGTTTCGCACTGTTCACGACGGGTGTTGTATTCGCTGCCAACCAGGGTGCGTTTGAAGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAg                                                      <  2:2466/140‑1 (MQ=255)                                                                    ctgGAAGAAACGCGCACCGGTTTCGCACTGTTCACGACGGGTGTTGTATTCGCTGCCAACCAGGGTGCGTTTGAAGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGc                                                     <  1:438584/140‑1 (MQ=255)                                                                     tgGAAGAAACGCGCACCGGTTTCGCACTGTTCACGACGGGTGTTGTATTCGCTGCCAACCAGGGTGCGTTTGAAGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCg                                                    >  1:469973/1‑140 (MQ=255)                                                                       gaagaaACGCGCACCGGTTTCGCACTGTTCACGACGGGTGTTGTATTCGCTGCCAACCAGGGTGCGTTTGAAGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGc                                                  <  1:8/140‑1 (MQ=255)                                                                             aCGCGCACCGGTTTCGCACTGTTCACGACGGGTGTTGTATTCGCTGCCAACCAGGGTGCGTTTGAAGTTACTGTTGATGATGATGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGa                                            <  1:848866/140‑1 (MQ=255)                                                                                      ggTTTCGCACTGTTCACGACGGGTGTTGTATTCGCTGCCAACCAGGGTGCGTTTGAAGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAgg                                   <  2:561796/140‑1 (MQ=255)                                                                                       gTTTCGCACTGTTCACGACGGGTGTTGTATTCGCTGCCAACCAGGGTGCGTTTGAAGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGc                                  <  2:76843/140‑1 (MQ=255)                                                                                                  ttCACGACGGGTGTTGTATTCGCTGCCAACCAGGGTGCGTTTGAAGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGAtctttct                       >  2:389589/1‑140 (MQ=255)                                                                                                      cgacgGGTGTTGTATTCGCTGCCAACCAGGGTGCGTTTGAAGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGcc                   <  1:428395/140‑1 (MQ=255)                                                                                                           ggTGTTGTATTCGCTGCCAACCAGGGTGCGTTTGAAGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAgcg              <  2:344065/140‑1 (MQ=255)                                                                                                            gtgtTGTATTCGCTGCCAACCAGGGTGCGTTTGAAGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAgcgc             >  1:1030999/1‑140 (MQ=255)                                                                                                            gtgtTGTATTCGCTGCCAACCAGGGTGCGTTTGAAGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAgcgc             >  2:1010251/1‑140 (MQ=255)                                                                                                                      cGCTGCCAACCAGGGTGCGTTTGAAGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGc   <  1:766805/140‑1 (MQ=255)                                                                                                                      cGCTGCCAACCAGGGTGCGTTTGAAGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGc   <  1:449437/140‑1 (MQ=255)                                                                                                                      cGCTGCCAACCAGGGTGCGTTTGAAGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGc   <  2:469973/140‑1 (MQ=255)                                                                                                                       gCTGCCAACCAGGGTGCGTTTGAAGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCt  >  2:530660/1‑140 (MQ=255)                                                                                                                                  gggTGCGTTTGAAGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGc   >  2:578647/1‑128 (MQ=255)                                                                                                                                  gggTGCGTTTGAAGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGc   <  1:578647/128‑1 (MQ=255)                                                                                                                                      |                                                                                                                             CGATCGGGTCCAGTTCATGCGCAACAGCGTTGAACTCTTCAATGGTGACATCACGCAGGGCTGGCTGCTGGAAGAAACGCGCACCGGTTTCGCACTGTTCACGACGGGTGTTGTATTCGCTGCCAACCAGGGTACGTTTGAAGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCT  >  NC_000913/789199‑789456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑