Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	804,031	A→G	T177A (ACC→GCC)	ybhJ →	putative hydratase YbhJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	804,031	0	A	G	100.0%	210.3 / NA	60	T177A (ACC→GCC)	ybhJ	putative hydratase YbhJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/24);  total (36/24)

TCAATATATGCGTGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACACCCGTTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACACCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAAC  >  NC_000913/803895‑804159                                                                                                                                         |                                                                                                                                 tcAATATATGCGTGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACACCCGTTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCt                                                                                                                               <  1:672459/140‑1 (MQ=255)    atatatGCGTGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACACCCGTTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTggg                                                                                                                            >  2:1072474/1‑140 (MQ=255)        atGCGTGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACACCCGTTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGatat                                                                                                                        >  2:974485/1‑140 (MQ=255)        atGCGTGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACACCCGTTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGatat                                                                                                                        >  2:220382/1‑140 (MQ=255)          gCGTGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACACCCGTTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCg                                                                                                                      >  2:576349/1‑140 (MQ=255)           cGTGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACACCCGTTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGa                                                                                                                     <  2:269849/140‑1 (MQ=255)            gTGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACACCCGTTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGAc                                                                                                                    >  2:696010/1‑140 (MQ=255)               agaTGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACACCCGTTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTAt                                                                                                                 >  2:984123/1‑140 (MQ=255)               agaTGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACACCCGTTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTAt                                                                                                                 >  1:268094/1‑140 (MQ=255)               agaTGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACACCCGTTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTAt                                                                                                                 >  1:284350/1‑140 (MQ=255)               agaTGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACACCCGTTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTAt                                                                                                                 >  1:292110/1‑140 (MQ=255)               agaTGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACACCCGTTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTAt                                                                                                                 >  1:859546/1‑140 (MQ=255)                 atgatgGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACACCCGTTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTATcc                                                                                                               >  2:478485/1‑140 (MQ=255)                      ggCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACGCCCGTTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTATCCGGGCg                                                                                                          >  1:105448/1‑140 (MQ=255)                      ggCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACACCCGTTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTATCCGGGCg                                                                                                          >  2:279452/1‑140 (MQ=255)                          ggcggcggcAAAATGATCCTCGGGTCAGACAGCCACACCCGTTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTATCCGGGCGTGGt                                                                                                      >  2:948425/1‑140 (MQ=255)                          ggcggcggcAAAATGATCCTCGGGTCAGACAGCCACACCCGTTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTATCCGGGCGTGGt                                                                                                      >  2:785996/1‑140 (MQ=255)                                         aTCCTCGGGTCAGACAGCCACACCCGTTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGAc                                                                                       <  1:68395/140‑1 (MQ=255)                                              cGGGTCAGACAGCCACACCCGTTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGaa                                                                                  >  2:489098/1‑140 (MQ=255)                                                 gTCAGACAGCCACACCCGTTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAAc                                                                               >  1:716980/1‑140 (MQ=255)                                                   cAGACAGCCACACCCGTTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCt                                                                             <  1:279452/140‑1 (MQ=255)                                                    aGACAGCCACACCCGTTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTATCCAGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTg                                                                            <  2:1094611/140‑1 (MQ=255)                                                      aCAGCCACACCCGTTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTgcg                                                                          <  1:592667/140‑1 (MQ=255)                                                      aCAGCCACACCCGTTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTgcg                                                                          <  1:503767/140‑1 (MQ=255)                                                         gCCACACCCGTTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCg                                                                       <  1:622123/140‑1 (MQ=255)                                                           cacaCCCGTTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAAc                                                                               >  2:535837/1‑130 (MQ=255)                                                           cacaCCCGTTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAAc                                                                               <  1:535837/130‑1 (MQ=255)                                                             cacCCGTTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTAtg                                                                   <  1:961708/140‑1 (MQ=255)                                                                  gTTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTgggg                                                              <  1:974485/140‑1 (MQ=255)                                                                      cgctGCATTAGGGACAATGGCAGTCGGTGACGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCcac                                                          <  2:716980/137‑1 (MQ=255)                                                                       ggTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCcaca                                                         <  2:268094/140‑1 (MQ=255)                                                                        gTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAg                                                        >  1:426288/1‑140 (MQ=255)                                                                                 ggACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGgcg                                               <  2:426288/140‑1 (MQ=255)                                                                                   aCAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCt                                             >  1:1016313/1‑140 (MQ=255)                                                                                     aaTGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTgg                                           >  2:57970/1‑140 (MQ=255)                                                                                     aaTGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTgg                                           >  1:757391/1‑140 (MQ=255)                                                                                     aaTGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTgg                                           >  2:57875/1‑140 (MQ=255)                                                                                       tGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCt                                         >  2:975365/1‑140 (MQ=255)                                                                                       tGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCt                                         >  1:891007/1‑140 (MQ=255)                                                                                        ggCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGc                                          >  2:594126/1‑138 (MQ=255)                                                                                        ggCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGc                                          <  1:594126/138‑1 (MQ=255)                                                                                            gTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCAt                                    <  1:785996/140‑1 (MQ=255)                                                                                             tCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCAtt                                   >  1:674437/1‑140 (MQ=255)                                                                                             tCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCAtt                                   >  2:262526/1‑140 (MQ=255)                                                                                               ggTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTgg                                 >  1:953705/1‑140 (MQ=255)                                                                                                 tGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGgcg                               <  1:1072474/140‑1 (MQ=255)                                                                                                 tGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGgcg                               <  1:475238/140‑1 (MQ=255)                                                                                                 tGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGgcg                               <  1:984123/140‑1 (MQ=255)                                                                                                 tGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGgcg                               <  1:489098/140‑1 (MQ=255)                                                                                                    gggCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCgg                            <  1:872233/140‑1 (MQ=255)                                                                                                    gggCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCgg                            <  1:576349/140‑1 (MQ=255)                                                                                                      gcggcgGTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGgtg                          <  2:292110/140‑1 (MQ=255)                                                                                                         gcggTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTc                       >  1:152260/1‑140 (MQ=255)                                                                                                          cggTGAGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCACCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCa                      >  1:989406/1‑140 (MQ=255)                                                                                                               aGTTGGTAAAACAGCTGCTTAATGACGCCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAAc                 >  1:633344/1‑140 (MQ=255)                                                                                                                           aGCTGCTTAATGACGCCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCaaa     >  1:855532/1‑140 (MQ=255)                                                                                                                             ctgctTAATGACGCCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCaaaaa   <  2:105448/140‑1 (MQ=255)                                                                                                                             ctgctTAATGACGCCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCaaaaa   >  1:796470/1‑140 (MQ=255)                                                                                                                             ctgctTAATGACGCCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATATGGGGCCACAGGATGTGGCGCTGGCTATCAATGGCGCGGTGTTCAAAAACGGTTACGTCaaaaa   >  2:933401/1‑140 (MQ=255)                                                                                                                              tgctTAATGACGCCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAAc  >  1:509061/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                 TCAATATATGCGTGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACACCCGTTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACACCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAAC  >  NC_000913/803895‑804159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑