Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	945,476	C→T	V28I (GTA→ATA)	ycaC ←	putative hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	945,476	0	C	T	100.0%	197.2 / NA	55	V28I (GTA→ATA)	ycaC	putative hydrolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/28);  total (27/28)

TCAGTTCTGGAACTAATGGGCCGTTAGGACCGGTTTCAAAACTGGTGGTAAGAATAGTTGGTAAGTTGAAATACTTAGCCAGGTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTACAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGTCAGTGGTACGTCTGAAATCGATCAGACGCGAT  >  NC_000913/945341‑945609                                                                                                                                        |                                                                                                                                      tCAGTTCTGGAACTAATGGGCCGTTAGGACCGGTTTCAAAACTGGTGGTAAGAATAGTTGGTAAGTTGAAATACTTAGCCAGGTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATAAgg                                                                                                                                   <  1:1117155/140‑1 (MQ=255)                aTGGGCCGTTAGGACCGGTTTCAAAACTGGTGGTAAGAATAGTTGGTAAGTTGAAATACTTAGCCAGGTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGcc                                                                                                                    <  1:189693/140‑1 (MQ=255)                 tGGGCCGTTAGGACCGGTTTCAAAACTGGTGGTAAGAATAGTTGGTAAGTTGAAATACTTAGCCAGGTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCt                                                                                                                   >  1:678171/1‑140 (MQ=255)                    gCCGTTAGGACCGGTTTCAAAACTGGTGGTAAGAATAGTTGGTAAGTTGAAATACTTAGCCAGGTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGt                                                                                                                <  2:1052195/140‑1 (MQ=255)                          aGGACCGGTTTCAAAACTGGTGGTAAGAATAGTTGGTAAGTTGAAATACTTAGCCAGGTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTGATcaa                                                                                                          <  1:170735/140‑1 (MQ=255)                                ggTTTCAAAACTGGTGGTAAGAATAGTTGGTAAGTTGAAATACTTAGCCAGGTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGt                                                                                                                <  2:987937/128‑1 (MQ=255)                                ggTTTCAAAACTGGTGGTAAGAATAGTTGGTAAGTTGAAATACTTAGCCAGGTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGt                                                                                                                >  1:987937/1‑128 (MQ=255)                                  tttCAAAACTGGTGGTAAGAATAGTTGGTAAGTTGAAATACTTAGCCAGGTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTg                                                                                                               >  2:187913/1‑127 (MQ=255)                                  tttCAAAACTGGTGGTAAGAATAGTTGGTAAGTTGAAATACTTAGCCAGGTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTg                                                                                                               <  1:187913/127‑1 (MQ=255)                                  tttCAAAACTGGTGGTAAGAATAGTTGGTAAGTTGAAATACTTAGCCAGGTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCaaa                                                                                                  >  1:838863/1‑140 (MQ=255)                                     cAAAACTGGTGGTAAGAATAGTTGGTAAGTTGAAATACTTAGCCAGGTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACg                                                                                               <  1:677417/140‑1 (MQ=255)                                      aaaaCTGGTGGTAAGAATAGTTGGTAAGTTGAAATACTTAGCCAGGTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACgg                                                                                              <  2:716583/140‑1 (MQ=255)                                                        aGTTGGTAAGTTGAAATACTTAGCCAGGTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCaa                                                                            >  2:679102/1‑140 (MQ=255)                                                          ttGGTAAGTTGAAATACTTAGCCAGGTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAATCCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGa                                                                          >  2:280420/1‑140 (MQ=255)                                                           tGGTAAGTTGAAATACTTAGCCAGGTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAATCCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGAc                                                                         <  1:280420/140‑1 (MQ=255)                                                            ggTAAGTTGAAATACTTAGCCAGGTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACg                                                                        >  2:373081/1‑140 (MQ=255)                                                            ggTAAGTTGAAATACTTAGCCAGGTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACg                                                                        >  2:114110/1‑140 (MQ=255)                                                               aaGTTGAAATACTTAGCCAGGTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACGAAc                                                                     >  1:325225/1‑140 (MQ=255)                                                                aGTTGAAATACTTAGCCAGGTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACa                                                                    >  1:1005515/1‑140 (MQ=255)                                                                  ttGAAATACTTAGCCAGGTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATa                                                                  <  2:213118/140‑1 (MQ=255)                                                                     aaaTACTTAGCCAGGTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACgg                                                               <  1:336888/140‑1 (MQ=255)                                                                            tAGCCAGGTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATACGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCCACATCATTTTTATCAAGACGAACATACGGTTTGGTc                                                        >  2:861594/1‑140 (MQ=255)                                                                            tAGCCAGGTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTc                                                        >  2:710201/1‑140 (MQ=255)                                                                            tAGCCAGGTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTc                                                        >  1:177753/1‑140 (MQ=255)                                                                               ccAGGTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATc                                                     >  1:715578/1‑140 (MQ=255)                                                                                  ggTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCctc                                                  >  2:242097/1‑140 (MQ=255)                                                                                   gTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTgg                                                          <  1:942710/131‑1 (MQ=255)                                                                                   gTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTgg                                                          >  2:942710/1‑131 (MQ=255)                                                                                   gTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCctct                                                 >  1:936105/1‑140 (MQ=255)                                                                                    tCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCa                                                       <  1:245003/133‑1 (MQ=255)                                                                                    tCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCa                                                       >  2:245003/1‑133 (MQ=255)                                                                                    tCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCtctt                                                >  1:134601/1‑140 (MQ=255)                                                                                       cccAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCa                                                       <  1:328919/130‑1 (MQ=255)                                                                                       cccAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCa                                                       >  2:328919/1‑130 (MQ=255)                                                                                       cccAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTtctc                                             >  2:141520/1‑140 (MQ=255)                                                                                       cccAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTtctc                                             >  2:158715/1‑140 (MQ=255)                                                                                        ccAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTcc                                            >  1:213921/1‑140 (MQ=255)                                                                                            cgcCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTc                                        >  1:1052869/1‑140 (MQ=255)                                                                                            cgcCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTc                                        >  2:469768/1‑140 (MQ=255)                                                                                              ccAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCga                                      <  1:688184/140‑1 (MQ=255)                                                                                               cAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCgag                                     <  1:158715/140‑1 (MQ=255)                                                                                               cAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCgag                                     <  1:141520/140‑1 (MQ=255)                                                                                                  cACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGt                                  <  1:296272/140‑1 (MQ=255)                                                                                                  cACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGt                                  >  2:468299/1‑140 (MQ=255)                                                                                                   aCGTTATTTTTAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGTc                                 <  2:244829/140‑1 (MQ=255)                                                                                                         tttttAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGTCAGTGGt                           <  2:134601/140‑1 (MQ=255)                                                                                                         tttttAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGTCAGTGGt                           <  1:242097/140‑1 (MQ=255)                                                                                                         tttttAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGTCAGTGGt                           <  1:373081/140‑1 (MQ=255)                                                                                                            ttAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGTCAGTGGTACg                        <  2:807171/140‑1 (MQ=255)                                                                                                             tAAACTTATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGTCAGTGGTACGt                       <  2:1005515/140‑1 (MQ=255)                                                                                                                   tATCGGGTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGTCAGTGGTACGTCTGaaa                 >  1:1007303/1‑140 (MQ=255)                                                                                                                        ggTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGTCAGTGGTACGTCTGAAATCGAt            <  1:1117106/140‑1 (MQ=255)                                                                                                                        ggTTCGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGTCAGTGGTACGTCTGAAATCGAt            <  1:679102/140‑1 (MQ=255)                                                                                                                            cGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGTCAGTGGTACGTCTGAAATCGATCAg         <  2:316338/139‑1 (MQ=255)                                                                                                                            cGATATCCCGTATAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGTCAGTGGTACGTCTGAAATCGATCAg         >  1:316338/1‑139 (MQ=255)                                                                                                                                  cccGTATAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGTCAGTGGTACGTCTGAAATCGATCAGACGCGAt  <  1:114110/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                      TCAGTTCTGGAACTAATGGGCCGTTAGGACCGGTTTCAAAACTGGTGGTAAGAATAGTTGGTAAGTTGAAATACTTAGCCAGGTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTACAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGTCAGTGGTACGTCTGAAATCGATCAGACGCGAT  >  NC_000913/945341‑945609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑