BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	55,228	A→G	Y628H (TAC→CAC)	lptD ←	lipopolysaccharide assembly protein LptD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	55,228	0	A	G	100.0%	405.5 / NA	113	Y628H (TAC→CAC)	lptD	lipopolysaccharide assembly protein LptD
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (53/60); total (53/60)

Only 100 of 115 total aligned reads displayed.

GAATAGTACTTAGGCAGCGTAGCCTGAATATATTCCGGGCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTACTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCCACACCAGTGAACCCGTTTTGTCGTCATTCTCCCATGTTATGTTGTCATCGCCAGTGCGAGACTCCGTGAAATAGTAG  >  NC_000913/55092‑55364

gAATAGTACTTAGGCAGCGTAGCCTGAATATATTCCGGGCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTg                                                                                                                                       >  1:1063499/1‑140 (MQ=255)

  aTAGTACTTAGGCAGCGTAGCCTGAATATATTCCGGGCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGaa                                                                                                                                     <  2:749247/140‑1 (MQ=255)

   tAGTACTTAGGCAGCGTAGCCTGAATATATTCCGGGCTGGCGTAACGGTAATTCAGCTATACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAAt                                                                                                                                    <  1:665317/140‑1 (MQ=255)

     gTACTTAGGCAGCGTAGCCTGAATATATTCCGGGCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATcc                                                                                                                                  >  2:952355/1‑140 (MQ=255)

     gTACTTAGGCAGCGTAGCCTGAATATATTCCGGGCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATcc                                                                                                                                  >  1:403320/1‑140 (MQ=255)

     gTACTTAGGCAGCGTAGCCTGAATATATTCCGGGCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATcc                                                                                                                                  >  1:1142608/1‑140 (MQ=255)

     gTACTTAGGCAGCGTAGCCTGAATATATTCCGGGCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATcc                                                                                                                                  >  1:130342/1‑140 (MQ=255)

      tACTTAGGCAGCGTAGCCTGAATATATTCCGGGCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATccc                                                                                                                                 >  2:1167326/1‑140 (MQ=255)

      tACTTAGGCAGCGTAGCCTGAATATATTCCGGGCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATccc                                                                                                                                 >  1:408726/1‑140 (MQ=255)

      tACTTAGGCAACGTAGCCTGAATATATTCCGGGCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATccc                                                                                                                                 >  2:384123/1‑140 (MQ=255)

       aCTTAGGCAGCGTAGCCTGAATATATTCCGGGCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCg                                                                                                                                <  2:1063499/140‑1 (MQ=255)

        cTTAGGCAGCGTAGCCTGAATATATTCCGGGCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGc                                                                                                                               >  1:890781/1‑140 (MQ=255)

        cTTAGGCAGCGTAGCCTGAATATATTCCGGGCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGc                                                                                                                               >  2:407058/1‑140 (MQ=255)

        cTTAGGCAGCGTAGCCTGAATATATTCCGGGCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGc                                                                                                                               >  1:698789/1‑140 (MQ=255)

        cTTAGGCAGCGTAGCCTGAATATATTCCGGGCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGc                                                                                                                               >  2:943331/1‑140 (MQ=255)

        cTTAGGCAGCGTAGCCTGAATATATTCCGGGCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGc                                                                                                                               >  2:163272/1‑140 (MQ=255)

         ttAGGCAGCGTAGCCTGAATATATTCCGGGCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGcc                                                                                                                              <  2:890781/140‑1 (MQ=255)

            ggCAGCGTAGCCTGAATATATTCCGGGCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACg                                                                                                                           <  1:149478/140‑1 (MQ=255)

             gCAGCGTAGCCTGAATATATTCCGGGCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGc                                                                                                                          <  1:9248/140‑1 (MQ=255)

                gCGTAGCCTGAATATATTCCGGGCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAAt                                                                                                                       >  2:1096830/1‑140 (MQ=255)

                 cGTAGCCTGAATATATTCCGGGCTGGCGTAACGGTAATTCAGCTGTACCAGACGACCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATc                                                                                                                      >  1:139185/1‑140 (MQ=255)

                  gTAGCCTGAATATATTCCGGGCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACg                                                                                                                           >  2:780957/1‑134 (MQ=255)

                  gTAGCCTGAATATATTCCGGGCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACg                                                                                                                           <  1:78281/134‑1 (MQ=255)

                  gTAGCCTGAATATATTCCGGGCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACg                                                                                                                           <  1:780957/134‑1 (MQ=255)

                  gTAGCCTGAATATATTCCGGGCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACg                                                                                                                           >  2:78281/1‑134 (MQ=255)

                  gTAGCCTGAATATATTCCGGGCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATcc                                                                                                                     >  2:320261/1‑140 (MQ=255)

                  gTACCCTGAATATATTCCGGGCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGCAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATcc                                                                                                                     >  2:412740/1‑140 (MQ=255)

                    aGCCTGAATATATTCCGGGCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATcccc                                                                                                                   <  1:865427/140‑1 (MQ=255)

                     gCCTGAATATATTCCGGGCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCa                                                                                                                  <  2:580967/140‑1 (MQ=255)

                      ccTGAATATATTCCGGGCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCaa                                                                                                                 <  1:407058/140‑1 (MQ=255)

                      ccTGAATATATTCCGGGCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCaa                                                                                                                 <  1:1097747/140‑1 (MQ=255)

                      ccTGAATATATTCCGGGCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCaa                                                                                                                 <  1:163272/140‑1 (MQ=255)

                       cTGAATATATTCCGGGCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAAc                                                                                                                >  2:711805/1‑140 (MQ=255)

                          aaTATATTCCGGGCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGcc                                                                                                                              >  1:161630/1‑123 (MQ=255)

                          aaTATATTCCGGGCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGcc                                                                                                                              <  2:161630/123‑1 (MQ=255)

                           atatatTCCGGGCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTc                                                                                                            <  1:711805/140‑1 (MQ=255)

                           atatatTCCGGGCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTc                                                                                                            >  2:955201/1‑140 (MQ=255)

                               atTCCGGGCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGgag                                                                                                        <  1:955201/140‑1 (MQ=255)

                                    gggCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCTTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACg                                                                                                   >  2:1007683/1‑140 (MQ=255)

                                    gggCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGgaga                                                                                                       <  1:463963/136‑1 (MQ=255)

                                    gggCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGgaga                                                                                                       >  2:463963/1‑136 (MQ=255)

                                    gggCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGgag                                                                                                        >  1:896932/1‑135 (MQ=255)

                                    gggCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGgag                                                                                                        <  2:896932/135‑1 (MQ=255)

                                    gggCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACg                                                                                                   <  2:403320/140‑1 (MQ=255)

                                    gggCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACg                                                                                                   <  1:373796/140‑1 (MQ=255)

                                      gCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGc                                                                                                  <  1:831731/139‑1 (MQ=255)

                                      gCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGc                                                                                                  >  2:831731/1‑139 (MQ=255)

                                          gCGTAGCGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCagta                                                                                             >  2:366793/1‑140 (MQ=255)

                                           cGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCagtaa                                                                                            <  1:350421/140‑1 (MQ=255)

                                             tAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCagtaagt                                                                                          <  1:539879/140‑1 (MQ=255)

                                                 ggTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCg                                                                                      <  1:320261/140‑1 (MQ=255)

                                                  gTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGc                                                                                     <  2:740638/140‑1 (MQ=255)

                                                  gTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGc                                                                                     <  1:308713/140‑1 (MQ=255)

                                                    aaTTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCt                                                                                   <  2:1059759/140‑1 (MQ=255)

                                                     aTTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTg                                                                                  >  2:328431/1‑140 (MQ=255)

                                                       tCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGcc                                                                                <  2:130342/140‑1 (MQ=255)

                                                       tCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGcc                                                                                <  1:584614/140‑1 (MQ=255)

                                                         aGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCca                                                                              <  1:1166820/140‑1 (MQ=255)

                                                          gCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCcac                                                                             <  1:952355/140‑1 (MQ=255)

                                                                   gACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCCACACCAGTGaa                                                                    >  2:623577/1‑140 (MQ=255)

                                                                        tCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCCACACCAGTGAACCCGt                                                               >  2:25987/1‑140 (MQ=255)

                                                                        tCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCCACACCAGTGAACCCGt                                                               >  2:236230/1‑140 (MQ=255)

                                                                          ttCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCCACACCAGTGAACCCGttt                                                             <  1:943331/140‑1 (MQ=255)

                                                                               cccGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCCACACCAGTGAACCCGTTTTgtcg                                                        <  1:623577/140‑1 (MQ=255)

                                                                                  gACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCCACACCAGTGAACCCGTTTTGTCGTCa                                                     <  1:260218/140‑1 (MQ=255)

                                                                                  gACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCCACACCAGTGAACCCGTTTTGTCGTCa                                                     <  1:236230/140‑1 (MQ=255)

                                                                                    cGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCCACACCAGTGAACCCGTTTTGTCGTCAtt                                                   <  2:342155/140‑1 (MQ=255)

                                                                                      gTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCCACACCAGTGAACCCGTTTTGTCGTCATtct                                                 <  1:328431/140‑1 (MQ=255)

                                                                                       tATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCCACACCAGTGAACCCGTTTTGTCGTCATtctc                                                <  2:56479/140‑1 (MQ=255)

                                                                                         ttCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCCACACCAGTGAACCCGTTTTGTCGTCATTCTccc                                              >  2:314746/1‑140 (MQ=255)

                                                                                            aaTGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCCACACCAGTGAACCCGTTTTGTCGTCATTCTCCCatg                                           <  1:353639/140‑1 (MQ=255)

                                                                                                 tGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCCACACCAGTGACCCCGTTTTGTCGTCATTCTCCCatgttatg                                      <  2:1059131/140‑1 (MQ=255)

                                                                                                 tGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCCACACCAGTGAACCCGTTTTGTCGTCATTCTccc                                              >  2:737847/1‑132 (MQ=255)

                                                                                                 tGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCCACACCAGTGAACCCGTTTTGTCGTCATTCTCCCatgttatg                                      >  2:363918/1‑140 (MQ=255)

                                                                                                 tGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCCACACCAGTGAACCCGTTTTGTCGTCATTCTCCCatgttatg                                      >  1:34129/1‑140 (MQ=255)

                                                                                                 tGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCCACACCAGTGAACCCGTTTTGTCGTCATTCTCCCatgttatg                                      >  1:87837/1‑140 (MQ=255)

                                                                                                 tGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCCACACCAGTGAACCCGTTTTGTCGTCATTCTCCCatgttatg                                      >  1:880491/1‑140 (MQ=255)

                                                                                                 tGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCCACACCAGTGAACCCGTTTTGTCGTCATTCTCCCatgttatg                                      <  1:412740/140‑1 (MQ=255)

                                                                                                   gAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCCACACCAGTGAACCCGTTTTGTCGTCATTCTCCCatgttatgtt                                    >  1:170146/1‑140 (MQ=255)

                                                                                                      ttACTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCCACACCAGTGAACCCGTTTTGTCGTCATTCTCCCATGTTATGTTGTc                                 >  1:1119452/1‑140 (MQ=255)

                                                                                                        aCTGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCCACACCAGTGAACCCGTTTTGTCGTCATTCTCCCATGTTATGTTGTCAt                               >  1:402096/1‑140 (MQ=255)

                                                                                                          tGGTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCCACACCAGTGAACCCGTTTTGTCGTCATTCTCCCATGTTATGTTGTCATCg                             >  2:1076/1‑140 (MQ=255)

                                                                                                           ggTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCCACACCAGTGAACCCGTTTTGTCGTCATTCTCCCATGTTATGTTGTCATCGc                            <  2:408726/140‑1 (MQ=255)

                                                                                                           ggTCGCTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCCACACCAGTGAACCCGTTTTGTCGTCATTCTCCCATGTTATGTTGTCATCGc                            <  1:1076/140‑1 (MQ=255)

                                                                                                                cTACGTTATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCCACACCAGTGAACCCGTTTTGTCGTCATTCTCCCATGTTATGTTGTCATCGCCAGTg                       >  1:550918/1‑140 (MQ=255)

                                                                                                                     ttATCCAGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCCACACCAGTGAACCCGTTTTGTCGTCATTCTCCCATGTTATGTTGTCATCGCCAGTGCgaga                  <  2:550918/140‑1 (MQ=255)

                                                                                                                           aGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCCACACCAGTGAACCCGTTTTGTCGTCATTCTCCCATGTTATGTTGTCATCGCCAGTGCGAGACTCCGt            >  2:88361/1‑140 (MQ=255)

                                                                                                                           aGACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCCACACCAGTGAACCCGTTTTGTCGTCATTCTCCCATGTTATGTTGTCATCGCCAGTGCGAGACTCCGt            >  2:525774/1‑140 (MQ=255)

                                                                                                                            gACGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCCACACCAGTGAACCCGTTTTGTCGTCATTCTCCCATGTTATGTTGTCATCGCCAGTGCGAGACTCCGTg           <  1:302205/140‑1 (MQ=255)

                                                                                                                             aCGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCCACACCAGTGAACCCGTTTTGTCGTCATTCTCCCATGTTATGTTGTCATCGCCAGTGCGAGACTCCGTGa          <  1:366793/140‑1 (MQ=255)

                                                                                                                             aCGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCCACACCAGTGAACCCGTTTTGTCGTCATTCTCCCATGTTATGTTGTCATCGCCAGTGCGAGACTCCGTGa          <  2:1154653/140‑1 (MQ=255)

                                                                                                                             aCGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCCACACCAGTGAACCCGTTTTGTCGTCATTCTCCCATGTTATGTTGTCATCGCCAGTGCGAGACTCCGTGa          <  1:525774/140‑1 (MQ=255)

                                                                                                                             aCGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCCACACCAGTGAACCCGTTTTGTCGTCATTCTCCCATGTTATGTTGTCATCGCCAGTGCGAGACTCCGTGa          <  2:402096/140‑1 (MQ=255)

                                                                                                                             aCGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCCACACCAGTGAACCCGTTTTGTCGTCATTCTCCCATGTTATGTTGTCATCGCCAGTGCGAGACTCCGTGa          >  1:689211/1‑140 (MQ=255)

                                                                                                                             aCGTGTATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCCACACCAGTGAACCCGTTTTGTCGTCATTCTCCCATGTTATGTTGTCATCGCCAGTGCGAGACTCCGTGa          >  1:74718/1‑140 (MQ=255)

                                                                                                                                 gtATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCCACACCAGTGAACCCGTTTTGTCGTCATTCTCCCATGTTATGTTGTCATCGCCAGTGCGAGACTCCGTGAAAta      <  2:689211/140‑1 (MQ=255)

                                                                                                                                  tATCGTGCTGAATCCCGCCATGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCCACACCAGTGAACCCGTTTTGTCGTCATTCTCCCATGTTATGTTGTCATCGCCAGTGCGAGACTCCGTGa          >  2:1075628/1‑135 (MQ=255)

                                                                                                                                  tATCGTGCTGAATCCCGCCATGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCCACACCAGTGAACCCGTTTTGTCGTCATTCTCCCATGTTATGTTGTCATCGCCAGTGCGAGACTCCGTGa          <  1:1075628/135‑1 (MQ=255)

                                                                                                                                  tATCGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCCACACCAGTGAACCCGTTTTGTCGTCATTCTCCCATGTTATGTTGTCATCGCCAGTGCGAGACTCCGTGAAAtag     <  1:384123/140‑1 (MQ=255)

                                                                                                                                     cGTGCTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCCACACCAGTGAACCCGTTTTGTCGTCATTCTCCCATGTTATGTTGTCATCGCCAGTGCGAGACTCCGTGAAAtagtag  >  2:267619/1‑140 (MQ=255)

GAATAGTACTTAGGCAGCGTAGCCTGAATATATTCCGGGCTGGCGTAACGGTAATTCAGCTGTACCAGACGGTCTTCATCCCGACGGTATTCAATGCTGGAGTTACTGGTCGCTACGTTATCCAGACGTGTATCGTACTGAATCCCGCCACGCAATCCCCAACGCTCGGAGATACGCCAGTAAGTATCGCCTGCCCACACCAGTGAACCCGTTTTGTCGTCATTCTCCCATGTTATGTTGTCATCGCCAGTGCGAGACTCCGTGAAATAGTAG  >  NC_000913/55092‑55364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`