Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,194,690	G→T	L88M (CTG→ATG)	rluE ←	23S rRNA pseudouridine(2457) synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,194,690	0	G	T	100.0%	239.0 / NA	67	L88M (CTG→ATG)	rluE	23S rRNA pseudouridine(2457) synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (33/34);  total (33/34)

TCATTTAAGGTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCGGAAGAACATCGTAGGGTTTATTGA  >  NC_000913/1194554‑1194821                                                                                                                                         |                                                                                                                                    tcatTTAAGGTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCAc                                                                                                                                  >  1:1007801/1‑140 (MQ=255) tcatTTAAGGTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCAc                                                                                                                                  >  1:1078664/1‑140 (MQ=255) tcatTTAAGGTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCAc                                                                                                                                  >  2:249130/1‑140 (MQ=255)    tttAAGGTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAg                                                                                                                               <  1:936802/140‑1 (MQ=255)      tAAGGTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAGc                                                                                                                              >  2:527631/1‑139 (MQ=255)      tAAGGTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAGc                                                                                                                              <  1:527631/139‑1 (MQ=255)      tAAGGTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAGCa                                                                                                                             <  1:888007/140‑1 (MQ=255)      tAAGGTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAGCa                                                                                                                             <  1:152346/140‑1 (MQ=255)              cGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAGCaa                                                                                                                            >  1:894785/1‑133 (MQ=255)              cGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAGCaa                                                                                                                            <  2:894785/133‑1 (MQ=255)              cGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCg                                                                                                                     >  2:676274/1‑140 (MQ=255)               gCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGc                                                                                                                    >  2:634656/1‑140 (MQ=255)                  aTTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTAt                                                                                                                 <  1:634656/140‑1 (MQ=255)                    tGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATcg                                                                                                               >  2:75843/1‑140 (MQ=255)                    tGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATcg                                                                                                               >  1:46611/1‑140 (MQ=255)                     gcgcAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATcgc                                                                                                              <  1:1135682/140‑1 (MQ=255)                           ggCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCaa                                                                                                        >  1:749013/1‑140 (MQ=255)                                 aaGTGCGTTTTGTGTGGGAATCCCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGAc                                                                                                  <  2:581669/140‑1 (MQ=255)                                 aaGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGAc                                                                                                  <  1:794750/140‑1 (MQ=255)                                 aaGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGAc                                                                                                  <  2:1007801/140‑1 (MQ=255)                                    tGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACctg                                                                                               <  1:676274/140‑1 (MQ=255)                                      cGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACctgct                                                                                             >  1:1163797/1‑140 (MQ=255)                                                 ggAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACAcc                                                                                  <  2:1054801/140‑1 (MQ=255)                                                  gAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCGGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACAccc                                                                                 >  2:1055315/1‑140 (MQ=255)                                                      aCCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGaa                                                                             >  2:808860/1‑140 (MQ=255)                                                       ccTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAAc                                                                            >  2:898304/1‑140 (MQ=255)                                                       ccTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAAc                                                                            >  2:299028/1‑140 (MQ=255)                                                         ttCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCg                                                                          >  2:977417/1‑140 (MQ=255)                                                         ttCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCg                                                                          >  2:760118/1‑140 (MQ=255)                                                            cACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGa                                                                       >  2:199034/1‑140 (MQ=255)                                                             aCCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGAt                                                                      >  2:466231/1‑140 (MQ=255)                                                                tGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGaa                                                                   <  1:760118/140‑1 (MQ=255)                                                                tGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGaa                                                                   <  1:977417/140‑1 (MQ=255)                                                                 gCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAAt                                                                  >  2:583781/1‑140 (MQ=255)                                                                  cacaTAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAAtt                                                                 >  2:498877/1‑140 (MQ=255)                                                                        ataGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGGCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTa                                                           <  2:46611/140‑1 (MQ=255)                                                                         taGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTaa                                                          >  1:149104/1‑140 (MQ=255)                                                                                 tCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGc                                                  >  2:200492/1‑140 (MQ=255)                                                                                 tCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGc                                                  >  2:275880/1‑140 (MQ=255)                                                                                   cGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGt                                                >  1:387341/1‑140 (MQ=255)                                                                                     gTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTcc                                              <  1:583781/140‑1 (MQ=255)                                                                                                 ggCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGt                                  <  2:1078664/140‑1 (MQ=255)                                                                                                  gCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTg                                 <  1:808860/140‑1 (MQ=255)                                                                                                  gCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTg                                 <  2:588383/140‑1 (MQ=255)                                                                                                  gCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTg                                 <  1:1133208/140‑1 (MQ=255)                                                                                                  gCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTg                                 >  2:133985/1‑140 (MQ=255)                                                                                                   cTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGt                                                >  2:1132934/1‑124 (MQ=255)                                                                                                   cTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGt                                                <  1:1132934/124‑1 (MQ=255)                                                                                                    tGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGaa                               >  1:952692/1‑140 (MQ=255)                                                                                                         tAAACGCGCCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGACGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCg                          >  1:1071394/1‑140 (MQ=255)                                                                                                               cgcCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCGgaagaa                    <  2:749013/140‑1 (MQ=255)                                                                                                               cgcCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCGgaagaa                    <  1:133985/140‑1 (MQ=255)                                                                                                                gcCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCgg                         >  1:389973/1‑134 (MQ=255)                                                                                                                gcCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCgg                         <  2:352579/134‑1 (MQ=255)                                                                                                                gcCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCgg                         <  2:389973/134‑1 (MQ=255)                                                                                                                gcCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCgg                         >  1:352579/1‑134 (MQ=255)                                                                                                                gcCTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCGGAAGAAc                   >  2:627610/1‑140 (MQ=255)                                                                                                                 ccTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCGGAAGAACa                  >  2:929455/1‑140 (MQ=255)                                                                                                                 ccTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCGGAAGAACa                  <  1:249130/140‑1 (MQ=255)                                                                                                                 ccTGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGAGAACTGCGGAAGAACa                  >  2:253934/1‑140 (MQ=255)                                                                                                                   tGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCGGAAGAACATc                <  1:627610/140‑1 (MQ=255)                                                                                                                   tGCAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCGGAAGAACATc                <  2:421172/140‑1 (MQ=255)                                                                                                                     cAACGCGCCGTTATTGGTCATCACCGGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCGGAAGAACATCGt              <  1:1055315/140‑1 (MQ=255)                                                                                                                     cAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCGGAAGAACATCGt              <  2:387341/140‑1 (MQ=255)                                                                                                                     cAACGCGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCGGAAGAACATCGt              <  1:929455/140‑1 (MQ=255)                                                                                                                      aaCGTGCCGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCGGAAGAACATCGTa             <  1:253934/140‑1 (MQ=255)                                                                                                                             cGTTATTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCGGAAGAACATCGTAGGGTTTa      <  1:75843/140‑1 (MQ=255)                                                                                                                                 aTTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCGGAAGAACATCGTAGGGTTTATTGa  <  2:266011/140‑1 (MQ=255)                                                                                                                                 aTTGGTCATCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCGGAAGAACATCGTAGGGTTTATTGa  <  1:466231/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    TCATTTAAGGTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCGGAAGAACATCGTAGGGTTTATTGA  >  NC_000913/1194554‑1194821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑