Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,249,799	A→G	V347A (GTC→GCC)	dhaK ←	dihydroxyacetone kinase subunit K

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,249,799	0	A	G	100.0%	222.6 / NA	63	V347A (GTC→GCC)	dhaK	dihydroxyacetone kinase subunit K
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/32);  total (31/32)

TTCGCGATCCAGTCCGGTAAGATACTCGCTCTCGGTGCTGAAAATATCGCCACAACGAGTGAGCCAGTTAACAATTTGAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGACCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGTGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAA  >  NC_000913/1249662‑1249930                                                                                                                                          |                                                                                                                                    ttCGCGATCCAGTCCGGTAAGATACTCGCTCTCGGTGCTGAAAATATCGCCACAACGAGTGAGCCAGTTAACAATTTGAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGGcc                                                                                                                                   >  1:614011/1‑140 (MQ=255)    gcgATCCAGTCCGGTAAGATACTCGCTCTCGGTGCTGAAAATATCGCCACAACTAGTGAGCCAGTTAACAATTTGAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGGCCggg                                                                                                                                <  1:215803/140‑1 (MQ=255)    gcgATCCAGTCCGGTAAGATACTCGCTCTCGGTGCTGAAAATATCGCCACAACGAGTGAGCCAGTTAACAATTTGAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGGCCggg                                                                                                                                <  1:107664/140‑1 (MQ=255)           aGTCCGGTAAGATACTCGCTCTCGGTGCTGAAAATATCGCCACAACGAGTGAGCCAGTTAACAATTTGAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGGCCGGGGCGTccc                                                                                                                         >  2:181145/1‑140 (MQ=255)            gTCCGGTAAGATACTCGCTCTCGGTGCTGAAAATATCGCCACAACGAGTGAGCCAGTTAACAATTTGAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCa                                                                                                                        >  1:626255/1‑140 (MQ=255)             tCCGGTAAGATACTCGCTCTCGGTGCTGAAAATATCGCCACAACGAGTGAGCCAGTTAACAATTTGAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCag                                                                                                                       >  1:443519/1‑140 (MQ=255)                  tAAGATACTCGCTCTCGGTGCTGAAAATATCGCCACAACGAGTGAGCCAGTTAACAATTTGAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCAGAGTGc                                                                                                                  >  1:753761/1‑140 (MQ=255)                   aaGATACTCGCTCTCGGTGCTGAAAATATCGCCACAACGAGTGAGCCAGTTAACAATTTGAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCAGAGTGcc                                                                                                                 >  2:365194/1‑140 (MQ=255)                       tACTCGCTCTCGGTGCTGAAAATATCGCCACAACGAGTGAGCCAGTTAACAATTTGAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCg                                                                                                             >  1:182260/1‑140 (MQ=255)                         cTCGCTCTCGGTGCTGAAAATATCGCCACAACGAGTGAGCCAGTTAACAATTTGAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGGCCGGGGCGt                                                                                                                            >  2:596971/1‑123 (MQ=255)                         cTCGCTCTCGGTGCTGAAAATATCGCCACAACGAGTGAGCCAGTTAACAATTTGAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGGCCGGGGCGt                                                                                                                            <  1:596971/123‑1 (MQ=255)                         cTCGCTCTCGGTGCTGAAAATATCGCCACAACGAGTGAGCCAGTTAACAATTTGAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGtt                                                                                                           <  1:241067/140‑1 (MQ=255)                          tCGCTCTCGGTGCTGAAAATATCGCCACAACGAGTGAGCCAGTTAACAATTTGAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGttt                                                                                                          >  2:574534/1‑140 (MQ=255)                          tCGCTCTCGGTGCTGAAAATATCGCCACAACGAGAGAAACAGTTAACAATTTGAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGGCCGGCGCGTCCCAGAGGGCCAGCGttt                                                                                                          >  2:233947/1‑140 (MQ=255)                            gCTCTCGGTGCTGAAAATATCGCCACAACGAGTGAGCCAGTTAACAATTTGAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCg                                                                                                        >  2:149885/1‑140 (MQ=255)                              tctcGGTGCTGAAAATATCGCCACAACGAGTGAGCCAGTTAACAATTTGAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGtc                                                                                                      >  2:1164421/1‑140 (MQ=255)                              tctcGGTGCTGAAAATATCGCCACAACGAGTGAGCCAGTTAACAATTTGAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGtc                                                                                                      >  2:398969/1‑140 (MQ=255)                                tcGGTGCTGAAAATATCGCCACAACGAGTGAGCCAGTTAACAATTTGAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCagag                                                                                                                     >  2:1154210/1‑123 (MQ=255)                                tcGGTGCTGAAAATATCGCCACAACGAGTGAGCCAGTTAACAATTTGAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCagag                                                                                                                     <  1:1154210/123‑1 (MQ=255)                                tcGGTGCTGAAAATATCGCCACAACGAGTGAGCCAGTTAACAATTTGAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGtcat                                                                                                    <  2:626255/140‑1 (MQ=255)                                tcGGTGCTGAAAATATCGCCACAACGAGTGAGCCAGTTAACAATTTGAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGtcat                                                                                                    <  1:1164421/140‑1 (MQ=255)                                 cGGTGCTGAAAATATCGCCACAACGAGTGAGCCAGTTAACAATTTGAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGtcatc                                                                                                   <  1:149885/140‑1 (MQ=255)                                    tGCTGAAAATATCGCCACAACGAGTGAGCCAGTTAACAATTTGAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAAc                                                                                                <  2:1011595/140‑1 (MQ=255)                                     gCTGAAAATATCGCCACAACGAGTGAGCCAGTTAACAATTTGAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACt                                                                                               <  1:365194/140‑1 (MQ=255)                                            atatCGCCACAACGAGTGAGCCAGTTAACAATTTGAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTa                                                                                        <  1:250477/140‑1 (MQ=255)                                            atatCGCCACAACGAGTGAGCCAGTTAACAATTTGAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTa                                                                                        <  1:181145/140‑1 (MQ=255)                                              atCGCCACAACGAGTGAGCCAGTTAACAATTTGAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAg                                                                                      >  1:629062/1‑140 (MQ=255)                                                cGCCACAACGAGTGAGCCAGTTAACAATTTGAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGt                                                                                    <  2:443519/140‑1 (MQ=255)                                                cGCCACAACGAGTGAGCCAGTTAACAATTTGAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGt                                                                                    <  2:753761/140‑1 (MQ=255)                                                   cacaACGAGTGAGCCAGTTAACAATTTGAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGAt                                                                                 >  2:789007/1‑140 (MQ=255)                                                           gTGAGCCAGTTAACAATTTGAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAAc                                                                         <  2:408938/140‑1 (MQ=255)                                                           gTGAGCCAGTTAACAATTTGAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAAc                                                                         <  1:77565/140‑1 (MQ=255)                                                           gTGAGCCAGTTAACAATTTGAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAAc                                                                         <  2:161295/140‑1 (MQ=255)                                                           gTGAGCCAGTTAACAATTTGAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGAGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAAc                                                                         <  2:983167/140‑1 (MQ=255)                                                               gCCAGTTAACAATTTGAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGt                                                                     >  2:1110467/1‑140 (MQ=255)                                                                ccAGTTAACAATTTGAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTc                                                                    <  2:614011/140‑1 (MQ=255)                                                                ccAGTTAACAATTTGAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTc                                                                    <  2:420561/140‑1 (MQ=255)                                                                        cAATTTGAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAg                                                            <  2:629062/140‑1 (MQ=255)                                                                          aTTTGAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGTg                                                          <  1:1073163/140‑1 (MQ=255)                                                                              gAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAGGGTGATTGAGAAACCGGTCata                                                                 <  1:223385/129‑1 (MQ=255)                                                                              gAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAGGGTGATTGAGAAACCGGTCata                                                                 >  2:223385/1‑129 (MQ=255)                                                                               aGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGTGAGGTg                                                     <  1:1110467/140‑1 (MQ=255)                                                                                        cAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGG‑CGGGGTTTGGGGCCGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGTGAGGTGCAGTACgcgc                                           <  1:575209/140‑1 (MQ=255)                                                                                           tGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGTGAGGTGCAGTACGCGCCa                                         >  2:561284/1‑140 (MQ=255)                                                                                              cATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGTGAGGTGCAGTACGCGCCaatt                                      >  2:675260/1‑140 (MQ=255)                                                                                                        tcCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGTGAGGTGCAGTACGCGCCAATTAAATTACGtt                            >  2:167639/1‑140 (MQ=255)                                                                                                                  ccccAGTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGTGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCaa                  >  1:277305/1‑140 (MQ=255)                                                                                                                   cccAGTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGTGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAAt                 >  1:482827/1‑140 (MQ=255)                                                                                                                   cccAGTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGTGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAAt                 >  2:815753/1‑140 (MQ=255)                                                                                                                    ccAGTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGTGAGGTGCAGTACGCGCCAATTAAATTACGTTCg                          <  2:402636/130‑1 (MQ=255)                                                                                                                    ccAGTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGTGAGGTGCAGTACGCGCCAATTAAATTACGTTCg                          >  1:402636/1‑130 (MQ=255)                                                                                                                      aGTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGTGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATccc              >  1:669214/1‑140 (MQ=255)                                                                                                                       gTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGTGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCg             >  1:791121/1‑140 (MQ=255)                                                                                                                       gTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGTGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCg             >  1:733086/1‑140 (MQ=255)                                                                                                                       gTTAAGGGCCGGGGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGTGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCg             >  2:634071/1‑140 (MQ=255)                                                                                                                           aGGGCCGGGGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGTGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTg         <  1:789007/140‑1 (MQ=255)                                                                                                                           aGGGCCGGGGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGTGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTg         <  1:675260/140‑1 (MQ=255)                                                                                                                                cGGGGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGTGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGc    <  2:669214/140‑1 (MQ=255)                                                                                                                                cGGGGTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGTGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGc    <  1:263333/140‑1 (MQ=255)                                                                                                                                 ggggTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGTGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTgg     <  1:1122100/138‑1 (MQ=255)                                                                                                                                 ggggTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGTGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTgg     >  2:1122100/1‑138 (MQ=255)                                                                                                                                 ggggTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGTGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCa   <  2:733086/140‑1 (MQ=255)                                                                                                                                  gggTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGTGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCaa  >  2:1136075/1‑140 (MQ=255)                                                                                                                                  gggTGTGGGCCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGTGAGGTGCAGTACGCGCCAATTAAATTACGAACGATAGTCAATCCCGCTTGCTGGCaa  >  2:816363/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                    TTCGCGATCCAGTCCGGTAAGATACTCGCTCTCGGTGCTGAAAATATCGCCACAACGAGTGAGCCAGTTAACAATTTGAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGACCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGTGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAA  >  NC_000913/1249662‑1249930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑