Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,296,375 A→G F583L (TTC→CTC)  adhE ← aldehyde‑alcohol dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,296,3750AG95.2% 133.7 / ‑2.0 42F583L (TTC→CTC) adhEaldehyde‑alcohol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (16/24);  total (17/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCAGCGCATAGTCTGCCAGCGGATATTTCTGACCAGTAGCGTCGTCAGTTACAACCGCAAACGGAGTGACTTCAGAACCTGTACCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGTACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTG  >  NC_000913/1296239‑1296512
                                                                                                                                        |                                                                                                                                         
gTCAGCGCATAGTCTGCCAGCGGATATTTCTGACCAGTAGCGTCGTCAGTTACAACCGCAAACGGAGTGACTTCAGAACCTGTACCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAGCtt                                                                                                                                        <  1:432477/140‑1 (MQ=255)
    gcgcATAGTCTGCCGGCGGATATTTCTGACCAGTAGCGTCGTCAGTTACAACCGCAAACGGAGTGACTTCAGAACCTGTACCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAGCTTGTAg                                                                                                                                    <  2:740941/140‑1 (MQ=255)
     cgcATAGTCTGCCAGCGGATATTTCTGACCAGTAGCGTCGTCAGTTACAACCGCAAACGGAGTGACTTCAGAACCTGTACCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAGCTTGTAGa                                                                                                                                   >  1:294741/1‑140 (MQ=255)
     cgcATAGTCTGCCAGCGGATATTTCTGACCAGTAGCGTCGTCAGTTACAACCGCAAACGGAGTGACTTCAGAACCTGTACCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAGCTTGTAGa                                                                                                                                   <  1:692028/140‑1 (MQ=255)
     cacATAGTCTGCCAGCGGATATTTCTGACCAGTAGCGTCGTCAGTTACAACCGCAAACGGAGTGACTTCAGAACCTGTACCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAGCTTGTAGa                                                                                                                                   <  2:314175/138‑1 (MQ=255)
      gcATAGTCTGCCAGCGGATATTTCTGACCAGTAGCGTCGTCAGTTACAACCGCAAACGGAGTGACTTCAGAACCTGTACCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAGCTTGTAGAt                                                                                                                                  <  1:963515/140‑1 (MQ=255)
             cTGCCAGCGGATATTTCTGACCAGTAGCGTCGTCAGTTACAACCGCAAACGGAGTGACTTCAGAACCTGTACCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAGCTTGTAGATACGTTTa                                                                                                                           <  2:294741/140‑1 (MQ=255)
              tGCCAGCGGATATTTCTGACCAGTAGCGTCGTCAGTTACAACCGCAAACGGAGTGACTTCAGAACCTGTACCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAGCTTGTAGATACGTTTAc                                                                                                                          >  2:557618/1‑140 (MQ=255)
               gCCAGCGGATATTTCTGACCAGTAGCGTCGTCAGTTACAACCGCAAACGGAGTGACTTCAGAACCTGTACCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAGCTTGTAGATACGTTTACg                                                                                                                         <  1:963078/140‑1 (MQ=255)
                    cGGATATTTCTGACCAGTAGCGTCGTCAGTTACAACCGCAAACGGAGTGACTTCAGAACCTGTACCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAGCTTGTa                                                                                                                                     >  2:513241/1‑123 (MQ=255)
                    cGGATATTTCTGACCAGTAGCGTCGTCAGTTACAACCGCAAACGGAGTGACTTCAGAACCTGTACCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAGCTTGTa                                                                                                                                     <  1:513241/123‑1 (MQ=255)
                    cGGATATTTCTGACCAGTAGCGTCGTCAGTTACAACCGCAAACGGAGTGACTTCAGAACCTGTACCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAGCTTGTAGATACGTTTACGGatat                                                                                                                    <  1:1080953/140‑1 (MQ=255)
                      gATATTTCTGACCAGTAGCGTCGTCAGTTACAACCGCAAACGGAGTGACTTCAGAACCTGTACCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAGCTTGTAGATACGTTTACGGATATcc                                                                                                                  >  1:476948/1‑140 (MQ=255)
                              tGACCAGTAGCGTCGTCAGTTACAACCGCAAACGGAGTGACTTCAGAACCTGTACCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAGCTTGTAGATACGTTTACGGATATCCATAAagcg                                                                                                          <  1:91863/140‑1 (MQ=255)
                                     tAGCGTCGTCAGTTACAACCGCAAACGGAGTGACTTCAGAACCTGTACCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAGCTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGcc                                                                                                   >  2:44084/1‑140 (MQ=255)
                                                 ttACAACCGCAAACGGAGTGACTTCAGAACCTGTACCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAGCTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAg                                                                                       >  1:864679/1‑140 (MQ=255)
                                                           aaaCGGAGTGACTTCAGAACCTGTACCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAGCTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCg                                                                             <  2:490730/140‑1 (MQ=255)
                                                             aCGGAGTGACTTCAGAACCTGTACCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAGCTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGa                                                                           <  1:941344/140‑1 (MQ=255)
                                                                 aGTGACTTCAGAACCTGTACCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAGCTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGtt                                                                       <  2:864679/140‑1 (MQ=255)
                                                                 aGTGACTTCAGAACCTGTACCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAGCTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGtt                                                                       <  2:816246/140‑1 (MQ=255)
                                                                  gTGACTTCAGAACCTGTACCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAGCTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTc                                                                      <  1:238395/140‑1 (MQ=255)
                                                                        tCAGAACCTGTACCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAGCTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGTACAt                                                                <  1:628278/140‑1 (MQ=255)
                                                                               cTGTACCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAGCTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGa                                                                           <  2:2726/122‑1 (MQ=255)
                                                                               cTGTACCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAGCTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGa                                                                           >  1:2726/1‑122 (MQ=255)
                                                                                 gTACCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAGCTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGTACATAACCCACAt                                                       >  1:569057/1‑140 (MQ=255)
                                                                                            ggtggtgACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAGCTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGTACATAACCCACATGATCTTCgcgg                                            >  1:735605/1‑140 (MQ=255)
                                                                                            ggtggtgACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAGCTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGTACATAACCCACATGATCTTCgcgg                                            >  2:254214/1‑140 (MQ=255)
                                                                                            ggtggtgACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAGCTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGTACATAACCCACATGATCTTCgcgg                                            >  1:347471/1‑140 (MQ=255)
                                                                                            ggtggtgACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAGCTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGTACATAACCCACATGATCTTCgcgg                                            <  2:476948/140‑1 (MQ=255)
                                                                                             gtggtgACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGTACATAACCCACATGATCTTCgcggc                                           >  1:103410/1‑140 (MQ=255)
                                                                                              tggtgACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAGCTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGTACATAACCCACATGATCTTCgcggcg                                          <  2:347471/140‑1 (MQ=255)
                                                                                                       cGATCATTTTCGCTTTCACGCCCATTTTCGGGAGCTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGTACATAACCCACATGATCTTCGCGGCGTCCATCggg                                 >  2:627416/1‑140 (MQ=255)
                                                                                                        gATCATTTTCGCTTTCACGCCCATTTTCGGGAGCTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGTACATAACCCACATGATCTTCGCGGCGTCCATCgggg                                >  1:620395/1‑140 (MQ=255)
                                                                                                         aTCATTTTCGCTTTCACGCCCATTTTCGGGAGCTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGTACATAACCCACATGATCTTCGCGGCGTCCATCGGGGa                               >  1:830837/1‑140 (MQ=255)
                                                                                                                tCGCTTTCACGCCCATTTTCGGGAGCTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGTACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAaccacca                        <  2:735605/140‑1 (MQ=255)
                                                                                                                 cGCTTTCACGCCCATTTTCGGGAGCTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGTACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAaccaccac                       >  1:557074/1‑140 (MQ=255)
                                                                                                                 cGCTTTCACGCCCATTTTCGGGAGCTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGTACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAaccaccac                       >  2:959022/1‑140 (MQ=255)
                                                                                                                  gCTTTCACGCCCATTTTCGGGAGCTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGTACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAaccaccacc                      <  1:254214/140‑1 (MQ=255)
                                                                                                                         cGCCCATTTTCGGGAGCTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGTACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGcgcg               <  1:44084/140‑1 (MQ=255)
                                                                                                                              aTTTTCGGGAGCTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGTACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAAt          <  1:557618/140‑1 (MQ=255)
                                                                                                                                    gggAGCTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGTACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTc    <  2:264921/140‑1 (MQ=255)
                                                                                                                                      gAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGTACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTg  <  2:103410/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                         
GTCAGCGCATAGTCTGCCAGCGGATATTTCTGACCAGTAGCGTCGTCAGTTACAACCGCAAACGGAGTGACTTCAGAACCTGTACCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGTACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTG  >  NC_000913/1296239‑1296512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑