Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,334,418	A→G	E188E (GAA→GAG)	cysB →	DNA‑binding transcriptional dual regulator CysB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,334,418	0	A	G	100.0%	185.5 / NA	52	E188E (GAA→GAG)	cysB	DNA‑binding transcriptional dual regulator CysB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/27);  total (25/27)

GATTTCGCTATCGCCACAGAAGCGCTGCATCTGTATGAAGATTTAGTGATGTTACCGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAACTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTAACGCCGCGTATCGTTTTCACGGCAACGGATGCTGACGTCAT  >  NC_000913/1334284‑1334543                                                                                                                                        |                                                                                                                              gATTTCGCTATCGCCACAGAAGCGCTGCATCTGTATGAAGATTTAGTTATGTTACCGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGgc                                                                                                                          <  2:941839/140‑1 (MQ=255) gATTTCGCTATCGCCACAGAAGCGCTGCATCTGTATGAAGATTTAGTGATGTTACCGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGgc                                                                                                                          <  1:1053853/140‑1 (MQ=255) gATTTCGCTATCGCCACAGAAGCGCTGCATCTGCATGAAGATTTAGTGATGTTACCGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGgc                                                                                                                          <  2:969071/140‑1 (MQ=255)            cGCCACAGAAGCGCTGCATCTGTATGAAGATTTAGTGATGTTACCGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGt                                                                                                               <  2:447416/140‑1 (MQ=255)              ccACAGAAGCGCTGCATCTGTATGAAGATTTAGTGATGTTACCGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTg                                                                                                             <  2:1109473/140‑1 (MQ=255)               cacaGAAGCGCTGCATCTGTATGAAGATTTAGTGATGTTACCGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTgg                                                                                                            <  1:1167807/140‑1 (MQ=255)                     aGCGCTGCATCTGTATGAAGATTTAGTGATGTTACCGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTGGTGACat                                                                                                      >  2:480883/1‑140 (MQ=255)                      gcgcTGCATCTGTATGAAGATTTAGTGATGTTACCGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTg                                                                                                             >  1:1127660/1‑132 (MQ=255)                      gcgcTGCATCTGTATGAAGATTTAGTGATGTTACCGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTg                                                                                                             <  2:1127660/132‑1 (MQ=255)                         cTGCATCTGTATGAAGATTTAGTGATGTTACCGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTg                                                                                                             >  1:1051016/1‑129 (MQ=255)                         cTGCATCTGTATGAAGATTTAGTGATGTTACCGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTg                                                                                                             <  2:1051016/129‑1 (MQ=255)                         cTGCATCTGTATGAAGATTTAGTGATGTTACCGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTGGTGACATATAc                                                                                                  >  2:958920/1‑140 (MQ=255)                                 gTATGAAGATTTAGTGATGTTACCGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTGGTGACATATACCTTCGGCt                                                                                          >  2:615844/1‑140 (MQ=255)                                      aaGATTTAGTGATGTTACCGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTAcc                                                                                     >  1:344344/1‑140 (MQ=255)                                        gATTTAGTGATGTTACCGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCgg                                                                                   <  1:615844/140‑1 (MQ=255)                                         aTTTAGTGATGTTACCGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGa                                                                                  >  1:1106575/1‑140 (MQ=255)                                          tttAGTGATGTTACCGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGAc                                                                                 >  2:399666/1‑140 (MQ=255)                                            tAGTGATGTTACCGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGt                                                                               >  2:731983/1‑140 (MQ=255)                                               tGATGTTACCGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCa                                                                            <  2:201672/140‑1 (MQ=255)                                               ggATTTTTCCCTGCTTCCCCTGGAATCCGGCTATTTTAGTCCCCCCGGAATCCCCCGC‑GGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCa                                                                            <  1:525796/139‑1 (MQ=255)                                                  tGTTACCGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGaa                                                                         <  2:325755/140‑1 (MQ=255)                                                   gTTACCGTGCTACCACTGGAATCGGGCTATTGTGGTCACTCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAAc                                                                        >  1:278849/1‑140 (MQ=255)                                                   gTTACCGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCgg                                                                                   >  1:112180/1‑129 (MQ=255)                                                   gTTACCGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCgg                                                                                   <  2:112180/129‑1 (MQ=255)                                                    ttACCGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACt                                                                       >  1:691397/1‑140 (MQ=255)                                                    ttACCGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACt                                                                       <  1:731983/140‑1 (MQ=255)                                                        cGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGAt                                                                   >  2:80252/1‑140 (MQ=255)                                                         gTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATa                                                                  <  2:660964/140‑1 (MQ=255)                                                         gTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATa                                                                  <  2:1106575/140‑1 (MQ=255)                                                         gTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATa                                                                  <  1:480883/140‑1 (MQ=255)                                                         gTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATa                                                                  <  1:747711/140‑1 (MQ=255)                                                            cTACCACTGGAATCGGGCTATTGTAGTCACTCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGTGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTg                                                               >  2:69852/1‑140 (MQ=255)                                                                   tGGAATCGGGCTATTGTAGTCACTCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGTGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTaa                                                        <  1:69852/140‑1 (MQ=255)                                                                     gAATCGGGCTATTGTAGTCACTCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATa                                                                  <  1:659058/128‑1 (MQ=255)                                                                     gAATCGGGCTATTGTAGTCACTCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATa                                                                  >  2:659058/1‑128 (MQ=255)                                                                     gAATCGGGCTATTGTAGTCACTCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATAc                                                                 <  1:331464/129‑1 (MQ=255)                                                                     gAATCGGGCTATTGTAGTCACTCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATAc                                                                 >  2:331464/1‑129 (MQ=255)                                                                           ggCTATTGTAGTCACTCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAg                                                >  1:1043523/1‑140 (MQ=255)                                                                           ggCTATTGTAGTCACTCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAg                                                >  2:685695/1‑140 (MQ=255)                                                                                 tGTAGTCACTCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATATTGCCTTTAATCgcgc                                                  <  1:391526/132‑1 (MQ=255)                                                                                 tGTAGTCACTCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATATTGCCTTTAATCgcgc                                                  >  2:391526/1‑132 (MQ=255)                                                                                         cTCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTAACGCCGCGt                                  <  1:594084/140‑1 (MQ=255)                                                                                          tCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTAACGCCGCGTa                                 >  1:980659/1‑140 (MQ=255)                                                                                          tCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTAACGCCGCGTa                                 >  1:23648/1‑140 (MQ=255)                                                                                                aTCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTAACGCCGCGTATCGtt                            >  2:526189/1‑140 (MQ=255)                                                                                                     ccGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTAACGCCGCGTATCGTTTTCAc                       >  2:802155/1‑140 (MQ=255)                                                                                                     ccGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTAACGCCGCGTATCGTTTTCAc                       >  1:892581/1‑140 (MQ=255)                                                                                                      cGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTAACGCCGCGTATCGTTTTCACg                      <  1:526189/140‑1 (MQ=255)                                                                                                       gCTGGCAGGCAAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTAACGCCGCGTATCGTTTTCACgg                     >  2:994817/1‑140 (MQ=255)                                                                                                                caAAAAAGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTAACGCCGCGTATCGTTTTCACGGCAACGGATg            <  1:958920/140‑1 (MQ=255)                                                                                                                     aaGCCATTACCATTGAAGAGCTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTAACGCCGCGTATCGTTTTCACGGCAACGGATGCTGAc       <  2:278849/140‑1 (MQ=255)                                                                                                                          aTTACCATTGAAGAGCTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTAACGCCGCGTATCGTTTTCACGGCAACGGATGCTGACGTCAt  <  2:23648/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                              GATTTCGCTATCGCCACAGAAGCGCTGCATCTGTATGAAGATTTAGTGATGTTACCGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGG‑ATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAACTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTAACGCCGCGTATCGTTTTCACGGCAACGGATGCTGACGTCAT  >  NC_000913/1334284‑1334543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑