Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,338,156	T→C	S776P (TCC→CCC)	acnA →	aconitate hydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,338,156	0	T	C	100.0%	205.4 / NA	58	S776P (TCC→CCC)	acnA	aconitate hydratase 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/25);  total (33/25)

TGGTGCCTGGCGTTGAAGGGGGGATGACGCGGCATTTACCTGACAGCGACGTAGTCTCTATTTATGATGCTGCGATGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCTCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTGTGGTGATTGCCGAATCGTTTGAACGAATTCACCGTTCGAATTTAATTGGCATGGGCATCCTGCCGCTGGAATTTCCGCAAGGCGTAAC  >  NC_000913/1338019‑1338292                                                                                                                                          |                                                                                                                                         tGGTGCCTGGCGTTGAAGGGGGGATGACGCGGCATTTACCTGACAGCGACGTAGTCTCTATTTATGATGCTGCGATGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGcccc                                                                                                                                        >  1:344880/1‑140 (MQ=255)   gTGCCTGGCGTTGAAGGGGGGATGACGCGGCATTTACCTGACAGCGACGTAGTCTCTATTTATGATGCTGCGATGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAg                                                                                                                                      >  2:312176/1‑140 (MQ=255)    tGCCTGGCGTTGAAGGGGGGATGACGCGGCATTTACCTGACAGCGACGTAGTCTCTATTTATGATGCTGCGATGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGt                                                                                                                                     >  2:802974/1‑140 (MQ=255)         ggCGTTGAAGGGGGGATGACGCGGCATTTACCTGACAGCGACGTAGTCTCTATTTATGATGCTGCGATGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGa                                                                                                                                <  2:128391/140‑1 (MQ=255)             ttGAAGGGGGGATGACGCGGCATTTACCTGACAGCGACGTAGTCTCTATTTATGATGCTGCGATGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTgg                                                                                                                            >  2:628874/1‑140 (MQ=255)               gAAGGGGGGATGACGCGGCATTTACCTGACAGCGACGTAGTCTCTATTTATGATGCTGCGATGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGggc                                                                                                                          <  2:518903/140‑1 (MQ=255)                             gcgGCATTTACCTGACAGCGACGTAGTCTCTATTTATGATGCTGCGATGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCcgc                                                                                                            >  2:274465/1‑140 (MQ=255)                             gcgGCATTTACCTGACAGCGACGTAGTCTCTATTTATGATGCTGCGATGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCcgc                                                                                                            <  1:801801/140‑1 (MQ=255)                               ggCATTTACCTGACAGCGACGTAGTCTCTATTTATGATGCTGCGATGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCaa                                                                                                                     >  2:30906/1‑129 (MQ=255)                               ggCATTTACCTGACAGCGACGTAGTCTCTATTTATGATGCTGCGATGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCaa                                                                                                                     <  1:30906/129‑1 (MQ=255)                                   tttACCTGACAGCGACGTAGTCTCTATTTATGATGCTGCGATGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGc                                                                                                      <  1:628874/140‑1 (MQ=255)                                    ttACCTGACAGCGACGTAGTCTCTATTTATGATGCTGCGATGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCt                                                                                                     <  1:407246/140‑1 (MQ=255)                                         tGACAGCGACGTAGTCTCTATTTATGATGCTGCGATGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTa                                                                                                <  1:571105/140‑1 (MQ=255)                                          gACAGCGACGTAGTCTCTATTTATGATGCTGCGATGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTAt                                                                                               <  1:366053/140‑1 (MQ=255)                                           aCAGCGACGTAGTCTCTATTTATGATGCTGCGATGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCt                                                                                                     <  1:416509/133‑1 (MQ=255)                                           aCAGCGACGTAGTCTCTATTTATGATGCTGCGATGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCt                                                                                                     >  2:416509/1‑133 (MQ=255)                                               cGACGTAGTCTCTATTTATGATGCTGCGATGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCgtg                                                                                          >  2:1023143/1‑140 (MQ=255)                                                  cGTAGTCTCTATTTATGATGCTGCGATGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTgg                                                                                                  >  2:378638/1‑129 (MQ=255)                                                  cGTAGTCTCTATTTATGATGCTGCGATGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTgg                                                                                                  <  1:378638/129‑1 (MQ=255)                                                   gTAGTCTCTATTTATGATGCTGCGATGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTgtggt                                                                                      <  1:841958/140‑1 (MQ=255)                                                            aTTTATGATGCTGCGATGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTgtggt                                                                                      >  1:688686/1‑131 (MQ=255)                                                            aTTTATGATGCTGCGATGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTgtggt                                                                                      <  2:688686/131‑1 (MQ=255)                                                             tttATGATGCTGCGATGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTGTGGTGATTGCCGaa                                                                            <  1:474568/140‑1 (MQ=255)                                                              ttATGATGCTGCGATGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTGTGGTGATTGCCGAAt                                                                           >  1:129095/1‑140 (MQ=255)                                                                 tgatgCTGCGATGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTGTGGTGATTGCCGAATCGt                                                                        >  2:883563/1‑140 (MQ=255)                                                                  gatgCTGCGATGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTGTGGTGATTGCCGAATCGtt                                                                       <  2:1102006/140‑1 (MQ=255)                                                                    tgctgcGATGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTGTGGTGATTGCCGAATCGTTTg                                                                     <  1:802974/140‑1 (MQ=255)                                                                     gctgcGATGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTGTGGTGATTGCCGAATCGTTTGa                                                                    >  2:287217/1‑140 (MQ=255)                                                                     gctgcGATGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTGTGGTGATTGCCGAATCGTTTGa                                                                    >  1:1139894/1‑140 (MQ=255)                                                                       tgcGATGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTGTGGTGATTGCCGAATCGTTTGAAc                                                                  >  1:588868/1‑140 (MQ=255)                                                                       tgcGATGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTGTGGTGATTGCCGAATCGTTTGAAc                                                                  >  1:1038215/1‑140 (MQ=255)                                                                       tgcGATGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTGTGGTGATTGCCGAATCGTTTGAAc                                                                  >  2:628000/1‑140 (MQ=255)                                                                         cGATGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTGTGGTGATTGCCGAATCGTTTGAACGa                                                                <  1:924548/140‑1 (MQ=255)                                                                           aTGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTGTGGTGATTGCCGAATCGTTTGAACGAAt                                                              >  2:1032217/1‑140 (MQ=255)                                                                              cgcTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTGTGGTGATTGCCGAATCGTTTGAACGAATTCa                                                           <  2:129095/140‑1 (MQ=255)                                                                                        aGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTGTGGTGATTGCCGAATCGTTTGAACGAATTCACCGtt                                                      >  2:454987/1‑135 (MQ=255)                                                                                        aGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTGTGGTGATTGCCGAATCGTTTGAACGAATTCACCGtt                                                      <  1:454987/135‑1 (MQ=255)                                                                                        aGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTGTGGTGATTGCCGAATCGTTTGAACGAATTCACCGTTCGAAt                                                 >  1:1032375/1‑140 (MQ=255)                                                                                        aGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTAGTGTGGTGATTGCCGAATCGTTTGAACGAATTCACCGTTCGAAt                                                 >  2:416586/1‑140 (MQ=255)                                                                                          gAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTGTGGTGATTGCCGAATCGTTTGAACGAATTCACCGTTCGAAttt                                               >  2:1145827/1‑140 (MQ=255)                                                                                           aGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTGTGGTGATTGCCGAATCGTTTGAACGAATTCACCGTTCGAATTTa                                              >  2:479895/1‑140 (MQ=255)                                                                                           aGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTGTGGTGATTGCCGAATCGTTTGAACGAATTCACCGTTCGAATTTa                                              >  1:593653/1‑140 (MQ=255)                                                                                           aGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTGTGGTGATTGCCGAATCGTTTGAACGAATTCACCGTTCGAATTTa                                              >  1:1005745/1‑140 (MQ=255)                                                                                             cAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTGTGGTGATTGCCGAATCGTTTGAACGAATTCACCGTTCGAATTTAAt                                            >  2:933269/1‑140 (MQ=255)                                                                                             cAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTGTGGTGATTGCCGAATCGTTTGAACGAATTCACCGTTCGAATTTAAt                                            >  2:334723/1‑140 (MQ=255)                                                                                               aaCGCCGCTGGCGGTGATTGCCGGGAAAGCGTATGGGTCAGGCCCCAGTCGGGACTTGGCGGCAACAGGTCCGCGTCTGCTTGGGATTCGCGTGGCTATTGCCGAATAGTTTGCAGCAATTCTATGCTCGCATTGAATTg                                          >  2:449404/1‑140 (MQ=255)                                                                                                aCGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTGTGGTGATTGCCGAATCGTTTGAACGAATTCACCGTTCGAATTTAATTgg                                         <  1:479895/140‑1 (MQ=255)                                                                                                 cgccgcTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTGTGGTGATTGCCGAATCGTTTGAACGAATTCACCGTTCGAATTTAATTGGc                                        <  1:312176/140‑1 (MQ=255)                                                                                                  gccgcTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTGTGGTGATTGCCGAATCGTTTGAACGAATTCACCGTTCGAATTTAATTGGCa                                       <  1:628000/140‑1 (MQ=255)                                                                                                   ccgcTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTCCTTGGTATTCGTGTGGTGATTGCCGAATCGTTTGAACGAATTCACCGTTCGAATTTAATTGGCAt                                      <  1:1023143/140‑1 (MQ=255)                                                                                                      cTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTGTGGTGATTGCCGAATCGTTTGAACGAATTCACCGTTCGAATTTAATTGGCATggg                                   >  1:677718/1‑140 (MQ=255)                                                                                                                   ccGGGAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTGTGGTGATTGCCGAATCGTTTGAACGAATTCACCGTTCGAATTTAATTGGCATGGGCATCCTGCCGCTg                      >  2:424004/1‑140 (MQ=255)                                                                                                                     gggAAAGAGTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTGTGGTGATTGCCGAATCGTTTGAACGAATTCACCGTTCGAATTTAATTGGCATGGGCATCCTGCCGCTGGa                    >  2:629693/1‑140 (MQ=255)                                                                                                                             gTATGGATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTGTGGTGATTGCCGAATCGTTTGAACGAATTCACCGTTCGAATTTAATTGGCATGGGCATCCTGCCGCTGGAATTTCCGc            <  2:1032375/140‑1 (MQ=255)                                                                                                                                 ggATCAGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTGTGGTGATTGCCGAATCGTTTGAACGAATTCACCGTTCGAATTTAATTGGCATGGGCATCCTGCCGCTGGAATTTCCGCAAgg        >  2:59471/1‑140 (MQ=255)                                                                                                                                      aGGCCCCAGTCGTGCCTGGGCGGCAAAAGGTCCGCGTCTGCTTGGAATTCGTGTGG‑GATTGCCGGATCGTTTGAACGAATTCCCCGGTCGAATTTAATTGGCATGGGCATCCTGCCGCTGGAATTTCCGCAAGGCGTAAc  >  2:315739/1‑140 (MQ=255)                                                                                                                                      aGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTGTGGTGATTGCCGAATCGTTTGAACGAATTCACCGTTCGAATTTAATTGGCATGGGCATCCTGCCGCTGGAATTTCCGCAAGGCGTaa   <  2:1005745/140‑1 (MQ=255)                                                                                                                                      aGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTGTGGTGATTGCCGAATCGTTTGAACGAATTCACCGTTCGAATTTAATTGGCATGGGCATCCTGCCGCTGGAATTTCCGCAAGGCGTaa   >  2:595727/1‑140 (MQ=255)                                                                                                                                      aGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTGTGGTGATTGCCGAATCGTTTGAACGAATTCACCGTTCGAATTTAATTGGCATGGGCATCCTGCCGCTGGAATTTCCGCAAGGCGTaa   >  1:572168/1‑140 (MQ=255)                                                                                                                                      aGGCCCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTGTGGTGATTGCCGAATCGTTTGAACGAATTCACCGTTCGAATTTAATTGGCATGGGCATCCTGCCGCTGGAATTTCCGCAAGGCGTaa   <  1:287217/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         TGGTGCCTGGCGTTGAAGGGGGGATGACGCGGCATTTACCTGACAGCGACGTAGTCTCTATTTATGATGCTGCGATGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCTCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTGTGGTGATTGCCGAATCGTTTGAACGAATTCACCGTTCGAATTTAATTGGCATGGGCATCCTGCCGCTGGAATTTCCGCAAGGCGTAAC  >  NC_000913/1338019‑1338292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑