Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,385,351 A→G Y412C (TAT→TGT)  ycjX → DUF463 domain‑containing protein YcjX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,385,3510AG100.0% 153.1 / NA 44Y412C (TAT→TGT) ycjXDUF463 domain‑containing protein YcjX
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/22);  total (22/22)

GCCTGGGGCTGGCGTCAGTTCAGGCGACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACCGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCATATTCGTCTTGATGCTGCGCTGGAA  >  NC_000913/1385237‑1385487
                                                                                                                  |                                                                                                                                        
gCCTGGGGCTGGCGTCAGTTCAGGCGACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACCGCTCACTGTTTGTCCTGGCGAAGTTCCCGCACGTTTg                                                                                                                 >  1:859853/1‑140 (MQ=255)
gCCTGGGGCTGGCGTCAGTTCAGGCGACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACCGCTCACTGTTTGTCCTGGCGAAGTTCCCGCACGTTTg                                                                                                                 >  2:687337/1‑140 (MQ=255)
  cTGGGGCTGGCGTCAGTTCAGGCGACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACCGCTCACTGTTTGTCCTGGCGAAGTTCCCGCACGTTTGcc                                                                                                               >  2:631118/1‑140 (MQ=255)
         tGGCGTCAGTTCAGGCGACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACCGCTCACTGTTTGTCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAg                                                                                                        <  1:631118/140‑1 (MQ=255)
          ggCGTCAGTTCAGGCGACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACCGCTCACTGTTTGTCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAgg                                                                                                       <  2:432317/140‑1 (MQ=255)
           gCGTCAGTTCAGGCGACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACCGCTCACTGTTTGTCCTGGCGAAGTTCCCGCACGTTTGCCTg                                                                                                             <  1:1023937/133‑1 (MQ=255)
           gCGTCAGTTCAGGCGACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACCGCTCACTGTTTGTCCTGGCGAAGTTCCCGCACGTTTGCCTg                                                                                                             >  2:1023937/1‑133 (MQ=255)
           gCGTCAGTTCAGGCGACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACCGCTCACTGTTTGTCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGc                                                                                                      >  2:841495/1‑140 (MQ=255)
           gCGTCAGTTCAGGCGACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACCGCTCACTGTTTGTCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGc                                                                                                      >  2:1156658/1‑140 (MQ=255)
             gTCAGTTCAGGCGACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACCGCTCACTGTTTGTCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGt                                                                                                    <  1:841495/140‑1 (MQ=255)
              tCAGTTCAGGCGACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACCGCTCACTGTTTGTCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGtt                                                                                                   >  1:312975/1‑140 (MQ=255)
              tCAGTTCAGGCGACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACCGCTCACTGTTTGTCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGtt                                                                                                   <  1:876164/140‑1 (MQ=255)
                   tCAGGCGACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACCGCTCACTGTTTGTCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTggg                                                                                              <  2:689184/140‑1 (MQ=255)
                     aGGCGACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACCGCTCACTGTTTGTCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTc                                                                                                  >  1:321957/1‑134 (MQ=255)
                     aGGCGACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACCGCTCACTGTTTGTCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTc                                                                                                  <  2:321957/134‑1 (MQ=255)
                         gACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACCGCTCACTGTTTGTCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGc                                                                                        <  1:1127611/140‑1 (MQ=255)
                                      attattGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACCGCTCACTGTTTGTCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGtt                                                                           >  1:1119125/1‑140 (MQ=255)
                                               gTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACCGCTCACTGTTTGTCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCAtt                                                                  >  1:971151/1‑140 (MQ=255)
                                               gTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACCGCTCACTGTTTGTCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCAtt                                                                  >  2:402488/1‑140 (MQ=255)
                                                   aCGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACCGCTCACTGTTTGTCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGt                                                              >  2:79265/1‑140 (MQ=255)
                                                    tggTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACCGCTCACTGTTTGTCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTc                                                             >  1:288951/2‑140 (MQ=255)
                                                    cGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACCGCTCACTGTTTGTCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTc                                                             <  1:788355/140‑1 (MQ=255)
                                                      gTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACCGCTCACTGTTTGTCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCg                                                           <  1:1160869/140‑1 (MQ=255)
                                                      gTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACCGCTCACTGTTTGTCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCg                                                           <  2:288951/140‑1 (MQ=255)
                                                        gagaAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACCGCTCACTGTTTGTCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc                                                          <  2:569434/139‑1 (MQ=255)
                                                        gagaAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACCGCTCACTGTTTGTCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc                                                          >  1:569434/1‑139 (MQ=255)
                                                             aaTCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACCGCTCACTGTTTGTCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTga                                                    >  1:474553/1‑140 (MQ=255)
                                                               tCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACCGCTCACTGTTTGTCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTgatg                                                  >  1:924256/1‑140 (MQ=255)
                                                                cccGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACCGCTCACTGTTTGTCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTgatgg                                                 <  2:672799/140‑1 (MQ=255)
                                                                     cgcTGCGTGGTAATCGACTTAGCGATGGCGCACCGCTCACTGTTTGTCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTgatgga                                                <  2:1163389/136‑1 (MQ=255)
                                                                     cgcTGCGTGGTAATCGACTTAGCGATGGCGCACCGCTCACTGTTTGTCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTgatgga                                                >  1:1163389/1‑136 (MQ=255)
                                                                       cTGCGTGGTAATCGACTTAGCGATGGCGCACCGCTCACTGTTTGTCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGa                                          <  2:800805/140‑1 (MQ=255)
                                                                         gCGTGGTAATCGACTTAGCGATGGCGCACCGCTCACTGTTTGTCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACa                                        <  1:1156658/140‑1 (MQ=255)
                                                                               tAATCGACTTAGCGATGGCGCACCGCTCACTGTTTGTCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCAc                                  <  2:924256/140‑1 (MQ=255)
                                                                                       ttAGCGATGGCGCACCGCTCACTGTTTGTCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCat                          >  2:501691/1‑140 (MQ=255)
                                                                                        tAGCGATGGCGCACCGCTCACTGTTTGTCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCg                             <  2:97833/136‑1 (MQ=255)
                                                                                        tAGCGATGGCGCACCGCTCACTGTTTGTCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCg                             >  1:97833/1‑136 (MQ=255)
                                                                                         aGCGATGGCGCACCGCTCACTGTTTGTCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCatat                        >  1:660310/1‑140 (MQ=255)
                                                                                                    aCCGCTCACTGTTTGTCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCATATTCGTCTTGAtg             >  2:550483/1‑140 (MQ=255)
                                                                                                        cTCACTGTTTGTCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCATATTCGTCTTGAtgctgc         >  2:963694/1‑140 (MQ=255)
                                                                                                              gTTTGTCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCATATTCGTCTTGATGCTGCGCTGGa   <  2:971151/140‑1 (MQ=255)
                                                                                                               tttGTCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCATATTCGTCTTGATGCTGCGCTGGaa  <  1:963262/140‑1 (MQ=255)
                                                                                                               tttGTCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCATATTCGTCTTGATGCTGCGCTGGaa  <  1:79265/140‑1 (MQ=255)
                                                                                                               tttGTCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCATATTCGTCTTGATGCTGCGCTGGaa  <  2:660310/140‑1 (MQ=255)
                                                                                                               tttGTCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCATATTCGTCTTGATGCTGCGCTGGaa  <  2:859853/140‑1 (MQ=255)
                                                                                                                  |                                                                                                                                        
GCCTGGGGCTGGCGTCAGTTCAGGCGACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACCGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCATATTCGTCTTGATGCTGCGCTGGAA  >  NC_000913/1385237‑1385487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑