Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,505,587	G→A	A165V (GCG→GTG)	ydcO ←	putative transport protein YdcO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,505,587	0	G	A	100.0%	163.4 / NA	47	A165V (GCG→GTG)	ydcO	putative transport protein YdcO
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/21);  total (26/21)

CAAACGAAAAATCAGGGGTAATATAAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGG  >  NC_000913/1505456‑1505720                                                                                                                                    |                                                                                                                                      cAAACGAAAAATCAGGGGTAATATAAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCACAACGGcc                                                                                                                               <  1:598085/140‑1 (MQ=255)   aaCGAAAAATCAGGGGTAATATAAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCACAACGGCCtt                                                                                                                             <  2:788531/140‑1 (MQ=255)    aCGAAAAATCAGGGGTAATATAAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCACAACGGCCTTg                                                                                                                            >  2:997455/1‑140 (MQ=255)          aaTCAGGGGTAATATAAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCACAACGGCCTTGGTTgcc                                                                                                                      <  2:501161/140‑1 (MQ=255)              aGGGGTAATATAAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCACAACGGCCTTGGTTgccagcc                                                                                                                  <  1:997455/140‑1 (MQ=255)              aGGGGTAATATAAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCACAACGGCCTTGGTTgccagcc                                                                                                                  >  2:503344/1‑140 (MQ=255)                     atatAAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCACAACGGCCTTGGTTGCCAGCCATACCAg                                                                                                           >  1:8026/1‑140 (MQ=255)                          aGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCACAACGGCCTTGGTTGCCAGCCATACCAGCAACa                                                                                                      <  2:501458/140‑1 (MQ=255)                          aGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCACAACGGCCTTGGTTGCCAGCCATACCAGCAACa                                                                                                      >  1:676010/1‑140 (MQ=255)                          aGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCACAACGGCCTTGGTTGCCAGCCATACCAGCAACa                                                                                                      <  1:503344/140‑1 (MQ=255)                           gTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCACAACGGCCTTGGTTGCCAGCCATACCAGCAACAt                                                                                                     >  2:738158/1‑140 (MQ=255)                               ggAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCACAACGGCCTTGGTTGCCAGCCATACCAGCAACATACtt                                                                                                 >  2:552190/1‑140 (MQ=255)                                gagaACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCACAACGGCCTTGGTTGCCAGCCATACCAGCAACa                                                                                                      >  2:775293/1‑134 (MQ=255)                                gagaACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCACAACGGCCTTGGTTGCCAGCCATACCAGCAACa                                                                                                      <  1:775293/134‑1 (MQ=255)                                gagaACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCACAACGGCCTTGGTTGCCAGCCATACCAGCAACa                                                                                                      <  2:931644/134‑1 (MQ=255)                                gagaACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCACAACGGCCTTGGTTGCCAGCCATACCAGCAACa                                                                                                      >  1:931644/1‑134 (MQ=255)                                  gaACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCACAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCca                                                                                              >  2:358044/1‑140 (MQ=255)                                  gaACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCACAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCca                                                                                              <  2:904159/140‑1 (MQ=255)                                   aaCGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCACAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCcac                                                                                             <  1:1147720/140‑1 (MQ=255)                                   aaCGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCACAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCcac                                                                                             >  2:1147720/1‑140 (MQ=255)                                     cGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCACAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCcacac                                                                                           <  1:552190/140‑1 (MQ=255)                                       ggTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCACAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACaa                                                                                         <  2:8026/140‑1 (MQ=255)                                        gTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCACAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAAc                                                                                        >  1:89266/1‑140 (MQ=255)                                                 caacaTCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCACAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGa                                                                               <  1:124056/140‑1 (MQ=255)                                                                 aaCGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCACAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTc                                                                          >  2:599713/1‑129 (MQ=255)                                                                 aaCGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCACAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTc                                                                          <  1:599713/129‑1 (MQ=255)                                                                   cGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCACAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTGGCAAAc                                                             <  2:89266/140‑1 (MQ=255)                                                                         cTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCACAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTGGCAAACGCCTGt                                                       <  1:432801/140‑1 (MQ=255)                                                                               cgATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCACAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCa                                                 >  2:1109512/1‑140 (MQ=255)                                                                               cgATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCACAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCa                                                 <  2:1150324/140‑1 (MQ=255)                                                                                            gatcCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCACAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATaaaa                                    >  2:990056/1‑140 (MQ=255)                                                                                              tcCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCACAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATc                                  >  1:5350/1‑140 (MQ=255)                                                                                                        catcGCGGCAATTACCGCATAGCGCGGCACAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAAT‑AAATCCCGGCCAGCAt                       >  1:54580/1‑140 (MQ=255)                                                                                                         atcGCGGCAATTACCGCATAGCGCGGCACAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCAt                       >  2:2481/1‑140 (MQ=255)                                                                                                         atcGCGGCAATTACCGCATAGCGCGGCACAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCAt                       >  2:216685/1‑140 (MQ=255)                                                                                                          tcGCGGCAATTACCGCATAGCGCGGCACAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCAtt                      >  1:24246/1‑140 (MQ=255)                                                                                                          tcGCGGCAATTACCGCATAGCGCGGCACAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCAtt                      >  1:1132634/1‑140 (MQ=255)                                                                                                           cgcgGCAATTACCGCATAGCGCGGCACAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTg                     >  2:368963/1‑140 (MQ=255)                                                                                                                      ccGCATAGCGCGGCACAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGc          <  2:24246/140‑1 (MQ=255)                                                                                                                          aTAGCGCGGCACAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGt      <  1:358044/140‑1 (MQ=255)                                                                                                                           tAGCGCGGCACAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTg     <  1:990056/140‑1 (MQ=255)                                                                                                                              cgcgGCACAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGt      >  2:945685/1‑136 (MQ=255)                                                                                                                              cgcgGCACAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGt      <  1:945685/136‑1 (MQ=255)                                                                                                                              cgcgGCACAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCgg  >  1:371173/1‑140 (MQ=255)                                                                                                                              cgcgGCACAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCgg  >  1:320835/1‑140 (MQ=255)                                                                                                                              cgcgGCACAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCgg  >  1:408566/1‑140 (MQ=255)                                                                                                                              cgcgGCACAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCgg  >  2:212218/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                      CAAACGAAAAATCAGGGGTAATATAAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGG  >  NC_000913/1505456‑1505720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑