| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,541,351 | C→T | E414K (GAA→AAA) | narZ ← | nitrate reductase Z subunit alpha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,541,351 | 0 | C | T | 95.2% | 133.2 / ‑1.8 | 42 | E414K (GAA→AAA) | narZ | nitrate reductase Z subunit alpha |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (22/18); total (23/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01 | |||||||||||
GGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTCGGTTTCCGTACCGGCGGCAATGGATTCCAGAT‑TCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACTGCGGATTGTTGCT > NC_000913/1541231‑1541486 | ggCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCa < 2:109496/140‑1 (MQ=255) cACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATgg > 2:454274/1‑140 (MQ=255) cACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATgg > 1:971986/1‑140 (MQ=255) aCCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGga < 2:758487/140‑1 (MQ=255) aGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGgattc > 2:13907/1‑140 (MQ=255) tgtgTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGAT‑TCCAt < 1:828206/140‑1 (MQ=255) tgtTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGAT‑TCCAttt > 2:741139/1‑140 (MQ=255) tGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGAT‑TCCATTTGCCTTTTtct > 1:143707/1‑140 (MQ=255) tGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGAT‑TCCATTTGCCTTTTtct > 1:174465/1‑140 (MQ=255) tGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGAT‑TCCATTTGCCTTTTtct > 2:192405/1‑140 (MQ=255) aaTGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGAT‑TCCATTTGCCTTTTTCTCCCCAg < 2:257429/140‑1 (MQ=255) tGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGAT‑TCCATTTGCCTTTTTCTCCCCAGCg < 1:572103/140‑1 (MQ=255) ccAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGAT‑TCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGtt > 2:64329/1‑140 (MQ=255) aGGGGAAGGCCACGCCTGCACCAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGCTTCCGTACCGGCGGCAATATATTCCACTTATCCATTT‑CCTTTTTCTCCCCCGAGGAAACCAATCGAACCGTTCGGCACt > 2:170923/1‑140 (MQ=255) aGGGGAAGGCCACG‑CTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGAT‑TCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGt < 1:1019162/131‑1 (MQ=255) aGGGGAAGGCCACG‑CTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGAT‑TCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGt > 2:1019162/1‑131 (MQ=255) gggAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGAT‑TCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTAc > 1:358523/1‑140 (MQ=255) gAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGAT‑TCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGc > 2:291323/1‑133 (MQ=255) gAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGAT‑TCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGc < 1:291323/133‑1 (MQ=255) ggCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGAT‑TCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAAtt > 2:674703/1‑140 (MQ=255) ggCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGAT‑TCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAAtt > 1:562139/1‑140 (MQ=255) gCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGAT‑TCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACt < 2:437229/132‑1 (MQ=255) gCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGAT‑TCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACt > 1:437229/1‑132 (MQ=255) ccACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGAT‑TCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCa > 1:883317/1‑140 (MQ=255) ccACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTCGGTTTCCGTACCGGCGGCAATGGATTCCAGAT‑TCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCa > 1:411864/1‑140 (MQ=255) cGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGAT‑TCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCt < 2:143707/140‑1 (MQ=255) cGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGAT‑TCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCt < 2:541313/140‑1 (MQ=255) cGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGAT‑TCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCt < 2:1009267/140‑1 (MQ=255) cGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGAT‑TCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCt < 1:674703/140‑1 (MQ=255) gCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGAT‑TCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTg < 2:174465/140‑1 (MQ=255) gCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGAT‑TCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTg < 1:332033/140‑1 (MQ=255) gCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGAT‑TCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTg < 1:64329/140‑1 (MQ=255) gCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTCGGTTTCCGTACCGGCGGCAATGGATTCCAGAT‑TCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTg < 2:411864/140‑1 (MQ=255) cAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGAT‑TCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCCCTACCAATTCACCTGCGGTATTa < 2:163949/140‑1 (MQ=255) gCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGAT‑TCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAAc > 2:323809/1‑140 (MQ=255) cGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGAT‑TCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACt > 1:71556/1‑140 (MQ=255) tCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGAT‑TCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGc < 1:131028/140‑1 (MQ=255) tCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGAT‑TCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGc > 1:307330/1‑140 (MQ=255) aTGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGAT‑TCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTa < 1:848421/140‑1 (MQ=255) aTGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGAT‑TCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTa < 2:971986/140‑1 (MQ=255) gAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGAT‑TCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTcc > 1:465810/1‑140 (MQ=255) gCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGAT‑TCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCAc > 2:417082/1‑140 (MQ=255) cAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGAT‑TCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACt > 1:397732/1‑140 (MQ=255) aaTTTGGTTTCCGTACCGGCGGCAATGGATTCCAGAT‑TCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACTGCGGATTGTTGCt > 2:784897/1‑140 (MQ=255) | GGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTCGGTTTCCGTACCGGCGGCAATGGATTCCAGAT‑TCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACTGCGGATTGTTGCT > NC_000913/1541231‑1541486 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |