Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	143,016	T→C	L80L (TTG→CTG)	yadG →	putative ABC transporter ATP‑binding protein YadG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	143,016	0	T	C	100.0%	246.8 / NA	69	L80L (TTG→CTG)	yadG	putative ABC transporter ATP‑binding protein YadG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/28);  total (41/28)

TGCGCTTCTCGGGCCGAACGGGGCCGGGAAATCGACCACTATCGGTATTATCAGCTCTCTGGTAAATAAAACCTCCGGGCGGGTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGTTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGAAAAGTATCTTA  >  NC_000913/142880‑143142                                                                                                                                         |                                                                                                                               tGCGCTTCTCGGGCCGAACGGGGCCGGGAAATCGACCACTATCGGTATTATCAGCTCTCTGGTAAATAAAACCTCCGGGCGGGTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTgg                                                                                                                             >  1:852143/1‑140 (MQ=255) tGCGCTTCTCGGGCCGAACGGGGCCGGGAAATCGACCACTATCGGTATTATCAGCTCTCTGGTAAATAAAACCTCCGGGCGGGTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTgg                                                                                                                             >  2:411835/1‑140 (MQ=255)    gcTTCTCGGGCCGAACGGGGCCGGGAAATCGACCACTATCGGTATTATCAGCTCTCTGGTAAATAAAACCTCCGGGCGGGTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGAc                                                                                                                          <  1:59589/140‑1 (MQ=255)     cTTCTCGGGCCGAACGGGGCCGGGAAATCGACCACTATCGGTATTATCAGCTCTCTGGTAAATAAAACCTCCGGGCGGGTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACt                                                                                                                         <  2:751520/140‑1 (MQ=255)        ctcGGGCCGAACGGGGCCGGGAAATCGACCACTATCGGTATTATCAGCTCTCTGGTAAATAAAACCTCCGGGCGGGTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGt                                                                                                                      >  1:784485/1‑140 (MQ=255)            ggCCGAACGGGGCCGGGAAATCGACCACTATCGGTATTATCAGCTCTCTGGTAAATAAAACCTCCGGGCGGGTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCg                                                                                                                  <  1:46696/140‑1 (MQ=255)                  aCGGGGCCGGGAAATCGACCACTATCGGTATTATCAGCTCTCTGGTAAATAAAACCTCCGGGCGGGTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGAc                                                                                                                          >  2:175143/1‑126 (MQ=255)                  aCGGGGCCGGGAAATCGACCACTATCGGTATTATCAGCTCTCTGGTAAATAAAACCTCCGGGCGGGTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGAc                                                                                                                          <  1:175143/126‑1 (MQ=255)                  aCGGGGCCGGGAAATCGACCACTATCGGTATTATCAGCTCTCTGGTAAATAAAACCTCCGGGCGGGTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGaa                                                                                                            >  2:1092769/1‑140 (MQ=255)                       gCCGGGAAATCGACCACTATCGGTATTATCAGCTCTCTGGTAAATAAAACCTCCGGGCGGGTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTaa                                                                                                       >  1:1035057/1‑140 (MQ=255)                       gCCGGGAAATCGACCACTATCGGTATTATCAGCTCTCTGGTAAATAAAACCTCCGGGCGGGTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTaa                                                                                                       >  2:355691/1‑140 (MQ=255)                        ccGGGAAATCGACCACTATCGGTATTATCAGCTCTCTGGTAAATAAAACCTCCGGGCGGGTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAAc                                                                                                      >  1:999391/1‑140 (MQ=255)                           ggAAATCGACCACTATCGGTATTATCAGCTCTCTGGTAAATAAAACCTCCGGGCGGGTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTc                                                                                                   >  2:46158/1‑140 (MQ=255)                                      aCTATCGGTATTATCAGCTCTCTGGTAAATAAAACCTCCGGGCGGGTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGa                                                                                        <  1:1092769/140‑1 (MQ=255)                                        tATCGGTATTATCAGCTCTCTGGTAAATAAAACCTCCGGGCGGGTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGaaa                                                                                      <  2:127052/140‑1 (MQ=255)                                        tATCGGTATTATCAGCTCTCTGGTAAATAAAACCTCCGGGCGGGTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGaaa                                                                                      <  1:411835/140‑1 (MQ=255)                                           cGGTATTATCAGCTCTCTGGTAAATAAAACCTCCGGGCGGGTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCg                                                                                   <  2:784485/140‑1 (MQ=255)                                            ggTATTATCAGCTCTCTGGTAAATAAAACCTCCGGGCGGGTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGt                                                                                  <  2:1035057/140‑1 (MQ=255)                                                 tatCAGCTCTCTGGTAAATAAAACCTCCGGGCGGGTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTgcagc                                                                             >  2:368806/1‑140 (MQ=255)                                                  atCAGCTCTCTGGTAAATAAAACCTCCGGGCGGGTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTgcagca                                                                            >  1:624962/1‑140 (MQ=255)                                                  atCAGCTCTCTGGTAAATAAAACCTCCGGGCGGGTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTgcagca                                                                            >  2:666627/1‑140 (MQ=255)                                                  atCAGCTCTCTGGTAAATAAAACCTCCGGGCGGGTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTgcagca                                                                            >  2:1025443/1‑140 (MQ=255)                                                      gCTCTCTGGTAAATAAAACCTCCGGGCGGGTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAAtt                                                                        >  2:349744/1‑140 (MQ=255)                                                      gCTCTCTGGTAAATAAAACCTCCGGGCGGGTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAAtt                                                                        >  2:1020709/1‑140 (MQ=255)                                                         ctctGGTAAATAAAACCTCCGGGCGGGTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATtgtg                                                                     <  2:727480/140‑1 (MQ=255)                                                          tctGGTAAATAAAACCTCCGGGCGGGTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTgtgg                                                                    >  2:318928/1‑140 (MQ=255)                                                          tctGGTAAATAAAACCTCCGGGCGGGTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTgtgg                                                                    >  1:137543/1‑140 (MQ=255)                                                            tGGTAAATAAAACCTCCGGGCGGGTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTgtggtg                                                                  >  1:339198/1‑140 (MQ=255)                                                            tGGTAAATAAAACCTCCGGGCGGGTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTgtggtg                                                                  >  1:635463/1‑140 (MQ=255)                                                                aaataaaACCTCCGGGCGGGTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATc                                                              <  2:181193/140‑1 (MQ=255)                                                                   taaaACCTCCGGGCGGGTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATcagg                                                           <  2:999391/140‑1 (MQ=255)                                                                   taaaACCTCCGGGCGGGTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATcagg                                                           <  1:1025443/140‑1 (MQ=255)                                                                        ccTCCGGGCGGGTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATcagg                                                           >  1:911344/1‑135 (MQ=255)                                                                        ccTCCGGGCGGGTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATcagg                                                           <  2:911344/135‑1 (MQ=255)                                                                           ccGGGCGGGTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATcaggca                                                         <  1:538744/134‑1 (MQ=255)                                                                           ccGGGCGGGTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATcaggca                                                         >  2:538744/1‑134 (MQ=255)                                                                           ccGGGCGGGTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGtac                                                   >  1:208223/1‑140 (MQ=255)                                                                            cgggcgggTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGtact                                                  >  1:952204/1‑140 (MQ=255)                                                                            cgggcgggTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGtact                                                  <  1:368806/140‑1 (MQ=255)                                                                             gggcgggTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGtacta                                                 >  2:443883/1‑140 (MQ=255)                                                                                cgggTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACgg                                              >  2:258172/1‑140 (MQ=255)                                                                                  ggTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCg                                            >  1:976961/1‑140 (MQ=255)                                                                                  ggTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCg                                            >  2:1115250/1‑140 (MQ=255)                                                                                   gTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGt                                           <  1:1115250/140‑1 (MQ=255)                                                                                   gTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGt                                           >  1:1115272/1‑140 (MQ=255)                                                                                    tCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTg                                          <  2:429870/140‑1 (MQ=255)                                                                                         gTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAgcg                                     >  1:99163/1‑140 (MQ=255)                                                                                         gTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAgcg                                     >  1:448979/1‑140 (MQ=255)                                                                                           aTTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCa                                   <  1:46158/140‑1 (MQ=255)                                                                                             ttGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCaaa                                 >  1:1115196/1‑140 (MQ=255)                                                                                             ttGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCaaa                                 <  1:1020709/140‑1 (MQ=255)                                                                                                 ttACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAaagaag                             <  1:318928/140‑1 (MQ=255)                                                                                                 ttACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAaagaag                             <  2:952204/140‑1 (MQ=255)                                                                                                          cGAGAAGGATGTCGTGAACGCTAAACGTCAGCTTGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATcc                    >  2:528088/1‑140 (MQ=255)                                                                                                            agaAGGATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGc                  <  2:852143/140‑1 (MQ=255)                                                                                                                ggATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCg              >  2:60666/1‑140 (MQ=255)                                                                                                                 gATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGa             >  1:159828/1‑140 (MQ=255)                                                                                                                 gATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGa             >  2:270606/1‑140 (MQ=255)                                                                                                                 gATGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGa             >  1:586838/1‑140 (MQ=255)                                                                                                                  aTGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGaa            >  2:181726/1‑140 (MQ=255)                                                                                                                   tGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGaaa           >  2:321471/1‑140 (MQ=255)                                                                                                                   tGTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGaaa           >  2:308004/1‑140 (MQ=255)                                                                                                                    gTCGTGAACGCTAAACGTCAGCTTGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGaaaa          <  1:528088/140‑1 (MQ=255)                                                                                                                    gTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGaaaa          <  2:339198/140‑1 (MQ=255)                                                                                                                    gTCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGaaaa          <  2:1115272/140‑1 (MQ=255)                                                                                                                     tCGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGCGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGAAAAg         >  1:913766/1‑140 (MQ=255)                                                                                                                      cGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGAAAAGt        <  2:448979/140‑1 (MQ=255)                                                                                                                      cGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGAAAAGt        <  2:99163/140‑1 (MQ=255)                                                                                                                      cGTGAACGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGAAAAGt        <  1:355691/140‑1 (MQ=255)                                                                                                                            cGCTAAACGTCAGCTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGAAAAGTATCTTa  >  1:1078272/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                               TGCGCTTCTCGGGCCGAACGGGGCCGGGAAATCGACCACTATCGGTATTATCAGCTCTCTGGTAAATAAAACCTCCGGGCGGGTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGTTGGGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGAAAAGTATCTTA  >  NC_000913/142880‑143142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑