Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,686,087	T→C	T168A (ACA→GCA)	fumC ←	fumarase C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,686,087	0	T	C	100.0%	148.4 / NA	43	T168A (ACA→GCA)	fumC	fumarase C
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/18);  total (25/18)

TGAGATTATGCTCGAGCATCGCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGGTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCTTTTGTTCACGTCGTCGTTAGGGTGAACTTTACGTTCCATCCC  >  NC_000913/1685952‑1686222                                                                                                                                        |                                                                                                                                        tGAGATTATGCTCGAGCATCGCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGCCa                                                                                                                                       >  2:996851/1‑138 (MQ=255)        aTGCTCGAGCATCGCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGCCAGGGTTTTaa                                                                                                                              <  1:1012672/140‑1 (MQ=255)         tGCTCGAGCATCGCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTATGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGCCAGGGTTTTAAg                                                                                                                             >  2:324568/1‑140 (MQ=255)           cTCGAGCATCGCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGCCAGGGTTTTAAGCt                                                                                                                           <  2:1000767/140‑1 (MQ=255)              gAGCATCGCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGCCAGGGTTTTAAGCTGAg                                                                                                                        >  1:917863/1‑140 (MQ=255)              gAGCATCGCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGCCAGGGTTTTAAGCTGAg                                                                                                                        >  1:707746/1‑140 (MQ=255)               aGCATCGCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGCCa                                                                                                                                       >  1:640432/1‑124 (MQ=255)                   tCGCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGCCAGGGTTTTAAGCTGAGGAATg                                                                                                                   >  1:973456/1‑140 (MQ=255)                    cGCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGCCAGGGTTTTAAGCTGAGGAATGa                                                                                                                  <  1:1008820/140‑1 (MQ=255)                    cGCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGCCAGGGTTTTAAGCTGAGGAATGa                                                                                                                  >  2:523037/1‑140 (MQ=255)                          ccAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGCCAGGGTTTTAAGCTGAGGAATGAGttgct                                                                                                            <  1:523037/140‑1 (MQ=255)                             gCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGCCAGGGTTTTAAGCTGAGGAATGAGttgcttgc                                                                                                         <  1:998444/140‑1 (MQ=255)                              ccGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGCCAGGGTTTTAAGCTGAGGAATGAGTTGCTTgcg                                                                                                        >  2:909584/1‑140 (MQ=255)                              ccGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGCCAGGGTTTTAAGCTGAGGAATGAGTTGCTTgcg                                                                                                        >  2:666683/1‑140 (MQ=255)                              ccGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGCCAGGGTTTTAAGCTGAGGAATGAGTTGCTTgcg                                                                                                        >  1:1156491/1‑140 (MQ=255)                                       tcCTGCCCCAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGCCAGGGTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCcag                                                                                               <  2:973456/140‑1 (MQ=255)                                       tcCTGCCCCAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGCCAGGGTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCcag                                                                                               <  2:707746/140‑1 (MQ=255)                                       tcCTGCCCCAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGCCAGGGTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCcag                                                                                               <  2:169875/140‑1 (MQ=255)                                          tGCCCCAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGCCAGGGTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCcagcag                                                                                            <  2:1158455/140‑1 (MQ=255)                                              ccAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGCCAGGGTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGcgcc                                                                                        <  1:487839/140‑1 (MQ=255)                                               cAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGCCAGGGTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGcgccg                                                                                       <  1:269511/140‑1 (MQ=255)                                                 gCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGCCAGGGTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCa                                                                                     <  1:696723/140‑1 (MQ=255)                                                   gTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGCCAGGGTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAAc                                                                                   >  1:760561/1‑140 (MQ=255)                                                           cGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGCCAGGGTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCg                                                                           >  2:942776/1‑140 (MQ=255)                                                                       cAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGCCAGGGTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGa                                                               >  1:757958/1‑140 (MQ=255)                                                                         aGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGCCAGGGTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACa                                                             >  1:830238/1‑140 (MQ=255)                                                                          gTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGCCAGGGTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACAt                                                            >  1:887311/1‑140 (MQ=255)                                                                             aGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGCCAGGGTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGt                                                         >  1:299825/1‑140 (MQ=255)                                                                               tACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGCCAGGGTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTg                                                       <  2:830238/140‑1 (MQ=255)                                                                                   aCCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGCCAGGGTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAAc                                                   >  2:217322/1‑140 (MQ=255)                                                                                    ccAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGCCAGGGTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTgg                                                      >  1:440547/1‑136 (MQ=255)                                                                                    ccAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGCCAGGGTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTgg                                                      <  2:440547/136‑1 (MQ=255)                                                                                                  atCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGCCAGGGTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCTTTTGTTCa                                    >  2:589769/1‑140 (MQ=255)                                                                                                                gggATTTCTCATTCAGTGTCTGTGCCAGGGTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCTTTTGTTCACGTCGTCGTTAggg                      <  1:589769/140‑1 (MQ=255)                                                                                                                gggATTTCTCATTCAGTGTCTGTGCCAGGGTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCTTTTGTTCACGTCGTCGTTAggg                      >  2:564551/1‑140 (MQ=255)                                                                                                                   aTTTCTCATTCAGTGTCTGTGCCAGGGTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCTTTTGTTCACGTCGTCGTTAGGGTGa                   <  2:760561/140‑1 (MQ=255)                                                                                                                    tttCTCATTCAGTGTCTGTGCCAGGGTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCTTTTGTTCACGTCGTCGTTAGGGTGaa                  <  2:299825/140‑1 (MQ=255)                                                                                                                        tcATTCAGTGTCTGTGCCAGGGTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCTTTTGTTCACGTCGTCGTTAGGGTGAACttt              <  1:909584/140‑1 (MQ=255)                                                                                                                                tgtCTGTGCCAGGGTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCTTTTGTTCACGTCGTCGTTAGGGTGAACTTTACGTTCCa      >  1:293565/1‑140 (MQ=255)                                                                                                                                tgtCTGTGCCAGGGTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCTTTTGTTCACGTCGTCGTTAGGGTGAACTTTACGTTCCa      >  1:161990/1‑140 (MQ=255)                                                                                                                                  tCTGTGCCAGGGTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCTTTTGTTCACGTCGTCGTTAGGGTGAACTTTACGTTCCATc    >  1:205592/1‑140 (MQ=255)                                                                                                                                  tCTGTGCCAGGGTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCTTTTGTTCACGTCGTCGTTAGGGTGAACTTTACGTTCCATc    >  1:113840/1‑140 (MQ=255)                                                                                                                                    tgtgCCAGGGTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCTTTTGTTCACGTCGTCGTTAGGGTGAACTTTACGTTCCATccc  >  1:144651/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        TGAGATTATGCTCGAGCATCGCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGGTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCTTTTGTTCACGTCGTCGTTAGGGTGAACTTTACGTTCCATCCC  >  NC_000913/1685952‑1686222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑