Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,802,361	G→A	I70I (ATC→ATT)	thrS ←	threonine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,802,361	0	G	A	100.0%	188.7 / NA	53	I70I (ATC→ATT)	thrS	threonine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/23);  total (30/23)

GGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATCAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCTGGACCAATGTCC  >  NC_000913/1802231‑1802496                                                                                                                                   |                                                                                                                                        gggTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATAATCTCCAGa                                                                                                                                >  2:262769/1‑140 (MQ=255)     tAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATAATCTCCAGACctt                                                                                                                            >  2:1040068/1‑140 (MQ=255)      aaCGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATAATCTCCAGACcttc                                                                                                                           >  1:955910/1‑140 (MQ=255)      aaCGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATAATCTCCAGACcttc                                                                                                                           >  2:299188/1‑140 (MQ=255)       aCGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATAATCTCCAGACcttct                                                                                                                          >  2:300437/1‑140 (MQ=255)       aCGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATAATCTCCAGACcttct                                                                                                                          <  2:634100/140‑1 (MQ=255)        cGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATAATCTCCAGACcttctt                                                                                                                         >  1:634518/1‑140 (MQ=255)        cGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATAATCTCCAGACcttctt                                                                                                                         >  1:92963/1‑140 (MQ=255)           gCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATAATCTCCAGACCTTCTTCGt                                                                                                                      >  2:51211/1‑140 (MQ=255)              gTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATAATCTCCAGACCTTCTTCGTCtt                                                                                                                   <  2:625550/140‑1 (MQ=255)                 aaGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATAATCTCCAGACCTTCTTCGTCTTTgg                                                                                                                <  2:634518/140‑1 (MQ=255)                  aGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATAATCTCCAGACCTTCTTCGTCTTTGGc                                                                                                               <  1:825577/140‑1 (MQ=255)                    aTCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATAATCTCCAGACCTTCTTCGTCTTTGGCgg                                                                                                             <  1:299188/140‑1 (MQ=255)                    aTCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATAATCTCCAGACCTTCTTCGTCTTTGGCgg                                                                                                             <  2:92963/140‑1 (MQ=255)                         cgtcgtAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATAATCTCCAGACCTTCTTCGTCTTTgg                                                                                                                >  1:216624/1‑132 (MQ=255)                         cgtcgtAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATAATCTCCAGACCTTCTTCGTCTTTgg                                                                                                                <  2:216624/132‑1 (MQ=255)                         cgtcgtAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATAATCTCCAGACCTTCTTCGTCTTTGGCGGTAATg                                                                                                        <  1:300437/140‑1 (MQ=255)                         cgtcgtAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATAATCTCCAGACCTTCTTCGTCTTTGGCGGTAATg                                                                                                        >  1:724606/1‑140 (MQ=255)                             gtAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATAATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCg                                                                                                    >  2:1052417/1‑140 (MQ=255)                              taataaAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATAATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGa                                                                                                   <  2:724606/140‑1 (MQ=255)                                      aCCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATAATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTtgtg                                                                                           >  2:226716/1‑140 (MQ=255)                                            gTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATAATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGcgtcgt                                                                                     >  2:252475/1‑140 (MQ=255)                                            gTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATAATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGcgtcgt                                                                                     >  2:80385/1‑140 (MQ=255)                                                 tAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATAATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCa                                                                                <  1:80385/140‑1 (MQ=255)                                                   aCCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATAATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAAt                                                                              <  2:497481/140‑1 (MQ=255)                                                      gggCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATAATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAg                                                                           <  1:262769/140‑1 (MQ=255)                                                       ggCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATAATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGGTCGACAGTTGTGCGTCGTTTTCAATCAGa                                                                          >  1:916417/1‑140 (MQ=255)                                                       ggCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATAATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGcgt                                                                                        <  1:776877/126‑1 (MQ=255)                                                       ggCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATAATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGcgt                                                                                        >  2:776877/1‑126 (MQ=255)                                                       ggCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATAATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGa                                                                          >  2:1077362/1‑140 (MQ=255)                                                       ggCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATAATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGa                                                                          >  1:646978/1‑140 (MQ=255)                                                       ggCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATAATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGa                                                                          >  1:453325/1‑140 (MQ=255)                                                       ggCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATAATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGa                                                                          >  2:981564/1‑140 (MQ=255)                                                         ccGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATAATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATc                                                                        >  1:662645/1‑140 (MQ=255)                                                                  aTTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATAATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATc                                                               <  1:1052417/140‑1 (MQ=255)                                                                     ttGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATAATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATCAAc                                                            <  1:51211/140‑1 (MQ=255)                                                                           tGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATAATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATCAACCAGTTc                                                      >  2:780642/1‑140 (MQ=255)                                                                              ggCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATAATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATCAAcc                                                           >  2:286957/1‑132 (MQ=255)                                                                              ggCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATAATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATCAAcc                                                           <  1:286957/132‑1 (MQ=255)                                                                               gCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATAATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATCAACCAGTTCGCCa                                                  >  1:1167899/1‑140 (MQ=255)                                                                                         tttAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATAATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATCAACCAGTTCGCCATTAACGCGcc                                        >  2:756053/1‑140 (MQ=255)                                                                                            aaTCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATAATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATCAACCAGTTCGCCATTAACGCGCCCTg                                     <  2:646978/140‑1 (MQ=255)                                                                                            aaTCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATAATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATCAACCAGTTCGCCATTAACGCGCCCTg                                     <  2:916417/140‑1 (MQ=255)                                                                                                 cgTGCCCTAACAGGTGCGCACAGGAGTGACGAATAATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATCAACCAGTTCGCCATTAACGCGCCCTGCGATa                                >  2:667411/1‑140 (MQ=255)                                                                                                            aGGTGCGCACAGGAGTGACGAATAATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATCAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGc                     <  2:1167899/140‑1 (MQ=255)                                                                                                             gttGCGCACAGGAGTGACGAATAGTCTCCAGACCATCTTCGTCTTTGGCGGTAATGAGCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATCAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGcc                    <  2:949509/138‑1 (MQ=255)                                                                                                                gcgcACAGGAGTGACGAATAATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATCAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGc                     >  1:703702/1‑136 (MQ=255)                                                                                                                gcgcACAGGAGTGACGAATAATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATCAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGc                     <  2:703702/136‑1 (MQ=255)                                                                                                                    acaGGAGTGACGAATAATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATCAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCTg             >  1:1039183/1‑140 (MQ=255)                                                                                                                        gAGTGACGAATAATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATCAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGcc                    <  1:695360/129‑1 (MQ=255)                                                                                                                        gAGTGACGAATAATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATCAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGcc                    >  2:695360/1‑129 (MQ=255)                                                                                                                        gAGTGACGAATAATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATCAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCTGGAcc         >  1:845808/1‑140 (MQ=255)                                                                                                                               gAATAATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATCAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCTGGACCAATGTcc  <  2:662645/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                        GGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATCAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCTGGACCAATGTCC  >  NC_000913/1802231‑1802496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑