Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,831,911	T→C	I24M (ATA→ATG)	astC ←	succinylornithine transaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,831,911	0	T	C	100.0%	200.4 / NA	56	I24M (ATA→ATG)	astC	succinylornithine transaminase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/31);  total (25/31)

ACTCGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGTATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGTTAATAATAAGTAATGTTTGCGCTGTAA  >  NC_000913/1831776‑1832046                                                                                                                                        |                                                                                                                                        aCTCGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATaa                                                                                                                                     >  1:138497/1‑140 (MQ=255)           tcgttcAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCACCCGCGAAGTCAATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCgg                                                                                                                           >  2:813005/1‑140 (MQ=255)            cgttcAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGa                                                                                                                          <  1:954054/140‑1 (MQ=255)               tcAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCg                                                                                                                       <  2:1083216/140‑1 (MQ=255)               tcAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCg                                                                                                                       <  1:1169008/140‑1 (MQ=255)               tcAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCg                                                                                                                       >  2:334484/1‑140 (MQ=255)                   cgcTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTgaa                                                                                                                   >  2:618886/1‑140 (MQ=255)                   cgcTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTaaa                                                                                                                   <  2:10706/140‑1 (MQ=255)                   cgcTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTaaa                                                                                                                   <  1:813005/140‑1 (MQ=255)                   cgcTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTaaa                                                                                                                   <  2:38614/140‑1 (MQ=255)                   cgcTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGACATAAAGGGTGCCGGAGCGTaaa                                                                                                                   >  2:514512/1‑140 (MQ=255)                    gcTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAAc                                                                                                                  >  1:665204/1‑140 (MQ=255)                             aGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTatcat                                                                                                         >  1:214524/1‑140 (MQ=255)                               ttCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATcc                                                                                                       >  2:931583/1‑140 (MQ=255)                                       gcgcATGGCCCAGCGCGTTCACCGCAATGCCACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATtcatca                                                                                               >  2:726123/1‑140 (MQ=255)                                         gcATGGCCCAGCGCGTTCACCGCAATGCCACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCaaa                                                                                             >  1:243099/1‑140 (MQ=255)                                         gcATGGCCCAGCGCGTTCACCGCAATGCCACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCaaa                                                                                             >  1:433651/1‑140 (MQ=255)                                             ggCCCAGCGCGTTCACCGCAATGCCACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGttt                                                                                         <  1:1021234/140‑1 (MQ=255)                                                 cAGCGCGTTCACCGCAATGCCACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCAc                                                                                     <  2:998910/140‑1 (MQ=255)                                                   gcgcGTTCACCGCAATGCCACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCAcgc                                                                                   >  1:554169/1‑140 (MQ=255)                                                    cgcgTTCACCGCAATGCCACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCAcgcg                                                                                  >  1:826033/1‑140 (MQ=255)                                                       gTTCACCGCAATGCCACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTaa                                                                               <  1:334484/140‑1 (MQ=255)                                                       gTTCACCGCAATGCCACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTaa                                                                               <  2:937885/140‑1 (MQ=255)                                                        ttCACCGCAATGCCACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAAt                                                                              >  2:731742/1‑140 (MQ=255)                                                            ccGCAATGCCACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGc                                                                          <  1:777719/140‑1 (MQ=255)                                                                     cACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATa                                                                 <  1:896448/140‑1 (MQ=255)                                                                       cccGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGc                                                                          <  2:348573/129‑1 (MQ=255)                                                                       cccGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGc                                                                          >  1:348573/1‑129 (MQ=255)                                                                       cccGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGc                                                               <  1:731742/140‑1 (MQ=255)                                                                        ccGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCg                                                              <  2:665204/140‑1 (MQ=255)                                                                        ccGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCg                                                              <  1:1119370/140‑1 (MQ=255)                                                                          gcgAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGAc                                                            <  1:931583/140‑1 (MQ=255)                                                                          gcgAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGAc                                                            <  2:1011601/140‑1 (MQ=255)                                                                          gcgAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGACATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGAc                                                            <  1:514512/140‑1 (MQ=255)                                                                            gAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGACct                                                          <  1:702400/140‑1 (MQ=255)                                                                              aGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGc                                                               >  2:1074432/1‑133 (MQ=255)                                                                              aGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGc                                                               <  1:1074432/133‑1 (MQ=255)                                                                               gTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGACCTCTa                                                       <  2:554169/140‑1 (MQ=255)                                                                                      taCTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTaaa                                                <  2:243099/140‑1 (MQ=255)                                                                                         tctTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTGAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTAaataa                                             <  1:618886/140‑1 (MQ=255)                                                                                          ctTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTAaataat                                            >  1:235115/1‑140 (MQ=255)                                                                                          ctTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTAaataat                                            >  1:537125/1‑140 (MQ=255)                                                                                            ttCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTc                                          >  1:53578/1‑140 (MQ=255)                                                                                              ccccTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAGTTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGt                                        >  1:49274/1‑140 (MQ=255)                                                                                                   gctgATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGttatt                                    >  1:733558/1‑139 (MQ=255)                                                                                                   gctgATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGttatt                                    <  2:733558/139‑1 (MQ=255)                                                                                                     tgATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGttatttat                                 <  2:826033/140‑1 (MQ=255)                                                                                                     tgATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGttatttat                                 <  2:138497/140‑1 (MQ=255)                                                                                                       aTCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTatat                               >  2:246000/1‑140 (MQ=255)                                                                                                       aTCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTatat                               >  2:849010/1‑140 (MQ=255)                                                                                                       aTCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTatat                               >  1:722573/1‑140 (MQ=255)                                                                                                         cccACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGt                             <  1:695992/140‑1 (MQ=255)                                                                                                              aaGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGTtaata                        <  1:849010/140‑1 (MQ=255)                                                                                                                 cgcgAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGTtaataata                     >  2:83315/1‑140 (MQ=255)                                                                                                                                 gTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGTTAATAATAAGTAATGTTTGCGCTg     <  2:214524/140‑1 (MQ=255)                                                                                                                                    ccGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGTTAATAATAAGTAATGTTTGCGCTGTaa  <  1:957430/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        ACTCGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGTATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGTTAATAATAAGTAATGTTTGCGCTGTAA  >  NC_000913/1831776‑1832046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑