Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,837,450	G→A	W80* (TGG→TAG)	ynjC →	putative ABC transporter membrane subunit YnjC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,837,450	0	G	A	100.0%	254.2 / NA	73	W80* (TGG→TAG)	ynjC	putative ABC transporter membrane subunit YnjC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/35);  total (38/35)

GCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTGGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACT  >  NC_000913/1837314‑1837586                                                                                                                                         |                                                                                                                                         gCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGcc                                                                                                                                       <  2:1017154/140‑1 (MQ=255)  cTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCt                                                                                                                                      >  2:1074952/1‑140 (MQ=255)  cTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCt                                                                                                                                      >  1:59336/1‑140 (MQ=255)  cTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCt                                                                                                                                      >  2:615293/1‑140 (MQ=255)  cTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCt                                                                                                                                      >  1:365583/1‑140 (MQ=255)     tGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACTGTCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTggg                                                                                                                                   >  2:809398/1‑140 (MQ=255)                 acTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAg                                                                                                                       >  1:1716/1‑140 (MQ=255)                  cTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGTGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGc                                                                                                                      <  2:292361/140‑1 (MQ=255)                   tGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCg                                                                                                                     >  2:685238/1‑140 (MQ=255)                    ggCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGt                                                                                                                    <  1:615293/140‑1 (MQ=255)                         gCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTAtgtg                                                                                                               <  2:863888/140‑1 (MQ=255)                               tttGCCGATCCACAGTTACCGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCg                                                                                                         <  2:59336/140‑1 (MQ=255)                                     gATCCACAGTTACCGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGcc                                                                                                   >  2:917954/1‑140 (MQ=255)                                     gATCCACAGTTACCGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGcc                                                                                                   >  2:483102/1‑140 (MQ=255)                                       tCCACAGTTACCGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGt                                                                                                 >  1:583564/1‑140 (MQ=255)                                             gTTACCGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGgctgc                                                                                           <  1:381484/140‑1 (MQ=255)                                                aCCGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGgctgct                                                                                          <  2:686723/138‑1 (MQ=255)                                                aCCGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGgctgct                                                                                          >  1:686723/1‑138 (MQ=255)                                                aCCGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCg                                                                                        >  1:230759/1‑140 (MQ=255)                                                aCCGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCg                                                                                        <  2:595596/140‑1 (MQ=255)                                                 ccGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTc                                                                                                       <  2:677903/124‑1 (MQ=255)                                                 ccGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTc                                                                                                       >  1:677903/1‑124 (MQ=255)                                                 ccGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGc                                                                                       <  2:583564/140‑1 (MQ=255)                                                 ccGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGc                                                                                       <  2:365583/140‑1 (MQ=255)                                                  cGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGcc                                                                                      >  2:513271/1‑140 (MQ=255)                                                  cGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGcc                                                                                      >  1:155514/1‑140 (MQ=255)                                                    cAGGCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCAt                                                                                    <  2:1716/140‑1 (MQ=255)                                                      ggCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGgctgct                                                                                          <  1:667243/132‑1 (MQ=255)                                                      ggCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGgctgct                                                                                          >  2:667243/1‑132 (MQ=255)                                                      ggCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGgctgct                                                                                          >  1:246983/1‑132 (MQ=255)                                                      ggCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGgctgct                                                                                          <  2:246983/132‑1 (MQ=255)                                                      ggCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTc                                                                                  >  2:729843/1‑140 (MQ=255)                                                      ggCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTc                                                                                  >  2:491035/1‑140 (MQ=255)                                                      ggCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCCGCTGGTGGTTGTGGCGCTGTAGCCTGGGCCGAAAAGGCCGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCCTTc                                                                                  >  2:186545/1‑140 (MQ=255)                                                             cTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCAtgt                                                                           <  1:1074952/140‑1 (MQ=255)                                                               ggCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTgg                                                                         <  1:483102/140‑1 (MQ=255)                                                                 cAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTgg                                                                         <  1:1160570/138‑1 (MQ=255)                                                                 cAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTgg                                                                         >  2:1160570/1‑138 (MQ=255)                                                                 cAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCt                                                                       >  2:511020/1‑140 (MQ=255)                                                                   aCGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCttt                                                                     >  2:380418/1‑140 (MQ=255)                                                                   aCGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCttt                                                                     >  2:187152/1‑140 (MQ=255)                                                                     gCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTg                                                                   <  2:1121243/140‑1 (MQ=255)                                                                            tCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAg                                                            <  1:685238/140‑1 (MQ=255)                                                                              gACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAgcg                                                          <  1:380418/140‑1 (MQ=255)                                                                              gACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAgcg                                                          <  2:155514/140‑1 (MQ=255)                                                                               aCAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAgcgc                                                         >  1:774217/1‑140 (MQ=255)                                                                                   ccATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCtt                                                     <  1:511020/140‑1 (MQ=255)                                                                                   ccATCGCGGCGGTCGGGGCATTGTTGATTG‑CCTGCTGGGGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTc                                                    >  1:660662/1‑140 (MQ=255)                                                                                          ggcggTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGctct                                              <  1:186545/140‑1 (MQ=255)                                                                                              gTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGc                                          >  1:359158/1‑140 (MQ=255)                                                                                                   ggCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACg                                     <  2:709565/140‑1 (MQ=255)                                                                                                   ggCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACg                                     <  1:491035/140‑1 (MQ=255)                                                                                                        tgttgATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAggg                                <  1:809398/140‑1 (MQ=255)                                                                                                        tgttgATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAggg                                <  2:323200/140‑1 (MQ=255)                                                                                                        tgttgATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAggg                                <  2:774217/140‑1 (MQ=255)                                                                                                        tgttgATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAggg                                <  1:187152/140‑1 (MQ=255)                                                                                                         gttgATTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGgc                               <  2:359158/140‑1 (MQ=255)                                                                                                             aTTGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGgctgct                           >  1:1098165/1‑140 (MQ=255)                                                                                                              ttGCCCTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCtt                          >  1:828760/1‑140 (MQ=255)                                                                                                                 cccTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTAt                       >  2:477296/1‑140 (MQ=255)                                                                                                                 cccTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTAt                       >  2:1115670/1‑140 (MQ=255)                                                                                                                  ccTGCTGGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATg                      >  2:1163332/1‑140 (MQ=255)                                                                                                                     gctgGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACt                   <  1:726864/140‑1 (MQ=255)                                                                                                                      ctgGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTa                  >  1:361326/1‑140 (MQ=255)                                                                                                                      ctgGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTa                  >  2:695670/1‑140 (MQ=255)                                                                                                                       tgGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTAt                 <  1:4835/140‑1 (MQ=255)                                                                                                                       tgGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTAt                 <  2:642354/140‑1 (MQ=255)                                                                                                                       tgGTGATTGTGGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTAt                 >  1:278759/1‑140 (MQ=255)                                                                                                                              tgtgGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTccc            >  2:1047502/1‑138 (MQ=255)                                                                                                                              tgtgGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTccc            <  1:1047502/138‑1 (MQ=255)                                                                                                                                tgGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTAt        >  1:523723/1‑140 (MQ=255)                                                                                                                                tgGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTAt        >  1:658512/1‑140 (MQ=255)                                                                                                                                tgGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGATGACGGAGGGCTGCTTTATGACTATTTCCCGTAt        >  2:1124484/1‑140 (MQ=255)                                                                                                                                tgGCGCTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACAAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTAt        >  2:253252/1‑140 (MQ=255)                                                                                                                                    gcTGTAGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCa    <  2:1098165/140‑1 (MQ=255)                                                                                                                                      tgtaGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACt  <  2:660662/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         GCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTGGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACT  >  NC_000913/1837314‑1837586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑