Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,940,639	C→T	G333S (GGT→AGT)	mepM ←	peptidoglycan DD‑endopeptidase MepM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,940,639	0	C	T	100.0%	182.6 / NA	51	G333S (GGT→AGT)	mepM	peptidoglycan DD‑endopeptidase MepM
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/26);  total (25/26)

CGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTAGACGAGATACGGAACTGTTTCGCCGTC  >  NC_000913/1940504‑1940772                                                                                                                                        |                                                                                                                                      cGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACTGTCa                                                                                                                                   <  2:702491/140‑1 (MQ=255)     aCGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACTGTCACCCa                                                                                                                               >  2:77744/1‑140 (MQ=255)            aCCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACTGTCACCCACTGAAAg                                                                                                                        >  1:86883/1‑140 (MQ=255)              cTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACTGTCACCCACTGAAAGCa                                                                                                                      <  1:126344/140‑1 (MQ=255)              cTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACTGTCACCCACTGAAAGCa                                                                                                                      <  1:484070/140‑1 (MQ=255)                tCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACTGt                                                                                                                                     >  1:1092517/1‑123 (MQ=255)                    tCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACTGTCACCCACTGAAAGCACTGGCg                                                                                                                >  1:16446/1‑140 (MQ=255)                    tCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACTGTCACCCACTGAAAGCACTGGCg                                                                                                                <  1:253897/140‑1 (MQ=255)                    tCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACTGTCACCCACTGAAAGCACTGGCg                                                                                                                >  2:253897/1‑140 (MQ=255)                    tCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACTGTCACCCACTGAAAGCACTGGCg                                                                                                                >  1:296269/1‑140 (MQ=255)                    tCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACTGTCACCCACTGAAAGCACTGGCg                                                                                                                <  1:372967/140‑1 (MQ=255)                    tCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACTGTCACCCACTGAAAGCACTGGCg                                                                                                                >  1:534343/1‑140 (MQ=255)                                    aTCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACTGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGc                                                                                                >  2:897753/1‑140 (MQ=255)                                     tCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACTGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCg                                                                                               <  2:652025/140‑1 (MQ=255)                                     tCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACTGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCg                                                                                               <  1:786817/140‑1 (MQ=255)                                       ttGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACTGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGaa                                                                                             <  1:487816/140‑1 (MQ=255)                                            cAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACTGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCaa                                                                                        <  1:77744/140‑1 (MQ=255)                                             aaGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACTGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAac                                                                                       >  1:1001111/1‑140 (MQ=255)                                                 gCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACTGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACct                                                                                   <  2:311388/140‑1 (MQ=255)                                                   atatAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACTGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACctct                                                                                 <  1:255999/140‑1 (MQ=255)                                                      taACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACTGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCtgtg                                                                              <  2:1001111/140‑1 (MQ=255)                                                         cgcgTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACTGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTgtgg                                                                           <  1:602990/140‑1 (MQ=255)                                                             tggtgTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACTGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCa                                                                       >  1:403123/1‑140 (MQ=255)                                                                    gCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACTGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGAc                                                                <  2:16446/140‑1 (MQ=255)                                                                    gCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACTGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGAc                                                                <  2:534343/140‑1 (MQ=255)                                                                      tGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACTGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCg                                                              <  2:296269/140‑1 (MQ=255)                                                                        cGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACTGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGt                                                            >  2:562990/1‑140 (MQ=255)                                                                        cGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACTGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGt                                                            >  2:231490/1‑140 (MQ=255)                                                                          aCCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACTGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGt                                                            >  1:7511/1‑138 (MQ=255)                                                                          aCCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACTGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGt                                                            <  2:7511/138‑1 (MQ=255)                                                                           ccATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACTGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCAc                                                         <  1:504858/140‑1 (MQ=255)                                                                                    aTAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACTGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGt                                                <  2:86883/140‑1 (MQ=255)                                                                                     tAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACTGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGAc                                                                >  1:397365/1‑123 (MQ=255)                                                                                     tAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACTGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGAc                                                                <  2:397365/123‑1 (MQ=255)                                                                                      aGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACTGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCAc                                                         <  1:688629/129‑1 (MQ=255)                                                                                      aGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACTGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCAc                                                         >  2:688629/1‑129 (MQ=255)                                                                                               ataaCCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACTGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGt                                     <  1:414183/140‑1 (MQ=255)                                                                                               ataaCCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACTGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGt                                     <  1:897753/140‑1 (MQ=255)                                                                                                taaCCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACTGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGtt                                    >  1:476482/1‑140 (MQ=255)                                                                                                      gctgcGCCACTGCGTTTGGCAACCACCACTTCACTGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAAtt                              >  2:180834/1‑140 (MQ=255)                                                                                                       ctgcGCCACTGCGTTTGGCAACCACCACTTCACTGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTa                             >  2:497791/1‑140 (MQ=255)                                                                                                        tgcGCCACTGCGTTTGGCAACCACCACTTCACTGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTacgacg                                          <  2:211764/126‑1 (MQ=255)                                                                                                        tgcGCCACTGCGTTTGGCAACCACCACTTCACTGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTacgacg                                          >  1:211764/1‑126 (MQ=255)                                                                                                          cgcCACTGCGTTTGGCAACCACCACTTCACTGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTAGAc                          >  1:1074127/1‑140 (MQ=255)                                                                                                                 gCGTTTGGCAACCACCACTTCACTGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAAt                               >  2:514378/1‑128 (MQ=255)                                                                                                                 gCGTTTGGCAACCACCACTTCACTGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAAt                               <  1:514378/128‑1 (MQ=255)                                                                                                                    tttGGCAACCACCACTTCACTGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTAGACGAGATACGGa                >  2:862090/1‑140 (MQ=255)                                                                                                                     ttGGCAACCACCACTTCACTGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTAGACGAGATACGGaa               <  2:1074127/140‑1 (MQ=255)                                                                                                                       ggCAACCACCACTTCACTGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTAGACGAGATACGGAACt             >  1:814371/1‑140 (MQ=255)                                                                                                                            ccaccaCTTCACTGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTAGACGAGATACGGAACTGTTTc        >  1:920934/1‑140 (MQ=255)                                                                                                                                  cTTCACTGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTAGACGAGATACGGAACTGTTTCGCCGTc  >  2:369080/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                      CGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTAGACGAGATACGGAACTGTTTCGCCGTC  >  NC_000913/1940504‑1940772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑