Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,185,457	G→A	intergenic (‑156/+67)	thiM ← / ← rcnR	hydroxyethylthiazole kinase/DNA‑binding transcriptional repressor RcnR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,185,457	0	G	A	100.0%	215.8 / NA	61	intergenic (‑156/+67)	thiM/rcnR	hydroxyethylthiazole kinase/DNA‑binding transcriptional repressor RcnR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/30);  total (31/30)

AAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCATCC  >  NC_000913/2185324‑2185590                                                                                                                                      |                                                                                                                                      aagaagCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTa                                                                                                                                 >  1:892802/1‑140 (MQ=255)            aCCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAgcgc                                                                                                                      >  1:280787/1‑140 (MQ=255)            aCCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAgcgc                                                                                                                      >  2:1171428/1‑140 (MQ=255)               tggtggTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCAcg                                                                                                                   <  2:918584/140‑1 (MQ=255)                 gtggtCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCAcgcg                                                                                                                 >  1:465740/1‑140 (MQ=255)                  tggtCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGt                                                                                                                >  2:676570/1‑140 (MQ=255)                       cGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTaa                                                                                                           <  2:465740/140‑1 (MQ=255)                               cccTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGaa                                                                                                   >  1:786345/1‑140 (MQ=255)                               cccTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGaa                                                                                                   >  1:116456/1‑140 (MQ=255)                                   actcTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAAc                                                                                               >  2:557226/4‑140 (MQ=255)                                   aCGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAAc                                                                                               <  1:362340/140‑1 (MQ=255)                                     gCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACgg                                                                                             <  2:288971/140‑1 (MQ=255)                                     gCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACgg                                                                                             <  1:299799/140‑1 (MQ=255)                                      cTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGc                                                                                            <  2:892802/140‑1 (MQ=255)                                         gCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTaa                                                                                                           <  2:898021/122‑1 (MQ=255)                                         gCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTaa                                                                                                           >  1:898021/1‑122 (MQ=255)                                         gCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGt                                                                                         >  2:901099/1‑140 (MQ=255)                                         gCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGt                                                                                         >  2:977016/1‑140 (MQ=255)                                               tCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTa                                                                                     <  1:668204/138‑1 (MQ=255)                                               tCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTa                                                                                     >  2:668204/1‑138 (MQ=255)                                               tCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACa                                                                                   >  1:855465/1‑140 (MQ=255)                                                ccAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACaa                                                                                  <  2:279149/140‑1 (MQ=255)                                                   gATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATcg                                                                               <  2:116456/140‑1 (MQ=255)                                                         gTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTtaa                                                                         >  1:1030097/1‑140 (MQ=255)                                                         gTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTtaa                                                                         >  2:726412/1‑140 (MQ=255)                                                         gTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTtaa                                                                         >  1:764855/1‑140 (MQ=255)                                                         gTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTtaa                                                                         >  2:822054/1‑140 (MQ=255)                                                                  gTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAattatt                                                                <  1:410955/140‑1 (MQ=255)                                                                   tATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGTGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTAttt                                                               >  1:748571/1‑140 (MQ=255)                                                                   tATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTAttt                                                               <  2:855465/140‑1 (MQ=255)                                                                   tATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTAttt                                                               >  1:441270/1‑140 (MQ=255)                                                                     tttCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGa                                                             <  2:280787/140‑1 (MQ=255)                                                                        ctcAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGatat                                                          <  1:776234/140‑1 (MQ=255)                                                                          cAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGatatat                                                        <  1:557226/140‑1 (MQ=255)                                                                          cAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGGCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGatatat                                                        >  2:788738/1‑140 (MQ=255)                                                                            gCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATaaa                                                                     >  2:717161/1‑125 (MQ=255)                                                                            gCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATaaa                                                                     <  1:717161/125‑1 (MQ=255)                                                                            gCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGa                                                      >  1:974872/1‑140 (MQ=255)                                                                              cTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAAt                                                    <  1:901099/140‑1 (MQ=255)                                                                                tCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATcc                                                  >  1:106412/1‑140 (MQ=255)                                                                                tCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATcc                                                  >  1:739046/1‑140 (MQ=255)                                                                                  aCGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAg                                                <  2:1030097/140‑1 (MQ=255)                                                                                     cAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCAc                                             <  1:822054/140‑1 (MQ=255)                                                                                      aGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCAcc                                            <  2:441270/140‑1 (MQ=255)                                                                                          gggCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCa                                        >  1:475907/1‑140 (MQ=255)                                                                                          gggCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCa                                        <  2:764855/140‑1 (MQ=255)                                                                                             cACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGaa                                     >  2:385961/1‑140 (MQ=255)                                                                                               ccccGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACg                                   <  2:475907/140‑1 (MQ=255)                                                                                               ccccGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACg                                   <  2:786345/140‑1 (MQ=255)                                                                                                cccGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGa                                  <  1:593147/140‑1 (MQ=255)                                                                                                  cGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCa                                        >  2:223192/1‑132 (MQ=255)                                                                                                  cGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCa                                        <  1:223192/132‑1 (MQ=255)                                                                                                  cGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACa                                >  1:547649/1‑140 (MQ=255)                                                                                                    aGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATc                              <  1:156503/140‑1 (MQ=255)                                                                                                      tCGTTTGGTTGCGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCa                            >  2:1005864/1‑140 (MQ=255)                                                                                                                 cGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCa                 <  1:977016/140‑1 (MQ=255)                                                                                                                 cGATGATAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCa                 <  2:478895/140‑1 (MQ=255)                                                                                                                     gatAAATCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGtt             <  1:270206/140‑1 (MQ=255)                                                                                                                           tCGCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGTTTTAGCt       >  2:1144787/1‑140 (MQ=255)                                                                                                                             gCAGAGGAGAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCa     <  1:676570/140‑1 (MQ=255)                                                                                                                                gaggagAATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCATcc  >  2:764528/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                      AAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCATCC  >  NC_000913/2185324‑2185590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑