Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	214,929	A→G	T33A (ACC→GCC)	arfB →	peptidyl‑tRNA hydrolase, ribosome rescue factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	214,929	0	A	G	100.0%	288.6 / NA	82	T33A (ACC→GCC)	arfB	peptidyl‑tRNA hydrolase, ribosome rescue factor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/46);  total (36/46)

TAATCTGGAAGTGAACTTAACCGCCTGGCAACAACCTTCATGATTGTGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGACCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCGCCAGCCATCATTTGATCAGCAGTGATGGCGTGATTGTCATTAAGGCACAGGAATACCG  >  NC_000913/214794‑215065                                                                                                                                        |                                                                                                                                         taatCTGGAAGTGAACTTAACCGCCTGGCAACAACCTTCATGATTGTGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTc                                                                                                                                      <  2:801603/140‑1 (MQ=255)  aatCTGGAAGTGAACTTAACCGCCTGGCAACAACCTTCATGATTGTGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCa                                                                                                                                     <  1:374703/140‑1 (MQ=255)  aatCTGGAAGTGAACTTAACCGCCTGGCAACAACCTTCATGATTGTGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCa                                                                                                                                     >  2:223327/1‑140 (MQ=255)    tCTGGAAGTGAACTTAACCGCCTGGCAACAACCTTCATGATTGTGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAAc                                                                                                                                   >  2:954941/1‑140 (MQ=255)    tCTGGAAGTGAACTTAACCGCCTGGCAACAACCTTCATGATTGTGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAAc                                                                                                                                   >  2:735038/1‑140 (MQ=255)    tCTGGAAGTGAACTTAACCGCCTGGCAACAACCTTCATGATTGTGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAAc                                                                                                                                   <  2:337781/140‑1 (MQ=255)    tCTGAAAGTAACCTAAACCGCCTGGCAACAACCTTCATGATTGTGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAAc                                                                                                                                   <  2:293480/140‑1 (MQ=255)         aaGTGAACTTAACCGCCTGGCAACAACCTTCATGATTGTGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTa                                                                                                                              >  1:958024/1‑140 (MQ=255)           gTGAACTTAACCGCCTGGCAACAACCTTCATGATTGTGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTAtt                                                                                                                            >  2:1058348/1‑140 (MQ=255)              aaCTTAACCGCCTGGCAACAACCTTCATGATTGTGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCAt                                                                                                                         <  2:510563/140‑1 (MQ=255)              aaCTTAACCGCCTGGCAACAACCTTCATGATTGTGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCAt                                                                                                                         >  1:99172/1‑140 (MQ=255)                cTTAACCGCCTGGCAACACCCTTCATGATTGTGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCt                                                                                                                       <  2:655448/140‑1 (MQ=255)                  tacccGCCTGGCAACAACCTTCATGATTGTGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGc                                                                                                                     <  1:736432/137‑1 (MQ=255)                           ggCAACAACCTTCATGATTGTGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACa                                                                                                            >  1:505879/1‑140 (MQ=255)                           ggCAACAACCTTCATGATTGTGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACa                                                                                                            >  1:380716/1‑140 (MQ=255)                                       cATGATTGTGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGGGTTTTGACATTCGGGCGTCCa                                                                                                >  1:867884/1‑140 (MQ=255)                                       cATGATTGTGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCa                                                                                                <  2:505879/140‑1 (MQ=255)                                       cATGATTGTGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCa                                                                                                >  2:1097320/1‑140 (MQ=255)                                       cATGATTGTGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCa                                                                                                >  2:831019/1‑140 (MQ=255)                                       cATGATTGTGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCa                                                                                                >  1:387892/1‑140 (MQ=255)                                          gATTGTGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGcc                                                                                             <  1:841299/140‑1 (MQ=255)                                             tgtgATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGc                                                                                          >  2:290653/1‑140 (MQ=255)                                             tgtgATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGc                                                                                          >  2:692719/1‑140 (MQ=255)                                             tgtgATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGc                                                                                          <  2:1121338/140‑1 (MQ=255)                                              gtgATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGcc                                                                                         >  2:16236/1‑140 (MQ=255)                                               tgATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCa                                                                                        <  2:99172/140‑1 (MQ=255)                                               tgATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCa                                                                                        >  2:402087/1‑140 (MQ=255)                                                   ttCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGt                                                                                    <  1:402087/140‑1 (MQ=255)                                                      ccGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTAtt                                                                                 >  2:130187/1‑140 (MQ=255)                                                      ccGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTAtt                                                                                 >  2:205942/1‑140 (MQ=255)                                                       cGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGc                                                                                          >  2:1123572/1‑130 (MQ=255)                                                       cGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGc                                                                                          <  1:1123572/130‑1 (MQ=255)                                                            tGTTGCTATTCCCGATGGTGGGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAag                                                                           <  1:205942/140‑1 (MQ=255)                                                            tGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAag                                                                           <  1:130187/140‑1 (MQ=255)                                                               tGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGc                                                                        >  2:1082607/1‑140 (MQ=255)                                                                       ccGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTc                                                                <  1:268848/140‑1 (MQ=255)                                                                           tGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTcgccg                                                            >  2:136485/1‑140 (MQ=255)                                                                           tGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTcgccg                                                            <  1:16236/140‑1 (MQ=255)                                                                              tGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCgcca                                                         >  1:1001153/1‑140 (MQ=255)                                                                              tGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCgcca                                                         >  1:97311/1‑140 (MQ=255)                                                                              tGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCgcca                                                         >  1:493590/1‑140 (MQ=255)                                                                              tGAGCTTGAGATCACCGCCATTCGTGCGCAGAGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCgcca                                                         >  2:408268/1‑140 (MQ=255)                                                                                aGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCgccagc                                                       >  1:1017578/1‑140 (MQ=255)                                                                                 gCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCgccagcc                                                      <  2:958024/140‑1 (MQ=255)                                                                                   ttGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCGCCAGCcat                                                    <  1:1005264/140‑1 (MQ=255)                                                                                      agaTCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGGGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCGCCAGCcatcat                                                 <  2:867884/140‑1 (MQ=255)                                                                                      agaTCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCGCCAGCcatcat                                                 <  1:136485/140‑1 (MQ=255)                                                                                      agaTCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCGCCAGCcatcat                                                 <  1:1097320/140‑1 (MQ=255)                                                                                      agaTCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCGCCAGCcatcat                                                 <  2:387892/140‑1 (MQ=255)                                                                                      agaTCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCGCCAGCcatcat                                                 <  1:12028/140‑1 (MQ=255)                                                                                      agaTCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCGCCAGCcatcat                                                 <  1:735038/140‑1 (MQ=255)                                                                                       gaTCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGCTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCGCCAGCCATCAtt                                                >  2:786107/1‑140 (MQ=255)                                                                                          cACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCGCCAGCCATCATTTGa                                             <  1:489890/140‑1 (MQ=255)                                                                                           aCCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTAACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCGCCAGCCATCATTTGAt                                            >  1:458381/1‑140 (MQ=255)                                                                                            ccGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCGCCAGCCATCATTTGATc                                           >  2:855866/1‑140 (MQ=255)                                                                                               ccATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCGCCAGCCATCATTTGATc                                           <  1:1129283/137‑1 (MQ=255)                                                                                               ccATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCGCCAGCCATCATTTGATc                                           >  2:1129283/1‑137 (MQ=255)                                                                                                  ttCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCGCCAGCCATCATTTGATCAGCAGt                                     <  1:1168071/140‑1 (MQ=255)                                                                                                    cGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCGCCAGCCATCATTTGATCAGCAGTGa                                   <  1:954941/140‑1 (MQ=255)                                                                                                       gcgcAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCGCCAGCCATCATTTGa                                             <  2:1141696/127‑1 (MQ=255)                                                                                                       gcgcAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCGCCAGCCATCATTTGa                                             >  1:1141696/1‑127 (MQ=255)                                                                                                       gcgcAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCGCCAGCCATCATTTGATCAGCAGTGATgg                                >  2:243761/1‑140 (MQ=255)                                                                                                        cgcAGGGCGCGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCGCCAGCCATCATTTGATCAGCAGTGATGGc                               <  1:1056945/140‑1 (MQ=255)                                                                                                              gcgcGGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCGCCAGCCATCATTTGATCAGCAGTGATGGCGTGAtt                         <  1:22437/140‑1 (MQ=255)                                                                                                               cgcgGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCGCCAGCCATCATTTGATCAGCAGTGATGGCg                              >  2:319746/1‑134 (MQ=255)                                                                                                               cgcgGGCGGGCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCGCCAGCCATCATTTGATCAGCAGTGATGGCg                              <  1:319746/134‑1 (MQ=255)                                                                                                                 cgggcgggCAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCGCCAGCCATCATTTGATCAGCAGTGATGGCGTGATTGTc                      >  1:114827/1‑140 (MQ=255)                                                                                                                  gggcgggcAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCGCCAGCCATCATTTGATCAGCAGTGATGGCGTGATTGTCa                     <  1:1058348/140‑1 (MQ=255)                                                                                                                   ggcgggcAGCATGTTAATAAGGCCTCAACGGGTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCGCCAGGCATCATTTGATCAGCAGTGATGGCGTGATTGTCAt                    <  1:408268/140‑1 (MQ=255)                                                                                                                   ggcgggcAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCGCCAGCCATCATTTGATCAGCAGTGATGGCGTGATTGTCAt                    <  2:493590/140‑1 (MQ=255)                                                                                                                    gcgggcAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCGCCAGCCATCATTTGATCAGCAGTGATGGCGTGATTGTCAtt                   <  2:114827/140‑1 (MQ=255)                                                                                                                     cgggcAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCGCCAGCCATCATTTGATCAGCAGTGATGGCGTGATTGTCATTa                  <  1:223327/140‑1 (MQ=255)                                                                                                                      gggcAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCGCCAGCCATCATTTGATCAGCAGTGATGGCGTGATTGTCATTaa                 <  1:1064429/140‑1 (MQ=255)                                                                                                                       ggcAGCATGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCGCCAGCCATCATTTGATCAGCAGTGATGGCGTGATTGTCATTAAg                >  2:403977/1‑140 (MQ=255)                                                                                                                        gcagcaTGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTGCAAAGAGCGTCTGCTCGCCGCCAGCCATCATTTGATCAGCAGTGATGGCGTGATTGTCATTAAgg               <  1:290653/140‑1 (MQ=255)                                                                                                                          agcaTGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTAACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCGCCAGCCATCATTTGATCAGCAGTGATGGCGTGATTGTCATTAAGGca             <  2:458381/140‑1 (MQ=255)                                                                                                                           gcaTGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCGCCAGCCATCATTTGATCAGCAGTGATGGCGTGATTGTCATTAAGGcac            <  2:380716/140‑1 (MQ=255)                                                                                                                           gcaTGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCGCCAGCCATCATTTGATCAGCAGTGATGGCGTGATTGTCATTAAGGcac            <  1:403977/140‑1 (MQ=255)                                                                                                                             aTGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCGCCAGCCATCATTTGATCAGCAGTGATGGCGTGATTGTCATTAAGGCACAg          <  2:97311/140‑1 (MQ=255)                                                                                                                              tGTTAATAAGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCGCCAGCCATCATTTGATCAGCAGTGATGGCGTGATTGTCATTAAGGCACAgg         <  1:217021/140‑1 (MQ=255)                                                                                                                                  aataaGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCGCCAGCCATCATTTGATCAGCAGTGATGGCGTGATTGTCATTAAGGCACAGGAATa     >  1:904896/1‑140 (MQ=255)                                                                                                                                     aaGGCCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCGCCAGCCATCATTTGATCAGCAGTGATGGCGTGATTGTCATTAAGGCACAGGAATACCg  <  1:407368/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         TAATCTGGAAGTGAACTTAACCGCCTGGCAACAACCTTCATGATTGTGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGACCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAGAGCGTCTGCTCGCCGCCAGCCATCATTTGATCAGCAGTGATGGCGTGATTGTCATTAAGGCACAGGAATACCG  >  NC_000913/214794‑215065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑