Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,219,960	T→C	N677S (AAC→AGC)	bglX ←	beta‑D‑glucoside glucohydrolase, periplasmic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,219,960	0	T	C	100.0%	233.5 / NA	66	N677S (AAC→AGC)	bglX	beta‑D‑glucoside glucohydrolase, periplasmic
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/31);  total (35/31)

GTCTGAGTTTCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGCGACTCATGGAAGCCGTCACATCCTGCAAGTACATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGTTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGAAAGTTTCACATCAGAGACGGTGAAAGTGGTGTAGCTCAGCCCATAGCCGAACGGATACAACGCCCCGTTAGCTTCAT  >  NC_000913/2219831‑2220092                                                                                                                                  |                                                                                                                                     gTCTGAGTTTCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGCGACTCATGGAAGCCGTCACATCCTGCAAGTACATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTg                                                                                                                            >  2:1045952/1‑140 (MQ=255)   cTGAGTTTCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGCGACTCATGGAAGCCGTCACATCCTGCAAGTACATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCa                                                                                                                          <  1:138367/140‑1 (MQ=255)     gAGTTTCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGCGACTCATGGAAGCCGTCACATCCTGCAAGTACATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACg                                                                                                                        >  2:1027815/1‑140 (MQ=255)     gAGTTTCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGCGACTCATGGAAGCCGTCACATCCTGCAAGTACATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACg                                                                                                                        >  1:197818/1‑140 (MQ=255)     gAGTTTCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGCGACTCATGGAAGCCGTCACATCCTGCAAGTACATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACg                                                                                                                        >  1:332963/1‑140 (MQ=255)        tttCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGCGACTCATGGAAGCCGTCACATCCTGCAAGTACATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTg                                                                                                                     >  2:605909/1‑140 (MQ=255)          tCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGCGACTCATGGAAGCCGTCACATCCTGCAAGTACATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGc                                                                                                                   <  1:647778/140‑1 (MQ=255)             cccGGTTTCAGGGTGATTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGCGACTCATGGAAGCCGTCACATCCTGCAAGTACATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGt                                                                                                                >  1:613873/1‑140 (MQ=255)                          tGATTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGCGACTCATGGAAGCCGTCACATCCTGCAAGTACATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCa                                                                                                   <  2:234274/140‑1 (MQ=255)                          tGATTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGCGACTCATGGAAGCCGTCACATCCTGCAAGTACATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCa                                                                                                   <  2:197818/140‑1 (MQ=255)                          tGATTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGCGACTCATGGAAGCCGTCACATCCTGCAAGTACATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCa                                                                                                   <  1:257701/140‑1 (MQ=255)                            aTTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGCGACTCATGGAAGCCGTCACATCCTGCAAGTACATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCa                                                                                                   <  2:461508/138‑1 (MQ=255)                            aTTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGCGACTCATGGAAGCCGTCACATCCTGCAAGTACATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCa                                                                                                   >  1:461508/1‑138 (MQ=255)                            aTTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGCGACTCATGGAAGCCGTCACATCCTGCAAGTACATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACg                                                                                                 >  1:636276/1‑140 (MQ=255)                            aTTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGCGACTCATGGAAGCCGTCACATCCTGCAAGTACATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACg                                                                                                 >  2:317059/1‑140 (MQ=255)                            aTTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGCGACTCATGGAAGCCGTCACATCCTGCAAGTACATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACg                                                                                                 >  2:215455/1‑140 (MQ=255)                            aTTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGCGACTCATGGAAGCCGTCACATCCTGCAAGTACATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACg                                                                                                 >  1:430892/1‑140 (MQ=255)                                      aGCCTTTCAGCTGTTTCACAGGGCGACTCATGGAAGCCGTCACATCCTGCAAGTACATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTc                                                                                       <  1:1027815/140‑1 (MQ=255)                                      aGCCTTTCAGCTGTTTCACAGGGCGACTCATGGAAGCCGTCACATCCTGCAAGTACATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTc                                                                                       >  2:898599/1‑140 (MQ=255)                                       gCCTTTCAGCTGTTTCACAGGGCGACTCATGGAAGCCGTCACATCCTGCAAGTACATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCg                                                                                      <  2:430892/140‑1 (MQ=255)                                           ttCAGCTGTTTCACAGGGCGACTCATGGAAGCCGTCACATCCTGCAAGTACATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGgcgc                                                                                  >  1:1127625/1‑140 (MQ=255)                                              aGCTGTTTCACAGGGCGACTCATGGAAGCCGTCACATCCTGCAAGTACATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGa                                                                               <  2:1127625/140‑1 (MQ=255)                                               gCTGTTTCACAGGGCGACTCATGGAAGCCGTCACATCCTGCAAGTACATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGaa                                                                              <  2:591794/140‑1 (MQ=255)                                                  gTTTCACAGGGCGACTCATGGAAGCCGTCACATCCTGCAAGTACATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGAAAGt                                                                           >  2:14799/1‑140 (MQ=255)                                                    ttCACAGGGCGACTCATGGAAGCCGTCACATCCTGCAAGTACATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGAAAGttt                                                                         <  1:317059/140‑1 (MQ=255)                                                     tCACAGGGCGACTCATGGAAGCCGTCACATCCTGCAAGTACATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGAAAGTTTc                                                                        >  2:259746/1‑140 (MQ=255)                                                     tCACAGGGCGACTCATGGAAGCCGTCACATCCTGCAAGTACATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGAAAGTTTc                                                                        >  2:791322/1‑140 (MQ=255)                                                          gggCGACTCATGGAAGCCGTCACATCCTGCAAGTACATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGAAAGTTTCACATc                                                                   >  2:1170395/1‑140 (MQ=255)                                                             cGACTCATGGAAGCCGTCACATCCTGCAAGTACATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGAAAGtt                                                                          >  2:879656/1‑130 (MQ=255)                                                             cGACTCATGGAAGCCGTCACATCCTGCAAGTACATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGAAAGtt                                                                          <  1:879656/130‑1 (MQ=255)                                                             cGACTCATGGAAGCCGTCACATCCTGCAAGTACATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGAAAGTTTCACATCaga                                                                <  1:985459/140‑1 (MQ=255)                                                                cTCATGGAAGCCGTCACATCCTGCAAGTACATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGAAAGTTTCACATCAGAGAc                                                             <  1:1170395/140‑1 (MQ=255)                                                                   aTGGAAGCCGTCACATCCTGCAAGTACATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGAAAGTTTCACATCAGAGACGGt                                                          <  1:478538/140‑1 (MQ=255)                                                                        aGCCGTCACATCCTGCAAGTACATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGAAAGTTTCACATCAGAGACGGTGAAAg                                                     <  1:629815/140‑1 (MQ=255)                                                                        aGCCGTCACATCCTGCAAGTACATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGAAAGTTTCACATCAGAGACGGTGAAAg                                                     <  1:791322/140‑1 (MQ=255)                                                                        aGCCGTCACATCCTGCAAGTACATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGAAAGTTTCACATCAGAGACGGTGAAAg                                                     <  1:796517/140‑1 (MQ=255)                                                                           cGTCACATCCTGCAAGTACATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGAAAGTTTCACATCAGAGACGGTGAAAgtgg                                                  >  2:566615/1‑140 (MQ=255)                                                                             tCACATCCTGCAAGTACATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGAAAGTTTCACATCAGAGACgg                                                           <  2:197204/129‑1 (MQ=255)                                                                             tCACATCCTGCAAGTACATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGAAAGTTTCACATCAGAGACgg                                                           >  1:197204/1‑129 (MQ=255)                                                                             tCACATCCTGCAAGTACATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGAAAGTTTCACATCAGAGACGGTGAAAgtggtg                                                >  1:546692/1‑140 (MQ=255)                                                                                 aTCCTGCAAGTACATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGAAAGTTTCACATCAGAGACGGTGAAAGTGGTGTAGc                                            >  2:168535/1‑140 (MQ=255)                                                                                             cATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGAAAGTTTCACATCAGAGACGGTGAAAGTGGTGTAGCTCAGCCCATAGc                                >  2:146322/1‑140 (MQ=255)                                                                                             cATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGAAAGTTTCACATCAGAGACGGTGAAAGTGGTGTAGCTCAGCCCATAGc                                <  2:150258/140‑1 (MQ=255)                                                                                               tCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGAAAGTTTCACATCAGAGACGGTGAAAGTGGTGTAGCTCAGCCCATAGCCg                              <  2:613873/140‑1 (MQ=255)                                                                                                 tGCACTACCGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGAAAGTTTCACATCAGAGACGGTGAAAGTGGTGTAGCTCAGCCCATAGCCGaa                            <  1:146322/140‑1 (MQ=255)                                                                                                        ccGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGAAAGTTTCACATCAGAGACGGTGAAAGTGGTGTAGCTCAGCCCATAGCCGAACGGATAc                     >  2:899589/1‑140 (MQ=255)                                                                                                        ccGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGAAAGTTTCACATCAGAGACGGTGAAAGTGGTGTAGCTCAGCCCATAGCCGAACGGATAc                     >  2:784013/1‑140 (MQ=255)                                                                                                        ccGTGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGAAAGTTTCACATCAGAGACGGTGAAAGTGGTGTAGCTCAGCCCATAGCCGAACGGATAc                     >  1:42339/1‑140 (MQ=255)                                                                                                           tGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGAAAGTTTCACATCAGAGACGGTGAAAGTGGTGTAGCTCAGCCCATAGCCGAACGGATACAAc                  <  1:1045952/140‑1 (MQ=255)                                                                                                           tGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGAAAGTTTCACATCAGAGACGGTGAAAGTGGTGTAGCTCAGCCCATAGCCGAACGGATACAAc                  >  2:922382/1‑140 (MQ=255)                                                                                                           tGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGAAAGTTTCACATCAGAGACGGTGAAAGTGGTGTAGCTCAGCCCATAGCCGAACGGATACAAc                  >  2:811351/1‑140 (MQ=255)                                                                                                           tGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGAAAGTTTCACATCAGAGACGGTGAAAGTGGTGTAGCTCAGCCCATAGCCGAACGGATACAAc                  >  2:208115/1‑140 (MQ=255)                                                                                                           tGGCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGAAAGTTTCACATCAGAGACGGTGAAAGTGGTGTAGCTCAGCCCATAGCCGAACGGATACAAc                  <  1:259746/140‑1 (MQ=255)                                                                                                            ggCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGAAAGTTTCACATCAGAGACGGTGAAAGTGGTGTAGCTCAGCCCATAGCCGAACGGATACAACg                 <  1:14799/140‑1 (MQ=255)                                                                                                             gCACCCTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGAAAGTTTCACATCAGAGACGGTGAAAGTGGTGTAGCTCAGCCCATAGCCGAACGGATACAACGc                >  2:149137/1‑140 (MQ=255)                                                                                                                  cTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGAAAGTTTCACATCAGAGACGGTGAAAGTGGTGTAGCTCAGCCCATAGCCGAACGGATACAACGc                >  2:609887/1‑135 (MQ=255)                                                                                                                  cTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGAAAGTTTCACATCAGAGACGGTGAAAGTGGTGTAGCTCAGCCCATAGCCGAACGGATACAACGc                <  1:609887/135‑1 (MQ=255)                                                                                                                  cTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGAAAGTTTCACATCAGAGACGGTGAAAGTGGTGTAGCTCAGCCCATAGCCGAACGGATACAACGCCCCGt           >  2:726811/1‑140 (MQ=255)                                                                                                                  cTCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGAAAGTTTCACATCAGAGACGGTGAAAGTGGTGTAGCTCAGCCCATAGCCGAACGGATACAACGCCCCGt           >  1:496164/1‑140 (MQ=255)                                                                                                                   tCGCGCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGAAAGTTTCACATCAGAGACGGTGAAAGTGGTGTAGCTCAGCCCATAGCCGAACGGATACAACGCCCCGtt          >  2:995533/1‑140 (MQ=255)                                                                                                                    cgcgCTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTTGGCGCAGAAAGTTTCACATCAGAGACGGTGAAAGTGGTGTAGCTCAGCCCATAGCCGAACGGATACAACGCCCCGTTa         >  2:647633/1‑140 (MQ=255)                                                                                                                      cgcTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGAAAGTTTCACATCAGAGACGGTGAAAGTGGTGTAGCTCAGCCCATAGCCGAACGGATACAACGCCCCGTTAGc       <  1:898599/140‑1 (MQ=255)                                                                                                                      cgcTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGAAAGTTTCACATCAGAGACGGTGAAAGTGGTGTAGCTCAGCCCATAGCCGAACGGATACAACGCCCCGTTAGc       <  1:841701/140‑1 (MQ=255)                                                                                                                      cgcTTACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGAAAGTTTCACATCAGAGACGGTGAAAGTGGTGTAGCTCAGCCCATAGCCGAACGGATACAACGCCCCGTTAGc       <  1:605909/140‑1 (MQ=255)                                                                                                                         ttACCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGAAAGTTTCACATCAGAGACGGTGAAAGTGGTGTAGCTCAGCCCATAGCCGAACGGATACAACGCCCCGTTAGCTtc    <  1:1130222/140‑1 (MQ=255)                                                                                                                           aCCGGTGCTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGAAAGTTTCACATCAGAGACGGTGAAAGTGGTGTAGCTCAGCCCATAGCCGAACGGATACAACGCCCCGTTAGCTtcat  <  1:208115/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                     GTCTGAGTTTCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGCGACTCATGGAAGCCGTCACATCCTGCAAGTACATCTGCACTACCGTGGCACCCTCGCGCTTACCGGTGTTCGTCACCTGCACGCTGGCAGTCACTTTGCCGTCACGCTTCATGGTCGGCGCAGAAAGTTTCACATCAGAGACGGTGAAAGTGGTGTAGCTCAGCCCATAGCCGAACGGATACAACGCCCCGTTAGCTTCAT  >  NC_000913/2219831‑2220092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑