Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,338,063	T→C	Q453R (CAG→CGG)	gyrA ←	DNA gyrase subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,338,063	0	T	C	100.0%	191.6 / NA	54	Q453R (CAG→CGG)	gyrA	DNA gyrase subunit A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/28);  total (26/28)

TCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAGCAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCTGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGT  >  NC_000913/2337926‑2338199                                                                                                                                          |                                                                                                                                         tCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAGCAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCCGt                                                                                                                                        >  1:913281/1‑140 (MQ=255) tCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAGCAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCCGt                                                                                                                                        >  1:365375/1‑140 (MQ=255)  cAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAGCAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCCGtt                                                                                                                                       <  1:48609/140‑1 (MQ=255)  cAGACAATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAGCAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCCGtt                                                                                                                                       <  1:888657/140‑1 (MQ=255)     aCGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAGCAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCCGTTCgg                                                                                                                                    <  2:258850/140‑1 (MQ=255)     aCGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAGCAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCCGTTCgg                                                                                                                                    <  1:544923/140‑1 (MQ=255)     aCGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAGCAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCCGTTCgg                                                                                                                                    <  2:386229/140‑1 (MQ=255)     aCGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAGCAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCCGTTCgg                                                                                                                                    <  2:1020263/140‑1 (MQ=255)     aCGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAGCAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCCGTTCgg                                                                                                                                    <  1:162622/140‑1 (MQ=255)          cGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAGCAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCCGTTCGGTCggg                                                                                                                               <  2:159332/140‑1 (MQ=255)          cGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAGCAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCCGTTCGGTCAgg                                                                                                                               <  1:601510/140‑1 (MQ=255)          cGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAGCAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCCGTTCGGTCAgg                                                                                                                               <  1:203437/140‑1 (MQ=255)          cGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAGCAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCCGTTCGGTCAgg                                                                                                                               <  2:1003209/140‑1 (MQ=255)                   cAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAGCAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCCGTTCGGTCAGGTAGTACAGa                                                                                                                      <  1:576412/140‑1 (MQ=255)                       aaTACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAGCAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCCGTTCGGTCAGGTAGTACAGACCAt                                                                                                                  >  2:345965/1‑140 (MQ=255)                          aCGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAGCAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCCGTTCGGTCAGGTAGTACAGACCATcac                                                                                                               >  1:789904/1‑140 (MQ=255)                            gCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAGCAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCCGTTCGGTCAGGTAGTACAGACCATcacgc                                                                                                             >  1:1080188/1‑140 (MQ=255)                             cAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAGCAGTTTTTCGTGCTCAAGACCAGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCCGTTCGGTCAGGTAGTACAGACCATcacgca                                                                                                            >  1:170853/1‑140 (MQ=255)                                   agttccgATCTGATCCAGCAGCTCTTTGTATTCGTCGAGCAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCCGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGa                                                                                                      >  1:27605/6‑140 (MQ=255)                                       gcgATCTGATCCAGCAGCTCTTTGTATTCGTCGAGCAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCCGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAActc                                                                                                  <  2:789904/140‑1 (MQ=255)                                               aTCCAGCAGCTCTTTGTATTCGTCGAGCAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCCGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCa                                                                                          <  1:345965/140‑1 (MQ=255)                                               aTCCAGCAGCTCTTTGTATTCGTCGAGCAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCCGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCa                                                                                          <  2:1085870/140‑1 (MQ=255)                                                tCCAGCAGCTCTTTGTATTCGTCGAGCAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCCGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAg                                                                                         >  1:706472/1‑140 (MQ=255)                                                     cagcTCTTTGTATTCGTCGAGCAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCCGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCAtt                                                                                    >  2:353294/1‑140 (MQ=255)                                                         tctTTGTATTCGTCGAGCAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCCGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCgg                                                                                >  2:808340/1‑140 (MQ=255)                                                                aTTCGTCGAGCAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCCGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCgca                                                                         <  2:456776/140‑1 (MQ=255)                                                                    gtcgAGCAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCCGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCAt                                                                                     >  2:999244/1‑124 (MQ=255)                                                                    gtcgAGCAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCCGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCAt                                                                                     <  1:999244/124‑1 (MQ=255)                                                                     tcgAGCAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCCGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCAtc                                                                    >  1:514636/1‑140 (MQ=255)                                                                       gAGCAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCCGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCAtcgt                                                                  >  2:239429/1‑140 (MQ=255)                                                                       gAGCAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCCGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCAtcgt                                                                  >  2:158124/1‑140 (MQ=255)                                                                         gCAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCCGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCAtcgtcg                                                                >  1:531071/1‑140 (MQ=255)                                                                               tttCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCCGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCa                                                          <  2:489919/140‑1 (MQ=255)                                                                                      cTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCCGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGa                                                   >  1:362456/1‑140 (MQ=255)                                                                                               ggTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCCGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCgg                                          <  2:1000768/140‑1 (MQ=255)                                                                                                gTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCCGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCa                                                          >  2:24765/1‑123 (MQ=255)                                                                                                gTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCCGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCa                                                          <  1:24765/123‑1 (MQ=255)                                                                                                     tttCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCCGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGt                                    >  2:1169519/1‑140 (MQ=255)                                                                                                     tttCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCCGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGt                                    >  1:1087957/1‑140 (MQ=255)                                                                                                       tCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCCGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTg                                  <  1:353294/140‑1 (MQ=255)                                                                                                           cAAACGCAGATCCAGAATCGCCTGAGCTTGCCGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCa                              >  1:100776/1‑140 (MQ=255)                                                                                                               cGCAGATCCAGAATCGCCTGAGCTTGCCGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTg                          >  2:942286/1‑140 (MQ=255)                                                                                                                gCAGATCCAGAATCGCCTGAGCTTGCCGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGc                         <  2:365375/140‑1 (MQ=255)                                                                                                                  aGATCCAGAATCGCCTGAGCTTGCCGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCa                       <  2:514636/140‑1 (MQ=255)                                                                                                                   gATCCAGAATCGCCTGAGCTTGCCGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCAc                      >  1:746690/1‑140 (MQ=255)                                                                                                                   gATCCAGAATCGCCTGAGCTTGCCGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCAc                      <  1:75141/140‑1 (MQ=255)                                                                                                                         gAATCGCCTGAGCTTGCCGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTa                <  2:913281/140‑1 (MQ=255)                                                                                                                          aaTCGCCTGAGCTTGCCGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTAg               >  1:711186/1‑140 (MQ=255)                                                                                                                               ccTGAGCTTGCCGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTAGCAAcc          >  1:985815/1‑140 (MQ=255)                                                                                                                               ccTGAGCTTGCCGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTAGCAAcc          <  2:170853/140‑1 (MQ=255)                                                                                                                                cTGAGCTTGCCGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTAGCAACCa         >  1:815003/1‑140 (MQ=255)                                                                                                                                   aGCTTGCCGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTAGCAACCAgcg      <  2:100776/140‑1 (MQ=255)                                                                                                                                     cTTGCCGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCa    >  1:556361/1‑140 (MQ=255)                                                                                                                                       tGCCGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGt  <  2:531071/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         TCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAGCAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCTGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGT  >  NC_000913/2337926‑2338199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑