Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,354,450	T→C	S62S (AGT→AGC)	glpB →	anaerobic glycerol‑3‑phosphate dehydrogenase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,354,450	0	T	C	100.0%	121.3 / NA	35	S62S (AGT→AGC)	glpB	anaerobic glycerol‑3‑phosphate dehydrogenase subunit B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/22);  total (13/22)

TGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGA  >  NC_000913/2354315‑2354584                                                                                                                                        |                                                                                                                                       tgGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCACTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGCGGAc                                                                                                                                    >  1:142150/1‑140 (MQ=255)      tgcaactgcaaAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGCGGACTGGaa                                                                                                                               >  2:516528/1‑140 (MQ=255)          actgcaaAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGGTCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGCGGACTGGAATCtt                                                                                                                           <  1:773009/140‑1 (MQ=255)           ctgcaaAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGCGGACTGGAATCttt                                                                                                                          >  2:113854/1‑140 (MQ=255)               aaaaaCACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGCGGACTGGAATCTTTGCGt                                                                                                                      <  2:1026049/140‑1 (MQ=255)                 aaaCACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGCGGACTGGAATCTTTGCGTca                                                                                                                    <  1:20095/140‑1 (MQ=255)                              gcTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGCGGACTGGAATCTTTGCGTCAGCAGGCACCAGcc                                                                                                       <  1:464177/140‑1 (MQ=255)                               cTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGCGGACTGGAATCTTTGCGTCAGCAGGCACCAGccc                                                                                                      <  2:875599/140‑1 (MQ=255)                               cTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGCGGACTGGAATCTTTGCGTCAGCAGGCACCAGccc                                                                                                      <  1:516528/140‑1 (MQ=255)                                tgtgCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGCGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCa                                                                                                     <  1:113854/140‑1 (MQ=255)                                tgtgCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGCGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCa                                                                                                     <  2:225074/140‑1 (MQ=255)                                 gtgCCATTGTTACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGCGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCAt                                                                                                    <  2:591949/140‑1 (MQ=255)                                    ccATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGCGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCt                                                                                                 <  2:148041/140‑1 (MQ=255)                                     cATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGCGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCtt                                                                                                <  1:987549/140‑1 (MQ=255)                                     cATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGCGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCtt                                                                                                <  1:894951/140‑1 (MQ=255)                                              tCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGCGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACt                                                                                             <  1:200164/134‑1 (MQ=255)                                              tCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGCGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACt                                                                                             >  2:200164/1‑134 (MQ=255)                                                 tGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGCGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCg                                                                                    >  1:558325/1‑140 (MQ=255)                                                      aaaGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGCGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCca                                                                               <  2:296108/140‑1 (MQ=255)                                                          cgcACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGCGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAAc                                                                           <  2:662689/140‑1 (MQ=255)                                                          cgcACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACACCGGCCTGGAATCTTAGCGTCAGCAGGCCCAAGCCCATCCTTCCTCCCTGCCCGAGCCACAAc                                                                           <  2:58651/140‑1 (MQ=255)                                                             aCTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGCGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAAcgcg                                                                        >  2:419827/1‑140 (MQ=255)                                                                         aTCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGCGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCg                                                            >  1:782970/1‑140 (MQ=255)                                                                           ccGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGCGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAAc                                                                           <  1:259623/123‑1 (MQ=255)                                                                           ccGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGCGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAAc                                                                           >  2:259623/1‑123 (MQ=255)                                                                              gATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGCGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGc                                                       >  1:1110011/1‑140 (MQ=255)                                                                                   cTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGCGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGgc                                                  >  1:65925/1‑140 (MQ=255)                                                                                    tGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGCGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGgcg                                                 <  1:340412/140‑1 (MQ=255)                                                                                                     cTGCCAGATGGTCAACCGGTGACAGACATTCACAGCGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGa                                >  1:803609/1‑140 (MQ=255)                                                                                                          aGATGGTCAACCGGTGACAGACATTCACAGCGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCg                           <  1:798367/140‑1 (MQ=255)                                                                                                             tGGTCAACCGGTGACAGACATTCACAGCGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTg                        <  2:1110011/140‑1 (MQ=255)                                                                                                               gTCAACCGGTGACAGACATTCACAGCGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTgcg                      >  1:996242/1‑140 (MQ=255)                                                                                                                tCAACCGGTGACAGACATTCACAGCGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCTAGGCGCAGGCATTAATCGCTGAAAGCGGTgcgc                     <  2:142150/140‑1 (MQ=255)                                                                                                                tCAACCGGTGACAGACATTCACAGCGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTgcgc                     <  1:419827/140‑1 (MQ=255)                                                                                                                tCAACCGGTGACAGACATTCACAGCGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTgcgc                     >  1:1083080/1‑140 (MQ=255)                                                                                                                                   cacaGCGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGa  <  2:1083080/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       TGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGA  >  NC_000913/2354315‑2354584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑