Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,375,148	G→A	T272M (ACG→ATG)	menC ←	o‑succinylbenzoate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,375,148	0	G	A	100.0%	175.1 / NA	51	T272M (ACG→ATG)	menC	o‑succinylbenzoate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/25);  total (26/25)

GCTCCAGTGCATCAACTTCCACGACAGGCAGCGTGCTACCCGGCCAGCGACGTACCTGCTGCGCCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAA‑CCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACG  >  NC_000913/2375011‑2375279                                                                                                                                           |                                                                                                                                    gCTCCAGTGCATCAACTTCCACGACAGGCAGCGTGCTACCCGGCCAGCGACGTACCTGCTGCGCCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAA‑CCAGGCGGCAATCCGCGCCAGTTGCAtt                                                                                                                                   >  2:800919/1‑140 (MQ=255)  cTCCAGTGCATCAACTTCCACGACAGGCAGCGTGCTACCCGGCCAGCGACGTACCTGCTGCGCCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAA‑CCAGGCGGCAATCCGCGCCAGTTGCATTa                                                                                                                                  >  2:586790/1‑140 (MQ=255)       gTGCATCAACTTCCACGACAGGCAGCGTGCTACCCGGCCAGCGACGTACCTGCTGCGCCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAA‑CCAGGCGGCAATCCGCGCCAGTTGCATTAAGCCt                                                                                                                             >  1:1051839/1‑140 (MQ=255)          cATCAACTTCCACGACAGGCAGCGTGCTACCCGGCCAGCGACGTACCTGCTGCCCCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAA‑CCAGGCGGCAATCCGCGCCAGTTGCATTAAGCCTAAg                                                                                                                          >  2:945263/1‑140 (MQ=255)           aTCAACTTCCACGACAGGCAGCGTGCTACCCGGCCAGCGACGTACCTGCTGCGCCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAACCCAGGCGGCAATCCGCGCCAGTTGCATTAAGCCTAAg                                                                                                                          <  2:402247/140‑1 (MQ=255)               aCTTCCACGACAGGCAGCGTGCTACCCGGCCAGCGACGTACCTGCTGCGCCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAA‑CCAGGCGGCAATCCGCGCCAGTTGCATTAAGCCTAAGCTCGa                                                                                                                     <  2:1051839/140‑1 (MQ=255)                  tCCACGACAGGCAGCGTGCTACCCGGCCAGCGACGTACCTGCTGCGCCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAA‑CCAGGCGGCAATCCGCGCCAGTTGCATTAAGCCTAAGCTCGATTc                                                                                                                  <  2:169221/140‑1 (MQ=255)                    cACGACAGGCAGCGTGCTACCCGGCCAGCGACGTACCTGCTGCGCCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAA‑CCAGGCGGCAATCCGCGCCAGTTGCATTAAGCCTAAGCTCGATTCaa                                                                                                                >  1:601336/1‑140 (MQ=255)                         cAGGCAGCGTGCTACCCGGCCAGCGACGTACCTGCTGCGCCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAA‑CCAGGCGGCAATCCGCGCCAGTTGCATTAAGCCTAAGCTCGATTCAATGgaa                                                                                                           >  1:956707/1‑140 (MQ=255)                          aGGCAGCGTGCTACCCGGCCAGCGACGTACCTGCTGCGCCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAA‑CCAGGCGGCAATCCGCGCCAGTTGCATTAAGCCTAAGCTCGATTCAATGgaag                                                                                                          <  1:1380/140‑1 (MQ=255)                          aGGCAGCGTGCTACCCGGCCAGCGACGTACCTGCTGCGCCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAA‑CCAGGCGGCAATCCGCGCCAGTTGCATTAAGCCTAAGCTCGATTCAATGgaag                                                                                                          >  1:607496/1‑140 (MQ=255)                            gCAGCGTGCTACCCGGCCAGCGACGTACCTGCTGCGCCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAA‑CCAGGCGGCAATCCGCGCCAGTTGCATTAAGCCTAAGCTCGATTCAATGgaagaa                                                                                                        >  1:15142/1‑140 (MQ=255)                                          ggCCAGCGACGTACCTGCTGCCCCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAA‑CCAGGCGGCAATCCGCGCCAGTTGCATTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGt                                                                                          <  1:945263/140‑1 (MQ=255)                                               gCGACGTACCTGCTGCGCCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAA‑CCAGGCGGCAATCCGCGCCAGTTGCATTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGcc                                                                                     >  1:775307/1‑140 (MQ=255)                                                     tACCTGCTGCGCCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAA‑CCAGGCGGCAATCCGCGCCAGTTGCATTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAgcg                                                                               >  2:14076/1‑140 (MQ=255)                                                           ctgcGCCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAA‑CCAGGCGGCAATCCGCGCCAGTTGCATTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTgcg                                                                         <  2:601336/140‑1 (MQ=255)                                                           ctgcGCCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAA‑CCAGGCGGCAATCCGCGCCAGTTGCATTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTgcg                                                                         <  1:800919/140‑1 (MQ=255)                                                               gcCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAA‑CCAGGCGGCAATCCGCGCCAGTTGCATTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGcgccgc                                                                     <  2:775307/140‑1 (MQ=255)                                                               gcCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAA‑CCAGGCGGCAATCCGCGCCAGTTGCATTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGcgccgc                                                                     >  2:1030110/1‑140 (MQ=255)                                                                        aGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAA‑CCAGGCGGCAATCCGCGCCAGTTGCATTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTg                                                            <  1:1030110/140‑1 (MQ=255)                                                                            ccAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAA‑CCAGGCGGCAATCCGCGCCAGTTGCATTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTcg                                                        <  2:1097641/140‑1 (MQ=255)                                                                              aGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAA‑CCAGGCGGCAATCCGCGCCAGTTGCATTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCGGCCCCAGCGCGTGCGCCGCCTGTACCTGCTcgcg                                                      >  2:965526/1‑140 (MQ=255)                                                                              aGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAA‑CCAGGCGGCAATCCGCGCCAGTTGCATTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTcgcg                                                      >  2:91529/1‑140 (MQ=255)                                                                               gCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAA‑CCAGGCGGCAATCCGCGCCAGTTGCATTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGt                                                     >  1:1088320/1‑140 (MQ=255)                                                                                cGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAA‑CCAGGCGGCAATCCGCGCCAGTTGCATTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTa                                                    >  2:608716/1‑140 (MQ=255)                                                                                cGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAA‑CCAGGCGGCAATCCGCGCCAGTTGCATTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTa                                                    >  2:470190/1‑140 (MQ=255)                                                                                 gtgtCCAGCCCTGGAATGGTGTCCGGCGTTAA‑CCAGGCGGCAATCCGCGCCAGTTGCATTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTAc                                                   >  2:907161/1‑140 (MQ=255)                                                                                 gtgtCCAGCCCTGGAATGGTGTCCGGCGTTAA‑CCAGGCGGCAATCCGCGCCAGTTGCATTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTAc                                                   <  2:15142/140‑1 (MQ=255)                                                                                    tCCAGCCCTGGAATGGTGTCCGGCGTTAA‑CCAGGCGGCAATCCGCGCCAGTTGCATTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACttt                                                >  2:1105138/1‑140 (MQ=255)                                                                                      cAGCCCTGGAATGGTGTCCGGCGTTAA‑CCAGGCGGCAATCCGCGCCAGTTGCATTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACttttt                                              <  1:300775/140‑1 (MQ=255)                                                                                           cTGGAATGGTGTCCGGCGTTAA‑CCAGGCGGCAATCCGCGCCAGTTGCATTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGa                                         <  1:91529/140‑1 (MQ=255)                                                                                           cTGGAATGGTGTCCGGCGTTAA‑CCAGGCGGCAATCCGCGCCAGTTGCATTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGa                                         <  2:1088320/140‑1 (MQ=255)                                                                                           cTGGAATGGTGTCCGGCGTTAA‑CCAGGCGGCAATCCGCGCCAGTTGCATTAAGCCTAAGCTCGATCCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGa                                         <  2:873368/140‑1 (MQ=255)                                                                                                   gtgtCCGGCGTTAA‑CCAGGCGGCAATCCGCGCCAGTTGCATTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCa                                           >  2:144062/1‑130 (MQ=255)                                                                                                   gtgtCCGGCGTTAA‑CCAGGCGGCAATCCGCGCCAGTTGCATTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCa                                           <  1:144062/130‑1 (MQ=255)                                                                                                   gtgtCCGGCGTTAA‑CCAGGCGGCAATCCGCGCCAGTTGCATTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGt                                 >  1:33822/1‑140 (MQ=255)                                                                                                      tCCGGCGTTAA‑CCAGGCGGCAATCCGCGCCAGTTGCATTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAg                              >  1:912958/1‑140 (MQ=255)                                                                                                        cGGCGTTAA‑CCAGGCGGCAATCCGCGCCAGTTGCATTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCg                            <  1:365546/140‑1 (MQ=255)                                                                                                        cGGCGTTAA‑CCAGGCGGCAATCCGCGCCAGTTGCATTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCg                            >  2:1013566/1‑140 (MQ=255)                                                                                                        cGGCGTTAA‑CCAGGCGGCAATCCGCGCCAGTTGCATTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCg                            <  1:355170/140‑1 (MQ=255)                                                                                                          gCGTTAA‑CCAGGCGGCAATCCGCGCCAGTTGCATTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTg                          <  1:191297/140‑1 (MQ=255)                                                                                                             ttAA‑CCAGGCGGCAATCCGCGCCAGTTGCATTAAGCCTAAGCTCGATTCAATGGAAGAACTTATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGt                       <  2:607496/140‑1 (MQ=255)                                                                                                             ttAA‑CCAGGCGGCAATCCGCGCCAGTTGCATTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGt                       <  2:678154/140‑1 (MQ=255)                                                                                                             ttAA‑CCAGGCGGCAATCCGCGCCAGTTGCATTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGt                       <  2:1065481/140‑1 (MQ=255)                                                                                                             ttAA‑CCAGGCGGCAATCCGCGCCAGTTGCATTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCACCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGt                       >  2:759938/1‑140 (MQ=255)                                                                                                                a‑CCAGGCGGCAATCCGCGCCAGTTGCATTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTg                    >  2:230050/1‑140 (MQ=255)                                                                                                                      gcggcAATCCGCGCCAGTTGCATTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGt                       >  1:782508/1‑132 (MQ=255)                                                                                                                      gcggcAATCCGCGCCAGTTGCATTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGt                       <  2:782508/132‑1 (MQ=255)                                                                                                                        ggcAATCCGCGCCAGTTGCATTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTa             >  1:357496/1‑140 (MQ=255)                                                                                                                                gcgcCAGTTGCATTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCgcgcgc     <  1:586790/140‑1 (MQ=255)                                                                                                                                   ccAGTTGCATTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACg  <  2:956707/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                    GCTCCAGTGCATCAACTTCCACGACAGGCAGCGTGCTACCCGGCCAGCGACGTACCTGCTGCGCCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAA‑CCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACG  >  NC_000913/2375011‑2375279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑