Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,432,239	T→C	D235G (GAC→GGC)	folC ←	bifunctional folylpolyglutamate synthetase/dihydrofolate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,432,239	0	T	C	100.0%	270.9 / NA	76	D235G (GAC→GGC)	folC	bifunctional folylpolyglutamate synthetase/dihydrofolate synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/47);  total (29/47)

TCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGTCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCGGCTCACCGACAATTGCCGGTTTTTCGCTGCGGAAGATGC  >  NC_000913/2432107‑2432374                                                                                                                                     |                                                                                                                                        tCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATc                                                                                                                                  <  1:503227/140‑1 (MQ=255)  cgcgAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTGTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATAATCg                                                                                                                                 >  2:1075109/1‑140 (MQ=255)    cgAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGt                                                                                                                               <  2:852552/140‑1 (MQ=255)      aaTGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGa                                                                                                                             <  2:355542/140‑1 (MQ=255)      aaTGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGa                                                                                                                             <  1:798573/140‑1 (MQ=255)      aaTGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGa                                                                                                                             <  2:362471/140‑1 (MQ=255)      aaTGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGa                                                                                                                             <  1:166969/140‑1 (MQ=255)      aaTGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGa                                                                                                                             >  2:84008/1‑140 (MQ=255)      aaTGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGa                                                                                                                             <  2:1034044/140‑1 (MQ=255)       aTGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGAc                                                                                                                            <  1:708517/140‑1 (MQ=255)        tGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACg                                                                                                                           >  2:114886/1‑140 (MQ=255)           cATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGaa                                                                                                                        >  1:781918/1‑140 (MQ=255)           cATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGaa                                                                                                                        <  1:146302/140‑1 (MQ=255)           cATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGaa                                                                                                                        <  1:1145357/140‑1 (MQ=255)           cATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGaa                                                                                                                        <  2:910234/140‑1 (MQ=255)                     gACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACt                                                                                                              >  2:671214/1‑140 (MQ=255)                       cTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCa                                                                                                            >  1:161870/1‑140 (MQ=255)                              cccGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGcccc                                                                                                     <  1:884568/140‑1 (MQ=255)                              cccGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGcccc                                                                                                     <  1:671214/140‑1 (MQ=255)                              cccGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGcccc                                                                                                     <  1:605834/140‑1 (MQ=255)                              cccGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGcccc                                                                                                     <  1:814428/140‑1 (MQ=255)                              cccGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGcccc                                                                                                     <  2:1134516/140‑1 (MQ=255)                              cccGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGcccc                                                                                                     <  2:211079/140‑1 (MQ=255)                              cccGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGcccc                                                                                                     <  2:376985/140‑1 (MQ=255)                              cccGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGcccc                                                                                                     >  1:351814/1‑140 (MQ=255)                              cccGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGcccc                                                                                                     <  2:78906/140‑1 (MQ=255)                              cccGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGcccc                                                                                                     <  2:828133/140‑1 (MQ=255)                              cccGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGcccc                                                                                                     <  1:114886/140‑1 (MQ=255)                              cccGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGcccc                                                                                                     <  2:985201/140‑1 (MQ=255)                                cGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGa                                                                                                   <  2:134248/140‑1 (MQ=255)                                   tGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGt                                                                                                <  1:1075109/140‑1 (MQ=255)                                             tGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTg                                                                                      >  1:1130484/1‑140 (MQ=255)                                               ccgccAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGa                                                                                                   >  2:445825/1‑125 (MQ=255)                                               ccgccAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGa                                                                                                   <  1:445825/125‑1 (MQ=255)                                               ccgccAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTaa                                                                                           <  1:836601/133‑1 (MQ=255)                                               ccgccAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTaa                                                                                           >  2:836601/1‑133 (MQ=255)                                                   cAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCtt                                                                                >  2:859840/1‑140 (MQ=255)                                                       gcTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTc                                                                            >  2:212633/1‑140 (MQ=255)                                                       gcTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTc                                                                            <  1:84008/140‑1 (MQ=255)                                                       gcTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTc                                                                            >  2:151786/1‑140 (MQ=255)                                                        cTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCTAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTcc                                                                           <  2:906074/140‑1 (MQ=255)                                                        cTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTcc                                                                           <  2:351814/140‑1 (MQ=255)                                                             gCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTgcgc                                                                      <  1:151786/140‑1 (MQ=255)                                                                gCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCcac                                                                   >  2:367281/1‑140 (MQ=255)                                                                gCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCcac                                                                   <  1:212633/140‑1 (MQ=255)                                                                gCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCcac                                                                   <  2:124748/140‑1 (MQ=255)                                                                 cATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCcaca                                                                  <  1:92018/140‑1 (MQ=255)                                                                   ttCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATc                                                                >  2:1102132/1‑140 (MQ=255)                                                                   ttCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATc                                                                >  1:868572/1‑140 (MQ=255)                                                                     cGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAg                                                              <  2:952686/140‑1 (MQ=255)                                                                     cGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAg                                                              >  1:34417/1‑140 (MQ=255)                                                                     cGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAg                                                              <  1:859840/140‑1 (MQ=255)                                                                     cGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAg                                                              >  1:360496/1‑140 (MQ=255)                                                                           cGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAGCAATgg                                                        >  1:242216/1‑140 (MQ=255)                                                                              gACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAGCAATGGTAg                                                     <  2:781918/140‑1 (MQ=255)                                                                                cAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAGCAATGGTAGaa                                                   >  1:1039617/1‑140 (MQ=255)                                                                                 aaGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAGCAATGGTAGAAg                                                  <  2:360496/140‑1 (MQ=255)                                                                                        aaCGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAGCAATGGTAGAAGGCATTTc                                           <  1:414997/140‑1 (MQ=255)                                                                                          cGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCg                                         >  2:119836/1‑140 (MQ=255)                                                                                                 ttttCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCGGCTCAcc                                  >  2:452352/1‑140 (MQ=255)                                                                                                 ttttCCAGCGTGCCGTAAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCGGCTCAcc                                  >  2:547528/1‑140 (MQ=255)                                                                                                    tCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCGGCTCACCGAc                               >  2:675715/1‑140 (MQ=255)                                                                                                     ccAGCGTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCGGCTCACCGACa                              <  1:452352/140‑1 (MQ=255)                                                                                                     ccAGCGTGCCGTAAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCGGCTCACCGACa                              <  1:547528/140‑1 (MQ=255)                                                                                                          gTGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCGGCTCACCGACAATTGc                         >  1:567193/1‑140 (MQ=255)                                                                                                           tGCCGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCGGCTCACCGACAATTGcc                        >  2:322824/1‑140 (MQ=255)                                                                                                             ccGTGAGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCGGCTCACCGACAATTGCCgg                      <  1:367281/140‑1 (MQ=255)                                                                                                                  aGCATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCGGCTCACCGACAATTGCCGGttttt                 >  2:131331/1‑140 (MQ=255)                                                                                                                    cATCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCGGCTCACCGACAATTGCCGGTTTTTCg               <  2:34417/140‑1 (MQ=255)                                                                                                                     aTCGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCGGCTCACCGACAATTGCCGGTTTTTCGc              >  2:276587/1‑140 (MQ=255)                                                                                                                       cGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCGGCTCACCGACAATTGCCGGttttt                 >  1:70512/1‑135 (MQ=255)                                                                                                                       cGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCGGCTCACCGACAATTGCCGGttttt                 <  2:70512/135‑1 (MQ=255)                                                                                                                       cGCTAAACGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCGGCTCACCGACAATTGCCGGTTTTTCGCTg            <  2:161870/140‑1 (MQ=255)                                                                                                                           aaaCGCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCGGCTCACCGACAATTGCCGGTTTTTCGCTGCGGa        <  1:758915/140‑1 (MQ=255)                                                                                                                               gCCCAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCGGCTCACCGACAATTGCCGGTTTTTCGCTGCGGAAGAt    <  1:322824/140‑1 (MQ=255)                                                                                                                                 ccAGCCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCGGCTCACCGACAATTGCCGGTTTTTCGCTGCGGAAGATGc  <  2:567193/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                        TCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGTCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCGGCTCACCGACAATTGCCGGTTTTTCGCTGCGGAAGATGC  >  NC_000913/2432107‑2432374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑