Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,504,447	A→G	G13G (GGT→GGC)	fryA ←	putative PTS multiphosphoryl transfer protein FryA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,504,447	0	A	G	100.0%	269.1 / NA	75	G13G (GGT→GGC)	fryA	putative PTS multiphosphoryl transfer protein FryA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/36);  total (39/36)

GGGTGCCGGTGCCAATCAGCGCCA‑‑GCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCT  >  NC_000913/2504314‑2504580                                                                                                                                        |                                                                                                                                      gggTGCCGGTGCCAATCAGCGCCA‑‑GCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTa                                                                                                                                 >  1:1167251/1‑140 (MQ=255) gggTGCCGGTGCCAATCAGCGCCA‑‑GCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTa                                                                                                                                 >  1:944801/1‑140 (MQ=255) gggTGCCGGTGCCAATCAGCGCCA‑‑GCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTa                                                                                                                                 >  2:196403/1‑140 (MQ=255) gggTGCCGGTGCCAATCAGCGCCA‑‑GCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTa                                                                                                                                 >  2:374812/1‑140 (MQ=255)  ggtgccggtgccAATCAGCGCCA‑‑GCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAg                                                                                                                                >  2:1026435/1‑140 (MQ=255)  ggtgccggtgccAATCAGCGCCA‑‑GCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAg                                                                                                                                >  2:893087/1‑140 (MQ=255)  ggtgccggtgccAATCAGCGCCA‑‑GCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAg                                                                                                                                >  2:874552/1‑140 (MQ=255)  ggtgccggtgccAATCAGCGCCA‑‑GCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAg                                                                                                                                >  1:72561/1‑140 (MQ=255)  ggtgccggtgccAATCAGCGCCA‑‑GCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAg                                                                                                                                >  2:652144/1‑140 (MQ=255)    tgccggtgccAATCAGCGCCA‑‑GCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGtt                                                                                                                                  <  2:410931/136‑1 (MQ=255)    tgccggtgccAATCAGCGCCA‑‑GCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGtt                                                                                                                                  >  1:410931/1‑136 (MQ=255)    tgccggtgccAATCAGCGCCA‑‑GCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGc                                                                                                                              >  2:49068/1‑140 (MQ=255)    tgccggtgccAATCAGCGCCA‑‑GCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGc                                                                                                                              >  2:1010183/1‑140 (MQ=255)     gccggtgccAATCAGCGCCA‑‑GCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCa                                                                                                                             <  1:452744/140‑1 (MQ=255)     gccggtgccAATCAGCGCCA‑‑GCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCa                                                                                                                             >  1:225329/1‑140 (MQ=255)      ccggtgccAATCAGCGCCA‑‑GCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCaa                                                                                                                            <  1:180629/140‑1 (MQ=255)      ccggtgccAATCAGCGCCA‑‑GCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCaa                                                                                                                            >  1:687052/1‑140 (MQ=255)       cggtgccAATCAGCGCCA‑‑GCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCaaa                                                                                                                           >  2:675492/1‑140 (MQ=255)       cggtgccAATCAGCGCCA‑‑GCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCaaa                                                                                                                           >  2:828634/1‑140 (MQ=255)       cggtgccAATCAGCGCCA‑‑GCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCaaa                                                                                                                           >  1:547725/1‑140 (MQ=255)          tgccAATCAGCGCCA‑‑GCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGa                                                                                                                        <  2:103745/140‑1 (MQ=255)             cAATCAGCGCCAGCGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGAc                                                                                                                       >  2:11748/1‑140 (MQ=255)                    cgcCA‑‑GCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAAtt                                                                                                              <  1:196403/140‑1 (MQ=255)                      ccA‑‑GCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGa                                                                                                            >  2:773215/1‑140 (MQ=255)                      ccA‑‑GCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGa                                                                                                            <  2:225329/140‑1 (MQ=255)                        a‑‑GCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAAt                                                                                                          >  2:372813/1‑140 (MQ=255)                                    tCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATa                                                                                                <  2:1167251/140‑1 (MQ=255)                                         tCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGc                                                                                           >  1:962599/1‑140 (MQ=255)                                         tCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGc                                                                                           >  1:659127/1‑140 (MQ=255)                                          cTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCt                                                                                          <  1:374812/140‑1 (MQ=255)                                          cTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCt                                                                                          <  1:828634/140‑1 (MQ=255)                                          cTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCt                                                                                          <  1:652144/140‑1 (MQ=255)                                           tGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTc                                                                                         <  2:591789/140‑1 (MQ=255)                                           tGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTc                                                                                         >  1:561868/1‑140 (MQ=255)                                                ttGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGt                                                                                    <  1:49068/140‑1 (MQ=255)                                                ttGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGt                                                                                    <  1:11748/140‑1 (MQ=255)                                                ttGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGt                                                                                    <  1:1010183/140‑1 (MQ=255)                                                          gCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGgag                                                                          <  1:675492/140‑1 (MQ=255)                                                          gCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGgag                                                                          <  2:72561/140‑1 (MQ=255)                                                            gATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGat                                                                        <  1:774462/140‑1 (MQ=255)                                                                        aaGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATcc                                                            <  1:102050/140‑1 (MQ=255)                                                                          gTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGt                                                          <  1:1026435/140‑1 (MQ=255)                                                                          gTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGt                                                          <  1:893087/140‑1 (MQ=255)                                                                                  gCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGaa                                                  <  1:874552/140‑1 (MQ=255)                                                                                     ttGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGaacaa                                               >  2:288475/1‑140 (MQ=255)                                                                                     ttGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTCAAATCCGTCAGTTGaacaa                                               >  2:1141553/1‑140 (MQ=255)                                                                                      tGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGaacaac                                              <  1:773215/140‑1 (MQ=255)                                                                                           cTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGtct                                         <  1:479758/140‑1 (MQ=255)                                                                                                tGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCAcgc                                    >  2:967257/1‑140 (MQ=255)                                                                                                tGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCAcgc                                    >  2:929111/1‑140 (MQ=255)                                                                                                   aCTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTa                                 <  1:288475/140‑1 (MQ=255)                                                                                                   aCTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTa                                 <  2:687052/140‑1 (MQ=255)                                                                                                   aCTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTa                                 <  2:561868/140‑1 (MQ=255)                                                                                                   aCTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTCAAATCCGTCAGTTGAACAACCGTCTCACGCGTa                                 <  1:1141553/140‑1 (MQ=255)                                                                                                       ttCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGAc                             >  1:655115/1‑140 (MQ=255)                                                                                                        tCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAGCAACCGTCTCACGCGTAAGACg                            >  1:85986/1‑140 (MQ=255)                                                                                                        tCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACg                            >  2:1124701/1‑140 (MQ=255)                                                                                                        tCTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACg                            >  1:1047732/1‑140 (MQ=255)                                                                                                         cTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGt                           <  2:944801/140‑1 (MQ=255)                                                                                                         cTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGt                           <  1:372813/140‑1 (MQ=255)                                                                                                         cTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGt                           <  2:1047732/140‑1 (MQ=255)                                                                                                         cTTTAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGt                           <  2:547725/140‑1 (MQ=255)                                                                                                          tttAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGtctc                                        >  2:639378/1‑126 (MQ=255)                                                                                                          tttAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGtctc                                        <  1:639378/126‑1 (MQ=255)                                                                                                            tAAGTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGa                        >  2:373620/1‑140 (MQ=255)                                                                                                               gTTTCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTa                                 >  1:380172/1‑128 (MQ=255)                                                                                                               gTTCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTa                                 <  2:380172/128‑1 (MQ=255)                                                                                                                 tCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATaa                    >  2:882048/1‑139 (MQ=255)                                                                                                                 tCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATaa                    <  1:882048/139‑1 (MQ=255)                                                                                                                 tCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAg                   >  1:548629/1‑140 (MQ=255)                                                                                                                 tCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAg                   >  2:487411/1‑140 (MQ=255)                                                                                                                 tCCCACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAg                   >  2:189841/1‑140 (MQ=255)                                                                                                                   ccACGCCGGACGAGCATGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAggg                 >  1:355920/1‑140 (MQ=255)                                                                                                                                  aTGTAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTtgct  <  1:929111/140‑1 (MQ=255)                                                                                                                                     tAGGCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATaaa         <  2:517140/130‑1 (MQ=255)                                                                                                                                        |                                                                                                                                      GGGTGCCGGTGCCAATCAGCGCCA‑‑GCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCT  >  NC_000913/2504314‑2504580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑