Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,680,542	G→A	A735V (GCG→GTG)	yphG ←	DUF5107 domain‑containing protein YphG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,680,542	0	G	A	100.0%	124.2 / NA	36	A735V (GCG→GTG)	yphG	DUF5107 domain‑containing protein YphG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/8);  total (28/8)

CTCCGGCGACATCTCTACGCAACGTTGCCAAAAGGCAATGGCTTTGTTGTAGCTACGTTTGTTGTAGTAGAAGCAAGCTAGTAAATGGCGAGCAAACCAGCACTCTTCAATACTCTCCAGCGCCGCCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGGCAGCAAGCTGGCTTGCAGGTAAAGCGGCAGCGTGCGCTGGCAGTCCAG  >  NC_000913/2680418‑2680678                                                                                                                             |                                                                                                                                         cTCCGGCGACATCTCTACGCAACGTTGCCAAAAGGCAATGGCTTTGTTGTAGCTACGTTTGTTGTAGTAGAAGCAAGCTAGTAAATGGCGAGCAAACCAGCACTCTTCAATACTCTCCAGCGCCACCACTTCTTCCAGCg                                                                                                                           <  2:243625/140‑1 (MQ=255)            tctcTACGCAACGTTGCCAAAAGGCAATGGCTTTGTTGTAGCTACGTTTGTTGTAGTAGAAGCAAGCTAGTAAATGGCGAGCAAACCAGCACTCTTCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGaaa                                                                                                                <  1:626772/140‑1 (MQ=255)                      aCGTTGCCAAAAGGCAATGGCTTTGTTGTAGCTACGTTTGTTGTAGTAGAAGCAAGCTAGTAAATGGCGAGCAAACCAGCACTCTTCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACt                                                                                                      >  1:38215/1‑140 (MQ=255)                          tGCCAAAAGGCAATGGCTTTGTTGTAGCTACGTTTGTTGTAGTAGAAGCAAGCTAGTAAATGGCGAGCAAACCAGCACTCTTCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCgg                                                                                                  >  1:900286/1‑140 (MQ=255)                            ccAAAAGGCAATGGCTTTGTTGTAGCTACGTTTGTTGTAGTAGAAGCAAGCTAGTAAATGGCGAGCAAACCAGCACTCTTCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGa                                                                                                >  1:114926/1‑140 (MQ=255)                                             tgttgtAGCTACGTTTGTTGTAGTAGAAGCAAGCTAGTAAATGGCGAGCAAACCAGCACTCTTCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCtttt                                                                               <  2:114926/140‑1 (MQ=255)                                                  tAGCTACGTTTGTTGTAGTAGAAGCAAGCTAGTAAATGGCGAGCAAACCAGCACTCTTCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGAc                                                                          <  2:347132/140‑1 (MQ=255)                                                       aCGTTTGTTGTAGTAGAAGCAAGCTAGTAAATGGCGAGCAAACCAGCACTCTTCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGtt                                                                     >  1:442069/1‑140 (MQ=255)                                                       aCGTTTGTTGTAGTAGAAGCAAGCTAGTAAATGGCGAGCAAACCAGCACTCTTCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTAGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGtt                                                                     >  1:443628/1‑140 (MQ=255)                                                         gTTTGTTGTAGTAGAAGCAAGCTAGTAAATGGCGAGCAAACCAGCACTCTTCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCg                                                                   <  2:900286/140‑1 (MQ=255)                                                         gTTTGTTGTAGTAGAAGCAAGCTAGTAAATGGCGAGCAAACCAGCACTCTTCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCg                                                                   >  1:966088/1‑140 (MQ=255)                                                               tgtAGTAGAAGCAAGCTAGTAAATGGCGAGCAAACCAGCACTCTTCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGcc                                                                 <  2:502664/136‑1 (MQ=255)                                                               tgtAGTAGAAGCAAGCTAGTAAATGGCGAGCAAACCAGCACTCTTCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGcc                                                                 >  1:502664/1‑136 (MQ=255)                                                                  agtagAAGCAAGCTAGTAAATGGCGAGCAAACCAGCACTCTTCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCg                                                          >  1:845069/1‑140 (MQ=255)                                                                   gtagAAGCAAGCTAGTAAATGGCGAGCAAACCAGCACTCTTCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCgg                                                         >  1:397652/1‑140 (MQ=255)                                                                       aagcaagcTAGTAAATGGCGAGCAAACCAGCACTCTTCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCttt                                                     >  2:964763/1‑140 (MQ=255)                                                                              cTAGTAAATGGCGAGCAAACCAGCACTCTTCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGgcagc                                              >  1:585640/1‑140 (MQ=255)                                                                                 gTAAATGGCGAGCAAACCAGCACTCTTCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGGCAGCAAg                                           <  2:966088/140‑1 (MQ=255)                                                                                     aTGGCGAGCAAACCAGCACTCTTCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTCCGCCA‑CAAGCTGGc                                      >  2:565967/1‑140 (MQ=255)                                                                                           aGCAAACCAGCACTCTTCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGGCAGCAAGCTGGCTTGCa                                 >  1:882032/1‑140 (MQ=255)                                                                                                     cACTCTTCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGGCAGCAAGCTGGCTTGCAGGTAAAGCgg                       >  1:1070182/1‑140 (MQ=255)                                                                                                     cACTCTTCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGGCAGCAAGCTGGCTTGCAGGTAAAGCgg                       >  1:169773/1‑140 (MQ=255)                                                                                                          ttCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGGCAGCAAGCTGGCTTGCAGGTAAAGCGGCAGCg                  >  1:624451/1‑140 (MQ=255)                                                                                                          ttCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGGCAGCAAGCTGGCTTGCAGGTAAAGCGGCAGCg                  >  1:135030/1‑140 (MQ=255)                                                                                                          ttCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGGCAGCAAGCTGGCTTGCAGGTAAAGCGGCAGCg                  >  1:256563/1‑140 (MQ=255)                                                                                                          ttCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGGCAGCAAGCTGGCTTGCAGGTAAAGCGGCAGCg                  >  2:786077/1‑140 (MQ=255)                                                                                                              aTACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGGCAGCAAGCTGGCTTGCAGGTAAAGCGGCAGCGTgcg              >  2:1054900/1‑140 (MQ=255)                                                                                                               tACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGGCAGCAAGCTGGCTTGCAGGTAAAGCGGCAGCGTgcgc             >  1:555667/1‑140 (MQ=255)                                                                                                               tACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGGCAGCAAGCTGGCTTGCAGGTAAAGCGGCAGCGTgcgc             <  2:135030/140‑1 (MQ=255)                                                                                                                  tctcCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGGCAGCAAGCTGGCTTGCAGGTAAAGCGGCAGCGTGCGCTgg          >  2:712366/1‑140 (MQ=255)                                                                                                                  tctcCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGGCAGCAAGCTGGCTTGCAGGTAAAGCGGCAGCGTGCGCTgg          >  2:45322/1‑140 (MQ=255)                                                                                                                    tcCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGGCAGCAAGCTGGCTTGCAGGTAAAGCGGCAGCGTGCGCTGGCa        >  1:641634/1‑140 (MQ=255)                                                                                                                    tcCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGGCAGCAAGCTGGCTTGCAGGTAAAGCGGCAGCGTGCGCTGGCa        >  1:1147123/1‑140 (MQ=255)                                                                                                                    tcCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGGCAGCAAGCTGGCTTGCAGGTAAAGCGGCAGCGTGCGCTGGCa        >  2:257302/1‑140 (MQ=255)                                                                                                                    tcCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGGCAGCAAGCTGGCTTGCAGGTAAAGCGGCAGCGTGCGCTGGCa        >  1:319428/1‑140 (MQ=255)                                                                                                                      cAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGGCAGCAAGCTGGCTTGCAGGTAAAGCGGCAGCGTGCGCTGGCAGt      >  2:411707/1‑140 (MQ=255)                                                                                                                         cgccacCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGGCAGCAAGCTGGCTTGCAGGTAAAGCGGCAGCGTGCGCTGGCAGTCCa   >  2:372977/1‑140 (MQ=255)                                                                                                                          gccaccACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGGCAGCAAGCTGGCTTGCAGGTAAAGCGGCAGCGTGCGCTGGCAGTCCAg  <  2:1070182/140‑1 (MQ=255)                                                                                                                             |                                                                                                                                         CTCCGGCGACATCTCTACGCAACGTTGCCAAAAGGCAATGGCTTTGTTGTAGCTACGTTTGTTGTAGTAGAAGCAAGCTAGTAAATGGCGAGCAAACCAGCACTCTTCAATACTCTCCAGCGCCGCCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGGCAGCAAGCTGGCTTGCAGGTAAAGCGGCAGCGTGCGCTGGCAGTCCAG  >  NC_000913/2680418‑2680678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑