Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,830,289 T→C N159N (AAT→AAC)  gutQ → D‑arabinose 5‑phosphate isomerase GutQ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,830,2890TC100.0% 185.3 / NA 53N159N (AAT→AAC) gutQD‑arabinose 5‑phosphate isomerase GutQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/24);  total (29/24)

ATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTCCCACCCAGCCGGGGCACTGGGCGCTCGCTTGCTGAATAAAGTGCATCATCTGATGCGC  >  NC_000913/2830153‑2830424
                                                                                                                                        |                                                                                                                                       
aTAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAACAcc                                                                                                                                      <  1:762732/140‑1 (MQ=255)
        aTCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAACACCCTgatgat                                                                                                                              >  1:1036621/1‑140 (MQ=255)
         tCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAACACCCTGatgatg                                                                                                                             >  2:1139828/1‑140 (MQ=255)
           gcgcTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAACACCCTGATGATggg                                                                                                                           >  1:78823/1‑140 (MQ=255)
            cgcTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAACACCCTgatgat                                                                                                                              <  1:971174/136‑1 (MQ=255)
            cgcTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAACACCCTgatgat                                                                                                                              >  2:971174/1‑136 (MQ=255)
                 ctgGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAACACCCTGATGATGGGTGAcgc                                                                                                                     >  1:309038/1‑140 (MQ=255)
                   ggCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAACACCCTGATGATGGGTGACgcgc                                                                                                                   >  2:198394/1‑140 (MQ=255)
                         gACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCTACCTCCAGCACCGTCAACACCCTGATGATGGGTGACGCGCTGGCGa                                                                                                             <  1:730149/140‑1 (MQ=255)
                          aCCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAACACCCTGATGATGGGTGACGCGCTGGCGAt                                                                                                            <  2:1162708/140‑1 (MQ=255)
                              gCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAACACCCTGATGATGGGTGACGCGCTGGCGATGGCg                                                                                                        >  2:951298/1‑140 (MQ=255)
                              gCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAACACCCTGATGATGGGTGACGCGCTGGCGATGGCg                                                                                                        >  2:379346/1‑140 (MQ=255)
                              gCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAACACCCTGATGATGGGTGACGCGCTGGCGATGGCg                                                                                                        >  2:379347/1‑140 (MQ=255)
                                    cGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAACACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATg                                                                                                  >  1:1106862/1‑140 (MQ=255)
                                    cGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAACACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATg                                                                                                  <  1:845040/140‑1 (MQ=255)
                                       cGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAACACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAg                                                                                               >  1:1163112/1‑140 (MQ=255)
                                        gTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAACACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAgg                                                                                              <  1:173172/140‑1 (MQ=255)
                                             cGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAACACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGgcgcgc                                                                                         >  2:752224/1‑140 (MQ=255)
                                                    ccTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAACACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTa                                                                                  <  2:1163112/140‑1 (MQ=255)
                                                        gcggcAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAACACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATga                                                                              <  1:951298/140‑1 (MQ=255)
                                                               aaGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAACACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGAt                                                                       >  2:552557/1‑140 (MQ=255)
                                                                     tGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAACACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGcc                                                                 <  1:198394/140‑1 (MQ=255)
                                                                     tGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAACACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGcc                                                                 <  1:752224/140‑1 (MQ=255)
                                                                        tGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAACACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGc                                                              <  2:1036621/140‑1 (MQ=255)
                                                                         ggATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAACACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCt                                                             <  1:4651/140‑1 (MQ=255)
                                                                               ctcCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAACACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTcccacc                                                       >  2:443710/1‑140 (MQ=255)
                                                                               ctcCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAACACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTcccacc                                                       >  1:533082/1‑140 (MQ=255)
                                                                               ctcCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAACACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTcccacc                                                       >  2:529436/1‑140 (MQ=255)
                                                                                tcCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAACACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTcccaccc                                                      <  1:616869/140‑1 (MQ=255)
                                                                                  cGTAGAATGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAACACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTCCCACCCAg                                                    <  1:879794/140‑1 (MQ=255)
                                                                                   gTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAACACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTCCCACCCAGc                                                   <  1:529436/140‑1 (MQ=255)
                                                                                    tAGAACGCGAAGTCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAACACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTCCCACCCAGcc                                                  <  1:176787/140‑1 (MQ=255)
                                                                                        aCGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAACACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTCCCACCCAGCCgggg                                              <  1:552557/140‑1 (MQ=255)
                                                                                         cgcgAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAACACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTCCCACCCAGCCGGGGc                                             >  2:1118965/1‑140 (MQ=255)
                                                                                         cgcgAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAACACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTCCCACCCAGCCGGGGc                                             >  2:1166292/1‑140 (MQ=255)
                                                                                         cgcgAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAACACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTCCCACCCAGCCGGGGc                                             >  1:601895/1‑140 (MQ=255)
                                                                                                      cGATGCACCTTGCGCCGACCTCCAGCACCGTCAACACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTCCCACCCAGCCGGGGCACTGGGcgctcgc                                <  1:1166292/140‑1 (MQ=255)
                                                                                                        aTGCACCTTGCGCCGACCTCCAGCACCGTCAACACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTCCCACCCAGCCGGGGCACTGGGCGCTCGCtt                              <  2:309038/140‑1 (MQ=255)
                                                                                                        aTGCACCTTGCGCCGACCTCCAGCACCGTCAACACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTCCCACCCAGCCGGGGCACTGGGCGCTCGCtt                              <  1:254551/140‑1 (MQ=255)
                                                                                                         tGCACCTTGCGCCGACCTCCAGCACCGTCAACACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTCCCACCCAGCCGGGGCACTGGGCGCTCGCTTg                             <  2:78823/140‑1 (MQ=255)
                                                                                                          gCACCTTGCGCCGACCTCCAGCACCGTCAACACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTCCCACCCAGCCGGGGCACTGGGCGCTCGCTTGc                            >  2:35101/1‑140 (MQ=255)
                                                                                                                  cgcCGACCTCCAGCACCGTCAACACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTCCCACCCAGCCGGGGCACTGGGCGCTCGCTTGCTGAATaaa                    <  1:1051232/140‑1 (MQ=255)
                                                                                                                   gcCGACCTCCAGCACCGTCAACACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGATGATTTTGCCCGCTCCCACCCAGCCGGGGCACTGGGCGCTCGCTTGCTGAATAAAg                   >  1:335885/1‑140 (MQ=255)
                                                                                                                   gcCGACCTCCAGCACCGTCAACACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTCCCACCCAGCCGGGGCACTGGGCGCTCGCTTGCTGAATAAAg                   >  2:74885/1‑140 (MQ=255)
                                                                                                                               cACCGTCAACACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTCCCACCCAGCCGGGGCACTGGGCGCTCGCTTGCTGAATAAAGTGCATCATCTGa       >  2:385534/1‑140 (MQ=255)
                                                                                                                                  cGTCAACACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTCCCACCCAGCCGGGGCACTGGGCGCTCGCTTGCTGAATAAAGTGca               <  1:855580/129‑1 (MQ=255)
                                                                                                                                  cGTCAACACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTCCCACCCAGCCGGGGCACTGGGCGCTCGCTTGCTGAATAAAGTGca               >  2:855580/1‑129 (MQ=255)
                                                                                                                                   gTCAACACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTCCCACCCAGCCGGGGCACTGGGCGCTCGCTTGCTGAATAAAGTGCATCATCTGATgcg   >  2:443135/1‑140 (MQ=255)
                                                                                                                                   gTCAACACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTCCCACCCAGCCGGGGCACTGGGCGCTCGCTTGCTGAATAAAGTGCATCATCTGATgcg   >  1:972348/1‑140 (MQ=255)
                                                                                                                                    tCAACACCCTGATGATGGGTGACGCGCTGGCGATTGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTCCCACCCAGCCGGGGCACTGGGCGCTCGCTTGCTGAATAAAGTGca               <  1:665587/127‑1 (MQ=255)
                                                                                                                                    tCAACACCCTGATGATGGGTGACGCGCTGGCGATTGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTCCCACCCAGCCGGGGCACTGGGCGCTCGCTTGCTGAATAAAGTGca               >  2:665587/1‑127 (MQ=255)
                                                                                                                                    tCAACACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTCCCACCCAGCCGGGGCACTGGGCGCTCGCTTGCTGAATAAAGTGCATCATCTGATgcgc  >  2:431419/1‑140 (MQ=255)
                                                                                                                                    tCAACACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTCCCACCCAGCCGGGGCACTGGGCGCTCGCTTGCTGAATAAAGTGCATCATCTGATgcgc  >  2:33742/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                       
ATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTCCCACCCAGCCGGGGCACTGGGCGCTCGCTTGCTGAATAAAGTGCATCATCTGATGCGC  >  NC_000913/2830153‑2830424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑