Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,855,183	A→G	L282L (TTA→TTG)	fhlA →	DNA‑binding transcriptional activator FhlA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,855,183	0	A	G	100.0%	158.9 / NA	45	L282L (TTA→TTG)	fhlA	DNA‑binding transcriptional activator FhlA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/24);  total (21/24)

CTCCACTCACTATCTTGATAAACAGCATCCCGCCCACGAACAGAGCGAAGTCGATGAAGCCGGAACCCTCACCGAACGCGTGTTCAAAAGTAAAGAGATGCTGCTGATCAATCTCCACGAGCGGGACGATTTAGCCCCCTATGAACGCATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTA  >  NC_000913/2855051‑2855313                                                                                                                                     |                                                                                                                                   cTCCACTCACTATCTTGATAAACAGCATCCCGCCCACGAACAGAGCGAAGTCGATGAAGCCGGAACCCTCACCGAACGCGTGTTCAAAAGTAAAGAGATGCTGCTGATCAATCTCCACGAGCGGGACGATTTGGCCCCCt                                                                                                                             >  1:177594/1‑140 (MQ=255)  tCCACTCACTATCTTGATAAACAGCATCCCGCCCACGAACAGAGCGAAGTCGATGAAGCCGGAACCCTCACCGAACGCGTGTTCAAAAGTAAAGAGATGCTGCTGATCAATCTCCACGAGCGGGACGATTTGGCCCCCTa                                                                                                                            <  1:471166/140‑1 (MQ=255)      ctcactATCTTGATAAACAGCATCCCGCCCACGAACAGAGCGAAGTCGATGAAGCCGGAACCCTCACCGAACGCGTGTTCAAAAGTAAAGAGATGCTGCTGATTAATCTCCACGAGCGGGACGATTTGGCCCCCTATGaa                                                                                                                        >  1:670489/1‑140 (MQ=255)      ctcactATCTTGATAAACAGCATCCCGCCCACGAACAGAGCGAAGTCGATGAAGCCGGAACCCTCACCGAACGCGTGTTCAAAAGTAAAGAGATGCTGCTGATCAATCTCCACGAGCGGGACGATTTGGCCCCCTATGaa                                                                                                                        <  2:493443/140‑1 (MQ=255)      ctcactATCTTGATAAACAGCATCCCGCCCACGAACAGAGCGAAGTCGATGAAGCCGGAACCCTCACCGAACGCGTGTTCAAAAGTAAAGAGATGCTGCTGATCAATCTCCACGAGCGGGACGATTTGGCCCCCTATGaa                                                                                                                        >  1:656563/1‑140 (MQ=255)         actATCTTGATAAACAGCATCCCGCCCACGAACAGAGCGAAGTCGATGAAGCCGGAACCCTCACCGAACGCGTGTTCAAAAGTAAAGAGATGCTGCTGATCAATCTCCACGAGCGGGACGATTTGGCCCCCTATGAACGc                                                                                                                     >  1:366226/1‑140 (MQ=255)          ctATCTTGATAAACAGCATCCCGCCCACGAACAGAGCGAAGTCGATGAAGCCGGAACCCTCACCGAACGCGTGTTCAAAAGTAAAGAGATGCTGCTGATTAATCTCCACGAGCGGGACGATTTGGCCCCCTATGAACGCa                                                                                                                    <  2:670489/140‑1 (MQ=255)             tCTTGATAAACAGCATCCCGCCCACGAACAGAGCGAAGTCGATGAAGCCGGAACCCTCACCGAACGCGTGTTCAAAAGTAAAGAGATGCTGCTGATCAATCTCCACGAGCGGGACGATTTGGCCCCCTATGAACGCAtgt                                                                                                                 <  1:820865/140‑1 (MQ=255)                  aTAAACAGCATCCCGCCCACGAACAGAGCGAAGTCGATGAAGCCGGAACCCTCACCGAACGCGTGTTCAAAAGTAAAGAGATGCTGCTGATCAATCTCCACGAGCGGGACGATTTGGCCCCCTATGAACGCATGTTGTTc                                                                                                            <  1:219211/140‑1 (MQ=255)                   tAAACAGCATCCCGCCCACGAACAGAGCGAAGTCGATGAAGCCGGAACCCTCACCGAACGCGTGTTCAAAAGTAAAGAGATGCTGCTGATCAATCTCCACGAGCGGGACGATTTGGCCCCCTATGAACGCATGTTGTTCg                                                                                                           <  2:177594/140‑1 (MQ=255)                       cAGCATCCCGCCCACGAACAGAGCGAAGTCGATGAAGCCGGAACCCTCACCGAACGCGTGTTCAAAAGTAAAGAGATGCTGCTGATCAATCTCCACGAGCGGGACGATTTGGCCCCCTATGAACGCATGTTGTTCGacac                                                                                                       >  1:145928/1‑140 (MQ=255)                          cATCCCGCCCACGAACAGAGCGAAGTCGATGAAGCCGGAACCCTCACCGAACGCGTGTTCAAAAGTAAAGAGATGCTGCTGATCAATCTCCACGAGCGGGACGATTTGGCCCCCTATGAACGCATGTTGTTCGACACCTg                                                                                                    >  1:1121851/1‑140 (MQ=255)                           aacccGCCCACGAACAGAGCGAAGTCGATGAAGCCGGAACCCTCACCGAACGCGTGTTCAAAAGTAAAGAGATGCTGCTGATCAATCTCCACGAGCGGGACGATTTGGCCCCCTATGAACGCATGTTGTTCGACACCTgg                                                                                                   >  1:786670/3‑140 (MQ=255)                                 cccACGAACAGAGCGAAGTCGATGAAGCCGGAACCCTCACCGAACGCGTGTTCAAAAGTAAAGAGATGCTGCTGATCAATCTCCACGAGCGGGACGATTTGGCCCCCTATGAACGCATGTTGTTCGACACCTGGGGCAAc                                                                                             <  1:305240/140‑1 (MQ=255)                                 cccACGAACAGAGCGAAGTCGATGAAGCCGGAACCCTCACCGAACGCGTGTTCAAAAGTAAAGAGATGCTGCTGATCAATCTCCACGAGCGGGACGATTTGGCCCCCTATGAACGCATGTTGTTCGACACCTGGGGCAAc                                                                                             >  1:734487/1‑140 (MQ=255)                                  ccACGAACAGAGCGAAGTCGATGAAGCCGGAACCCTCACCGAACGCGTGTTCAAAAGTAAAGAGATGCTGCTGATCAATCTCCACGAGCGGGACGATTTGGCCCCCTATGAACGCATGTTGTTCGACACCTGGGGCAAcc                                                                                            <  2:656563/140‑1 (MQ=255)                                         cAGAGCGAAGTCGATGAAGCCGGAACCCTCACCGAACGCGTGTTCAAAAGTAAAGAGATGCTGCTGATCAATCTCCACGAGCGGGACGATTTGGCCCCCTATGAACGCATGTTGTTCGACACCTGGGGCAACCAGATTCa                                                                                     <  2:366226/140‑1 (MQ=255)                                            agCGAAGTCGATGAAGCCGGAACCCTCACCGAACGCGTGTCCAAAAGTAAAGAGATGCTGCTGATCAATCTCCACGAGCGGGACGATTTGGCCCCCTATGAACGCATGTTGTTCGACACCTGGGGCAAc                                                                                             <  2:223766/129‑1 (MQ=255)                                            agCGAAGTCGATGAAGCCGGAACCCTCACCGAACGCGTGTCCAAAAGTAAAGAGATGCTGCTGATCAATCTCCACGAGCGGGACGATTTGGCCCCCTATGAACGCATGTTGTTCGACACCTGGGGCAAc                                                                                             >  1:223766/1‑129 (MQ=255)                                                  gTCGATGAAGCCGGAACCCTCACCGAACGCGTGTTCAAAAGTAAAGAGATGCTGCTGATCAATCTCCACGAGCGGGACGATTTGGCCCCCTATGAACGCATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTtgtg                                                                            <  2:1139956/140‑1 (MQ=255)                                                  gTCGATGAAGCCGGAACCCTCACCGAACGCGTGTTCAAAAGTAAAGAGATGCTGCTGATCAATCTCCACGAGCGGGACGATTTGGCCCCCTATGAACGCATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTtgtg                                                                            <  1:988095/140‑1 (MQ=255)                                                  gTCGATGAAGCCGGAACCCTCACCGAACGCGTGTTCAAAAGTAAAGAGATGCTGCTGATCAATCTCCACGAGCGGGACGATTTGGCCCCCTATGAACGCATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTtatg                                                                            <  2:806630/140‑1 (MQ=255)                                                                  cccTCACCGAACGCGTGTTCAAAAGTAAAGAGATGCTGCTGATCAATCTCCACGAGCGGGACGATTTGGCCCCCTATGAACGCATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATg                                                            <  2:1121851/140‑1 (MQ=255)                                                                     tCACCGAACGCGTGTTCAAAAGTAAAGAGATGCTGCTGATCAATCTCCACGAGCGGGACGATTTGGCCCCCTATGAACGCATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCt                                                         >  1:410446/1‑140 (MQ=255)                                                                      cACCGAACGCGTGTTCAAAAGTAAAGAGATGCTGCTGATCAATCTCCACGAGCGGGACGATTTGGCCCCCTATGAACGCATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTg                                                        >  2:898766/1‑140 (MQ=255)                                                                       aCCGAACGCGTGTTCAAAAGTAAAGAGATGCTGCTGATCAATCTCCACGAGCGGGACGATTTGGCCCCCTATGAACGCATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTgg                                                       <  2:786670/140‑1 (MQ=255)                                                                         cGAACGCGTGTTCAAAAGTAAAGAGATGCTGCTGATCAATCTCCACGAGCGGGACGATTTGGCCCCCTATGAACGCATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCg                                                     <  2:923899/140‑1 (MQ=255)                                                                                     cAAAAGTAAAGAGATGCTGCTGATCAATCTCCACGAGCGGGACGATTTGGCCCCCTATGAACGCATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTgg                                         <  1:1158040/140‑1 (MQ=255)                                                                                         aGTAAAGAGATGCTGCTGATCAATCTCCACGAGCGGGACGATTTGGCCCCCTATGAACGCATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGt                                     <  1:579708/140‑1 (MQ=255)                                                                                            aaaGAGATGCTGCTGATCAATCTCCACGAGCGGGACGATTTGGCCCCCTATGAACGCATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCt                                  >  2:646198/1‑140 (MQ=255)                                                                                                  aTGCTGCTGATCAATCTCCACGAGCGGGACGATTTGGCCCCCTATGAACGCATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACt                            >  1:801696/1‑140 (MQ=255)                                                                                                   tgctgcTGATCAATCTCCACGAGCGGGACGATTTGGCCCCCTATGAACGCATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTg                           <  2:145928/140‑1 (MQ=255)                                                                                                     ctgctgATCAATCTCCACGAGCGGGACGATTTGGCCCCCTATGAACGCATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGgc                         >  1:605527/1‑140 (MQ=255)                                                                                                     ctgctgATCAATCTCCACGAGCGGGACGATTTGGCCCCCTATGAACGCATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGgc                         >  2:293916/1‑140 (MQ=255)                                                                                                     ctgctgATCAATCTCCACGAGCGGGACGATTTGGCCCCCTATGAACGCATGTTGTTCGACACCTGGGGCAACAAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGgc                         >  1:121406/1‑140 (MQ=255)                                                                                                       gctgATCAATCTCCACGAGCGGGACGATTTGGCCCCCTATGAACGCATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGgcgc                       >  1:85494/1‑140 (MQ=255)                                                                                                       gctgATCAATCTCCACGAGCGGGACGATTTGGCCCCCTATGAACGCATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGgcgc                       <  2:85494/140‑1 (MQ=255)                                                                                                        ctgATCAATCTCCACGAGCGGGACGATTTGGCCCCCTATGAACGCATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCa                      <  2:734487/140‑1 (MQ=255)                                                                                                           aTCAATCTCCACGAGCGGGACGATTTGGCCCCCTATGAACGCATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATg                   >  2:323494/1‑140 (MQ=255)                                                                                                           aTCAATCTCCACGAGCGGGACGATTTGGCCCCCTATGAACGCATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATg                   <  2:431999/140‑1 (MQ=255)                                                                                                             cAATCTCCACGAGCGGGACGATTTGGCCCCCTATGAACGCATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCg                 <  2:605527/140‑1 (MQ=255)                                                                                                                tctcCACGAGCGGGACGATTTGGCCCCCTATGAACGCATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAag              >  1:549884/1‑140 (MQ=255)                                                                                                                  tcCACGAGCGGGACGATTTGGCCCCCTATGAACGCATGTTGTTCGACACCTGGGGCAACAAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAagag            <  2:121406/140‑1 (MQ=255)                                                                                                                          cGGGACGATTTGGCCCCCTATGAACGCATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGtt    >  2:545458/1‑140 (MQ=255)                                                                                                                            ggACGATTTGGCCCCCTATGAACGCATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTa  >  1:444423/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                   CTCCACTCACTATCTTGATAAACAGCATCCCGCCCACGAACAGAGCGAAGTCGATGAAGCCGGAACCCTCACCGAACGCGTGTTCAAAAGTAAAGAGATGCTGCTGATCAATCTCCACGAGCGGGACGATTTAGCCCCCTATGAACGCATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTA  >  NC_000913/2855051‑2855313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑