Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	348,483	T→C	intergenic (‑40/‑199)	prpR ← / → prpB	DNA‑binding transcriptional dual regulator PrpR/2‑methylisocitrate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	348,483	0	T	C	100.0%	214.7 / NA	60	intergenic (‑40/‑199)	prpR/prpB	DNA‑binding transcriptional dual regulator PrpR/2‑methylisocitrate lyase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/22);  total (38/22)

CTCGAGGCTGATATCGCGAAACAGCTCGAACAGGCGCGTTACAGATACCGTCCAGATAACCGGTTTGTCGTCATTAAGCCGTGGTGGATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAGGCA  >  NC_000913/348348‑348615                                                                                                                                        |                                                                                                                                     cTCGAGGCTGATATCGCGAAACAGCTCGAACAGGCGCGTTACAGATACCGTCCAGATAACCGGTTTGTCGTCATTAAGCCGTGGTGGATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTg                                                                                                                                  <  1:730908/140‑1 (MQ=255) cTCGAGGCTGATATCGCGAAACAGCTCGAACAGGCGCGTTACAGATACCGTCCAGATAACCGGTTTGTCGTCATTAAGCCGTGGTGGATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATACTGGGTTTAGCCTTg                                                                                                                                  <  1:461050/140‑1 (MQ=255)     aGGCTGATATCGCGAAACAGCTCGAACAGGCGCGTTACAGATACCGTCCAGATAACCGGTTTGTCGTCATTAAGCCGTGGTGGATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTc                                                                                                                              >  1:32317/1‑140 (MQ=255)                 cgAAACAGCTCGAACAGGCGCGTTACAGATACCGTCCAGATAACCGGTTTGTCGTCATTAAGCCGTGGTGGATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCa                                                                                                                  >  2:942970/1‑140 (MQ=255)                 cgAAACAGCTCGAACAGGCGCGTTACAGATACCGTCCAGATAACCGGTTTGTCGTCATTAAGCCGTGGTGGATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCa                                                                                                                  <  2:814648/140‑1 (MQ=255)                         cTCGAACAGGCGCGTTACAGATACCGTCCAGATAACCGGTTTGTCGTCATTAAGCCGTGGTGGATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAAcg                                                                                                          >  1:765038/1‑140 (MQ=255)                            gAACAGGCGCGTTACAGATACCGTCCAGATAACCGGTTTGTCGTCATTAAGCCGTGGTGGATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCgg                                                                                                       >  2:622438/1‑140 (MQ=255)                                   cgcgTTACAGATACCGTCCAGATAACCGGTTTGTCGTCATTAAGCCGTGGTGGATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAAcg                                                                                                          <  2:298588/130‑1 (MQ=255)                                   cgcgTTACAGATACCGTCCAGATAACCGGTTTGTCGTCATTAAGCCGTGGTGGATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAAcg                                                                                                          >  1:298588/1‑130 (MQ=255)                                   cgcgTTACAGATACCGTCCAGATAACCGGTTTGTCGTCATTAAGCCGTGGTGGATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACa                                                                                                >  2:236946/1‑140 (MQ=255)                                         aCAGATACCGTCCAGATAACCGGTTTGTCGTCATTAAGCCGTGGTGGATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCt                                                                                          <  1:236946/140‑1 (MQ=255)                                            gATACCGTCCAGATAACCGGTTTGTCGTCATTAAGCCGTGGTGGATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACAt                                                                                               <  2:372051/132‑1 (MQ=255)                                            gATACCGTCCAGATAACCGGTTTGTCGTCATTAAGCCGTGGTGGATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACAt                                                                                               >  1:372051/1‑132 (MQ=255)                                            gATACCGTCCAGATAACCGGTTTGTCGTCATTAAGCCGTGGTGGATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCt                                                                                          <  1:1107256/137‑1 (MQ=255)                                            gATACCGTCCAGATAACCGGTTTGTCGTCATTAAGCCGTGGTGGATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCt                                                                                          >  2:1107256/1‑137 (MQ=255)                                            gATACCGTCCAGATAACCGGTTTGTCGTCATTAAGCCGTGGTGGATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGaa                                                                                       >  1:977680/1‑140 (MQ=255)                                                ccGTCCAGATAACCGGTTTGTCGTCATTAAGCCGTGGTGGATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGa                                                                                        >  1:330010/1‑135 (MQ=255)                                                ccGTCCAGATAACCGGTTTGTCGTCATTAAGCCGTGGTGGATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGa                                                                                        <  2:330010/135‑1 (MQ=255)                                                 cGTCCAGATAACCGGTTTGTCGTCATTAAGCCGTGGTGGATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGtt                                                                                  >  2:555137/1‑140 (MQ=255)                                                  gTCCAGATAACCGGTTTGTCGTCATTAAGCCGTGGTGGATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTa                                                                                 >  1:37682/1‑140 (MQ=255)                                                    ccAGATAACCGGTTTGTCGTCATTAAGCCGTGGTGGATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAAc                                                                               >  1:159008/1‑140 (MQ=255)                                                    ccAGATAACCGGTTTGTCGTCATTAAGCCGTGGTGGATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAAc                                                                               >  2:350371/1‑140 (MQ=255)                                                    ccAGATAACCGGTTTGTCGTCATTAAGCCGTGGTGGATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAAc                                                                               >  2:613105/1‑140 (MQ=255)                                                       gATAACCGGTTTGTCGTCATTAAGCCGTGGTGGATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGa                                                                            >  1:413574/1‑140 (MQ=255)                                                            ccGGTTTGTCGTCATTAAGCCGTGGTGGATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGc                                                                       >  2:453854/1‑140 (MQ=255)                                                                   gtcgtcATTAAGCCGTGGTGGATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTg                                                                <  2:1002055/140‑1 (MQ=255)                                                                   gtcgtcATTAAGCCGTGGTGGATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTg                                                                <  1:613105/140‑1 (MQ=255)                                                                   gtcgtcATTAAGCCGTGGTGGATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTg                                                                <  1:361029/140‑1 (MQ=255)                                                                      gtcATTAAGCCGTGGTGGATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCgg                                                             >  2:476161/1‑140 (MQ=255)                                                                      gtcATTAAGCCGTGGTGGATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCgg                                                             >  1:613326/1‑140 (MQ=255)                                                                       tcATTAAGCCGTGGTGGATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGa                                                            <  2:413574/140‑1 (MQ=255)                                                                        cATTAAGCCGTGGTGGATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGAt                                                           <  1:622438/140‑1 (MQ=255)                                                                         aTTAAGCCGTGGTGGATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGAtt                                                          <  1:476161/140‑1 (MQ=255)                                                                              gCCGTGGTGGATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTc                                                     <  1:966384/140‑1 (MQ=255)                                                                                cGTGGTGGATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCAt                                                   <  2:839612/140‑1 (MQ=255)                                                                                  tggtggATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGa                                                 >  1:998274/1‑140 (MQ=255)                                                                                  tggtggATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACACATATTTGCGGATTAGTTCATGa                                                 >  1:549462/1‑140 (MQ=255)                                                                                   ggtggATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGAc                                                >  1:96283/1‑140 (MQ=255)                                                                                    gtggATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGTAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGAt                                                           >  1:789304/1‑128 (MQ=255)                                                                                    gtggATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGTAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGAt                                                           <  2:789304/128‑1 (MQ=255)                                                                                         tgtgCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTAtc                                          >  2:553957/1‑140 (MQ=255)                                                                                             ccATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTa                                      >  1:1108512/1‑140 (MQ=255)                                                                                                 aGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACaa                                  >  1:824103/1‑140 (MQ=255)                                                                                                 aGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACaa                                  >  1:1020639/1‑140 (MQ=255)                                                                                                   cgcACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAAt                                >  2:203181/1‑140 (MQ=255)                                                                                                   cgcACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAAt                                <  2:1020639/140‑1 (MQ=255)                                                                                                     cACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAAt                                >  2:114076/1‑138 (MQ=255)                                                                                                     cACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAAt                                <  1:114076/138‑1 (MQ=255)                                                                                                     cACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTg                              >  2:760457/1‑140 (MQ=255)                                                                                                     cACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTg                              >  1:888131/1‑140 (MQ=255)                                                                                                     cACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTg                              >  1:291913/1‑140 (MQ=255)                                                                                                        cGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGaaa                           >  2:1157175/1‑140 (MQ=255)                                                                                                        cGCAAAGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGaaa                           >  1:1002908/1‑140 (MQ=255)                                                                                                           aaaGTTAAGAAACCGAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTa                        <  2:827867/140‑1 (MQ=255)                                                                                                                         gAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAACa                    >  2:1027828/1‑130 (MQ=255)                                                                                                                         gAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAACa                    <  1:1027828/130‑1 (MQ=255)                                                                                                                         gAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAACATTTAATTtta          >  1:661972/1‑140 (MQ=255)                                                                                                                         gAATATTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAACATTTAATTtta          >  1:206186/1‑140 (MQ=255)                                                                                                                             atTGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTaa      <  2:977680/140‑1 (MQ=255)                                                                                                                              ttGGGTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAg     <  1:1157175/140‑1 (MQ=255)                                                                                                                                 ggTTTAGCCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAGGCa  >  1:766715/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                     CTCGAGGCTGATATCGCGAAACAGCTCGAACAGGCGCGTTACAGATACCGTCCAGATAACCGGTTTGTCGTCATTAAGCCGTGGTGGATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAGGCA  >  NC_000913/348348‑348615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑