Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,966,994	C→T	A481T (GCG→ACG)	ptsP ←	phosphoenolpyruvate‑protein phosphotransferase PtsP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,966,994	0	C	T	100.0%	219.0 / NA	62	A481T (GCG→ACG)	ptsP	phosphoenolpyruvate‑protein phosphotransferase PtsP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/32);  total (30/32)

AATCCCACGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAGTTTTTCTTCATGTTCCGGGCTTAAACCAGCATTGAGCATGACTTT  >  NC_000913/2966860‑2967129                                                                                                                                       |                                                                                                                                        aaTCCCACGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGTCACCt                                                                                                                                    >  1:1124857/1‑140 (MQ=255)       aCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGTCAcc                                                                                                                                     >  2:126907/1‑133 (MQ=255)       aCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGTCAcc                                                                                                                                     <  1:126907/133‑1 (MQ=255)       aCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGTCACCTGttctt                                                                                                                              >  1:614183/1‑140 (MQ=255)         gCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCCTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGTGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGTCACCTGttcttct                                                                                                                            >  2:606431/1‑140 (MQ=255)              cccAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGa                                                                                                                       >  1:616318/1‑140 (MQ=255)                   gCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGa                                                                                                                  <  2:37760/140‑1 (MQ=255)                   gCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGa                                                                                                                  <  2:833385/140‑1 (MQ=255)                   gCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGa                                                                                                                  <  1:625490/140‑1 (MQ=255)                          ttCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACt                                                                                                           <  1:437665/140‑1 (MQ=255)                               ttCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTgca                                                                                                      <  2:132989/140‑1 (MQ=255)                               ttCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTgca                                                                                                      <  1:563373/140‑1 (MQ=255)                                 cGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTgcagc                                                                                                    >  1:583224/1‑140 (MQ=255)                                   cTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCAt                                                                                                  >  2:219043/1‑140 (MQ=255)                                   cTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCAt                                                                                                  <  2:370395/140‑1 (MQ=255)                                      aTCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGaa                                                                                               <  2:168367/140‑1 (MQ=255)                                      aTCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGaa                                                                                               <  1:688977/140‑1 (MQ=255)                                      aTCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGaa                                                                                               <  2:832793/140‑1 (MQ=255)                                       tCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAAt                                                                                              >  2:987291/1‑140 (MQ=255)                                       tCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAAt                                                                                              >  1:23857/1‑140 (MQ=255)                                       tCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAAt                                                                                              >  2:900807/1‑140 (MQ=255)                                              gTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGAttt                                                                                       <  2:23857/140‑1 (MQ=255)                                                     aGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCg                                                                                >  2:893897/1‑140 (MQ=255)                                                     aGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCg                                                                                >  1:1145061/1‑140 (MQ=255)                                                       ctgctTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGAttt                                                                                       >  2:699957/1‑131 (MQ=255)                                                       ctgctTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGAttt                                                                                       <  1:699957/131‑1 (MQ=255)                                                        tgctTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATa                                                                             >  1:27685/1‑140 (MQ=255)                                                                 gCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGAt                                                                    <  2:811001/140‑1 (MQ=255)                                                                 gCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGTCACCTGTCCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGAt                                                                    >  1:25581/1‑140 (MQ=255)                                                                  cTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGAt                                                                    >  1:808889/1‑139 (MQ=255)                                                                  cTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGAt                                                                    <  2:808889/139‑1 (MQ=255)                                                                    ccGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGcc                                                                 >  1:741735/1‑140 (MQ=255)                                                                    ccGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGcc                                                                 <  2:1145061/140‑1 (MQ=255)                                                                    ccGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGcc                                                                 <  1:219043/140‑1 (MQ=255)                                                                                    gCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGccc                                                 <  2:27685/140‑1 (MQ=255)                                                                                         gTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAGttt                                            >  2:779368/1‑140 (MQ=255)                                                                                         gTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAGttt                                            <  1:893897/140‑1 (MQ=255)                                                                                                  ttATCATTGAACATTTGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAGTTTTTCTTCATg                                   >  1:151156/1‑140 (MQ=255)                                                                                                  ttATCATTGAACATTTGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAGTTTTTCTTCATg                                   >  2:71017/1‑140 (MQ=255)                                                                                                   tATCATTGAACATTTGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAGTTTTTCTTCATGt                                  >  2:1169962/1‑140 (MQ=255)                                                                                                      cATTGAACATTTGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAGTTTTTCTTCATGTTcc                               <  2:151156/140‑1 (MQ=255)                                                                                                      cATTGAACATTTGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAGTTTTTCTTCATGTTcc                               <  1:59647/140‑1 (MQ=255)                                                                                                      cATTGAACATTTGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAGTTTTTCTTCATGTTcc                               >  2:639565/1‑140 (MQ=255)                                                                                                           aaCATTTGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCTGTTTTTCTTCATGTTCCGGGCt                          >  1:827160/1‑140 (MQ=255)                                                                                                           aaCATTTGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAGTTTTTCTTCATGTTCCGGGCt                          >  2:343196/1‑140 (MQ=255)                                                                                                           aaCATTTGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAGTTTTTCTTCATGTTCCGGGCt                          <  2:5165/140‑1 (MQ=255)                                                                                                           aaCATTTGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAGTTTTTCTTCATGTTCCGGGCt                          <  2:158958/140‑1 (MQ=255)                                                                                                           aaCATTTGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAGTTTTTCTTCATGTTCCGGGCt                          <  2:741735/140‑1 (MQ=255)                                                                                                           aaCATTTGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGAAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCTTCAATACGGCTGCCCAGTTTTTCTTCATGTTCCGGGCt                          >  2:1043822/1‑140 (MQ=255)                                                                                                             cATTTGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAGTTTTTCTTCATGTTCCGGGCTTa                        <  1:779368/140‑1 (MQ=255)                                                                                                               tttGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAGTTTTTCTTCATGTTCCGGGCTTaaa                      <  1:769606/140‑1 (MQ=255)                                                                                                                ttGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAGTTTTTCTTCATGTTCCGGGCTTAAAc                     >  1:1114724/1‑140 (MQ=255)                                                                                                                ttGCAGCATCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAGTTTTTCTTCATGATCCGGGCTTAAAc                     >  2:462996/1‑140 (MQ=255)                                                                                                                     gcaTCCCCTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAGTTTTTCTTCATGTTCCGGGCTTAAACCAGCa                <  1:71017/140‑1 (MQ=255)                                                                                                                          cccTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAGTTTTTCTTCATGTTCCGGGCTTAAACCAGCATTGAg           <  1:158391/140‑1 (MQ=255)                                                                                                                           ccTGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAGTTTTTCTTCATGTTCCGGGCTTAAACCAGCATTGAGc          >  1:1121926/1‑140 (MQ=255)                                                                                                                             tGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAGTTTTTCTTCATGTTCCGGGCTTAAACCAGCATTGAGCAt        <  2:998980/140‑1 (MQ=255)                                                                                                                             tGATACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAGTTTTTCTTCATGTTCCGGGCTTAAACCAGCATTGAGCAt        <  2:1114724/140‑1 (MQ=255)                                                                                                                               aTACTGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAGTTTTTCTTCATGTTCCGGGCTTAAACCAGCATTGAGCATGa      <  1:639565/140‑1 (MQ=255)                                                                                                                                   tGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAGTTTTTCTTCATGTTCCGGGCTTAAACCAGCATTGAGCAt        >  2:774822/1‑134 (MQ=255)                                                                                                                                   tGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAGTTTTTCTTCATGTTCCGGGCTTAAACCAGCATTGAGCAt        <  1:774822/134‑1 (MQ=255)                                                                                                                                   tGCGTCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAGTTTTTCTTCATGTTCCGGGCTTAAACCAGCATTGAGCATGACttt  >  1:171524/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        AATCCCACGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAGTTTTTCTTCATGTTCCGGGCTTAAACCAGCATTGAGCATGACTTT  >  NC_000913/2966860‑2967129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑