Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,978,212	C→T	V230I (GTT→ATT)	lysA ←	diaminopimelate decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,978,212	0	C	T	100.0%	268.5 / NA	76	V230I (GTT→ATT)	lysA	diaminopimelate decarboxylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (40/36);  total (40/36)

CGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAG  >  NC_000913/2978075‑2978347                                                                                                                                          |                                                                                                                                        cgcTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAg                                                                                                                                       >  1:109548/1‑140 (MQ=255)      ccAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGc                                                                                                                                  >  2:842919/1‑140 (MQ=255)         ggAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCa                                                                                                                               >  1:141704/1‑140 (MQ=255)                aCCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCAccgcccg                                                                                                                        <  2:215019/140‑1 (MQ=255)                aCCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCAccgcccg                                                                                                                        >  2:1036614/1‑140 (MQ=255)                aCCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCAccgcccg                                                                                                                        >  1:861319/1‑140 (MQ=255)                aCCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCAccgcccg                                                                                                                        >  2:599937/1‑140 (MQ=255)                aCCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATCTGCTCACGCGCGGCATTCCACAGACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCAccgcccg                                                                                                                        >  1:86722/1‑140 (MQ=255)                aCCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATCTGCTCACGCGCGGCATTCCACAGACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCAccgcccg                                                                                                                        <  2:86722/140‑1 (MQ=255)                    ggTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGa                                                                                                                    >  2:336202/1‑140 (MQ=255)                         aaTTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAg                                                                                                               <  1:778467/140‑1 (MQ=255)                         aaTTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAg                                                                                                               <  1:630608/140‑1 (MQ=255)                             cccAGTTCCACGGGGTGGCCAAAAGGGGGGGCGTTTTGCTCCCGCGGGGCTTCCCCCAGCCCATAATAATTTCGGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTg                                                                                                           <  2:861319/139‑1 (MQ=255)                                aGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTaa                                                                                                        >  2:1026084/1‑140 (MQ=255)                                aGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTaa                                                                                                        >  1:914202/1‑140 (MQ=255)                                   ttCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGACCATAATAATTTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATc                                                                                                     <  2:141704/140‑1 (MQ=255)                                     cacaGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCt                                                                                                   >  1:772389/1‑140 (MQ=255)                                       caGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGa                                                                                                 <  1:336202/140‑1 (MQ=255)                                              gCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGa                                                                                                 >  1:1126765/1‑133 (MQ=255)                                              gCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGa                                                                                                 <  2:1126765/133‑1 (MQ=255)                                              gCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAAtt                                                                                          >  1:267881/1‑140 (MQ=255)                                              gCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAAtt                                                                                          >  1:924053/1‑140 (MQ=255)                                               cccAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGACCATAATAATGTTCGGTATCAACCGCCTCTTGACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTc                                                                                         >  1:189096/1‑140 (MQ=255)                                                ccAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCg                                                                                        >  1:1005929/1‑140 (MQ=255)                                                 cAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGa                                                                                       <  2:787462/140‑1 (MQ=255)                                                 cAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGa                                                                                       <  1:538907/140‑1 (MQ=255)                                                   aaTGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGACCAAAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATg                                                                                     <  1:599937/140‑1 (MQ=255)                                                    aTGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGa                                                                                    >  2:1029249/1‑140 (MQ=255)                                                      ggcgggcgATTTGCCCCCGCGCGGCATCCCACAGACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGAcc                                                                                  <  2:1137495/140‑1 (MQ=255)                                                           gcgTTTTGCTCACGCGCGACATTCCACAGACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACg                                                                             <  2:687803/140‑1 (MQ=255)                                                           gcgATTTGCTCACGCGCGGCATTCCACAGACCATAATAATGTTCGGTATCACCCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACg                                                                             <  1:1026084/140‑1 (MQ=255)                                                           gcgATTTGCTCACGCGCGGCATTCCACAGACCATAATAATGTTCGGTATCAACCGCCTCTTGACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACg                                                                             <  2:189096/140‑1 (MQ=255)                                                           gcgATTTGCTCACGCGCGGCATTCCACAGACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACg                                                                             <  2:634380/140‑1 (MQ=255)                                                                     cACGCGCGGCATTCCACAGACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGca                                                                   <  2:213009/140‑1 (MQ=255)                                                                         cgcgGCATTCCACAGACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGcaccac                                                               >  2:213436/1‑140 (MQ=255)                                                                         cgcgGCATTCCACAGACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGcaccac                                                               <  2:948793/140‑1 (MQ=255)                                                                           cgGCATTCCACAGACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACcacac                                                             >  2:582005/1‑140 (MQ=255)                                                                            ggCATTCCACAGACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACcacaca                                                            <  2:712921/140‑1 (MQ=255)                                                                            gccATCCCACAGACCAAAATAATTTTCGTTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACcacaca                                                            <  2:269000/138‑1 (MQ=255)                                                                             gCATTCCACAGACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCacacac                                                           <  2:875103/140‑1 (MQ=255)                                                                              cATTCCACAGACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACAcc                                                          <  2:914202/140‑1 (MQ=255)                                                                                   cacaGACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTc                                                     >  2:1160736/1‑140 (MQ=255)                                                                                       gACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGa                                                 <  1:1036614/140‑1 (MQ=255)                                                                                       gACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGa                                                 <  2:1005929/140‑1 (MQ=255)                                                                                        aCCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGAt                                                >  2:422970/1‑140 (MQ=255)                                                                                        aCCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCATGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCacacac                                                           <  2:619642/129‑1 (MQ=255)                                                                                        aCCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCATGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCacacac                                                           >  1:619642/1‑129 (MQ=255)                                                                                          cATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTg                                                        <  2:994547/130‑1 (MQ=255)                                                                                          cATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTg                                                        >  1:994547/1‑130 (MQ=255)                                                                                           ataataATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATggg                                             >  2:1100233/1‑140 (MQ=255)                                                                                             aataatGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCa                                           >  2:335066/1‑140 (MQ=255)                                                                                                  tGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATc                                      >  2:313851/1‑140 (MQ=255)                                                                                                    ttCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCaa                                    >  2:466671/1‑140 (MQ=255)                                                                                                      cGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACg                                  >  1:257292/1‑140 (MQ=255)                                                                                                       ggTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGc                                 >  2:1031100/1‑140 (MQ=255)                                                                                                        gTATCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGcc                                >  2:721345/1‑140 (MQ=255)                                                                                                           tCAACCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGa                             <  1:842919/140‑1 (MQ=255)                                                                                                             aaCCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAAc                           <  1:466671/140‑1 (MQ=255)                                                                                                             aaCCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAAc                           <  2:267881/140‑1 (MQ=255)                                                                                                              aCCGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAAcc                          >  2:552300/1‑140 (MQ=255)                                                                                                               ccGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCa                         <  1:1029249/140‑1 (MQ=255)                                                                                                                cGCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCaa                        <  1:213436/140‑1 (MQ=255)                                                                                                                 gCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAAt                       <  1:1031100/140‑1 (MQ=255)                                                                                                                 gCCTCTTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAAt                       <  1:552300/140‑1 (MQ=255)                                                                                                                   ctctTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCa                         >  2:267423/1‑136 (MQ=255)                                                                                                                   ctctTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCa                         <  1:267423/136‑1 (MQ=255)                                                                                                                   ctctTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAAtgt                     >  2:581041/1‑140 (MQ=255)                                                                                                                   ctctTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAAtgt                     >  2:910565/1‑140 (MQ=255)                                                                                                                    tctTCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAAtgtg                    <  1:761096/140‑1 (MQ=255)                                                                                                                      ttCACCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCa                  >  2:726952/1‑140 (MQ=255)                                                                                                                         aCCCTGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCAtgt               <  1:592284/140‑1 (MQ=255)                                                                                                                             tGTTGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAAt           >  2:407888/1‑140 (MQ=255)                                                                                                                               ttGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGc         >  1:1010843/1‑140 (MQ=255)                                                                                                                                tGATAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGcc        >  2:569175/1‑140 (MQ=255)                                                                                                                                  aTAAGGAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGa      >  2:171919/1‑140 (MQ=255)                                                                                                                                      ggAATAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAg  >  2:766659/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        CGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAG  >  NC_000913/2978075‑2978347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑